XB-CLONE-3282517
Clone Name: IMAGE:7675441
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Gene:
nos1ap
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC125674 [+]
GACTGATGTAGCAGTCCGGGGATCGCTGCTAGCTCTGCCTGCCCTGTATGGGCTCCTGTTTGGGGGGAAGCTGCGCAACATGCCGGCCAAAAGCCGCTAC
AACCTGGTGGATGATGGGCACGACCTGCGGATCCCGCTGCACAATGAGGAGGCTTTCCAGCATGGCATCAGCTTCGAGGCCAAGTATATTGGAAGTTTGG
ATGTCCCACGGCCCAACAGCAGAGTCGAGATTGTGGCGGCTATGAGACAGATCCGGTATGAATTTAAAGCAAAGAACATCAAGAAGAAGAAAGTGAACAT
CCTGGTTTCAGTTGATGGTGTGAAAGTCATATTAAGGAAGAAGAAGAGAAAAAAAAATGACTTGGCCTGGGATGAAAGCAAGATGCTGGTTATGCAAGAT
CCTATATACAGGATATTCTATGTGTCTCATGACTCCCAGGATCTGAAGATATTTAGTTATATTGCAAGGGATGGGGCCAGTAACATCTTTCGCTGCAATG
TATTTAAGTCCAAAAAAAAGAGCCACGCCATGCGTGTTGTGCGCACCGTGGGGCAAGCCTTTGAGGTTTGTCATAAGCTGAGTCTGCAGCACACTGAGCA
GAATGGGGATGGCAGAGAGGATGCAGAGAGTGACAGAAGCACAGACTCTGAAGGGCAAGTGACGCAAACCGTAGCCAGTGTGGTTCAGGCTGCTGAGGAG
ACAGATATTGATGCACTTGATATTCCTTTGCCTGAGAGGGAGGGGCCAAGGATTAACCGTGGAGTGACCGATTTGGACGCTGTACATAAGGAGGATGACA
GCAGTTTTGAGAACAAGGAGCCAGTCAAACCTGCTTCCCCCAAGATGCTGCTTCCATCCAGCAGTGAGCTCCCCCCTGGGTCTCCCCTATCTGTTCATCA
TCAAATACAGCTTCTCCAACAGCAGCTTCAGCAGCAGCAACAGCAGACGCAGGTGGCAGTGGCTCAGGTCCATTTGCTGAAAGACCAGCTGGCTGCCGAG
GCTGCTGCAAGACTGGAATCTCAAGCCAGAGTCCATCAGTTGCTCCTGCAGAACAAAGACCTGCTGCAGCATATCTCCCTACTGGTCAGACAAGTGCAGG
AGCTGGAGTATAAGCTGTCTGGACACAATGCCATGGGATCTCAGGACAGTCTCCTAGAGATTACGTTCCGTTCCAGCATTCTGCCGGTGCTTTGTGACCC
TTCAACTCCGAAGCCAGAAGATGTTCCCCCACTGACTGATCCTGATTCCTCTTTTCAAGTTTGCAGTCCTTTAGTTGATCAAAGCATGTTCAAGAACTCC
AATGCAACTCAAGTGCCAAAGCTACAGCAGAGTCATTCTCTCACGCAACTAACACGACTTGCCGGCAGCACTAGCTCGGAACCTTCAGGGGTCAGTATTC
TCGCAGGCAGCACGCAGCACCTAAAGAACCTAGGTAAGGCAGTTGGAGCCAAGGTGAATGACTTCCTTCGGAGGAAAGATAATGAAAAAGGAGACATTGG
AGTAACGGAAGTCAACAAGACGGTGGCTGCAGAATGGTCCAGCAACGACAGAACATGCAGTGGGAACCTTGAAGAGGGACAAATGTCCAAGAATCTAGAA
AGTTTTCCTAGGCTTGATCCTCCTCCAGCGTCTGTGAAAAAGCGAATTCCTCGTGCTCTGAAAACCACCCAAGACATGATGATCTCACCCAACCCTATAC
TAAGCAGCCCAGAGAAGCCTTTGCCTGCCACTGCCGAAGTGCCTAAGGAGACATTCAGTGAAGCACGTGCTGAAAAGGAGAAACCACTACTGACCAACCA
CCAGCAAGAAACATTAATCAATGGAAAAGGAGATGGTGAGATTGTGGACCAGACAGAGAGTGTAAAGGGAAATCATTTAGAGGAAACGCTCTCAATACCT
GACCTCATACACAAGGAAAAGTCCGAAATAAGTCTCAAAGTCATTTCCTCCAACCATATAGAAACCATGAGCATCAAGAGCAGTATCAGCCAAGTTGAGC
TGCTGGAAGAGGAATATAATAATGTGGATTTGGGTTTATGGCCAAGAAACGCCACCTTAGATACAGACAGTCCACACCCAGACCTCCTCTCCTTTGACTA
GCGTTTAGGGTGGATGTTGGAACCACAATCCATTATTTGGTTGTACAGTACTTAGTTTAGTTACTGTATCTATGTGTACACTCATCAATGGTGAGTCGTA
ATAATGGCTGTTCCCATGATGCCTGCCTTACAGTTAACCTACAATATATAATACAAACACATTGGTATAAAGAATTGTGCAATCATCTCTTAAGTGCCTG
CATTACATACTTTGTAAATATAGTATATTCAGTTATGCAATTTCTTTATTCCACCAGGAGACTGGGGGCCCCTATAAAGCCTTCAGACCTCCAGCTGTGT
TAAATCTCCCATCATCCTTAGCTAGTGGGAGCTGTAATGGATCACAACTGGAAGACCTTAGTTTTAATATCCATGTGTTTATTAAGCATTTCAGGGTTTT
TAGGTAAAGCTGGTTTTTGAGAAACCTTTTCTGACATTTTTGCTTTTTTGGAAGCACGGGCCTCTCAATGAACCAGAATTTTTTATATGTGTATTAGTAT
GTTTGCTATCTGTTTAGTGCTGCGGAAGTGCTATAGAATGAAAGTGAAAAAAGGCATCTCATTTCAAATATACTGTAGGATGAAAATATTCATTGTTCAT
TTAGTTATTGTTCATTTAAATTACTCTTTTGTGTAGAAAGTTTCAGTGGGCCAGTGCCTTAATGTTCTAGCAAATACAAATGCCACTGTTAAGTATTGCT
AAGCACAAGTAGTAGGATTTGTCTTTCAAGGGTAGATATTCCTGCTGCTTCATGTTAAAGGTTTGTTTAATTCAGGATTCACAAAAGGGCTGTATTCATA
TTTAAACATATTTGTTATACATTTTTATATCTTTTATTTTCTATGAGAATTTCATAGGAATTTTAATGTGATGGTCAGTATGACTCCATGCAAAGAAATG
GTTTGAGCTGCGCAGGTTTCTGGTGCAGGTTGGCTACGATTGGCTGGCTGACTGAGTTAGTGTGTGTGTCTGAAACTGGCCATTACTTTGCACTGGTCAC
GTTTTAGCCTTCTTTGCTACTTCCATGGCTAGAATTTTGACCAATTCCCCTTACCGGCAAGTAGGTTGTTTCAGTGGATCAGCAATGTCACATCATAAAA
ATAAAGGCCATTAGGTCTGGACTGAGATTAAAAATCAGCCCTAGCATTTAAAGCACAATACACAGCACAATACACAGTGACTGTCTATGGCATTTTACAG
CAGCCCCTTTGGCATTTGGCAGAATACACAGATTGCCAGTACGGGTCTAAAGGCCATAAGGCATAATGATTTATGTAAAACAGGGGGTGCATATAACCTA
ATGTAAGTATGATGTATGTGTGGAAGTAAGAGGAAACCTTATATAAGGCAATAAGGCAAGGGTGGGACAGTAGGCAGCGTGCCTAGACCAAGTTAGTTGC
ACATGACATACCAAAAGAGGCCAGTGTACAGATAGCAAGCTGAAAACCTGTACAAATAAAGGAATATTAAACTGGATGTTGCAGATGATGCCATAATCTT
GCCTTCTCTATACTTACCCAAGCACCAGAATGTCACAGTTTTCCTGGAAGTGCCTTGCAGTTTATGCAAAGACATTCTCATCTACAGTCCTTTGCAAAGT
GCTCGCTTCTCATGATAAAGGCCAGTGTTCCTTTCAATTCCAGCACTATGCCTATGTGGCCTAATACTCTGTCTGGGGTATCTCTTAGCTTGACTCATGT
ACTCAGTCTGTGTTCGACTAATGCTGCTCATGACTGCCATCTGTGGGATACAAGCGGATTTGAAAACACTGCTCTGACCTTCTATGCACAAACTAGAATG
CTGTACAAAAGCTGAACAGCAAATTGGTTTTGACTAGGAAAAAGACAATGTGGAAAATCAACATTTCCCAGATCAAAAGTATGGAATGATGCTTGTTATT
AGACTGAGATGCATAGACTGCCACACCCCAACCTGCAGTATCTTTTCTACTATTGCCTCCCCATATTTTTCCAGATTTTTTATCACTGTCTGCATCATGC
TACCAGTTACTCTTAGGCTAAGAGTTTTTTCCACAATTTTAAAAGGGCACATGGCAGTGGAAAAGTGGCCAAATGTGCATTGTCAGGCAGTGGTAGAAGA
AATGGCAGCAAATTTGCTTAGCATTCTTTTTTTTTTTTTACTAAGTTGCCAAAAGCTGGAGCAGGTGACCAACATAATGCAGCCTTTATTAATGCGTGGG
AAATGTGAATAAAGGCCTAATATTTTGAGTGATTAGTGAATTACAGGAGAGGCAATATATACCTGGGTACAAATGTTGGACACATGAGAATTGGTCTTTT
TAGGCTTCGATGAAGCAAATATCAACAGGGCCCATTTAAAAAAAGGTACTCCGCTCCAAGAGGGTAAAGCATTGCTAAACTATTTAGGGACCCACACGAT
ACAATGGCTGCTTTATTTTAATCTTCATCCTGTATTGTGGTCCCAGACCTGTAAACATTCTGAGGCCTGAGACATCCAGTGAGGTTCTGTTAGAGAAAGG
CTGATACAAAACACTTCATATTGAAGAACTGAAGTCCAGGCTGGCTTTGCTGTTGTTACCTTTTGTCACTTTATGTTGCGGTGCCTTGGATAGTAACCTG
TCAGCTGGATAGGGCAGGCTGATGTCCTGGACTGGGTAATCACATTGGCAGGGCCATGTGAAAAGACTTGTTTTTGTATGGGAAAACTGTCAGGCATTGA
AGGTTAATATATATATATATGTAAAATTATATTTTGTTTCGTAAAGATACATACTGTAATGGCATTTATCTACTGATTCAGACCCATAAAATTTAGGAAG
ACTATATTTTCTATTTATATTTTATAGCAGAGTTGTACTGTACTTTTGTAATCTTTCGTACATAAACTGCACAATAAAACATCTATATAATTATAAAAAA
AAAAAAAAAA
5' EST: CX886967 [+]ctgatgtagcagtccggggatcgctgctagctctgcctgccctgtatgggctcctgtttggggggaagctgcgcaacatgccggccaaaagccgctacaa
cctggtggatgatgggcacgacctgcggatcccgctgcacaatgaggaggctttccagcatggcatcagcttcgaggccaagtatattggaagtttggat
gtcccacggcccaacagcagagtcgagattgtggcggctatgagacagatccggtatgaatttaaagcaaagaacatcaagaagaagaaagtgaacatcc
tggtttcagttgatggtgtgaaagtcatattaaggaagaagaagagaaaaaaaaatgacttggcctgggatgaaagcaagatgctggttatgcaagatcc
tatatacaggatattctatgtgtctcatgactcccaggatctgaagatatttagttatattgcaagggatggggccagtaacatctttcgctgcaatgta
tttaagtccaaaaaaaagagccacgccatgcgtgttgtgcgcaccgtggggcaagcctttgaggtttgtcataagctgagtctgcagcacactgagcaga
atggggatggcagagaggatgcagagagtgacagaagcacagactctgaagggcaagtgacgcaaaccgtagccagtgtggttcaggctgctgaggagac
agatattgatgcacttgatattcctttgcctgagagggaggggccaaggattaaccgtggagtgaccgatttggacgctgtacataaggaggatgacagc
agttttgagaacaaggagccagtc
3' EST: CX886966 [+]aattatatagatgttttattgtgcagtttatgtacgaaagattacaaaagtacagtacaactctgctataaaatataaatagaaaatatagtcttcctaa
attttatgggtctgaatcagtagataaatgccattacagtatgtatctttacgaaacaaaatataattttacatatatatatatattaaccttcaatgcc
tgacagttttcccatacaaaaacaagtcttttcacatggccctgccaatgtgattacccagtccaggacatcagcctgccctatccagctgacaggttac
tatccaaggcaccgcaacataaagtgacaaaaggtaacaacagcaaagccagcctggacttcagttcttcaatatgaagtgttttgtatcagcctttctc
taacagaacctcactggatgtctcaggcctcagaatgtttacaggtctgggaccacaatacaggatgaagattaaaataaagcagccattgtatcgtgtg
ggtccctaaatagtttagcaatgctttaccctcttggagcggagtacctttttttaaatgggccctgttgatatttgcttcatcgaagcctaaaaagacc
aattctcatgtgtccaacatttgtacccaggtatatattgcctctcctgtaattcactaatcactcaaaatattaggcctttattcacatttcccacgca
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: