XB-CLONE-3281805
Clone Name: IMAGE:7676107
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Gene:
stag1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC166175 [+]
gataccgccagatggcggcttaggattgctgctccctgccaggcgcggcccggggttagccacattattttttattattattttatatttattgcacccc
ctgtaataacccggacttaataataataatacaactataaattgggacgagccctgtggtgttgagtggagacagcggcccggggcctgggccggggtat
taaccccctgtcgccggatcctttgtgcacctcactgctcgcttcgttcttttctgaccccctcaggacggagctggacataaggaggaggctggggccg
ggatgtacgcaggagattcatcagtgagacttctctaacatttggatttacatttcaaaaccaaccatttgagaaaagactattgtggaacagcaaatat
gatcacctcagagctgtcagtccttcaggactctacaaatgaaactgcagtcgtgcacacagacatgagtgcagttagcactgatatgagtactgagctt
gttgaagatttagaggtgaaaggaaagaggaaaagaggccgtccaggaaggccaccgtctgcaatcaagaaacccagaaaaacacctgccgatcgcagcc
gtgccgaaacaggatcaagaggccgtggaagagcaaatgggcacccccagcaaaatggagaaggtgaccctgtcaccttgtttgaagtagtgaaaatggg
aaaaagtgccatgcagtctgtagttgatgactggattgaatcatataaacaggacagagacattgctcttctggacttgataaacttctttatccagtgc
tcaggatgcaaaggtactgtaagaattgaaatgttcaggaatatgcagaatgcagaaataatccgcaaaatgacagaagagtttgatgaggacagtggtg
attatcctctcactatgcctggtcctcactggaagaagtttcgctgcaacttctgtgagttcatcagtgttctgatccggcaatgccaatacagcatcat
ttatgacgagtacatgatggacacagtaatttcactgctgacaggtctgtcagactcgcaggtccgagccttcagacatacaagcacactggctgccatg
aagctgatgacggcgttggtaaatgtggctctgaatctgagcattcaccaggacaacacgcagcggcagtatgaagcagagaggaacaagatgattggca
agagagccaacgaaaggctggagctgctactgcagaaacggaaagagcttcaagaaaatcaagatgaaattgaaaatatgatgaattctattttcaaggg
aatatttgttcaccgatatagagatgcaattgctgaaatcagggcaatctgtattgaagaaataggggtctggatgaaaatgtacagtgatgctttcctg
aatgacagctatttaaagtacgttggctggacacttcatgaccggcaaggtgaagtgagattgaagtgtctaaaggccttgcagagtctgtataccaaca
gagagctgttccccaaattggaactcttcactaacaggtttaaagaccgaatagtttccatgactctggataaggagtacgatgtcgcagttgaagcaat
ccgcctcgtcacacttattctacatggaagtgaagaagctttgtcaaatgaagactgtgaaaatgtttaccaccttgtgtactcagcacaccggcctgta
gctgtagcagctggagaatttcttcataaaaagttattcagtagacatgatccgcaagctgaggaagccttggccaaaaggaggggaaggagcagtccaa
atggaaaccttgttaagatgttagtcctcttttttttagaaagtgagttacatgagcatgctgcttacttagtggatagtttgtgggaaagttctcagga
gcttcttaaagactgggaatgtatgacagaactgctggttgaggagcctatgcagggggaggaagtgatgtcggagcgtcaagagagtgcattgattgag
cttatggtctgcacaatcagacaggctgcagaagcacaccctcctgtgggcagaggaactggcaaaagggtattaactgctaaagaaagaaaaactcaac
tagacgataagaataagctgacagaacatttcattgttgcacttccagtactgctttctaagtattccgctgatgcagagaaagtggcaaacctcctaca
gatccctcagtattttgatctagagctgtacagcactggtagaatggaaaagcacctagacagcctgcttaagcaaattagatttgtggtagaaaagcac
atagagtctgatgtgttggaagcctgcagtaaaacctacagcattctatgtagcgaagagtacacaattcagaaccgtgtcgaaattgcacacagccaac
tgattgatgagcttgctgatcgattctcacatgctgtagaagaactgctacaggaggcagaggaagcagatgaagacgagatttacaatgttatggcctc
attgaaacgtttaacatgctttcacaatgctcatgatttgacgaagtgggaactttttgtaagctgttaccgattattaagggctggaattgagcatgaa
ggaatgatggagcagattgttgtgcaagctctccagtgctcacactactccattctctggcagctggtgaagatcacagagggcaacccttccaaagagg
aaatgttgacattgcgaaaaactgtaaaaactttcttagcagtgtgtcagcaatatctctcaagtgtaactacaactgtgaaagaacaggccttcatgct
gctctgtgatctccttatgatattcagccatcaattaacaacgggaggcagagagaatctggtgctgcttgtttttaatccagatgtgggactgcaatct
gagctgctaaattttgtcatggatcatgtatttattgaccaggatgatgaaaaccagagcatggagggagacgaggaggacgaagccaataaaattgagg
cattgcacaaaagaagaaacctgcttgctgctttctgcaaacttatcatctatgatattgtagatatgcatgctgctgcagatatcttcaagcactacat
gaagtattacaatgactatggggacattattaaagaaacactaagcaaaacaagacagatggacaaaatacaatgtgcaaagaccctcatccttagtttg
cagcagctattcaatgagctggtccaagagcaaggtcccaatcttgacaggacatctgcacatgtcagtggcattaaggagctggcccgaagatttgcac
tcacatttggtttggatcagattaaaacaagagaggcagtggccactttgcacaaagatggaatagagtttgcctttaaatatcaaaatcctaaagggca
ggagtacccccctctgaacttagccttcctggaggtgttaagtgagttttcctcgaagctcctgcggcaggacaagaagactgtacattcttacctggaa
aagttcctcacagatcaaatgatggagaggagggaagatgtttggcttcctctcatctcctacagaaactcactggttacaggcggagatgaggacagac
tgtctgtgaatagtggaggaagtaacagcaaaggttcttcagtaagaagtaagaaaggccgacctcccctacataagaaaagaggtatagaagaggaaag
tattgacaactcctgggtgaccaggaacgatacaatgcagactcctggagcactaacaacaccacagctaacatcaactgtgctgagggaaaatcctcgt
cagttaccagaacagatacctgagcaggagtcagaacacagctcagaaccagactttttgcataaccctcagattcaaatgtcctggttgggccaacaga
aattagaagatttaaatcgaaaggatagaacgtctatgagttacatgaaagttaggagtggtgtacgacatgcagtgcgtggtctaatggaggatgacgc
agaaccaatttttgaagatgttatgatgtcatcacgagggcagctagaagacatgaatgaggagtttgaggacacaatggttattgatcttcctccttca
agaaacaggcgggagagagcagagctccggccagatttcttcgattctgcagccatcattgaagatgattcaggatttggaatgccaatgttctgagttt
tggtacctaccatggaactctttgtcaaaaggcctgtatactgtgagcttctgcgagaaactatttctgtttttctttttgttcaaaccaaattatttcg
gtaatgatggatttcggcctgggaacagtcccgtgtcccgcaaggaattcagtaatgccatctttttggattagtattggtagatctaagctgcaactat
cagtttgcatttctacaactgctttgaaaggaagctaaattataataactaaaaaaaaccctggaaaaacaaagcctgttttctggacaatccatcattg
ttacacctatttctactgtaaaactaaacatttcaacagttcttggttttttttttttcccaccgaagttaacttttggttttacgttaaactcatgtta
aatatcaagcaggcatggaggatgctgcctatggccccactttatggaaaggaacaagaacagactttggaaatgcactggaataaaatattaaaagaca
atgctaaagaacggaagccattttgtgtcacccttcttaaaaaaaaaaaaaaaa
5' EST: CX888291 [+]taccgccagatggcggcttaggattgctgctccctgccaggcgcggcccggggttagccacattattttttattattattttatatttattgcaccccct
gtaataacccggacttaataataataatacaactataaattgggacgagccctgtggtgttgagtggagacagcggcccggggcctgggccggggtatta
accccctgtcgccggatcctttgtgcacctcactgctcgcttcgttcttttctgaccccctcaggacggagctggacataaggaggaggctggggccggg
atgtacgcaggagattcatcagtgagacttctctaacatttggatttacatttcaaaaccaaccatttgagaaaagactattgtggaacagcaaatatga
tcacctcagagctgtcagtccttcaggactctacaaatgaaactgcagtcgtgcacacagacatgagtgcagttagcactgatatgagtactgagcttgt
tgaagatttagaggtgaaaggaaagaggaaaagaggccgtccaggaaggccaccgtctgcaatcaagaaacccagaaaaacacctgccgatcgcagccgt
gccgaaacaggatcaagaggccgtggaagagcaaatgggcacccccagcaaaatggagaaggtgaccctgtcaccttgtttgaagtagtgaaaatgggaa
aaagtgccatgcagtctgtagttgatgactggattgaatcatataaacaggacagagacattgctcttctggacttgataaacttctttatccagtgctc
aggatgcaaaggtactggtaagattgaaatggtcaggaatatg
3' EST: CX888290 [+]ttttttttttttttttaagaagggtgacacaaaatggcttccgttctttagcattgtcttttaatattttattccagtgcatttccaaagtctgttcttg
ttcctttccataaagtggggccataggcagcatcctccatgcctgcttgatatttaacatgagtttaacgtaaaaccaaaagttaacttcggtgggaaaa
aaaaaaaaccaagaactgttgaaatgtttagttttacagtagaaataggtgtaacaatgatggattgtccagaaaacaggctttgtttttccagggtttt
ttttagttattataatttagcttcctttcaaagcagttgtagaaatgcaaactgatagttgcagcttagatctaccaatactaatccaaaaagatggcat
tactgaattccttgcgggacacgggactgttcccaggccgaaatccatcattaccgaaataatttggtttgaacaaaaagaaaaacagaaatagtttctc
gcagaagctcacagtatacaggccttttgacaaagagttccatggtaggtaccaaaactcagaacattggcattccaaatcctgaatcatcttcaatgat
ggctgcagaatcgaagaaatctggccggagctctgctctctcccgcctgtttcttgaaggaggaagatcaataaccattgtgtcctcaaactcctcattc
atgtcttctagctgccctcgtgatgacatcataacatcttcaaaaattggttctg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: