XB-CLONE-3280123
Clone Name: IMAGE:7677844
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Gene:
nme5
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC123011 [+]
AGCTTACTCTCAGGGGAAGCGGCTGAATTGGACTGGGACTCGTTGTTCCGAATCAGTAACATTTGAGATGACTTTAGAACAAAATCAGGCGATGGGACAG
TCTATGAAAGCGCCTAAGATTTATGTGGAAAGAACTCTCGCTATAATAAAACCAGATGTCCTTCATAAAGCAGAAGAAATTGAGGATATCATACTCCGGT
GTGGGTTCCACATTGTTCAGAAGAGAAAAGTGCATCTCAGCCCTGAGCAATGCAGCGACTTCTACTCAGACCAGTATGGAAAGATGTTTTTTCCAAGTTT
GACTGCCTATATGAGCTCAGGCCCAATAATCGCAATGACATTGGCAAGATATAATGCCATCTCCTACTGGAAGGAATTAATCGGACCAACAAACAGCTTG
AAAGCAAAGGAGACACATCCTGAGAGTTTAAGGGCAATATATGGGACTGATGACCTCCGGAATGCCCTTCATGGAAGTTACTGCTTTACATCTGCTGAGA
GAGAAATTCGATTTATGTTTCCTGAAGCTCTTATTGAACCTGTGCCTATTGGGCAAGCCGCAAACACTTATTTAAATTTATTTGTCAATTCAACATTGAT
TGCTGGGCTCACAGAACTTTGCAAACAAAAACCAACTGATCCATATATATGGCTTGCTGATTGGCTTCTCAAGCATAATCCCAACAAACCAGCTGTGGAA
GACTGTTATTTTGTTGAAGAACCTACTGCATCATAATTGTTTGGCATTAAGCTTTAAAGCTTAACAGTCAGTGTTGCAAGGAGCAATGGATCTCTCTGTA
ATACAGCCAACGTTTGTTACATTTTTAATGTATTTTTCTGCATAAGTTGTTGAAAAACAATATGTAACTGTAATTGGGTGTGGTTTGACCTTTATATGCA
TAATAAGAGATTTTCATTAAATGTACTAAATAAAATGTTTAAGTGCTGCAAAAAAAAAAAAAAAA
5' EST: CX891385 [+]ctcaggggaagcggctgaattggactgggactcgttgttccgaatcagtaacatttgagatgactttagaacaaaatcaggcgatgggacagtctatgaa
agcgcctaagatttatgtggaaagaactctcgctataataaaaccagatgtccttcataaagcagaagaaattgaggatatcatactccggtgtgggttc
cacattgttcagaagagaaaagtgcatctcagccctgagcaatgcagcgacttctactcagaccagtatggaaagatgttttttccaagtttgactgcct
atatgagctcaggcccaataatcgcaatgacattggcaagatataatgccatctcctactggaaggaattaatcggaccaacaaacagcttgaaagcaaa
ggagacacatcctgagagtttaagggcaatatatgggactgatgacctccggaatgcccttcatggaagttactgctttacatctgctgagagagaaatt
cgatttatgtttcctgaagctcttattgaacctgtgcctattgggcaagccgcaaacacttatttaaatttatttgtcaattcaacattgattgctgggc
tcacagaactttgcaaacaaaaaccaactgatccatatatatggcttgctgattggcttctcaagcataatcccaacaaaccagctgtggaagactgtta
ttttgttgaagaacctactgcatcataattgtttggcattaagctttaaagcttaacagtcagtgttgcaaggagcaatggatctctctgtaatacagcc
aacgttttgtacatttttaatgtatttttctgcataagttgttgaaaaacatatgtaactgtaattt
3' EST: CX891384 [+]ttttttttttttttttgcagcacttaaacattttatttagtacatttaatgaaaatctcttattatgcatataaaggtcaaaccacacccaattacagtt
acatattgtttttcaacaacttatgcagaaaaatacattaaaaatgtaacaaacgttggctgtattacagagagatccattgctccttgcaacactgact
gttaagctttaaagcttaatgccaaacaattatgatgcagtaggttcttcaacaaaataacagtcttccacagctggtttgttgggattatgcttgagaa
gccaatcagcaagccatatatatggatcagttggtttttgtttgcaaagttctgtgagcccagcaatcaatgttgaattgacaaataaatttaaataagt
gtttgcggcttgcccaataggcacaggttcaataagagcttcaggaaacataaatcgaatttctctctcagcagatgtaaagcagtaacttccatgaagg
gcattccggaggtcatcagtcccatatattgcccttaaactctcaggatgtgtctcctttgctttcaagctgtttgttggtccgattaattccttccagt
aggagatggcattatatcttgccaatgtcattgcgattattgggcctgagctcatataggcagtcaaacttggaaaaaacatctttccatactggtctga
gtagaagtcgctgcattgctcagggctgagatgcacttttctcttctgaaacatgtggaacccacaccggagtatgatatcctca
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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