XB-CLONE-3281404
Clone Name: IMAGE:7676451
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Gene:
proser2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC127303 [+]
CTGATTTCTAAATGAACATTCCTACAGTCCTTCCCTCTGCTCCCCATACCTTCTAGTTACTCTGAACTCTACAAGTCGCAGTGAGACACCTGGCACATTC
ACCTGTGCGATTCCTCTTCAGCGACTGTGGGAAGCGATACTGAAGCGCACTCGCAGCACTGATCTCAGACCGGTCCCTGACCCCTTAGCCCAGGCTATGA
TTTATGCTTGTAAAAAATTTACAAGCCAGCCTTCTCTTCTTGAGGACTATTTACAGAAGCTCATTTTTTGAACAATGCCAAGCAACCTAATAAAACTGAG
CTCCTCAGAAATGGAACCTGACTTCCACAGCCGCACGTTTAGTGACTCTGAAAGAAGCGGAAGTTTCAATAGTCAGGGAAGTCGGCATTCCAGATCTCGA
AGTTCTAACTTGGATGATGAGAACCTCAAATATTTAACCAGTGAAGAGAAGAATGCCCTCCTGTTTTTTGAGGAAACCTTGGATGCCTTTGAGGATGATA
TAGAAGAGCCACCAGGTTCCCAGGATAATCATTTTGGATACTATTCAATATCAACAGAAGGAAGTCACTCAGAAAATGAGGATATCATAGACCTAGTCCA
GGCTGAAGAGAATCTAGAACTGCACACTCTGCCAAATTCACAAAGTATAGAGGAAGCTAAGGATGTAAAACAGACACAAATGAACACAAGTGTTCCAATG
GCTCTAATCCATACAGCTTCACCTGTAACTATCCCACCAATGGCCTCTCCAAAGGGAATATCTGAATTTCCTGAATTCCCCATAGAGCACAAAAAACTTC
TTGGTGCAATCCCAACTCCTGTTGTAATAGCTAAAAAGATTTCAGAGAAGAATTCAGACAATTTAAGTGCCTCGCCTTTACCACCAAGTGAGGGGAAACC
AACTGAATTAAAAAGGAGTGTTGCAACATCACCTATTGGTGAAGGACATTTCATGTTTACTGGCCCACCCAACTCTAAACATAATAATTTCCCAAACAAC
ATAAGTATCAAACAAGTTGGAAAACAGTATAATAAAACCATTGCCAAGGCAGCTGTCAATGTACAAGAGCGAAAGGCTAAAGTATTGGCCAACATTAGTG
GACCATCTTCTTTTGATGATGAAACAGATCGAGTCAATTATGATCGGCTAAGCAGGAAGACCTCATTTCGGGACGTGACTACGGAACAAGCTAGATATGA
AGCACTGACCAAATTAGGCCTAGTTAAAGAAACAACAGTGCAGGCTGGTATACCTACTTCTCCAGTCTCAAATGGTCCCATCTCTCCTAAATTCTTACTA
CAAGACTCTCACAAGAGATATTCACATGAATCACCGTATATTAGCAATAGCATCAAGAATGAGCCTATTCCCTTTGCACATAGTAAAATCTTACCACATG
ATTCTCCCAATAGATATCTTAATGAAAATCAAAATATCAGTAGTGTAAAGAGTGAACCTAGTCCCCTTGAAACTTCCAAAAGCTTACAACATGAGTATAC
AAGATTATCCATTGAAAAACAAAATACCAGCAATATGTTAAAAACTGAGCCTAGTCTCGCTACATCACCCAAGAGCTTACCATATGAATATTCAAGGAGT
GAGAATCAAAATGTCTTCAGTAGTATAAAAAGTGGGCCTATATCTCCCAGGAATGTAGCTCATGACATTCCCAGAACCTATTCAAGTGAAAACCAAAATA
TTAGTAGCTTTAAAAGTGGGCCTACATCTCCCAGGAATGTAGCCCATGACATGCACAGAACCTATTTAAATGAAGCCCAAAATAGCAGTAGCATTAAAAG
TGGGCCTGTAACTCCCAGGAATGTAGCCCATGACATTCCCAGAACATATTCAAATGAAAACCAAAATAGCAGTAGCTATAAAAGTGGCCCTACGTCTCCC
AGGAATGTAGCCCATGACATGCACAGAACCTATTTAAATGAAGCCCAAAATAACAGTAGCTTAAAAAGTGAGTCTAATCCCTATATAACTACCAAGAGTT
TACCGCAAGAAGCTCCCAGAAGATATTCAAATGACAATCAAAATAGCAGTAGCATAAAAAGTGAGCCTAATACCTATGTACCTCAAAAGAGTTTACCACA
TGAAGCTCCCAGAAGATTTTCAAATGAAAATCAAAATATCATAAATAGTGGAAGAAAAGATTCTAGTTACTCTATACCCCCAAAGAGTTTCCCTCCAGAA
TCTAACAGTATCAGTTATATTTTAAAGAGTGAGCCAAGTCCCTTTGTACCCATAGGGAAAACTGTTATTTTTAAAGGTGAAGCATTATCTCCTGAAAAGA
ACATCCAACCTATTGCTCCCCAGGACAATAATGAGAAGAAAAATTCAATTCTTCATCAGGACATTCGAAGATCTTACTCTACGCCAAGACCTACAGGGTT
CCGGCCTCAGGGTATTACAGTGCAGTTCTCAGGGCGTGATGCATCTGAGGAAACACGAAAGGATGCTCTACGTAAACTTGGACTCTTGAAAGAGAAGTCA
TTCTGAAACTGTGATTCTGATTTTCAACATGGCAGGATTTAGCAGGCACTGTCCAGAAAATCAGTGGCTTTGGTTTGAGTGTCTGGTAGAATTTATATAT
TATTATGTTTCACTTCTTCTCATTGTTTTTACTGAAATGTAAGGGACAGTGTAGAATGTGTAGAACAGAAATATGTCTGCTCTTCAATTCCAAAGATGAG
TTTCAGAATCAAAGGCATTGATTGTACATTTAGCACAGTATCGGGTCATAAGGCCACAACTATATTTTTGTATTTGTCTGTTTTTTCTTAATGAATACAA
AAATATGACATATGTTTGCAAGATGCACTGCATCAAATATAAATGTGTATATTGGGGGCTTTCCATCTCCCTTACATCTAAATCAAAGCGTAAATCAATA
CAATATCAATACAAATCAATACAATTTTACAGAGGGTGCCATTAGAAGGGGCAACTATATGTATTTCAAAGAATAATGCTGTGATTATAAAATGTTTATA
CAATGTATATGTATGTTTATTTGTAGCCAACTTGTACATAAAATATTTTTATGCATATTTTTCTTTTTGTAAGGACAATGTAACCCATGAGACCCTTTCC
AACAACTGCAGATTTCTATTTGCACTGCACTTATATTGGTGTTGATTAAGATTTAAAATATTCATAAAAAAATCTGAGATGTTATTTGTGTTGGGTAATA
CTGCCCAGAAATGCACAGGTTTGTTCTTGCTGTCAGGCTGCACTTTTAAGAGTATGTCGGTTTAAAGGAAAGCGTCTTGGGTTTCATGTTTTGATGATTG
AGATGCTACTGATACATGACAATGCAGATACTGGAATGTTTTGCTATGCTACTGACTACAGTACATTTAAGCAACTGCTACAGGGCTGTTCTGTAAGATT
TGCCATTAATATTACAAAGCCAACTATGAGATGACAGATTCCTGTCAGTTTCTAGGGCTTGGCAACATAAAGGGTAAGCAGTGTTTGCCAATATAGACTA
AAAAACGCAAGCATGCTGACATAAAACCATAGATTTCTTTACCAAATGATGTTTTGTTTTGCACTGCAATCATATTTAATACATAATGCATCAAACCTAA
TAGTCACTCAGTCTACCTCTGATTTTCAGTAAAACTGTTTTATAATATAATATACAAATCTGACCTGTGTGATATTTAAGGAAAATCAACTTACCTAGAG
CTGAATGTCCTTTTAAAGAAGATTTAGCAAAGAGCAAGTTTCTTCACAAACTAAGAGCCAAACACAGACTGCGATACTTGCACTACTTTACAGTGACAAA
ACCAGAAGTTCAGAGTCAACAGTAGTTAGCCAATGCTCAGTCTACCTCAAATCCACCTTTCTTTGCTTAAAATTGTCATTCATTTGGAAATCTTTTTATT
AGGATTAAGATATAAATACTGCTTGATGCAAATTAAATGGACTCTAATTTATCAGTATATTCTTCTATAGTGGGCTACTAGTCGATCTGAGAACCTTAGG
TATAAAACCCTTGTATTCAACATGTATGCAATTTTCCAATTACTGTACTGCAGAAATTTTTCTAGAAACACTGAGATGCTGGAAAGCTTACCCTTTCCCT
TTGGGTACATTTTCTACATGCACCATTTGTCTGGGTGCCTTGAACCATAACAGTGTGTGAAAATTGATAAACATATAATCCTTAAAATTATAATTTTTGA
GAGCTGTGTGACCATCAACCAGGGACAAAGACCTGTTATTTTGGGGTTGGCTTTTTTTTTTTTTTTTTTAGTATCTACAGTATATACATTTGTTCATAAG
TAATCTTATAACTTCACAGCAAATAAACTGGCCTTCAGGCTGGGTCCCTATCTAACCGAACTGCCATTCCACAGTCTTATAACTTAGGATGTTATTAATT
TTACACGTCTAAAACAGAATAATGGGTATGGGGGCAGAACCTAATTCTTAACATAATATTCGAAATGAGGGATCACCCGGGAAACCAACCCCAAGAATTA
AACTGAAATGTCCAAGATGATTACAATTTGTATTTGTGATGTCATTGTTTATGTCATACTGTCTGTTACAATAACTATTTATGTTCATATTAATAAATAC
CATGTATCATATCATGTTCCGTGTTTAACTGCTGTTGCATTTAATAAACCATGTTAAATAATGACAAAAAAAAAAAAAAA
5' EST: CX889030 [+]attcctacagtccttccctctgctccccataccttccagttactctgaactctacaagtcgcagtgagacacctggcacattcacctgtgcgattcctct
tcagcgactgtgggaagcgatactgaagcgcactcgcagcactgatctcagaccggtccctgaccccttagcccaggctatgatttatgcttgtaaaaaa
tttacaagccagccttctcttcttgaggactatttacagaagctcattttttgaacaatgccaagcaacctaataaaactgagctcctcaganatggaac
ctgacttccacagccgcacgtttagtgactctganagaagcggaagtttcaatagtcagggaagtcggcattccagatctcgaagttctaacttggatga
tgagaacctcaaatatttaaccagtgaagagaagaatgccctcctgttttttgaggaaaccttggatgcctttgaggatgatatagaagagccaccaggt
tcccaggataatcattttggatactattcaatatcaacagaaggaagtcactcagaaaatgaggatatcatagacctagtccaggctgaagagaatctag
aactgcacactctgccanattcacaaagtatagaggaagc
3' EST: CX889029 [+]ttttttttcttttttgtcattatttaacatggtttattaaatgcaacagccgttaaacacggaacatgatatgatacatggtatttattaatatgaacat
aaatagttattgtaacagacagtatgacataaacaatgacatcacaaatacaaattgtaatcatcttggacatttcagtttaattcttggggttggtttc
ccgggtgatccctcatttcgaatattatgttaagaattaggttctgcccccatacccattattctgttttagacgtgtaaaattaataacatcctaagtt
ataagactgtggaatggcagttcggttagatagggacccagcctgaaggccagtttatttgctgtgaagttataagattacttatgaaacaatgtatata
ctgtagatactaaaaaaaaaaaaaaaaaagccaaccccaaaataacaggtctttgtccctggttgatggtcacacagctctcaaaaattataattttaag
gattatatgtttatcaattttcacacactgttatggttcaaggcacccagacaaatggtgcatgtagaaaatgtacccaaagggaaagggtaagctttcc
agcatctcagtgtttctagaaaaatttctgcagtacagtaattggaaaattgcatacatgttgaatacaagggttttatacctaaggttctc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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