XB-CLONE-3261261
Clone Name: IMAGE:7682872
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Gene:
arhgap4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC167605 [+]
actgcttgcctgactggaggttagcgctcactgcttgcctgactggaggttagcgctcactgcgctgcttgcctgcgctttgcgccctgcgccaccggac
accgtatctgtcaatagggatgggcatcggattgggcagtaccgcctgcatggatcttacactgtctgcttgataacactgcagcccagggcacagcagc
ttctcccggagtaaaagccacaccctttagcgggcactaaagggtacatcgtccctacgctttggatgtggtgtgcagggattctgtccttcccagcgct
tggggggcgatagcagagccgagtaagagtattcagtaccggggggcacggaatggcatctcatggaaagccccggagggaccgggcatcggagtatgag
aaccagatcaaagaaatccgggctcaactgactgaccaggtcaaatgcctggacacgcaagtggacttccgccagcagcttctgcaggacatgagcgaat
tcctacgcagaaaggcagagatcaatctggagtactcccggagtcttgagaaactgagtgaccgcttctcttcaaaaatccgcaattccaaggatcacca
tatcttcaggaaagaacagaacctgctgtctcctgttaattgctggtatacaattatgaatcatacaagacaggaaagtcgggatcatggagctcttagc
gacgtgtacacttcacatctcatcccacgccttgcacatatcggagaggatttgactcgtctttcaaaaaagagtaaggaagtaggtttgcagcaacagg
aggatttgttaaagttggtttccgagttacagggggccatgaagacgtaccagttgtatcacagtgactgtatgtatgctgaacataagctcaaggaggc
tgagaagcaggatgaaaaacgcacaggcaaattaagtgaacatatggcaagttcaccagtatctgacagagggcagagacggagttctctaaagaagacc
gaaaggcttgtagaaaagaggctgaagaaatatttggagaacagtcagaagtgcaccaaggccaggaatgactatctcataaatctgaacactgtcaaca
ataacattgagaactactatatacacgatatcaatgacctcatcgattgctgtgacctcggattccatgtttctatgagtaagacaatgcgagtttacct
gcaggctgaggagagagtgcagatgtcccgtcagcagagtcttcaggctgtcgagggagctgttgacaacttcaattcacaggcagacaagaataaattg
tttgagatgtacagtgaaacattttcccgtccaacaaaatttgaataccagccacatgagggggatgaggtttcggaagtccggtctgagccactgttac
atcaggaattggtctctaggttacagcatataaaatccagactggatagcgagcacctggaaactgaagaggtgaaaaaaacccttaaagccactttaca
gtccctgttagatcttatcggaatagatgagtttgacttgtcagaggttttccagtccagccagtctactgaatcccttaaatctacaggatctgacacc
agcactaagacaagcacagccaagaggagagctaatcagcaggatactgagaacttctatgtttcaaaattccagaagtatctacttggaagaggtacaa
tctgcaaactccaggcaaagtatgacctgctgacggaagccatagaaaaagctgctgtgatggacagtaatatttctgggcagctgtccagcaccagtaa
gtctctgcgagtgaaaaggcagcgaccgacatcccagtataaccacaaactttttaacggggatttggaggaatttgtccagagttcaggacaagccatt
ccccgggtggtatccagctgcattcgcttcattaacttgcacggattgcagcatgaaggtattttccgtgtcccgggatcccaggctcaagtgaacgaga
tccgaagttcttttgaaagaggtgaagatccgttggatgatagctgcggtggccatgatgtggactcagttgctggagttctgaagctttatttccgtgg
cctggagaagcctctctttccccctgagatgtttaacgatctcctgtactgtatccaactggaaacacctcaagaaagaataacacacctaaagaagctt
gtgtcccatcttccgccagttgtaactcttgtccttagatacctttttgccttcctgaatcatctgtcccagtacagtgatgagaacatgatggatccat
ataacatagcagtgtgctttggaccaaccttggtttccatccctgaggggcaggaccctgtatcagttcaggcccgtgttaatgaggttgtgaaaactat
catcatttaccaagagaggatatttccaggaaatgctgaactagaggggccagtttatgagaagtgtatgacggtggaggaggatgaagagtacagcgat
ccactggtccttgatgctactattgatgaatgtgaacaggaacaactgcaagagctggccctcagcgaggatgagctggagcctatagaatccattgctc
gatttgactattcagctcagacacctcaagaattatcattcaaaaagggtgacattctgctacttcatggcaaatcaacaggagactggtggcgaggcga
gatagcagggaccttggggttgatcccacacaagtacatcagtattcctgagggagcagagaaaaggctggagcaaaggggccggaaaaaagagctggac
tctggtacagcccatactcctgaagatggccttaaggaaccatgcaggcttcgtgtaaattctgacagcgcttgctcccctaggaaacgcagtgaaggaa
gccccattaggaaacttaactctcccttcatggataatgttcggcttcccttccctttggctttctcggcaacccccaggacggggggacatattgaaag
gattccaggtactggagaaaggagaaacttaatagacagtgcttcaaagagtaccatcgcaaccaaagcctggcaaaatgatcgtcagggcattgaagtg
gataagaatgtgacaaagaacatggacagcgtgttcaaagagctgcttggaaaaactgctctaaagcaggtgcatttggaagatcctgtggaccctggta
aagcaggaaaatcacgggaacttaaacaagtgacaccaagtacctccgatcaatcgcgtggaagcccagcagggaaaaaaggagctgcaggaagaactgt
gtttgggacccggagatgaacttcagctactgatttctaaatgagggcaagaagcatggtacagaccagtgggttccttggtgggagagattgttcagcc
atatccatgtgtcttatggcctcactcagactcataaaaatgtaatacacataggatgcttgctcctgttgagagtgaatggaatgtgccctagaagggt
tcaggactctcttaagctctcctgcacatttgttttactttatcaatgtgtgtacaaatgtcatgaacagaagcacagctataacgccagcaggggcctc
ccttacattttccttccccaaacttgccagcactggactcttacggtcaacactgtaagcaaacaggcgggagtatcatttacagaagtggttgaaatta
aaaggtccctcaaagccaccgtagcgcctcagaattttctgagacataagcttgccaaacataccacaagtatattagtgctgtagactgaaagcaccct
cagaagcacaatcttaaagaaatttagacatgacttttctagtctatagacttgccaacattaaaaaataaatcagaagtgttggagaacttttgctgta
ttaaaaagatacaatttttattagaaggaaaagtctgagctgttgtgtacttgaaagtcttccagtctatgggaacagctagttggttcgttgtgaggtt
aacaacaccgaagagcagcacaagcctgttaatatttactaggaatttttatattgcatggtaagcaggggttaataactattgggcctcccgatgtcat
ttcagttttaagagtgttcttcaccaaattgatgtagcgtttatttaggtaactgcacaccccctaacagcaaatcgattcaacctgttctggttatact
tatccaatcattgaatatttgaagattgcaaacctcctgttagtgatcagtccatttgtttgtaagtctgcatttgcacagaattggtcactgcaacatc
accagcaagatacaccaaacaaggtaaacagctttttttttatttaccccattgtcacagggatcaagggttttgaggcagcttatgggttaacagatgt
tcccaatgtgaatggcacaaaagatgaagcccaatttttatttagtttgtccactttccttggactccaagactttgtaacaagactgctgtgtgtctgc
tggcccagtcccatcctcttcagtcctgtttaccctgtcagttctttattgtgcccagcctggacctggtcactgagtgacctactcaccaggaaatcca
caatcctttgcttgaagtgtcatcatggcactcagcatatgtatataagttattctgtgtgtgtgacagaggagaagagccttatatttcaatcatttgt
tgttatttttcactgatcttgaaaactgtgtcagtctgttttttttttaaaagtatggagggggcggaattaaaatctggctgctttaaataaaggctgc
ccacgtgaggattctaagaagtacattactactctggcccgttcattgcacttttggaatctctaactcaggaatacacaacctgtgcctctccagctgt
tacagacttaaaattcccagaaggctttgaagctgctaaaatatggaagatgttgcatgacagcagttggagagccatagatgggtttcctgctccatat
ctcctcattgtgtttgagtaccagaaaagtattcttacgccctgctctggttcccattcaagtccagctatttgtgcttataaagcaacgctaataaaaa
tgtgtttataaccaaaaaaaaaaaaaaa
5' EST: CX900319 [+]actgcttgcctgactggaggttagcgctcactgcttgcctgactggaggttagcgctcactgcgctgcttgcctgcgctttgcgccctgcgccaccggac
accgtatctgtcaatagggatgggcatcggattgggcagtaccgcctgcatggatcttacactgtctgcttgataacactgcagcccagggcacagcagc
ttctcccggagtaaaagccacaccctttagcgggcactaaagggtacatcgtccctacgctttggatgtggtgtgcagggattctgtccttcccagcgct
tggggggcgatagcagagccgagtaagagtattcagtaccggggggcacggaatggcatctcatggaaagccccggagggaccgggcatcggagtatgag
aaccagatcaaagaaatccgggctcaactgactgaccaggtcaaatgcctggacacgcaagtggacttccgccagcagcttctgcaggacatgagcgaat
tcctacgcagaaaggcagagatcaatctggagtactcccggagtcttgagaaactgagtgaccgcttctcttcaaaaatccgcaattccaaggatcacca
tatcttcaggaaagaacagaacctgctgtctcctgttaattgctggtatacaattatgaatcatacaagacaggaaagtcgggatcatggagctcttagc
gacgtgtacacttcacatctcatcccacgccttgcacatatcggagaggatttgactcgtctttcaaaaagagtaaggaagtaggtttgcagcaacagga
ggatttgttaaagttgggtttccgagttacagggggccatg
3' EST: CX900318 [+]ataaacacatttttattagcgttgctttataagcacaaatagctggacttgaatgggaaccagagcagggcgtaagaatacttttctggtactcaaacac
aatgaggagatatggagcaggaaacccatctatggctctccaactgctgtcatgcaacatcttccatattttagcagcttcaaagccttctgggaatttt
aagtctgtaacagctggagaggcacaggttgtgtattcctgagttagagattccaaaagtgcaatgaacgggccagagtagtaatgtacttcttagaatc
ctcacgtgggcagcctttatttaaagcagccagattttaattccgccccctccatacttttaaaaaaaaaacagactgacacagttttcaagatcagtga
aaaataacaacaaatgattgaaatataaggctcttctcctctgtcacacacacagaataacttatatacatatgctgagtgccatgatgacacttcaagc
aaaggattgtggatttcctggtgagtaggtcactcagtgaccaggtccaggctgggcacaataaagaactgacagggtaaacaggactgaagaggatggg
actgggccagcagacacacagcagtcttgttacaaagtcttggagtccaaggaaagtggacaaactaaataaaaattgggcttcatcttttgtgccattc
acattgggaacatctgtt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: