XB-CLONE-3283188
Clone Name: IMAGE:7793729
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Gene:
cyp19a1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC118823 [+]
GTGCCTCACAGTGAGCTCACACAATGCTGAAATCATAGAAAAAGCCGTGTTGCTCCTATTCCAAGTGTCAAGGCCATTCTACCATATGCAGTTGCCACGT
GTAAAATCAAAGGAACTCTACGTGGGATGCCAAGTGCCCTATCCTTTGTGGATGAAACTTTATCTACTTGTTTTGACTCTTTTCACTTCTATCTAGCAGA
TTCTCACAATATAAAGAGGTTCTTTCTTTTCTTGCATAGGCGAGCAGGGCCTTGACAAGCCGTGATTATGGAAGCCTTGAATCCCGTGCAGTATAACAGC
ACAGAGGCTGTTCCTACTCTCGCACCTGCCACTACGGTTTCTCTCCTGCTCTTCATTTTTTTGCTTATCATTCTATGGAATCAAGAGGAGACCTGTCTGA
TACCAGGCCCAGCCTATTGCATGGGGCTTGGGCCCCTCATTTCTTATGGCCGTTTTCTACTGACAGGAATTGGCAAAGCAGCAAATTACTACAACAACAT
GTACGGGGAATTTGTCAGAGTCTGGATTAATGGCGAGGAAACACTGGTAATCAGCAAAGCTTCAGCAACTTTTCACATTATGAAGCACAGCCATTATATC
TCACGTTTTGGAAGCAAACTGGGGCTGCAGTGTATTGGCATGAATGAAAACGGTATCATATTTAATAGCAACCCATCTCTATGGAAGGTCATCCGACCAT
TCTTCATCAAAGCTTTGTCTGGTCCAGGACTTATGCAAACCACAGAAATCTGTATAAGATCGACAAAACGCTACCTGGATAACCTTGGCAATGTGACAAA
TGAACTGGGAAATGTAGATGTCCTTAAACTAATGAGGCTTATTATGTTAGATACATCAAATAATCTCTTCTTAAGGATACCTTTAGATGAAAATGAAATT
GTTCTTAAAATCCAGAAATACTTTGACGCCTGGCAGGCTCTGCTTCTGAAACCAGACATCTTCTTTAAAATTTCCTGGCTGTACAAGAAATATGAAAAAT
CGGCAAATGATCTGAAGGAGGCTATTGAAATTCTCATTGAACAAAAAAGACAGAAACTCTCAAGTTCTGAAAAGCTGGATGAAAATATGGATTTTGCATC
AGAACTTATTTTTGCCCAGAATCATGGAGATCTAACAGCTGAAAATGTCAATCAGTGTATTTTGGAAATGCTGATAGCTGCTCCCGATACCATGTCTGTA
TCTCTCTTCTTCATGTTGGTTCTGGTTGCTCAACACCCAAAGATAGAAGAAGGAATAATGAATGAAATTGATAATGTTATTGGTGATCGGGATGTAGAGA
GCAACGACATTCCAAATCTCAAAGTTCTGGAGAATTTTATTTATGAGAGCATGAGGTACCAACCGGTTGTAGACCTGGTTATGCGCAAGGCCCTGGAGGA
CGATATGATTGATGGTTACTATGTGAAGAAAGGCACTAACATCATATTAAACTTGGGGCGCATGCACAGAATCGAATACTTTCCAAAGCCGAATGAGTTC
ACTTTGGAAAACTTTGAAAAGACGGTTCCATATCGTTACTTTCAGCCCTTTGGCTCTGGCCCACGTGCTTGTGCGGGGAAGTACATAGCTATGGTTATGA
TGAAAGTCATTCTGGTTACTCTTTTCAAGAGGTACAAGGTGCAGACACTGGGAGGAAGATGCTTGGAGAACATCCAAAACAACAATGATTTGTCTATGCA
CCCTGATGAAAGCCAGCCATGCTTAGAGATGATTTTCATTCCCAAGAACACAGCAGAGCTCAAACAGTAACAGATAATATAATCCCATATGTCATATCTG
GAAATTACAAATGTTCTTAGCATAGTGACAGGTGACAGTGTTGGTCTGTGTTAAACGTTGATTTAACCACTGCTGTTGTATGCTCTTTGTAACTATCAGG
CACTTAGTAGTAGTGCAACAATCATTAATGTTAGGGTTCTCATTCTTTCTGCTGTAGAGTTGTTAATGGTGGGATTTGAAGTTCAGCAGCTTTAAAGGAT
GAGTAAAAGCAACACATGTAAATGCCCCGGTGCATTGTAAACATAATTCTCATTCAGCAGTTTGAAAAAGCATGATATCTATATTTGAAAATACTTCTGG
GAAGCAGAAGTGAATATGTATTATGTAAGGCTTCATAGAACACATTGAACAGGGCAGGGCTTTATTTAGACAACAAATAAAAGATGGCAGAGCTGATGAT
AAACACTGCAATAAAACAAACAATTAAGAAAATATCTGAAAATGTGAAGAATAGATATAAATTTGTTATAATAAAACTAATAATAATAATAATTACTTTT
TTTGTTACTGGTCTTTTAAGGCTGTTATATATATTCCTGTTAAGTACAGTGATATTGTTATTGTTTATATTTATCAGTATTATTAATAATAAAATATGCC
TATATTTAAAAAAAAAAAAAAA
5' EST: CX885719 [+]tgcctcacagtgagctcacacaatgctgaaatcatagaaaaagccgtgttgctcctattccaagtgtcaaggccattctaccatatgcagttgccacgtg
taaaatcaaaggaactctacgtgggatgccaagtgccctatcctttgtggatgaaactttatctacttgttttgactcttttcacttctatctagcagat
tctcacaatataaagaggttctttcttttcttgcataggcgagcagggccttgacaagccgtgattatggaagccttgaatcccgtgcagtataacagca
cagaggctgttcctactctcgcacctgccactacggtttctctcctgctcttcatttttttgcttatcattctatggaatcaagaggagacctgtctgat
accaggcccagcctattgcatggggcttgggcccctcatttcttatggccgttttctactgacaggaattggcaaagcagcaaattactacaacaacatg
tacggggaatttgtcagagtctggattaatggcgaggaaacactggtaatcagcaaagcttcagcaacttttcacattatgaagcacagccattatatct
cacgttttggaagcaaactggggctgcagtgtattggcatgaatgaaaacggtatcatatttaatagcaacccatctctatggaaggtcatccgaccatt
cttcatcaaagctttgtctggtccaggacttatgcaaaccacagaaatctgtataagatcgacaaaacgctacctggataaccttggcaatgtgacaaat
gaactggggaaattgtagatgt
3' EST: CX885718 [+]tttttttttttttttaatataggcatattttattattaataatactgataaatataaacaataacaatatcactgtacttaacaggaatatatataacag
ccttaaaagaccagtaacaaaaaaagtaattattattattattagttttattataacaaatttatatctattcttcacattttcagatattttcttaatt
gtttgttttattgcagtgtttatcatcagctctgccatcttttatttgttgtctaaataaagccctgccctgttcaatgtgttctatgaagccttacata
atacatattcacttctgcttcccagaagtattttcaaatatagatatcatgctttttcaaactgctgaatgagaattatgtttacaatgcaccggggcat
ttacatgtgttgcttttactcatcctttaaagctgctgaacttcaaatcccaccattaacaactctacagcagaaagaatgagaaccctaacattaatga
ttgttgcactactactaagtgcctgatagttacaaagagcatacaacagcagtggttaaatcaacgtttaacacagaccaacactgtcacctgtcactat
gctaagaacatttgtaatttccagatatgacatatgggattatattatctgttactgtttgagctctgctgtgttcttgggaatgaaaatcatctctaag
catggctggctttcatcagggtgcatagacaaatcattgttgttttggatgttctccaagcatcttcctcccagtgtctgcaccttgtacctcttgaaaa
gagtaaccagaatgactttcatcataaccatagctatgtacttccccgca
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: