XB-CLONE-3288255
Clone Name: IMAGE:7670071
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Gene:
prdm8
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136202 [+]
GCTCCTGATTGTGTTTACTCCTTCCTGCCCCCTCCCATTCATCTGCTTTTGCAATCTCTCCATCTACAAAGAGCAGGCAAAGCGACAGCAACATCATTCT
GCCTTATCATGGAAATACAGTGACACAATACAAGGGACACAAGAAAAGCAAGAGCAACACCCTGTCTCCGGGTCTTGTATCCATACGACTAGTCACAGAT
TCTGGACATGTCAATCATGGAAGAAGCTGGCGTCCAGAAACCTGTTTGGGATGGAGATGCCAAAGCTGTTCAGCAATGCCTAACGGATATTTTTACAAGT
GTATATACCACCTGCGATATCCCTGAAAATGCCATTTTTGGCCCCTGTGTGCTGAGTCACACTTCCCTGTACGACAGCATAGCTTTCATCGCTCTCAAGT
CTACAGACAAGAGGACTGTACCCTACATATTCAGGGTGGACACTTCAGCTGCAAATGGCTCCTCCGAGGGACTAATGTGGCTCCGTCTGGTTCAGTCAGC
AAGGAACAAAGAAGAGCAGAATTTGGAAGCCTACATCAAAAACGGGCAGTTGTTTTATAGATCACTGCGTAGGGTTGCTAAAGACGAGGAACTCTTGGTT
TGGTATGGAAAGGAACTGACTGACCTTCTACTTCTGAGCCCCACAAGCAGGACCCCTGTAAAAAACACAGTTTCATCCCAATCAATATGCCTGGAGTGCA
ACCAGAAGTTTCAGTTTGAATTCCCCTTTGTGGCTCACGTAAGGTTCCGATGCCCCAAAAGACTCCAAGGCTCGGATTCGAATTTAGAACACGCAAGCAA
GGACAACAGAGAGTTGGAGCCTCGAAACACCATGTCAGCTTCAGCCAAATACAACAAACTGACCTTCTATCAACCTCAAAAGGAAAACCACAAACCTACA
ACAGACTTTCACAATCTGGCAAGGGACATGGAAAATGTCAGGGGATCTTCCTCTTCATTCAGTTCCTTGCCTAACAAGGCAAACTTGACACGGCAAGACC
CAGATTCCACGCCTGATGGCCTTCACAGAGCCAAGAGGAAATATGATGAAAATCAAGAAGAAAGTAGATCTAAAAAGAGTGATGTCTCTGGTAGGTTCTT
GGACAGACCAATACCTTCCTCTAAAGAGGATTTAGTTTGTAGCCCTCAGCCTTTCAGAACCAGCTCCTATTATAATTTGGAAGAAAGTGGACAGTTATTT
GCACCACCTAGTCCAGAAACAGGGGAAGCCAAAAGAAGCGCCTTTGTAGAGGTAAAAAAAAACCTCTAGAGGAGTTGAAGACCTTAACGCTGAGCATAAA
TCCATGTCCCCATCCCATTCCAATAGTCCAAGTGAGCCTAAGCGGCTGCAGGTGAGACCAGAGAACCAGGCTGGGGAACGTTCCCCTATAACTAGTGCAT
TCAGCAGCGTACCCCAGATGACAAACCTGGAGCAGGAGAGAAAAAGCGCATTCTCCCAACCAACTAGAAGTGGCTTCAATCACATTTCACCTCTGCAGGT
CATGGGTCAAAAAATAATATCCAGTGGAATTAATTTAGATTGTCCAGGAGAAAACGTGGGTACAGCTAGGCTCTTCCAACCTGATCCTCTGTCAGTGAAG
CTTCAGAGCCCGGAAATAAATGGCAACTGCACACTACAGGGCAGCTTGCCCAAACAAAGTCCTTTCCTCTTTGCCACCACGTTCTGGCCCAAAAGTTCAG
CAGGTCCTCTCCAGCTGCAGATGCCCTCAACTCTCACCTTGTTACCCCCATCCTTCACTTCCCTCTGCTTGCCAGCGCAAAACTGGTGTGCCAAATGTAA
TGCGTCCTTCAGAATGACCTCGGATTTGGTATATCACATGAGGTCCCACCACAAAAAGGAATATGCAATGGAGCCTCTAGTAAAGAGGAGGAGGGAAGAG
AAGCTAAAGTGCCCCATTTGCAATGAGTCCTTCAGGGAACGCCACCACCTGTCCAGGCATATGACCTCTCATAACTAGGTCTGTGTTATACCACAAGTGA
TGGACCTCTAAATACTCCCACAGTTTTACGCTGGGGCATGAGCTTTGAACGCAAATGTGTGAGGGTATTTTCCTCTCTAAATGCACGCATTTTCACTTTC
CAAAAGTACATTTTTAAAAATGTCATATATATTGCAGCATATTGATGCATTTGTCATATCTTTTTACTTAAATTATTAAGCACTTATGGTTTAGGTGTAA
TATCAATAATTATATGTATTTTTTTGTTGTTATGATTTTTTCCTATTTTGGATAACAAAGATTATATGGGTGGGCATAGTTCCTTTGTGTAATTCCTCAC
AACATTCGTGTTCCTACCAGATCAGTATAAATATTATTATTACAGAGGACTCTCTAGTTATGGAGGAGATGCATGGACAATACATCACCGCATAATCATG
GACTCCAAATAGAACCTCATCAACTTGTTTCCTATCAATGCAAAAAACATGTTATATTGTGACAGTGAAATGAAGTATAATCCCCTTTTCTTTGTTTTTT
TCTGTCCCACCATCCCGATTTGGAAAAGAAGGGATTTATTACACTTTATAGGTTATCTGGGTATGTTGGATGCTTTGACAATAAATGACTTTATTTTCTG
CAAAATGAAAAAAAAAAAAAAA
5' EST: CX876903 [+]ctcctgattgtgtttactccttcctgccccctcccattcatctgcttttgcaatctctccatctacaaagagcaggcaaagcgacagcaacatcattctg
ccttatcatggaaatacagtgacacaatacaagggacacaagaaaagcaagagcaacaccctgtctccgggtcttgtatccatacgactagtcacagatt
ctggacatgtcaatcatggaagaagctggcgtccagaaacctgtttgggatggagatgccaaagctgttcagcaatgcctaacggatatttttacaagtg
tatataccacctgcgatatccctgaaaatgccatttttggcccctgtgtgctgagtcacacttccctgtacgacagcatagctttcatcgctctcaagtc
tacagacaagaggactgtaccctacatattcagggtggacacttcagctgcaaatggctcctccgagggactaatgtggctccgtctggttcagtcagca
aggaacaaagaagagcagaatttggaagcctacatcaaaaacgggcagttgttttatagatcactgcgtagggttgctaaagacgaggaactcttggttt
ggtatggaaaggaactgactgaccttctacttctgagccccacaagcaggacccctgtaaaaaacacagtttcatcccaatcaatatgcctggagtgcaa
ccagaagtttcagtttgaattcccctttgtggctcacgtaaggttccgatgccccaaaagactccaaggctcggattcgaatttagaacacgcaagcaag
gacaacagagagttggagcctcgaaacaccatgtcagcttcagc
3' EST: CX876902 [+]gcagaaaataaagtcatttattgtcaaagcatccaacatacccagataacctataaagtgtaataaatcccttcttttccaaatcgggatggtgggacag
aaaaaaacaaagaaaaggggattatacttcatttcactgtcacaatataacatgttttttgcattgataggaaacaagttgatgaggttctatttggagt
ccatgattatgcggtgatgtattgtccatgcatctcctccataactagagagtcctctgtaataataatatttatactgatctggtaggaacacgaatgt
tgtgaggaattacacaaaggaactatgcccacccatataatctttgttatccaaaataggaaaaaatcataacaacaaaaaaatacatataattattgat
attacacctaaaccataagtgcttaataatttaagtaaaaagatatgacaaatgcatcaatatgctgcaatatatatgacatttttaaaaatgtactttt
ggaaagtgaaaatgcgtgcatttagagaggaaaataccctcacacatttgcgttcaaagctcatgccccagcgtaaaactgtgggagtatttagaggtcc
atcacttgtggtataacacagacctagttatgagaggtcatatgcctggacaggtggtggcgttccctgaaggactcattgcaaatggggcactttagct
tctcttccctcctcctctttactagaggctccattgcatattcctttttgtggtgggacctcatgtgatataccaaatccgaagtcattctgaaggacgc
attacatttggcacaccagtt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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