XB-CLONE-3287478
Clone Name: IMAGE:7670699
|
Gene:
gask1a
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX878275 [+]
caagaataccatgccaagaccagtttaggatttaatgaagacgcgccaccatggtttacatcagatgacctgaaaaaaatgaggttattgtcagaaggcg
aggttgttgcaaaatctcgaattcctgcacatggacaagttttgcaggtgggtttatgtcctcttgctgagaattgtcagcaagggaagatttgttcaaa
aggactttgtggattaataaagcgtccaaccgatttatatgaagtcctggcttttcaccttgacaggattttagggttaaacaggagcctgccagcagtg
ggccgaaaatttacaagcgatttactgccctacaagtataccgacggtgccccaaggccaatcatatggtgggctccagacattaagcacttaaatgaca
caaacaatgatcaaaactctcatgcactggactggatacaataccagcagatgttgaagcacaggtgtggcatgggggactcttcagtgaatattaatag
acctccttgcctgggcatcaagcatacggagtgggcaatgctggctctgtttgattttctcctgcaggtccaggatcgactagaccgctattgctgcggc
tttaaccccgacccatcagagccctgtgtggaggaactgctgcatgacaaatgccgtaatgcaaaaggacttgttttagtgcacattttggtgcgtcaga
gtaacccatcacatttagtttacattgacaacgcgggacgaccccatcaccctga
3' EST: CX878274 [+]gcacattgtagtctttattttacaaataaacattttacagttataaagattattatgttagtggtttgatggtaattatacctgtgtttttcttcttttt
atttgcaatacaaggaaatgaaaaaggttaaaaaagtttgtgctattgcaatccccctggccaaggaaacactaaagactaccttaaaaaaagctagtat
acagaagctgtacgcaactgaagtgatcctccaattagatgcgacccataattatctgcctagtggcctgacattccgtggctgctatggggcaactcac
tttagcatttctcagtatcagagagaaaaaacaggcagggttttaaaaacatgcagccagactggcaatatttttcatgtttttggacatgcacaccgac
agtagtttaatatttttctagaaaaaaattgctgtattctgtattagaatagttcaaaagtataaaggggactttgttcaggttttagctgagatttgag
ctcaacatttatgtcaggcttttttacccatttatgaactatggctcccattctcctgcttcgtggaaattgcagtttaaagcaaaagtttacataacaa
ccagtcatgccacaaattagtacctgctccacaggaagttcaaaataaagctgtaactattgcactgttcccccataactcattagtagcattaaggccc
agaaggtctgctgttataagtcaaagaaggaaattcttgcttagcccatctctcttacacaatgcataagcagatttccattgtcctgagttttgtattt
acaaatgtagggagaaagtccaagt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: