XB-CLONE-3282742
Clone Name: IMAGE:7674908
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Gene:
klf4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC125671 [+]
ATTCTCCAGCTCTGGTGACTCGAAGGGAACCTGAGGAATTTAATGAGCTTCTGGACTTTGATTTTATCTTGTCCAATTCCCTTATCCACCAAGAGTCCAT
GACTGTGGGTTCTTCTTCTTCATCTTCGTCGGTCACTGCTTCCTCACCTCCACCTCCTGCCACACTTCCTGATACAATGTCTGCTCTCTCGTCTACCACT
TGCAACTTTGTATACCAGGTACGTTGTCCCCAGGAATCATCAGGGGGCACCCTTATGTACAATGCAAGGGATCCTACTATGTCCAGTGCCACTTTCAACC
TGGCGGATATAAATGATGTTTCACCTTCAGGGGGTTTTGTGGCTGAACTGATGAGACCTGACTTGGACCCAGTATTTATGCAACAACAGCCACAAGCTAG
CTTGCAAGGTAAATTTGTTCTAAAGACAACTGTTAGCATGGGTGATTATGGCCAAACCATCAGTGTTAGGAAAAACAACTCTGGCATAGACAGTCCCTTG
TCTTACACTACTCACCAGCTTCCTAGAGTGTGCCAAAAGATCAAGCAGGAACCTTCTTCATCTTGCACTTCAGCATGCCCAATGGATGGACAGGGCCAAC
AGTCACAAAGCATGCGTGAGTTTCAAGCAGGAAGGGGTCTACCAATTAGGACTAACCAATCTTTGTCCCCAGAAGAGCTGATGGGCAGAGACTGTCATTC
TTCCTCTCAGACATTAAGTCATTCTTCATTGCCAATACCTCCAGCCTACCACACCTCTGCCAGTTTTTCCCACTTCACTTCAGATCAGCCGCAGACACAA
TTGCCTCCAACCCAGCTACAATATCAAGGTCAGTCTTATCTAAATATTTTTGAAGAGCCATTAAACTTGCAACTGTTGAAACAAGAAGTGATGTTGACTC
CACCCTCTTCACCTCTAGAGCTTTTGACAACTGGGGGTTGTATACCAGAGGAATCTAAACCCAAGCGGGGTCGAAAATCCTGGCCTAGGAAAAGGACTGC
TACTCACACCTGTGAGTATGCAGGTTGCGGAAAGACCTATACCAAGAGTTCTCATTTAAAGGCTCACTTACGAACTCATACAGGTGAAAAGCCATACCAC
TGTGATTGGGAAGGATGTGGGTGGAAATTTGCTCGCTCAGATGAACTGACCCGGCACTACAGAAAACACACAGGTCACAGGCCTTTCCAGTGCCAGCGAT
GTGATCGAGCTTTCTCTAGGTCTGACCACCTTGCTCTGCACATGAAGAGGCACTTCTAAGTACTGCATGTCTTGTACTGCCATGAAATTCAGAGCTTGCG
CTATATTTGGGCAAAAGGAATAACACAAGCACTACAAGTTATATAGCTGTATTATGGGACCACTGATATGTAACTTAACATACCATCTATCTTCAGAACT
GGAAGAATGCAAACGTTAAAGTGGGATTTTGACTGGATCTTCAAGCATTCCGTGTTCATTTTTATAAAATGACTTGAATCATCAGACTACAACATTGTTA
TAAGTGACTGGAATGGACATTTTGGTCAACCATTCAAGCTTAGAGGGCAATGCTACAAGTTACCAAATAAGTTTATGTAACTTTTGAGGAATTAATGCAA
TCCTTCAAGAGAAAAATCACAACTACAAACTTGTATTTTTTCATTCCTAATAATTTCTAACTAATCACAAAAACAGGAAAATTTTCAGGTACAGAATGTT
GCTGGAAGGATTGAAATTAGTTAATGGTGCTTACAGACTGAAGCTTTTAATGGTACCAAAATGAAGCCAAAGTTCTCAAACTGCTGCATATCTGACAAGG
AAATCTATTTTTGTCTTCCGATATACAAGTATGACCAAAGTCAGGGATAGAAACCTGATTTATTTTGTTAATATATATATATGTATATGCAGACAGTATG
TGACGCACTGTGGTTTCTATGTGCAATAATTTGTACTATTTCCAAATATGCCTTAAGCAGAACAAAATGTTTTTCTATATTTGACTTTACATAAAAAATA
TGTAATATAAAATTAAGCAAACGTCTATTTTGTATATTTGTAAACTATTAAAAATTTACTGTCACAGTTTTTTAATTTACATATTCAGTAAGTTTTGAAT
AAACGTAGAAATATTTTATATGATGGAGGGAGACAGCTGTCCTTTACAAGGTCTTAACTTTCCTTTGCGATTTTTCTGTTTGCAGTTGTTACATTTTTTT
TTCTCTTAGGTTAGATGCCGACCTCATATAGATAATATCACATGGGCAGTGTTGTTTTTGTGATAGACAAAATGAAACAGCAGAGCAGTCTGAGGCAAAG
TATTTTAACAAGACTAGCATTTATTGCTCCCCAACCATAAAACTATAAGCTCCAACATCAAAAATGATTTCAGTAGAGACATCAATGACATGTTACTGTC
AACCAACAAGCAATCCAGCCCTACTTTGGACAGTGACTGGAGAAGTGCCTCTATTTTTACAGTATAATTTAAATACAGAATTACTTTTACAGTCAGTAGT
GACAAATTAACTCTATTTATAAAAGGAACCATTTTATAAAACCTTATTTTATCAATGTTACTCTTTGTCAGAACACTTGTGAATCATATACAACATACCA
TGTTTCTTTTATATTATTACAGGGAAGTTCCTTATCCTATACATAACTTGAGTGCAGCAGTATTGCATAGTGAACATTGACTATAGTCTACTTTTGTAAA
AGGGTATGTGTGACCGACAAAAGGTTAACAGGAATTTTGGCATGATAGTAATGATTTCATTGCTTGACCGAACTATCTAACTATATATGTAACTTGCTGC
AGTTACTGCTTATGTTGCATATTGTGTGATATGCCAGTTTTATGCTTTGTTTTATGAATACTGGAGATTGAAACAATCCATCAACAGGGCTTAAACACTT
AGCAAAGAATTTCCAGGATAGTCAGTTTTAATTCAAATAATGGAAGTGCCAAAAACAAATGAGTGCCTTCACCTGTACAAATGACGTTTAGAGATTAGTA
TACAAATTATGTCACAATTTTTTTTCCAGTGTTAACATTTGTATATTGGTTTCCATAAAATCACAGTACAATCATGAATCACAGTTTTTAACTGCACCAG
TTTATCCAACACTAACACAGCAATGATGTTTTAGAGCAAAATGTTCCAAACACTTTTCACAAAGGCCTCATATTTATCATAGGCAACAACCAGACCTTAC
CCATTTGAAATAGCACTGCCAATAATGCATTTAATTTTTGCAAATGCAATTAACATGTGATTAATTTTTATACATTATACTATCTTAGTCTGCCCTGTGA
TTTTTTTTTACCCACTGGAGCAGATATTACTGCCAAATGCAGAAAACATAGGATTGTGAGATAAGTAATGTATAATGGAACTGCTCTTGCCAAGATTGCT
TGAAATATAAAAATGTGGGATTTTTCAATGTACCTGCCAGCATGTTTCACTAAATGTTCATTTTGTGCAAGTGCATACAATTTAAAAAAAAAATGAGTTT
TAGTTACAAGCTTATGATCACAATATTGCTAAGCCACCACACCCACATTAATGCAGAGATGACCAATACTTATCGCAAGATAACAAGCTGCAAGGCAACA
ACCTATGAAAAGATGCAACCAGTGTTTAATAGGACCACCCTATGGGGTTTTGCTTAATATGGTATGGTGGCTTTGAAACATAATTATCATAAACTTGTAA
TGGACAACCCTTATATTTGAAAATGTATGCATTTGCTTGGGAAAAACAAAAGTTGTGAAACGGATTTATGTGGGTGAGACAAACATGTTATGGTGCAATT
TGTCCTTATAGGTGCATAGTTAGATAACCTAAAAGTTGGGGGTAAATACCAGCCAGAATAACTCACCGTGCTTATTTAAGCAATAGATGGCATGAGAAAC
ATCCATAAACTGTTATTGCCCATTGCTCTTTTTTCAAGAAACAAGTTTTTGCCATATAAACTGTTATTATGAATGTTATTTCCAGGGCCAACATTTAGGC
TCACTAGTAAAAGCCATTCTGCCACTTGTTATTGAATGGAAAGTTAATTGCTGTAACTGAATGCAAACTACACTCCATATGTTTAAGCTAAAACTTTCAT
CTGCTGTTGTTTTGATTTTACTGCCATTTATATTTGTTAAAATTGATTTGTAAAGCTGCTATATTTCAGAAACACATCCAATAAATATTGAAAGCCAAAG
ATGCAAAAAAAAAAAAAAA
5' EST: CX886587 [+]attctccagctctggtgactcgaagggaacctgaggaatttaatgagcttctggactttgattttatcttgtccaattcccttatccaccaagagtccat
gactgtgggttcttcttcttcatcttcgtcggtcactgcttcctcacctccacctcctgccacacttcctgatacaatgtctgctctctcgtctaccact
tgcaactttgtataccaggtacgttgtccccaggaatcatcagggggcacccttatgtacaatgcaagggatcctactatgtccagtgccactttcaacc
tggcggatataaatgatgtttcaccttcagggggttttgtggctgaactgatgagacctgacttggacccagtatttatgcaacaacagccacaagctag
cttgcaaggt
3' EST: CX886586 [+]tttttttttttttttgcatctttggctttcaatatttattggatgtgtttctgaaatatagcagctttacaaatcaattttaacaaatataaatggcagt
aaaatcaaaacaacagcagatgaaagttttagcttaaacatatggagtgtagtttgcattcagttacagcaattaactttccattcaataacaagtggca
gaatggcttttactagtgagcctaaatgttggccctggaaataacattcataataacagtttatatggcaaaaacttgtttcttgaaaaaagagcaatgg
gcaataacagtttatggatgtttctcatgccatctattgcttaaataagcacggtgagttattctggctggtatttacccccaacttttaggttatctaa
ctatgcacctataaggacaaattgcaccataacatgtttgtctcacccacataaatccgtttcacaacttttgtttttcccaagcaaatgcatacatttt
caaatataagggttgtccattacaagtttatgataaatatgtttcaaa
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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