XB-CLONE-3287817
Clone Name: IMAGE:7670607
|
Gene:
rprm
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX877645 [+]
cagggcccctctttcacctactatatcaccctcataactctcatcagcatctcactggccctacagatcgtcattggtatcctactcatcatcattgctc
gtaagaacctgaatgacgtttccaagcagccgcagctcaatgtgctgaataacgcggccacgggattggtcttcctcactgtgctgatcaatatcttcat
cacggcttttggggtgcaaaaaagtggtctgtaccccgccagggggtagggcttcctacagatgccaggtaatctaagccgatgtcggagtctgatcctc
gctcgtcagcgttcccctctctcccgctcagcttcctctgcgattaacccatatctatcaacatatgtactagtgaatgaggggcgcccagttatattta
ttgtattagacatattaactagaagcctcagtaccaaattataggagctgcctataaaaccccagtgctgatgggctgattgctaagctgacggatagat
agagcttgaatggaaggggtgagtaactggtgcggtatcggggattttacagccgtctgtaataagcctgggcctagggcagtacaaatttaggggcgac
atgccgccctactgccccgaggatgctgggagttgcaactatgcatttcacatgcattccatatgttgaagctgcttctcagtcagtgccccaatggggc
tccctctacatcctggactcacccccagcagtcttctatagttctatccctgcagggaacatataacaggcgctgtaacaattaactttcctattctgtg
cgactgggtcctgatggcaaatgagtgaaagcaat
3' EST: CX877644 [+]ctttacataggtttttatttatttatagccccccttgtaaaataaaaggaaatgtaaaccttctaaacaatgtaaaattgctgagagtgctcttctgagc
acatttacatttgacatttactgctgatagaatggcactctgtaaataaaatatacatacgtgaatgtatatacaggtatgggtcccgttatccagaatg
ctcaggataaggggtctttccgtaatttggatctccataacttaagtctgttaaaaatcttttaaatactgaataaacccaatagggctgttctgccccc
aataaggggtaattatatcttagttgggatcaagtacaggtactgttttattattacagagaaaagggaatcattaaaccatgaaataaacccaatagga
ctgttctgcccccaataaggggtaattatatcttagttgggatcaagtacaggtactgttttattattacagagaaaagggaatcatttaaccatgaaat
aaacccaatagggctgttctgcccccaataaggggtaattatatcttagttgggatcaagtacaggtactgttttattattacagagaaaagggaatcat
ttaaccattaaataaacccaatagggctgttctgcccccaataaggggtaattatatcttagttgggatcaagtacaggtactgttttattattacagag
aaaagggaatcatttaaccatta
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: