XB-CLONE-3284429
Sequence:
Full length sequence: BC158288 [+]
cgcagcgatacgcgactcaggatgcagctcctgaaattaacagcgacttccctcctcctcctgtgcctgattcctctgcctgcagtgctggcagacagct
gcgcaccctacatcggttccgattggcgttaccacccaaaacaagattgtctgctgagcttttgtgcaggagaatgtctacaaaggtattgcagtatctt
ccctgggacccagcttgaccagtcccagattctgtgcattataaacaatttttggatggttcttggattcggcatccttgtaggcctctcaatcataggt
ggaattatctcttgcctctgcaaatggtattggcttgtctcccaacgctccagtcataccaacaccaatgtggcagtgaccaatgtcattccccagcagc
cagtgatgccccttacaggataccaaccagttgcaacccagaatatgcatgttggttatgcagctaaaaacccgccagcttacccagggcatgaaaatca
aacttttatggagtactcttaaagtcctggttttgccagttgcttaatatgttaccactgatttgtccaaaggatgaagaaaaagagatccactctctga
gtgccaagggttgccttgcaaccatatgagtgcatattatgttacaagttatgctttagagacttagtagcattctgcagtttagaattacagaaaaatt
ctatactttatgtgtttactctaatagacattctgtgccattcctagccaaaaaacacctttattatttgtactgtttataatattgttccatttgtttt
accacattagggttcactgatacaatataaagcattatgttcattttcttgctgcagggaaataaacatttcgcagcatgccaaaataattatctgtcca
tacacaaactgacttgctacatggggtaacaccaggcctggactgggattcaaaatagaccttggcatttcaagtacacagaggctcaaacagcccccca
tcaggccagtatatagtaactgtctatggtaccttatagcaggccctttggcatttgcctgaatccacagattgccagtctgggcctgggtcacacactg
tatggcccccatccttttgttctgaccattcatgcaaagagcctgaataactggaaaagcagaaaataaaaaggacctgctgttccccctcacttcctca
tatgtcgatgctccatgacctgaccgccatagttcatcagcagattaaattttcaaaggtgccctttaactgaactgtccaatattcatagtgcatggac
atcagttgggatcagcaattttatctgtgcacgtatggcccccttaagtgtcgctgtcacctttcacaggagcaagagatgttgtatatgttctttgtag
cctcatgctgaagatgcacagccgacccagagtctctacagcaggggtgggcaaattacggcctgcgggccatgtccggcacgttggtctttttaatctg
gcccgcggaggattggtgtgtctcgcttgtcccaactctgcaagtctcatcacgcgagatgtgggtcaaggctgcatgtaagcgctggcccaacccgtct
gtcaaattttaaaagtcaatgtggcccctgagcctaaatgtttgcccacccctgctctacaggaagcagctggaactagagaaacacgggcaacctgtag
ccccagctacaacacccacagctgaagcctgccaggacaactacttctgggccagttcatcataagatcaaaaggcaaaaaggaatcaaacagctccagg
cagcatttgaaaactgcagtgaatttaatggcagtgtcagcactttttgccttttgatcttatgatatactgttggggtggctcacagacacctggaatc
tgcagccgtatactactgattatttgtgctgaactataactcaaaaaaaacatttctgggccagtttccttactgataaaaatattggctgttacatgta
actccctgcagtgtttgtggtgcacttccttgcttatcttggagtattgggacttttctgttacagaagtattgtgttttgtttttaataaaattacttt
ttttcaaaaaaaaaaaaaaa
5' EST: CX883592 [+]cgcagcgatacgcgactcaggatgcagctcctgaaattaacagcgacttccctcctcctcctgtgcctgattcctctgcctgcagtgctggcagacagct
gcgcaccctacatcggttccgattggcgttaccacccaaaacaagattgtctgctgagcttttgtgcaggagaatgtctacaaaggtattgcagtatctt
ccctgggacccagcttgaccagtcccagattctgtgcattataaacaatttttggatggttcttggattcggcatccttgtaggcctctcaatcataggt
ggaattatctcttgcctctgcaaatggtattggcttgtctcccaacgctccagtcataccaacaccaatgtggcagtgaccaatgtcattccccagcagc
cagtgatgccccttacaggataccaaccagttgcaacccagaatatgcatgttggttatgcagctaaaaacccgccagcttacccagggcatgaaaatca
aacttttatggagtactcttaaagtcctggttttgccagttgcttaatatgttaccactgatttgtccaaaggatgaagaaaaagagatccactctctga
gtgccaagggttgccttgcaaccatatgagtgcatattatgttacaagttatgctttagagacttagtagcattctgcagtttagaattacagaanaatt
ctatactttatgtgtttactctaatagacattctgtgccattcctagccaaaaaacaccttttata
3' EST: CX883591 [+]tttttttttttttttgaaaaaaagtaattttattaaaaacaaaacacaatacttctgtaacagaaaagtcccaatactccaagataagcaaggaagtgca
ccacaaacactgcagggagttacatgtaacagccaatatttttatcagtaaggaaactggcccagaaatgttttttttgagttatagttcagcacaaata
atcagtagtatacggctgcagattccaggtgtctgtgagccaccccaacagtatatcataagatcaaaaggcaaaaagtgctgacactgccattaaattc
actgcagttttcaaatgctgcctggagctgtttgattcctttttgccttttgatcttatgatgaactggcccagaagtagttgtcctggcaggcttcagc
tgtgggtgttgtagctggggctacaggttgcccgtgtttctctagttccagctgcttcctgtagagcaggggtgggcaaacatttaggctcaggggccac
attgacttttaaaatttgacagacgggttgggccagcgcttacatgcagccttgacccacatctcgcgtgatgagacttgcagagttgggacaagcgaga
cacaccaatcctccgcgggccagattaaaaagaccaacgt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: