XB-CLONE-3279130
Clone Name: IMAGE:7679007
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Gene:
med14
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX893178 [+]
aagggggccgctgagaaggcctgggtctcgtataggttagtgtttgggttttgtagggaacccttgcccaccacagcccggcacacagtacagttctagc
gcttggagatcataagttaccgtccgcttaacagaaaggataagaggaaaggaagaaagctaacttcgactgctttttcggatctcagtctccccgccat
gtctccggagcggctgttgcatacaagctgcggctgagattgctggttacctgcttattttttcaccccgcgggcatcttccatatccagctgggcctgg
gcagcccttggtgctggctttcattcaaagcgaagtgctgctgtcttagttttagcccctgaaccccttcccctcctcttctaccccgggttatctgtgc
tgtgtttcccatcctgggtgcagtctggtggtaatagaggacgggggaggtttctatatattgatttctacattgagactgtgtctgaataatcatcttt
ttgttaacctttcatatggttgatttgatgagcagcccacttgtattattttattataacacaactttagagtctgcattatttattttgtagtctgttt
ttcttcgttttgaagtgtggctcattttaattgaatgttcgttctttagtcatagtctatactccttcattattatataaagacttttttttatttcacg
cccgtccacccttttctggtgatcactgttaaatatagccacggt
3' EST: CX893177 [+]ttttttttttttttttaagtttttgaacaatttatatatatatttatagatatatatatagatgaagtgcatctgagaaaatatctgccagtttttcttt
taaataaaaatatccaaaggcaaattgtctcttgttaatccatagttagagtctgaggaaaaactttgcaaactgcgaaatagtgttcagggtcgaccac
cgggaggcaatgtgagattggccattaaatctcgtactgcagcaaatattgtacattcacctggccgtggtggattcatctcagcaaaccttttcagaat
attgctaactgttaagggagccgttactgaagagttctgctgccgagggatttctgcttgctgggttgttcctgatgccatatcataaataataggaaca
ataatactgaaagcatctgttggagctgaagtcttttgagttagctgaagaaaaaacaacattttagatttcagtactacagcaggagttcccgatggag
ctattggtggtgcactaggtgggatggttaggcagaactgcacattccatttcagttgggttgtctgatctggaaacagttctagcttcattatatgcac
acagtctctgaggatatgcgcaggtgctcctaataacttggtgaaagctatcaaagtatttgccttgaatggaggtcctgcaaccctggtctcaaagaac
ctctctaaaacttgaagctcttctggcttccactgtccagcattctcaggtgtaacttttagttgcaaagtttggttg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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