XB-CLONE-3263927
Clone Name: IMAGE:7680024
|
Gene:
prdm10
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX895667 [+]
tttccttagtcagtgtggctgtagtcagtaggttagttagggcctctaaaacgcgtacaatgcaagctggttttagttaataaagcccattttggaattt
cgtcactttatttcactttggaatttatatctttcggatccgcacagattaatacatttaactttgaaacttatagggagctatagttaagtaattcatt
atctatttggtacgatttaagtaatttgatgttgtacaaaagttcgtgcaaaaaagccagagtacccctcatgatctatatatccgcaattagtagctga
aacaattttacagatattccactagaataaggatcatcttgactgaaagatcatatttatttatggtcttgataatctgttaacacagttactatgtagt
aatcctgattttcaataaaattcaaggcaagaggaatgacaagtagattaaaaggctactggcttggccctgcagacaaggattgctttatgactggttg
taattcaattttttttttcaaaaaaagccatgttcgtgattttagtcacagtctgcacaagtagaaccattttgaacagatcaaatagcaactttgttac
taaatgggagcattaaactaagcaatgatggacaccaaggaaggaagtccacatgtgtggcctacttctgtggagcaccagtcaaatactgctcaggtac
attttgttcctgatggcggatctgtggctcagatcgtgtacagtgatgatcaagatcgtgctcagcagcaggtggtctacacagcagatgcctcatcngt
tacatctgtggatgcatctgaacatacgctggtgtatatccacccagtggatggatcgcagactctctttacagaccagcctca
3' EST: CX895666 [+]tttttttttttttttaagagataatatggatcgaccttactcatagtaaagttatgtccccttcttttagaaagtggcttttaaatatgtgttggatgac
caacacacattacagttaaacaatttgagagaataaattcaaggtgcttttatgcttcatatcctttttacagtgttatttcctacaacattagaaattg
ctggtagggtaagtccagaaacttgacatcattcccagttaaagggatattaagatcttaatgcaaagcttcgtgctggccatacagtgttgcatgtaac
tacaaactgaagactttcaaagcagcaacaaataagcatatgttcatgtttagatgtgtcactgtcccattgttttttgttctctgccttctgcaggtat
attgcattgtcccaaggttaacagttcaaaagctttacatctttaaatacttttccagggacagatgaagacagacaccctgtgtgctcacgattcaaaa
gttttgtattaagaaaaattaatgtttttttccttttggctccttgtctgttcatctggttttccgcactctcatatttttcagggtttgctgatgtgta
cctcactgctaccattcatgttggtagttgtggtgattatgtattgtgttgtttgagatgaattgctggctggctgttccatatggtcagggtttggagt
tgcctcctctgcaagagaactactcttcttcatatctaagactccttgtccttcagatatcacataatgagtctgagtgactgctttaacttgtccacta
ctgacaggggtaacaggtgtgcctactgcagcctctgcaatcacatactgcccttgaggtaatgccatcatctgaaattcagaggg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: