XB-CLONE-3281795
Clone Name: IMAGE:7676301
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Gene:
plekha6
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC128646 [+]
GGGTGCTTTTTCGCCTTGGGCAGTCACAATGATCATCTTGCCACAGTCCAGCATTCAGTGACTGTACTGATCTCAAGAAAGAGTGGACCATTGTGGTTTG
ACATGAGTTCTGAAACATCTGGAAAGCGCTGTACGAGTCAAGTCAACGAAAGCTCTGCATTCAGTATGGTTTCTGACTCCCCTTTAGAAGGCTCAATAAT
AAGGCCTTCACGTCTTTCAAAGAAAGGGATAGCATTTGGTAAACGTTCCAATTCAATGAAGAGGAATCCAAATGCTGAGGTAATGAAGACTGGCTGGCTC
TACAAGCAGGCTAGCTCTGGTGTAAAGCAGTGGAATAAACGCTGGTTTGTCCTTGTTGATCGTTGCCTCTTCTATTACAAAGATGAAAAAGAGGAAGTTA
TCTTGGGAAGCATTCCTCTTCTAAGTTTCTGTATTGCCCCTGTGCAGCCTTCTGATAACATCAGTCGTAAACATACCTTTAAGGTGACAGTATGCTGTAT
AGGGGGCGTCTCTCTCAATAACACAGCCCCTATCTCTCGGCAGGCAGAGCATGCTGGGATTCGAACTTATTTCTTTAGTGCAGAAAACAGAGAGGAGCAG
GAGTCGTGGATATGCGTGATGGGGGAAGCATCACAGGTTCAAAACCTGGCCACTCCTAGGATGGAAAAACAGGACAGTGAGAACATACCATCAACAAAAG
ATCGTGAACCTGAGACCAGAAGTCGTGGGGAGGGAGATGGGAGAGGTTCTGAAAAATTTGAGAATAGGTCAGAATGCAAGCAGGAGTTTCTGACCAAAGT
TAATGGGAGGGGGGTTCCTCCAGATAGACCCCCTGACCTGCAGCCACCCAGGCACTTTCCTGAAGGGGAACAACCACCACAGCCCAATGGATGGGGTTTC
CCCCCCTCTCCTGGCCGACCAGCAAGCACTGCCTTCTCAGAGGGCCAGAGGCGTGGTAACCTACCCCGAACTCACCCAGAAAAGGTAGCACAGAGGCAAA
ACTCTATGTCACAACTACAGCAGTGGGTTAACTTAAGGAGGGCAGCAGGTCCTACTGAGGAGATACAAAGCCCCAGCAGGTTCTTCTCAGAATCTCGCAG
AATACCTGAATGTTACTCCCAATGCCCTTCTCAGTATCTTGATGGCTACCAATATTACAGCACACCTGCAGGATCACGGCCAGACAGTATCTGCTCAATG
CCTATTTTTGACAGGGGAGTTCCGCAGTGGGGTGCAGAAGACAAAAGGCAGATCCACCATAAACTAGTTCCTTATTACCCACGGGACTGGAATGAAGCTG
GGAGCTATGGACGTCAGACTGAGATGTGGGTTCCCTGTCCACAACTGTATTACCAAGAAACAGATGCTGCATGTGAATCTCTTAGACATCTGTCATTACA
GCCTCGCTCACGCTCAGTACCCCGTTCTAATAGTCAAGGAACCTACAACTACACACGCCCAAGGGTTTACTCTCCTGTCCGTTCCCCCAGTGCCAGGTTT
GATGGCGTAACACGGGAAGATATTTTTGGAGACCCAGCAGCATATACCATGAGACGTTCAGTCAGTTCCCCAAAATATGGTTACATAGGAGACAGGCATC
CTCATCAAGCTGGAGTGTATTCATACAGCTACCCTGCATCCCCCCCTCTTCATGATAAAATGATGAGTGAGAGTGAGACTCTGATCAGCATGGTGAATAA
CATGGTACACTCTTCCCCCCGAGCACAGATCTTCATGCAAGTTTCTCCATACTCGGACCCATACAGAGAAGCACTGCAGAGCTACAAGATTAATGAACAC
GACACAGATAAGCTCCTTGGTCAGCTATGCGAGCAGCACAAGGCAGTGAGAGAACAGGACCGCCTGGTACAGCAACTTAGAGCAGAAAAGGAGAGTTTGG
AGAGTGCTCTGATGGGGACCCACCAGGAAATGGAGATGTTTGGCAACCAGACAGCATTTCCAGAAAATCTGCTTCATCGGAAGGAATCCCTTCAAAATCA
GCTCATCAGCATCCGCGTTGAATTGTCACAGGCCACAACGGCCTTAAATAACAGCAGCCTAGAATATGAGGCTTTAGAGGGAGAGGTCTCTGCTTTGCAT
GATACCCTGTGGGAACAGCTGAATCTCAATATACAGAATGAGCTTATGAACCGACAGATCCAGAGAGAGATTTGGAGGATTCAGGATGTCATGGAGGGAC
TGAGAAAAAATAATCCATCCCGGGGAACTGACACGGCAAAGCACCGCGTATTGCCTGGACCCTCAGCTACCTTCAGCTCCAACAGCCCTGCAAGTCCCAT
AAGCTCTGCTAGTCTTACAAGCCCCCTCAGTCCCTTTTCTCTCATCTCAGGATCACAAGGCTCCCCCACCAAGCAAACAACCAGTGAGTTGAAGATGAAT
TTAGAACCATATAAAAGAGAATCACTCCAGCTTTCATCCTCGCTTACTCCTGAAAGATTACGTTCCCTTCAGTTTTGTTCTGAGCATGAGTCAGAGAAAC
AACAACAAGTACCTCTACAGGTCATTAACAAAGTTGGCATTGTTCCCCCAATAACAAAATCACATAATGAAGATGAGTCAGAAATGAAACCATCAGTGGT
TAGACGAGAAGGTAGCAGAACACCTAATGGAAACATCTCACGAGAGCGGCCAAAGAGTGCATTATTCCCCAGTGAAGTAAAGTCCAAGATGAGTGTCGAA
GAGCAAAATGATAGGATGAAGCGTCACCAGACTGGATCAGTGAAAGAAAAGAGACGAAGCCTGCAGCTCTTAGCTAACCAGCAGTCAGAACACAGTGCAA
CCAAATCAACTGGAACATACAGAATAGTACGCAGATATCGCAGTGTCCACGAGGTTGATATATCAGACCTGGAAGCAGTTTTGCGTTCCCAGGAACCCCA
GCATACATATGAATCCCCTCAAGATGAGATTGCCCGGCTTCGCAAGATGGAGGTGGAGCCACAATCCTACAGTGTAGATATCACCAGAGAGCTTCCTGTC
CCAGACAAGGTGCCAGTCCCAGAATGTTAAGTTTTTTCTGACTCGTGTTACCATGAGTTTATATTGTATGTTTAGCTCTGTATTCCATTTAGGCTGTTTA
TTATATGTTGAATATTTCTGTTTTCACTACTATGTACAGTACAAACACTATTTGTATCACACAGCGCATGCCTCCTGTAGATACCATTATGGCTCATAGT
GTATACCATACCCTGAATGTTTCACATAGTGTCACACACTGCACACCTGTCTCTGTGTCTCAGCTTTCAACCCCAGAGAAAGTGCTGATCCCAGAGCGTT
ACATAGAATTGGAACCAGAAGGCCCACTGAGCCCAGAGGAAATGCAGGAAAAAAGGAGGAAGGTGGAAAGAATCAAGACGCTGATTGCAAAATCCAGCTT
ACAGAACGTGATCCCACTAAGTGACTCGGAACTAGAAGTGACCCCTGATCCTGAGTCCCAGCTTCAGGAGCAAGAGAAACGAATAGAGTTATCTTGCACG
CTGGCTGCAGAAGCTTCACGTCGTGGGCGTCTCCTCTCTGCTCTTTGTGCCACCCCAAGTCCCCCTACATCACCCAAGTCCCCAGCTCCTCAGACCCTCT
CCTCCTCTCCTGAGCATTCGGCGTTGGGGGGAGGCAACAGCAGCAGGAGCAGTGACAGCGCTGGGGAATACGGGGCGAACATCATGTGTGTCTGAGCCAC
AGTCACAGGCCAGAAATCTTGCAAAACTACACAAAGACTAGTTCCACATACACACTCCAGCTGTTCTGACAATGCCAGAAGATTTCACCTCTCCTACCAA
ATACAGACATCACCATTTAAAGAGAAAAGAAGAAAGATTCTCAACGGATGAAGATTAGCGCATCATGGTGGAAGCCAGACTGTCCTAGTCTGATATATAT
TTAAAGCACTGATTTTGCAACCAAGTGGAGAGAAACTGTAGAATGATATTAGTTGGCAATTATTAAAATATTGCATGACCAAAACTAAGGAATTTCCCCA
GGGCTGTAAAGCCAGAGCCATTGAAACTACTTTGTACCAGACAATCATTTCTGTTAAGGTAGTCTATGGTCTTTTTTAAAGTGACTTTCACTAGAGAGTT
GTCACAGTATTGCTTAACATTTAGAAACACATATTTTCTGGACTTTAAATTACTTTTAAACTAAGCCACCAAAGCATTTTCTTGCACATGCATTATTTGT
CATATACAGTGCTTGAATTAGCCTGTTTGCCAATTTCTTTCTCCCCCATGGGTTTCTTCTTCTTGTCAACTGTTGCAGACTTCTGGGGGAGGAATTAATC
CTCCAGCAGCTCAGAGCACTCCAATGGCCCACCTTAACCCCCCCCCATAAAAAAAAAATTACAAAGTAGAATAAAAATGAAAATTGTGGTTCAGATAACA
TTAATGCCCCTCAATGTTCTTTTAGTTTAATATGGTAAATCTATGCATGCTAAGCATCAAAACTGTTCAGTAGATGGAAAAAAACAAAATGTCTTTGCTC
TAAGTAAAAGAAACCCGAAAGTCTGGGCAGATAAAAGCCCAGGGCTGACATTCTGACAGATGTGTAGCATTGTTCTATCTTTACAAAAACAAACCCAACC
TAATTCAAGCATATTTCTTACTATAAAAACATATATACATATATATATATATATATATATATAATGTATAGGATCAATTAATTATAATGCTTGGAGCCTG
GGGTTTTCTTGATAAAAAGATCTTTGGATCTCCATATCTGCTAAATCTGCTTAAAATAATTAAAACTTTTGTTTTGCCACAAATATAAATACATGCAGCT
TAGGATCAAGTACTATGTACTGTTTAAAGGAAAATTGTTTTAAAAAAACTTTGCTTGTAATGTTATAGACTTTATAGGAGATGGTCTTCCTGTTATTTGG
AGCATTCTGGGTAATGGGTTTCCAGATAGGGAATCCCAAACTTGTAATACAATATATATATATATATATATATAAATCTTTATATTTTACTTCATAAAGA
GAAAAATATTATTGTATGTATATAACTAGGGAAAGTGCTATTCATAAAGGTGAATGTACTATCCTCTTAAAGGGATAGTTCAATTTTTCAGTTACATTTA
GTATGTTATAGAATGTCATGTTCTTAGATTTGTAATTTGTCTTCTTTTTATAGTTCATTCATAACTATTTGCCATCTTTCTGTGCCTCTTTTCAGTGGTG
GAGGGGCTTCAGTTTAATTGTTTATCGTGTATTTTTTTATACTTCTCTTTCTTTCCAGACCCTCTTCTAGTCATTCATTACAAAATGATGGGGAAAAGTC
AGTGACCAATTTATTGCATGGGGCCCCAAGATAACTGATGGTGATCCAACATGCATGTATCAGTCCATATAATAGGTATACTCCTATCAAGAAAAGGGTT
ACAATTTTCTTTTATATTTTTCTTTGCAGAGTCATATACTATTATAATTACAGAGTTAATTTGATTTTACAATAAAAAAAAAAGACAACAGTCTATCAAG
TTCAACATATATGGAATGATCCCCAACCAGCATGATTTTAAGAGTGACAAGCCTCTCATCCACATCTGTATTTTGGTTACTTGCTTGTTCGTTCTGCCCC
AGGTGCACTTAATGTGCAGACAATTTTCACGTTTAAATCTCATGCATCATTGCAGGGTGGCAGCCTAGGGGCACCCACACTGGAAATTATATCCAGATTG
TTGCCTTGGATACTGTTGTTTGTCCAAGAAATTATCCAAGCAACTCTGAAAGACATTGATGGAATCAGCCATCACAACATCACCCAGCAGTGCATTTCAC
AAAAAAAAAAAAAAA
5' EST: CX888313 [+]ggtgctttttcgccttgggcagtcacaatgatcatcttgccacagtccagcattcagtgactgtactgatctcaagaaagagtggaccattgtggtttga
catgagttctgaaacatctggaaagcgctgtacgagtcaagtcaacgaaagctctgcattcagtatggtttctgactcccctttagaaggctcaataata
aggccttcacgtctttcaaagaaagggatagcatttggtaaacgttccaattcaatgaagaggaatccaaatgctgaggtaatgaagactggctggctct
acaagcaggctagctctggtgtaaagcagtggaataaacgctggtttgtccttgttgatcgttgcctcttctattacaaagatgaaaaagaggaagttat
cttgggaagcattcctcttctaagtttctgtattgcccctgtgcagccttctgataacatcagtcgtaaacatacctttaaggtgacagtatgctgtata
gggggcgtctctctcaataacacagcccctatctctcggcaggcagagcatgctgggattcgaacttatttctttagtgcagaaaacagagaggagcagg
agtcgtggatatgcgtgatgggggaagcatcacaggttcaaaacctggccactcctaggatggaaaaacaggacagtgagaacataccatcaacaaaaga
tcgtgaacctgagaccagaagtcgtggggagggagatgggagaggttctgaaaaatttgagaataggtcagaatgcaagca
3' EST: CX888312 [+]tttttttttttttttgtgaaatgcactgctgggtgatgttgtgatggctgattccatcaatgtctttcagagttgcttggataatttcttggacaaacaa
cagtatccaaggcaacaatctggatataatttccagtgtgggtgcccctaggctgccaccctgcaatgatgcatgagatttaaacgtgaaaattgtctgc
acattaagtgcacctggggcagaacgaacaagcaagtaaccaaaatacagatgtggatgagaggcttgtcactcttaaaatcatgctggttggggatcat
tccatatatgttgaacttgatagactgttgtcttttttttttattgtaaaatcaaattaactctgtaattataatagtatatgactctgcaaagaaaaat
ataaaagaaaattgtaacccttttcttgataggagtatacctattatatggactgatacatgcatgttggatcaccatcagttatcttggggccccatgc
aataaattggtcactgacttttccccatcattttgtaatgaatgactagaagagggtctggaaagaaagagaagtataaaaaaatacacgataaacaatt
aaactgaagcccctccaccactgaaaagaggcacagaaagatggcaaatagttatgaatgaactataaaaagaagacaaattacaaatctaagaacatga
cattctataacatactaaatgtaactgaaaaattgaactatccctttaagaggatagtacattcacctttatgaatagcactttccctagntatatacat
acaataatatttttctctttatgaagtaaaatataaagatttatatatatatatatatatata
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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