XB-CLONE-3261124
Clone Name: IMAGE:7682957
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Gene:
tpra1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC167606 [+]
cgatgagccgctggcatttagagtggaagagaaagcgaagacggagcgggcagaaggcggggtgcagagaggagccggctggctgcagctggattgtaag
ccgcgcacaagccttgttcctcaagaggaagtgaagccctgcaccctgttgtgttctgactggcctctcgccttctcctatcaggctcaggatgtccgca
gacaccaacagcagctctggcctgatcctcaccgctactcccagctctacatcccttgtgactagcgtgcagccaacctccagtactattttcaacagca
ctaacatcaccataccccatgtctgcttggtcattctctttgaagacattgggaattccagggtcagatactgggatctcatgctgctaatccccaatgt
tctcttttttgtgtttctgctttggaaacttccatcagcaagggccaaaatccgtgtgacatccagccccatttttaccaccttctacatactggtattt
gtggtggctgtggtgggaatcacacgggccattgtttccatgactgtcagttcttccagtgcagctacagtcactgacaaggtcctttgggaaatcacca
ggtttttcctacttgcaatagagctcagtgtggtgatcctgggcattgcttttggtcaccttgaaagtaaatccagcgtgaagagggttctagcaatcac
tgcagtctcatctcttgcttactctgtaactcagggcacacttgagatcctgtaccccgatgcacatctgtctgctgaagatttcaacatatatgggcat
ggtgggcggcatttctggcttgccagttcctgctttttctttctggtttataccctggtgtctctactacccaaaacacccctcaaggatcgcatctctc
taccatccaggaagagtttctatgtgtatgctgtgatcttggctgccctgaacctggtccagggaataggaagtgccttgctgtgtgctgatattataaa
tggattatgtcttgtggacgtcactacgttcctgtacttcagcttgtttgctccactcatgtatgtggcatttttgaaaggcttctttggatcggagcca
aagatcctcttctcctacaaatcccaggtggatgacccagatgaaactgaggttcacctccctcacccctacgctgtagccaagaaggagggcatagaca
ctggcttctactccaacactcaaatagacaccacagcgtatctcgatgatgtggcctctatgccccatgtggtagggagcattaacagcattgacagtga
ccgctggaaagctataaatgcttagtgttggacagcacattggtgccagaatgtctggatcataaagttttaaacttcagagactgaagtggttattccc
atggaaaggcattggtcctgaattctccttttccaacatcacccgaggaacaagatcaaccccatctacatacaggagaggccagagcaagtgctgctaa
acctttgcactgtgtttccttgctgggttgcactgacaaagcgtagatgatattattcatctctgtgcacccttcgctgtcatagggcaggcgaacaatg
gcgtactattccacagcaaatctgtcagcccgaaaggatcagtattctatgaataaggcaacatttccttcatacgtatatacagtggtcttcagtcctt
aggagccattggctgaattcgaatgactcatctgtgctcctttaaatgtcttctcttgtgcgccgatttttaaagggattcagtccttaaattataaaaa
aaaaagtctcttttaacctttaattctggaaatgcaacatttcttgaaaatgttataatccttctctaaaggggccagtgagaacacattatcagcatgt
actatacccaaacaggcatttttatctttacctatgcaaaactgacaacacacaaactgacgttcttttcttcttactggcaaatccgttgctactttaa
agcaggtagaccaaagtcctgtggatattaatgcaggcgccgatgggcttcccagttaaggacacattagcccattttatattttaaggtagtttccctt
taaaaaaaaaaaaaaggtttgactcctagagaaaaagattttgcattgtaagacacagtgcagacattggtcataatagtagcgatccactgtattgtta
ttttaatttcaatgtttaatggagcaacctgactctagtgttgggttacattttattttgtttttatatttccatggagctgcagaagaatcacatttgg
tttaagtgctgtttcattttaggtttgtatttggctttgctcccaatctgcagcacctctcacatctgtagtttgattggcaggactgggaggcaaacta
ggaggatgtcacaatgggcaaggctgcttgctagatctgttgtgaaaatggtaaaacctcaagacaggatttctattttcggctgcatcagttattgcat
gtaaccaacctaatgcaacttatagtagcaacaaaattaccgacatgccgtgcccagcgctgcttgtgatccctctgtgaattcttgccttcataggaca
attagatattgctgttagacatactctagtagcagtagtaaataggtgatttgtctttgctgcatttataggaaagggaggcttaaactgcagaaggcaa
ttggttgcgcttgtcctttttgctttttacaggtctttttatttgattaacagaaatattttattctttaaggtttttgtaccgttgtggatcatagttg
ggagattttgatgggttatgggtacagcgcaatggccctgaagaagtgcatgtacaaatcattggttctgtttgatggtttgtacataagctgggagttg
taaatcagcatcatccgaaaaataggattttgagctcagaaactaaggttctgattcattataatcttgttggcgaggaagaagcagcactgtccattta
tacctactagagaacgagacagttgaatgactgcttatataaacagcctatagcactggtgggctttctgagctgatgcggtgggctctgagcacgaaag
ggacacaggttcctcatagttatacttggctgctgttgtttgttggcttatccccactgtttttgttggcatagtaaatatattggtgctgagtgagata
ttgtttctccatatgtggtcagaaactaaactgcacatacgtaggggtgaaggaggcggtaaattgggtgccctcaggttgagtggtagaatttctgctt
cctaagaaactttggcagcaagtgacaagatctaaatggtttatgaaggtccctaattattttgcagttattcctgtttttttatgtagttggtggacta
tggccctacttgtgaactctgttttcgcccactgttgagaataccagcaccccttatcaaagggtgaaaattgtgtcctcccccttgatacatatgcatc
aaaaagatcctatacatgtgaaaatatgctgtagagagctccgttttcacccgtttggaaatatgccctgccttctcaaagttgtttagaaagggtggaa
atagagttcacaccagttcaatagaggggaactgcacagcgctctcttcacttttataagatgtgtagaggcatattcatcaaaaggtggactgtattgt
caccctttgacaaatatgcccgtaagtgtgctggtattgttagcagagaagtatagcatgcacacactatagatttcaatgctgagtgtcaggatatgag
gagctagcccaagagtttcaagctatagtggagatgagttgtggtgggggcagtttctagctcctgtggtagctgtatagggaggtacaaagtgcctctg
ttttctgtttaccagcattgtcggatcacacatccatctgacatacctttagtgcccaatccctactatgttctgccacagatgtttcttatacataact
atccacctcccctgctgttgcaggaacaaaattgaatagaacttttctttaaaaaatgtttaattcaatatggcacttactttaaagggtaactaaaccc
ttgcacttatgtcttctctacagcatgtattaatatagcagtgcctgcccagaggtgcagcaacaccatggcataatgctgactgccatctaaacatacc
cccttgtacatggctgattcttcatctctcagccacagaagtatttactaagaggacatgattgttttgtgctttgtccttttataatcagcagtacaag
caggggttctttttaggcaactgtattgtatttacctttaatgttggactcctcctccaaaccaccctgacaaggctgggcaataaatttaatataaatg
tgacatctctgccgaacacacctcaaggacacttggggtacaaatccaggtttacttaatgctggttatatattttttaagcccaagaatgtgatatgaa
tcttgtatttgacggctttcaggtctcttgttattcagctctacttttgccactagtgaccagttaacactcagcagcagatgaaagggctggttcacct
tcaagctaacttttggtatgttatagaacttttcaattgtcttcattttttgttcctgaactatttaccttcatcttttgcctcttttcagttctccaat
gcgggtcacggacccctgcagcgaaaaaaactattgctattttataggtattgccaatttttattgcttatcttgctatgcagtccccttactattcatt
tagtggttcatttaagcaactgcccagttgttaaggtatattggaccctagcaaccagatagctgcagaaatagcaaatttcaaattgtctcagaatatt
actacatcataataaaagttcagttataggaaaactaacctgttaaagaaatggccacggatcggcaagtgtatttttgcacttcttataatcaagatgg
gcctttaccttaaagggcaccctgaaattgcttcccttgccagaggtgcgggctaatagagcctacaatccggttggaggaaacaacagttaaaaaaaaa
aacaagaaaactccctacagcactaggctgcccgcaccagccagtagggagctcctggggcaggcgtccatgttgtgacacagacattcgcttatctagc
aagtgccacaacccgatcattaagcgcatgtgtgtactgtaggtgggtgagttgcatccacgcatggagctgtactcattatgtacatggctgtctgcac
cgggagctccctcctggttggaacgggcggcctagcactggtgggggctgttttaaatatatatatatatatatatatatatatatatatatatataatt
gtttccctctactggagtgtgggctctattagccctggtggggggaagcaatttcattgtgataggtgcccttgaatagggggtgaatgcatgtcaggag
cattgtaaagacaataaaatgtatgcagctgtgaatgcatttcatggagggccaatttactgtgtttaaaatgcactcgagctatattacaagcacccat
gtgtatgtaaccaaaaaagctggactgcaccttatgtgagtcattggtaatgtgcaaccccaactgggtggctgtaggttgccaatgtcctcatttaaaa
aaaaaaaaaaaa
5' EST: CX900559 [+]cgatgagccgctggcatttagagtggaagagaaagcgaagacggagcgggcagaaggcggggtgcagagaggagccggctggctgcagctggattgtaag
ccgcgcacaagccttgttcctcaagaggaagtgaagccctgcaccctgttgtgttctgactggcctctcgccttctcctatcaggctcaggatgtccgca
gacaccaacagcagctctggcctgatcctcaccgctactcccagctctacatcccttgtgactagcgtgcagccaacctccagtactattttcaacagca
ctaacatcaccataccccatgtctgcttggtcattctctttgaagacattgggaattccagggtcagatactgggatctcatgctgctaatccccaatgt
tctcttttttgtgtttctgctttggaaacttccatcagcaagggccaaaatccgtgtgacatccagccccatttttaccaccttctacatactggtattt
gtggtggctgtggtgggaatcacacgggccattgtttccatgactgtcagttcttccagtgcagctacagtcactgacaaggtcctttgggaaatcacca
ggtttttcctacttgcaatagagctcagtgtggtgatcctgggcattgcttttggtcaccttgaaagtaaatccagcgtgaagagggttctagcaatcac
tgcagtctcatctcttgcttactctgtaactcagggcacacttgagatcctgtaccccgatgcacatctgtctgctgaagatttcaacatatatgggcat
ggtgggcggcatttctggcttgccagttcctgctttttc
3' EST: CX900558 [+]ttttttttttttttttaaatgaggacattggcaacctacagccacccagttggggttgcacattaccaatgactcacataaggtgcagtccagctttttt
ggttacatacacatgggtgcttgtaatatagctcgagtgcattttaaacacagtaaattggccctccatgaaatgcattcacagctgcatacattttatt
gtctttacaatgctcctgacatgcattcaccccctattcaagggcacctatcacaatgaaattgcttccccccaccagggctaatagagcccacactcca
gtagagggaaacaattatatatatatatatatatatatatatatatatatatatatttaaaacagcccccaccagtgctaggccgcccgttccaaccagg
agggagctcccggtgcagacagccatgtacataatgagtacagctccatgcgtggatgcaactcacccacctacagtacacacatgcgcttaatgatcgg
gttgtggcacttgctagataagcgaatgtctgtgtcacaacatggacgcctgccccaggagctccctactggctggtgcgggcagcctagtgctgtaggg
agttttcttgtttttttttttaactgttgtttcctccaaccggattgtaggctctattagcccgcacctctggcaagggaagcaatttcagggtgccctt
taaggtaaaggcccatcttgattataagaagtgcaaaaatacacttgccgatccgtggccatttctttaacaggttagttttcctataactgaactttta
ttatgatgtagtaat
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: