XB-CLONE-3286622
Clone Name: IMAGE:7671494
|
Gene:
rragd
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC118790 [+]
GCTGTGGTGCCTGGGAGCTGCGCTAGAGCTGTCCGGGTTGGAAACAAGTGCAGGGCGGTGAGGGACGATGAGCCTACCAGGGAAAAGCCATGAGCGACAG
CAGGAGGAGGAAGGGGATGACGAGGACGATGATATAATGGGGGTGTCGGATTATGGAGATGGAGACAGCTTCATGGATGGGGAGCGAGGAAGTGAAGGAG
ATGACGAAGTTTTGGATTTTACTGATCCCTTCAGCACAGAGGTTAAGCCAAGAATTCTGTTGATGGGCCTAAGAAGAAGTGGAAAATCTTCAATTCAAAA
GGTGGTCTTTCATAAAATGTCACCAAATGAAACACTCTTCTTAGAAAGCACAAACAAGATCTGCAGAGAAGATGTCTCCAATAGCTCATTTGTAAACTTT
CAGATTTGGGACTTCCCAGGGCAGATTGATTTCTTCGACCCAACATTTGACTATGAGATGATTTTCAGAGGAACAGGGGCACTAATATTTGTAATTGATT
CACAGGATGATTACATGGAAGCACTGGCCAGATTACACTTGACCGTGACAAGTGCATACAAAGTAAACCCAGACATCAACTTTGAGGTTTTTATTCATAA
AGTTGATGGCCTGTCAGATGATCACAAGATAGAAACACAAAGAGATATCCATCAGAGAGCAAACGATGACCTTGTAGATGCAGGACTGGAGAAGATCCAT
TTGAGCTTTTATCTCACCAGCATATATGATCATTCAATATTTGAAGCTTTCAGCAAAGTGGTACAAAAACTAATTCCACAACTTCCAACCTTGGAGAATC
TTCTGAACATCTTTATATCAAACTCTGGGATCGAGAAGGTTTTCCTGTTTGACGTGGTTAGTAAAATCTATATAGCCACAGACAGTTCTCCAGTGGACAT
GCAGACCTACGAACTATGTTGTGATATGATTGACGTGGTTATTGACATCTCCTGCATCTATGGGCTGGAAGGAGCTGGGACTCCATATGACAAGGAATCA
CTGGCAATCATAAAACTAAACAACACTACAGTGCTGTACTTAAAGGAGGTGACAAAGTTCTTAGCTCTTGTTTGCTTTGTACGAGAAGAGAGTTTCGAGA
GAAAAGGGTTGATAGACTACAATTTCCATTGCTTTCGAAAGGCTATTCAGGAGGTGTTTGAAGTGAGGGTTAAAGTACTGCGATCTCGAAAACACCAGAG
TCAGACAAAGAAGAGCAGGAGAGCAACTCCCAATGGAACACCAGGAGTGCCATTATAGGCATGGAGAGGAGCAGAATGCAAATGATGAAACATAAAACTT
ATCTTCCTGCACTAATGGTCAAGCACACTTAACAGTTCAGTTAATTACTCACTTTGCCAGTTTTATTACTTGTCTTTGGCCCAAAAAAAAAAAAAAA
5' EST: CX879800 [+]ctgtggtgcctgggagctgcgctagagctgtccgggttggaaacaagtgcagggcggtgagggacgatgagcctaccagggaaaagccatgagcgacagc
aggaggaggaaggggatgacgaggacgatgatataatgggggtgtcggattatggagatggagacagcttcatggatggggagcgaggaagtgaaggaga
tgacgaagttttggattttactgatcccttcagcacagaggttaagccaagaattctgttgatgggcctaagaagaagtggaaaatcttcaattcaaaag
gtggtctttcataaaatgtcaccaaatgaaacactcttcttagaaagcacaaacaagatctgcagagaagatgtctccaatagctcatttgtaaactttc
agatttgggacttcccagggcagattgatttcttcgacccaacatttgactatgagatgattttcagaggaacaggggcactaatatttgtaattgattc
acaggatgattacatggaagcactggccagattacacttgaccgtgacaagtgcatacaaagtaaacccagacatcaactttgaggtttttattcataaa
gttgatggcctgtcagatgatcacaagatagaaacacaaagagatatccatcagagagcaaacgatgaccttgtagatgcaggactggagaagatccatt
tgagcttttatctcaccagcatatatgatcattncatatttgaagctttcagncaagtggtacanaaactaattccacaactt
3' EST: CX879799 [+]tttttttttttttttgggccaaagacaagtaataaaactggcaaagtgagtaattaactgaactgttaagtgtgcttgaccattagtgcaggaagataag
ttttatgtttcatcatttgcattctgctcctctccatgcctataatggcactcctggtgttccattgggagttgctctcctgctcttctttgtctgactc
tggtgttttcgagatcgcagtactttaaccctcacttcaaacacctcctgaatagcctttcgaaagcaatggaaattgtagtctatcaacccttttctct
cgaaactctcttctcgtacaaagcaaacaagagctaagaactttgtcacctcctttaagtacagcactgtagtgttgtttagttttatgattgccagtga
ttccttgtcatatggagtcccagctccttccagcccatagatgcaggagatgtcaataaccacgtcaatcatatcacaacatagttcgtaggtctgcatg
tccactggagaactgtctgtggctatatagattttactaaccacgtcaaacaggaaaaccttctcgatcccagagtttgatataaagatgttcagaagat
tctccaaggttggaagttgtggaattagtttttgtaccactttgctgaaagcttcaaatattgaatgatcatatatgctggtgagataaaagctcaaatg
gatcttctccagtcctgcatctacaaggtcatcgtttgctctctgatggatatctctttgtgtttctatct
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: