XB-CLONE-3287705
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Clone Name: IMAGE:7670590
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Gene:
tifa
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136230 
[+]
GCTGCGTAAGGAGCAAAACAAGCAATTTAAAGGACACATACAGGACACTGCCGACAGGTTACAAATATTAGAAAATTACCTGTTTAATCAGATAAAGGAA
ACCACTTCCTGGGATCCATGGAGTGCTGAGAGTTGAGGGGAGGGGAGGAGATGAGCAGGAGAAGAGCAGGGGAAGAGCAGGGTGTAGTTGATGGTGGGAG
GAGCCAGAGGCAGTCCTATATTCCTAATTCTAGAGCGGTGCCTCAGTGCTGCTGATAACAGGCTGCCTGGCTGTGCATAAGCAAATAAACCCGTGTGATC
AGCTGACTGCAAGAAGGAGCAAACACAAGAAATGGATGAAACATTAAATGTGGACACAGAGCAGACGCTCACTTGTTTAACCCTCACCATGTATCATCCA
AATCACCAAGATCAAAAACTCTTCAGAGGCATCAATTTTAGCCGTAAAGAAGAAATAAAAGCAGATGCGGTTGTGGCCTTTGGACGCGACTATAATGTTT
GCCGCTATCCTTTGCTTAGTAACAGAGTCTCCCGGATTCAATTCAACTTGCAGTTCTTCAAGCATTTCAACTGCTCCACTACCGCCATTGAGATAAAGAA
CTTAAGCAAAAAAAACAAACTTTATGTGGACAACCTGGAATTGGATTATCTCAACAAGATTGAACTTCCACCAAAGTGTATGATCCGCTTCGGAGACTTT
CAGATATTAGCAGTAATCGAAAGAGGAGACTGTGATGAGAAGTTTGAAATATGCTGTGAAAAATCCCACGTGTCCCTTGTTCAGGACAGTTTTATACCAA
CTATGCAGCCAATTCCAGAATGTGGAACCCTAAATGCTGTTGAAATTGATGAGAATGAATATTAAATTTGTAACTTAAAATTCTATACCACAAAATGGTG
GTGTAACTAGAAAGGCTACCAGGCCAATTCATTGTAGGCCCCTTCCCTTAACTTTGGAAATAAGACATTTTTTCATAAACATGTAAGGATTATCGGTCCC
CAAGGGACCCCCCCCCCCCCCCCAACAGCTGCAGGGGCTGCCACCTCTATAGTTATGTACTCATAACAGATTGTTGTCATGAGATGCTAGAGTCAGATGG
AGCTGGCAGAGGCACATTCAAGTAAATGGAAAGGCCACTGACAACACTGCCTTCAGTAATTAGTGTGCTATTTGTGTATTGACTCCAAATGCAGTTTTTA
GAAGTTTATGCAACACTGTGTCAAAATACTCCCGGGAAACCTATCCTGCCAAACAGTATTTTAATTAAAAAAAAAAAAAAA
5' EST: CX877857 [+]ctgcgtaaggagcaaaacaagcaatttaaaggacacatacaggacactgccgacaggttacaaatattagaaaattacctgtttaatcagataaaggaaa
ccacttcctgggatccatggagtgctgagagttgaggggaggggaggagatgagcaggagaagagcaggggaagagcagggtgtagttgatggtgggagg
agccagaggcagtcctatattcctaattctagagcggtgcctcagtgctgctgataacaggctgcctggctgtgcataagcaaataaacccgtgtgatca
gctgactgcaagaaggagcaaacacaagaaatggatgaaacattaaatgtggacacagagcagacgctcacttgtttaaccctcaccatgtatcatccaa
atcaccaagatcaaaaactcttcagaggcatcaattttagccgtaaagaagaaataaaagcagatgcggttgtggcctttggacgcgactataatgtttg
ccgctatcctttgcttagtaacagagtctcccggattcaattcaacttgcagttcttcaagcatttcaactgctccactaccgccattgagataaagaac
ttaagcaaaaaaaacaaactttatgtggacaacctggaattggattatctcaacaagattgaacttccaccaaagtgtatgatccgcttcggagactttc
agatattagcagtaatcgaaagaggagactgtgatgagaagtttgaaatatgctgtgaaaaatcccacgtgtcccttgttcaggacagttttataccaac
tatgcagccaattccagaatgtggaa
3' EST: CX877856 [+]aaaatactgtttggcaggataggtttcccgggagtattttgacacagtgttgcataaacttctaaaaactgcatttggagtcaatacacaaatagcacac
taattactgaaggcagtgttgtcagtggcctttccatttacttgaatgtgcctctgccagctccatctgactctagcatctcatgacaacaatctgttat
gagtacataactatagaggtggcagcccctgcagctgttggggggggggggggggtcccttggggaccgataatccttacatgtttatgaaaaaatgtct
tatttccaaagttaagggaaggggcctacaatgaattggcctggtagcctttctagttacaccaccattttgtggtatagaattttaagttacaaattta
atattcattctcatcaatttcaacagcatttagggttccacattctggaattggctgcatagttggtataaaactgtcctgaacaagggacacgtgggat
ttttcacagcatatttcaaacttctcatcacagtctcctctttcgattactgctaatatctgaaagtctccgaagcggatcatacactttggtggaagtt
caatcttgttgagataatccaattccaggttgtccacataaagtttgttttttttgcttaagttctttatctcaatggcggtagtgg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
