XB-CLONE-3280053
Clone Name: IMAGE:7677766
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Gene:
prpf39.2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC127269 [+]
GGGCAAGATGGCGACAGACTGGAGGTTGCCGAAAGCTGTATGTGCAGAAGCTGAAGTTCCCTTTACCACTGAGGAGAGTGATGATGAGGGCGAACCAAGT
GATCCAAAAGAGCCAGAACTGCCGGCTGAGTTTAAAACACTTTGGGAGGAAGCCACAGAGAACCCATACAACTTCAACGGCTGGGCAAAAGTGTTGGAGT
ATGTTGAAACAACGAATAACCTTGTGGCAGGCAGAAAGGTCTATGATGCTTTTCTCACACGCTTTCCATATTGTTATGGCTACTGGAAGAAGTATGCTGA
CCTAGAACTTCAGTTACGTAATACAGCTGAGACAGAAGAGGTCTACTGCCGGGCACTGCAGTCTATTCCGCTTAGTGTGGATTTGTGGATAAATTACATA
ACATTTCTTAAAAACACCCTGGATACAGCACTGCCAGAATCCATTGAGAAACTGCAAGGGTGAGAGAGTAACTCTCTGTCTGTCTGTGATTGTGAGGTTA
TAAGAATGTAATTTGTGTGAACTGGAGGCAACCATCATTACGTCAGGAAAGAAAGTCTTAATTTTGTTTCCTGTTATAAAAACAAGTTGGGTTTCATTAT
TTCTAGAGGTGTGTAGTGTTTTTATAAGTGAATACCACAGTCTGTGCAGATCATATGTTTTGATCTTTATAGTATTAAACTGAAATTAAAAGGGGTAGTT
AACCTTTAAAAGGCAAGGAAAGGGTTACTAAAGGGAGATGCCAATTTGTTAGCCACCTCCAGTGATTTTAATTGCTAACATCAGATCACAGGTACTGTCC
TGTTGAGCACCATGCTACCATGTTGCCTCTTGCCCTGTGACCCCTTACAGCCCCCCTGGGCTGAGAGCATGTGCAGCAGGGTAAAATCGGGACTTTTACA
CTTCTATGCATGCAAGCCAATATGGGCAAGAGCAAGAGATCCCTGGGCAAGATGGCAGAATGTCTGGCAAATATCTCAGAAACCACTGAGAAAAAAGAAA
TAATGTAAATTGCTCCCTGAGCAAATTTACTCGCTTCTTGTTTAAATTCTGTTTCCTATTAAGGTTTAAAGCTACAACACACTGAAATTGCAAATTACCT
TCTTTAACCATCTTGTCTTTCTAAACCATATTAGAAGCTGTGCATTTACATTAATGTTATATATTTTTCATATGTGGATTCCTGTAACACAGCAATACCA
CTGGTTGTAGTTTTGTTTTCATAAGCGGTTGATTTTGACTGTGTGAGTTGATAGTCCAGATTGACTGCATTGTGTGGGTTTTTCTCATGTAAAGAGCAAA
GGCGAAATGACTGGTTGGCACAATTTGTGTGTATTTTGATATGCATGCTTGATAATACAAAGTATTTGCATATACACCTGTAACCAAAGCACTGAACTGG
GGGGCTGGGCTTTAATTTGTGGCGGCCTTTGGTTCACCAGAATTCACTGGTTTGCTAGGAGTACCAGGGAAACACTTCAAAAATGAATAAAAAATACCTT
TTGCTAGACAGAATAAACTTATAAAGAGGCAATGACTTTTTCTCACCTTAGTACTTGGCTAATTAAATATTAATAGGCAAATTATGATAAACTGGGGCTT
TCCCTAGCATTTGTAAATGTTTTACTTTTATGTTTGACCTGCATGTGCCTTCTGCCTCAATACCTTAATAGTAATTTAATTTAGATTAGAAATCAAAATA
CATACTGCAACTGAACAAGGTTTTTTAGAAGGTTTACAGCTTTAAGCTCTAACCTTCCTCCTTTGCTTTGTGGTCCGACATAGGCACATTGTGTTCCTAT
CGAATTAAAGAACATAAAATATTGGCATAACAGTTACTGCTATAGAGTTTCCAAATCTCTGTGCCTCACAGTTTGGGAGCCTGTGGTGCAAATATAACCA
GTGAGGAAAAACTCTTTCCTAAAACCAAAGTGGCAATCGTGTTGCAGTAAATTCCAACAGATTAAAAAAAAAAGTAAAATTATTTATATATATATATATA
TATATATATATATATATAATTAATTTCTGTTTCAGTTATTAGATCATTTTTTACCTCACCAGTGATTTGCTCAGTCACTATCTTTTTGTTGTTCTTACAA
TACTTAATCACCATGCAAATATCTGATATAAATCTTTTAGATGAAAACATTGTAGGGATGTGTCCATGTCTAAAACTTTGAAACTGGAGCAAAATATCGG
GCGTTACTCTATAAGTATAAAAAATACCACAAGGACATTGTAGAGTTGATGGTTCCTTCATCCTGGAAATGTAAATAAGGATTAGGGAAATGTCAAAAAT
AGTTGACATTTGTACAAAAATATTGCAGGAAAGTGCGTGACTATTCTGCCGGTTGTCACCATCCAAAAATCCAATCAGTTTTTCAAATTTGCCATAGCCC
ACCCTGCCTGCAACGTCAGCACGCCCCAAATTGCCCCTTTGGCCTTTTGCTTATTTATATAATGGTGGGTTTACTTGTTTCAGTGGTGATGTGGAAAATA
GTCCCCAGTATCCCTTTAATTGTGGCCATCCACATGTTTCTGTGGGGCTACACAGGCTCATGGTATCCAGTTTGGTCAGAAAGGATTTTTTTTTTGATGC
TCTGAGGCAAATTAAAGTGTTGCCTTCCTCTGGATGGGCTTGCGGTTAGCTTTTACAAAAAGTTGATCTTGAGATGAAAGTGTTTCTTTTTTCAGGCTAA
ATTACTGTGTAAGCAAATTGGATGTCATGGTCGCAGCTTCATTCTCTTTTCAAAAAATGTATAGAAATAAAATTTCTGTTCTGAGCTACACATGCCCACC
AGCCAATCAGAAGGTGATCTGGCAGAGGGGAGGGGGGGGAGGGAATGAAACACATGTGCAGTATGAAGCAAGGAGGGAGAATACCTTTTTAGAGATGGCT
GTCTGTTCTAGAAAATGTGAAGTAAGTGTGACTGAGTAAATATGTGATTAGGTGAGCCAAAAGTGTGGCGTTTTTGCTAAACAATAGGAGGACTATTGGG
CAGTATGCTTTTCACATTTTGACTTGCATTCTCCTTTAATTGTGATTGCACAGCTCAGTGCTTGCATATGAGCGTCCATACACCTGCTGATAATAGATAT
TAGGCTAATGCCAGACGAGGAAAGCGCTTGGCAAAAGTTCCGCCCTGCGCCTCTTACTTGTGCCTGCACTCGAATGGGTGGGGTGCGCTCGGGTGCAGGC
GCATGTAGTGGAAATACGCATAAAACGCAAGAGTTTGAAAGTCTCGCGTTTTTGTGCGTGTTTCGGCTGCATGTGCCTGCACCTGAGCGTGTTCCGTTCA
TTTGGGTGCGGGCGCGTGTGGGAGGCGTAGGGCGGTATTTTCAGCAAGCGCTTTTCCGCTTGCCGAAAATATCCGCCCTACCCCTCGTCTGGCATTAGCT
TAAGTTGATATGCTTCGTTTTTTGAAGTCACCTGAACAGCTGAAGCATCTCGTATGGTTTCCCACTGGCGATTTACATTATTGGCAATGAGGAAGCATTT
TGTCATCTGTGGTAGCTCAGATTTAACCACAGGCAACAAATCTCCCCCATCTAAAACACACTAAGGGACAATGTTCCTAAACGCTTTAGTACTATATTAT
CAGAAATGTGCCCCCACTCATTTAGTGGGACAAACATTCTAAATGCCTTGAGGCATTTAGATTATATACCATAATAGATAAATATTGGGATGAAGCGAGA
GAAAAAAATGTGCAACATCGTATACAGTCTGCAACTGTGGTTCTGGGTCAGGAGTGGAATCTCAAACCATCCAGTTCCAGAGAACTGGTAACCGATATCA
TGTTACTTGGAGAGAAGTGGACACCATCAGAGCTTCTGAGTAACTGAAGAAATCAAAAGATGGAGACTCGCTTTATGCTGGCTTAAGACTTCAGGTGAAC
ACAGGATTTCTGTGACTGTCCCTGTGTGTTCTTTGTCCCCACACTGAAATACAATCTGCAAATACTGCTTGTGGCCAGTTTACGAGGTATCTTCTTCCTC
TGTGCAAGCAAACCCAAAGAGGCCTACATTTTATGTGAGTGTATATTTATATAATAAAAAGTCACATTGCACTATTATGTCTTTTTCTATTAAGTTACAT
TTGTAGATTTGACATAAAAAAACAGCAACACCTCGTTATGTTTAATTTACTTCTTTCTATTTCTGCTATGTCATTGATCAATAGGGAATTACCATCAATT
ATCATTTATGTAAGTACATATTCACACTTTCTGTGTATTTCTGTAGCTCACACAGTTTAAGCATTAATCATATTATGCCTATAATCTTGTACCTTTTCTC
TCTTCATATAAATAAACTAAGGTGAGTCTTTGTTGTGAACTGTATTAAATGCTCTTCTGTGCTATTAATTGGGATGGACTGGTATTAGTATAGGGAAAAC
TGTTCACCACAGCACAATAAGTTTTCTTTTTCCCTTAAACAGAGCTTTTCGATCTGCTGCAGCGGCTGCAGGCATGGAATTCCGCTCAGACAAATTCTGG
GAAATGTATATAGACTGGGAGATAAAACAAGGGAATTTCAGAGAAGCTACAGCAGTCTATGACCAAGTACTTAGTATTCCCACCCAACTGTACAGGCAGC
ACCATGAAAGATTTAAGCAACATATCTCTGCCCATGCACCTCATGAACTTCTTAGAGAAGAGGAGTTTAAGTGGATCTGCTCCAAAATCAAGGCTGAAGG
GGAAAACGATCAAATTGCTGCAGAAGACTCTCCATCTGGTGATGACCAGGAGAACCCAGTGGATGTCACAGATCCTGAATTGCAGTCCAAAGTGAAAGCA
CAGGTTTTAATTATAAGGGAGCAGTTATTTCTTCTTAATGAAGCAGAGGTCAGGAAGCGGTGGAGTTTCGAAGAGGCGATCACACGTCCATATTTTCATG
CCACGCCGCTAGATCGTACCCAGTTGCAGAACTGGCGAAAATACCTGGATCTTGAAATATCTCAAGGCCGCCATGAGCGCATAGTGACATTGTATGAACG
CTGCCTGGTGGCCTGTGCTTTATATGAGGAATTCTGGCTGTCTTATGTCCAGTATATGGAGCCTCATTCTATTGAAGCTGCCCGTTGTATATTGCAAAGA
GCATGTTGTATTCATTTGCCCTTGAAACCTACTCTGAGCTTATACTGGGCAGCATTTGAGGAAAAGCATGGACAAATCGACACTGCCCGTTCAGTCTTGT
ATGACCTGGAAAATTTGATACCTGGACTTGCCATGGTTCGGTTAAGGCGCGTGAGTCTGGAGAGGCGGACTGGGAATTTAGAAGAGGCCGAGCATCTGTT
GGAGGAAGCTGTGAAGAGCAGCTTGGGTACTGAACTAGCTGCCTTTTATTCAATCAAGCTGGCAAGGCTACTACTTAAGCTTCAGGGAAACATGGAGAAG
GCAAGAAAGGTTCTAACTGAGGCTTTGGAGAAAGAACCGGTAAGTGGGGGAAACAAGAACACACCAAGGGTGAGGACACACATACTATGTTATGCCTATT
TCAGCCAGCGGAACAGTCACTACAAATGGGCTTTTCCCAAAAACACTTGTTAGTAGATGTCTCCTGGCTGAAAAGTGCATACTTTGCTGTCACACCTTTC
TGTGTTTATTAGAATGTTAAAGTGGTCTTCCACCCAAAACCATGCATTTTGCATAGTAAAAAAAAAAAAAAA
5' EST: CX891509 [+]ggcaagatggcgacagactggaggttgccgaaagctgtatgtgcagaagctgaagttccctttaccactgaggagagtgatgatgagggcgaaccaagtg
atccaaaagagccagaactgccggctgagtttaaaacactttgggaggaagccacagagaacccatacaacttcaacggctgggcaaaagtgttggagta
tgttgaaacaacgaataaccttgtggcaggcagaaaggtctatgatgcttttctcacacgctttccatattgttatggctactggaagaagtatgctgac
ctagaacttcagttacgtaatacagctgagacagaagaggtctactgccgggcactgcagtctattccgcttagtgtggatttgtggataaattacataa
catttcttaaaaacaccctggatacagcactgccagaatccattgagaaactgcaagggtgagagagtaactctctgtctgtctgtgattgtgaggttat
aagaatgtaatttgtgtgaactggaggcaaccatcattacgtcaggaaagaaagtcttaattttgtttcctgttataaaaacaagttgggtttcattatt
tctagaggtgtgtagtgtttttataagtgaataccacagtctgtgcagatcatatgttttgatctttatagtattaaactgaaattaaaaggggtagtta
acct
3' EST: CX891508 [+]tttttttttttttttactatgcaaaatgcatggttttgggtggaagaccactttaacattctaataaacacagaaaggtgtgacagcaaagtatgcactt
ttcagccaggagacatctactaacaagtgtttttgggaaaagcccatttgtagtgactgttccgctggctgaaataggcataacatagtatgtgtgtcct
cacccttggtgtgttcttgtttcccccacttaccggttctttctccaaagcctcagttagaacctttcttgccttctccatgtttccctgaagcttaagt
agtagccttgccagcttgattgaataaaaggcagctagttcagtacccaagctgctcttcacagcttcctccaacagatgctcggcctcttctaaattcc
cagtccgcctctccagactcacgcgccttaaccgaaccatggcaagtccaggtatcaaattttccaggtcatacaagactgaacgggcagtgtcgatttg
tccatgcttttcctcaaatgctgcccagtataagctcagagtaggtttcaagggcaaatgaatacaacatgctctttgcaatatacaacgggcagcttca
atagaatgaggctccatatactggacataagacagccagaattcctcatataaagcacaggccaccaggcagcgttcatacaatgtcactatgcgctcat
ggcggccttgagatatttcaagatccaggtattttcgccagttctgcaactgggtacgatctagcggcgtggcatgaaaatatggacgtgtgatcgcctc
ttcgaaactccaccgcttcctgacctctgcttcatta
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: