XB-CLONE-3263274
Clone Name: IMAGE:7680752
|
Gene:
neo1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC167600 [+]
gtgcctgcagctccgtgctccggtgtttgcggttggatttctgtacagtaggttagcgcacaggcccgtacaactatttgtctctgctcccgggatggtg
aaaagcgggtcgcggtgaaaaggtgtgggatggcgatccgaagccccccgggttcgcttgttcgggtcacccgggggctgttcgccgtctccatcctttt
ttccgtgtggaagaagccgacttgggctgccaagactgcaggctcaggattcagaacctttacacccttttatttcctgacggagccagtggacacagtg
actttgcggggttttgctgcagtactaaactgctctgcttatgcggatccaacgccgaagattgaatggaaaaaggatgggacatttttgaatcttgtgt
ctgatgatcgccgcaggctcttaccagatgggtcattgcttataacaagtgtcgtgcactcaaagcacaacaagccagatgaaggcatctaccagtgtgt
ggccactgtagaaagtctgggaagcatagtcagccgcactgcccgactctctgttgcaggtctccctcgatttactagccagccagagtcctcttctgta
tatgttggggatagcattgtattgaactgtgatgtcagtcctgaactggtgccttttgtgcattgggagcaaaacaaggcttcacgagaattggacaaca
gagtagctgtcctgttaaatggcagcttggtcattagcaatgccaatgaaagtgatgctgggctataccgctgtgcagtaggtggtgggtcatccattaa
atacagtgaggaggcagagattagagtacttcaagacacaggagacgagcgccccatgctgtttcttcgccagcctttgccacttaccagagtttctggg
cagactgcactcttgacttgtgtggtatctgggtatccaacacccagtgtgcgctggacccataaccagaaagaaatactgactgaaaactcagagacgt
taacattcgtggcagggggcagcctgcagatcagttctgtaacggaagaagattctggaatttacacatgcattgccgacaatgggaaccagactattca
ggctcatgctgagcttagcatacaagtaccgcctattttccatgtcaagccatccaatactcatgcacacgagtccatggacattgtatttaagtgtgag
gtaaccgggaaaccgacacccacagttaaatgggttaaaaatggagatatggtgattccaagtgactacttcaaaatagtggaggaccatgacctgcagg
tgcttggtctggtgcgctcagatgagggattctatcagtgtatagctgaaaatgaggtgggaaacgtccaggcagcagcacagctcattatccttgagcc
tggtgttaccatcccaacctctcccaccccatcactgacccgtgccactgctcacaatgtagctgccactccaggaggccccctgccttcatccccaagg
gatgtcgtagcctctctggtgtctacacgtttcataaagctgacttggcgtatgccggctgatccacatggagagaatctcacctatcaggtctactata
gcaaggaaagcatgaatagggagcgtgtggagaacacgagccgcccaggagaaatgcaagttaccatccagaaccttctacccgagacagtctattactt
tcgagtggtggctcaaaatcagaatggagcaggtgaaagctctctgcctctaaaggttgaaacacagcctgaagtacaagttcctggcccagcccccaac
tttaaagccacaccagtctcacccacatctgtaagtgtctcttgggaaaccccactgtctggaaatggggagattcagagctacaagctttattatatgg
aaaaaggagcagacaatgaacaggatgttgatgtgggtggacattcatatacattaaatggcctgaaaaaatatacagagtataccttcagggtggtagc
ctttaacaagcatgggccaggtgtatccacgcaggatgtgactgtcaggactctgtcagatgtacccagtgctgcccctcagaacctgacactagaagtt
cggagttcacagagcattctagtgcagtggcagccccctcctataggttcccaaaatggacagattgtgggctacaaggtccgttaccgcaagacaacaa
gaaagagtgagactagtgagattcttgtggatgctcagctatcatatcttttcacagctcttgagcgggacaccgagtacagttttcgggctgtggcctt
gactgttaatggaagtggcccagcctcagactgggtttcagcagaaacctttgagagtgatctggatgagtcgcgtgtaccagatgtgccaagctccctc
catgtacgtcctttggtcacaaacattgtagtgagctggacccccccagaaaaccagcaccaggtggtggttcgtggatacgcaattggctatggcttag
gaagtccacatgcccagactgtacgtgtggaccacaagcagcgttactataccattgataaccttgaacccagctcccactatgttatctcgcttagagc
ttttaacaatgttggagagggaattccactgtatgagagtgcagtcacccggcagcacacaggtaagtcttagaaacatggtgctctgaatgatgggagc
aggatggtgaaatgtagggcatttggttctcatttaaagtttggaatttgctaaattctgtgtcctgctcagtgtgtgtgtgcttattactggtaacatt
gctgcttctttcttgttactaacagtctcttgttctctctccctctctcctctttctttttgctttaataatcattgatttatccacattccttgttaaa
tttatcttatgttcttaaaatctttactggattttaagaacataagataaatttatacatcatacacttctcttccattccatcaattcctgtacatctc
acatcccctaatcctgcctatggtcgtggcctttttcttttatacaaccctccccccccccaattctttgatcctataacattcactcctgcctgttcct
gcttacatcctgtgatagacccttccgatgttgatgtgtttgttgttcatgccccatacaccccagttccagagcccagtcctatgttgcccccagttgg
cgtgcaagccactatcgtggccagtgacactgtgcgtctcacatgggcggataactctctccccaagaaccagaagatcactgattcaagatattacaca
gtgagatggaagaccaatattccagcaaataccaaatacaagatggcaaacagcacaaccttaagttacttggttactggtctgaagcccaacactttat
atgagttctcagtcatggtgaccaaaggtcgtagatcaagtacatggagtatgacagcacatgggaccacatttgaatcagtgccttcctcacctcccaa
agatgtaactgttgtgagcaaggaaggaaaacctcgcaccattattgtcaactggcagcctccctcagaagccaatggtaaaataacaggttatattata
tattacagtacagatgctcaggctgaactccatgactgggtgattgagcctgtgattggaaaccgtctcacccaccagatacaagagttgactctagaca
ccccttattattttaagatccaggcaagaaattctaagggcatgggtcctatgtcagaagctgtgcttttccgaacaccaaaggagtcatcaccttacat
gcctaacgaccaagcatccagcatctctgggaaaggagttcgcccttctgatcctggatcaaatggtggctcaagtcttggaggcagtaatagtcaccat
gggaacccaacgtcactggatcagaacatgcttttaacaattattgtttccattggtgtgatcactgtcttgattgctgtggtcatagcagccatatgtt
cccgccgttctagctcccaccacaaaaaaaagagagctgccagtaagtcagtgaatgggtcgcataaatacaaaggcaactccaaggatgtgaaaccacc
agacctgtggatccatcatgaacgtttggaattgaaaccaacagacaagtccccagaaacaaatcctattttgacagacacgcctctgccacgctcccca
caagatctcacaccaggggatagcaccacggaaagcagcatccaccagcacagcaattcgtatcgaggtctggaatcagaagattctgtgtcctcacttg
ctgggcgccgtggcatgcgacctaagataatgatgccttttgactcccagcctccacaaccggttgtcagtgcccatcccatccattcccacgataattc
tcatcattttcgctccgttcctgggttcatgctgcacgtgttgggtggccctccacaggctgcagaatctgtgcgtcatacccctagctgtgacactgta
gtagctgcatcatcttcctctcagactggccctgaagatccagacccctcgggggttagtttcttgcctggctccctggatgaagattcagtacctagtg
ttcccactgcacatatccgtccttctcatcccctaaaaagctttgctgttccagctgtgcccacctctgcttctggagcttcgtatgaaccctcattacc
tagctctccgcttcttcaacaacaatgccccaccgctctgtctgtaaagacagcctctcttggaactcttggcagggtgcgcccccctcttccagtcaca
gttcccagtgcccctgaaccactagagacaagcaggatgctagagggctcagagtgtagctatgaaccggacgagttaagcaaagagatggctcaccttg
aaggcctgatgaaggatctaaatgccattaccacagcatgagaaaaatagtgtctcaccccagtggcctgtcaatgaataggcattcagctcggtgccag
ctaaaggcaggagctcatgtgccacaaccacctgtacagactagaggggtgtaagagcccagactgtgtatgctgagaaccaccaagggccaaagttcag
tactttttttcttgctattctcataccctctgatttctaagatgaatttttttcttggagggaggatttatataaaaaaaaaaaaaaa
5' EST: CX896781 [+]tgcctgcagctccgtgctccggtgtttgcggttggatttctgtacagtaggttagcgcacaggcccgtacaactatttgtctctgctcccgggatggtga
aaagcgggtcgcggtgaaaaggtgtgggatggcgatccgaagccccccgggttcgcttgttcgggtcacccgggggctgttcgccgtctccatccttttt
tccgtgtggaagaagccgacttgggctgccaagactgcaggctcaggattcagaacctttacacccttttatttcctgacggagccagtggacacagtga
ctttgcggggttttgctgcagtactaaactgctctgcttatgcggatccaacgccgaagattgaatggaaaaaggatgggacatttttgaatcttgtgtc
tgatgatcgccgcaggctcttaccagatgggtcattgcttataacaagtgtcgtgcactcaaagcacaacaagccagatgaaggcatctaccagtgtgtg
gccactgtagaaagtctgggaagcatagtcagccgcactgcccgactctctgttgcaggtctccctcgatttactagccagccagagtcctcttctgtat
atgttggggatagcattgtattgaactgtgatgtcagtcctgaactggtgccttttgtgcattgggagcaaaacaaggcttcacgagaattggacaacag
agtagctgtcctgttaaatggcagcttggtcattagcaatgccaaatgaagtgatgctgggctataccgctgtgcagtaggtggtgggtcatcccattaa
tacagtgaggaggcagagattagagtactttcagacacaggagacgagcgccccatgctg
3' EST: CX896780 [+]aaatcctccctccaagaaaaaaattcatcttagaaatcagagggtatgagaatagcaagaaaaaaagtactgaactttggcccttggtggttctcagcat
acacagtctgggctcttacacccctctagtctgtacaggtggttgtggcacatgagctcctgcctttagctggcaccgagctgaatgcctattcattgac
aggccactggggtgagacactatttttctcatgctgtggtaatggcatttagatccttcatcaggccttcaaggtgagccatctctttgcttaactcgtc
cggttcatagctacactctgagccctctagcatcctgcttgtctctagtggttcaggggcactgggaactgtgactggaagaggggggcgcaccctgcca
agagttccaagagaggctgtctttacagacagagcggtggggcattgttgttgaagaagcggagagctaggtaatgagggttcatacgaagctccagaag
cagaggtgggcacagctggaacagcaaagctttttaggggatgagaaggacggatatgtgcagtgggaacactaggtactgaatcttcatccagggagcc
aggcaagaaactaacccccgaggggtctggatcttcagggccagtctgagaggaagatgatgcagctactacagtgtcacagctaggggtatgacgcaca
gattctgcagcctgtggagggccacccaacacgtgcagcatgaacccaggaacggagcgaaaatgatgagaattatcgtgggaatggatgggatgggcac
tgacaaccggttgtggaggctgggagtcaaaaggcatcattatcttaggtcgcatgc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: