XB-CLONE-3260773
Clone Name: IMAGE:7683487
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Gene:
clasp1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC128634 [+]
CGCGCTTGCGCAGTGGCGCTTCTATTGAGCAGTAAGAGCAGCCGGGGCGGGGAGTCGGAGCCAGACGGGAGGGACAGCAGCGATGCGCCGGGGCCTGGAG
CGGCGGAACAGCCGGCAGCCTTAGTGTGAGAAGCGGCCTCCCCGGCACCCACACAGCACAGCCTCCCCCAGGGCACGGAGCAGAACTATGTACCTCTGGA
CCAGTGACTTAGGGTTACAGTGCCTCTCGCCCTGCTCGCTGCAGGTTTAACTTGGCCTTTTTATATCCGGGCAGCTGCTCGGCAGACACATGCCGGATTG
TTCTCATTCCCAGGATTGTATCTCTGAAGGCCGAACCCTCGTCAGCTTTTTGGCAACAAGAGGAGCCCGGCTTGGCGCATTCACTCGAATCATTGGTGAC
CTTGTGCCCCCTACGGTGCCCCCTTTCCTAGGCCCAGAGCTATGGAGCAGGGAATGGACTACTGGCTGGGGCAGATACAGCAGAAGGATGTGGGCAAGAG
GCTGCAGGTTGGACCGGACCTGATGGAATACCTGTCGGACAGACAGAAGTCCATAGACCTGGAGCAGGACCAGACTGTGCTGGATAGAATGGTCGATGGA
CTTGCCACTTCCTGGGTGAATTCTAGTAATTATAAGGTGGCCCTGCTGGGCATGGACATTCTGTCGGCGCTGGTGACCCGACTGCAGGATCGATTCAGAT
CACAGATTGGCACAGTGCTGCCCAGTTTAATGGACCGGTTAGGGGATGCCAAGGACTCGGTGCGGGAGCAGGACCAGAACCTGCTGATAAAGATAATGGA
ACAAGCCTCCAACCCACAGTACGTGTGGGAACGGATGTTTTCGGGATTCAAACACAAGAATTTCCGCACGCGGGAAGGAGTCTGCCTCTGCCTTATTGCC
ACCCTCAATGTGTACGGAGCCCACAGTCTGACACTCAGTAAGATTGTACCGCATATCTGTAACCTACTGGGGGATCCCAATAGCCAGGTCCGAGATGCTG
CAATAAACTGCCTCGTAGAGATCTACAGACACGTAGGGGAACGCGTCCGAGCGGATCTCAGCAAGAAAGGGCTGCCCCAATCACGACTGAATGTCATTTT
CACCAAATTCGATGAGGTGCAGAAATCGGGGACCATGATTCTCTCCTCTGCCGATAAAAACTTTGATGATGAAGATTCCGTCGATGGCAACCGACCCTCA
TCGGCCAGTTCTTCTGCCTCCTCTAAAGCCCCTCAAGCTGCCCGCAGGGGGGTGAGTCTGGGCACCGCCCGCCGGCCTGGAACCTCGAGTGCCGCGCCCA
AGCCGGGAGGAACCGCCAAGGAGGGAGCGGGGGGAGTGGATGAGGAGGATTTTATCCGAGGGTTTGAAGATGTTCCGACTGTCCAGATCTACTCCAGCCG
AGACCTGGAAGAATCTCTGAACAAGATCCGCGAGATCCTCTCTGATGATAAACACGACTGGGAGCAGCGAATCAGCGCTCTGAAGAAGATCCGGTCGTTG
CTGTTAGCGGGGGCAGCAGAATATGACACTTTCTTCCCGCAGCTCCGCCTCCTCGATGGAGCCTTTAAACTGTCGGCCAAGGACTTGCGCTCCCAGGTTG
TGCGGGAGGCCTGTATCACCCTGGGGCACCTCTCCTCAGTGCTGGGGAACAAGTTTGACCATGGGGCAGAAGCGATCATGCCAACGGTCTTCAACCTGGT
CCCCAACAGTGCCAAAATCATGGCCACCTCCGGCGTCGTAGCCATCCGGCTAATCATACGTCAAACGCACGTCCCAAGGTTGATCCCCATCATCACCAGT
AACTGCACCTCCAAGTCCGTCGCCGTTAGAAGGCGCTGCTATGAGTTTTTGGATCTGCTGCTACAGGAATGGCAAACGCACTCGCTGGAAAGGCATGTAT
CGGTGTTAGCGGAGACCATCAAGAAGGGGATACATGATGCGGATTCAGAAGCAAGGATCGTCGCAAGGAAGTGCTACTGGGGGTTCCACAGCCATTTCAG
TAAGGAGGCAGAGCAGCTGTTCCACACGTTGGAGTCTTCCTACCAGAAAGCCCTGCAGTCCCATCTGAAGAACTCGGACAGTATCGTGTCTCTGCCCCAG
TCGGACCGGTCCTCCTCCAGCTCCCAGGAGAGCCTCAACCGCCCACTCTCGGCCAAAAGAAGCCCCACGGGCAGCACTGTGTCTAGAGCCTCAACAGCTA
CTTCCAAATCCACCCCCGGCTCCCTGCAGCGCTCCCGTAGCGACATCGATGTGAATGCCGCCACCTGCGCTAAATCCAAGGCAACGTCCGGAGCCTCCGC
TGCCCCCTTCAGCTCTGTCGCCGCCCTGCCGCCCGGATCCTACGCCTCCCTGGGTCGCATTCGCACCAGAAGACAGAGTTCCGGCAGCACCACCAGCACG
GCCTCTACCCCAGCAGACACCCGCGGGCGCAGCCGAGCCAAAGTCGTGTCGCAGTCACAGCCCGGCAGCCGGTCCAGCTCACCAGGGAAGCTCCTGGGAA
GTGGCTATGGGGGGATAGCGAGCGGCCCCCAACGGGTCCCCCAGATGCCCTCCTCTGAGAAGAGAAGCAAGATCCCCCGCAGCCAGGGGTGCAGCAGGGA
GACCAGCCCAAGCAGGATTGGCTTAGATCGCTTTGGGATCAGCCAACCAGGGAGGATCCCCTCGGCAATGCGCGTCCTGAGTTCCAGCACAGACCTGGAG
GCGGCCGTGGCAGATGCGCTGAAGAAGCCGGTGCGCCGGCGCTATGAGCCCTACGGAATGTACTCGGATGACGATGCCAACAGCGACGCGTCGAGCGCCT
GCTCGGAGAGGTCCTACAGCTCCAAGAATGGCGGCATCCCGCACTACCTGCGCCAAACGGAGGATGTGGCCGAGGTGCTGAATCACTGCGCCAGCTCCAA
TTGGTCTGAGAGGAAGGAGGGGCTCATTGGGCTGCAGAACCTGCTCAAGAGCCAAAGGACACTCAGCCGCGTGGAACTGAAACGGCTCTGCGAGATCTTC
ACCCGGATGTTTGCGGATCCTCACAGCAAGAGAGTGTTCAGCATGTTCCTGGAGACCCTCGTTGACTTTGTCATAATTCACAAGGACGACCTGCAGGACT
GGCTCTTCATCCTCCTCACTCAGTTACTAAAGAAAATGGGCGCCGACCTGCTGGGCTCCGTGCAGGCCAAAGTGCAGAAGGCGCTGGATGTGACCAGGGA
TTCCTTCCCCTTTGACCAACAGTTCAACATCCTGATGAGATTCATTGTGGATCAGACCCAGACTCCCAACCTCAAGGTGAAAGTCGCGATACTGAAGTAC
ATCGAGTCCCTCGCCCGACAGATGGACCCAACGGACTTTGTCAACTCCAGTGAGACCCGCCTGGCTGTGTCCAGAATCATCACTTGGACGACTGAGCCGA
AGAGTTCTGATGTCAGGAAGGCGGCACAGGTGGTCTTAATCTCTCTGTTTGAGCTGAACACCCCCGAGTTCACCATGTTACTGGGGGCCCTGCCCAAAAC
CTTCCAGGATGGGGCCACAAAGCTCCTGCATAACCACCTGAAGAACTCCAGCAACAGCAGCATGGGATCCCCGAGCAACACCATTGGCCGAACTCCTTCC
AGGCACTCGAGCAGCCGCGCCAGCCCCCTCACATCTCCCACCAATTGCTCCCACGGTGGCCTCTCCCCCAGCATGCTGGATTACGACACTGAAAACCTGA
ATTCGGATGAGATTTACAGCTCCCTGCGGGGGGTGACGGAGGCCATCGAGAAGTTCAGTTTCCGCAGCCAAGTGGATCTCAATGAGCCTGTGAGGCGGGA
GGGCAAGAAGGAGAGTGAGCTGGGCTCCTGTGATGTGGGTATCGCCTCTCCGGCCAGCGATCTCCGGGGGGGCACAGACATGGTGGAAGGGGGACGGATG
GCGCTGGATAACAAGACCTCTCTGCTGAACACCCAGCCGCCCCGGGCATTCACTGGCCCCCGCGGGCGCGAGTACAACCCCTACGCCTACTCCGACTCCA
TTAACAGCTACGACAAAACGGCGCTAAAGGAGGCGGTGTTTGACGACGACATGGACCAGCTGCGCGACGTGCCCATAGACCACTCAGACCTGGTGGCCGA
CCTCCTGAAGGAACTGTCCAATCACAACGAGCGGGTGGAGGAGCGCAAGGGGGCCCTCTGTGAGCTGCTGAAGATCACGAGGGAAGACAGCCTGGCCGTA
TGGGAGGAACATTTCAAGACCATTCTGCTGCTCCTGCTGGAAACACTGGGGGACAAAGATCATGCCATTCGCGCCTTGGCACTGCGGGTACTGAGAGAGA
TCCTGCGGAACCAGCCGGCCCGGTTTAAGAATTACGCGGAGCTGACCATTATGAAGACGCTGGAAGCCCATAAAGACTCACATAAAGAGGTCGTCAGAGC
CGCAGAGGAAGCAGCATCAACCCTGGCCGGGTCCATCCACCCCGAGCAGTGCATCAAGGTCCTGTGCCCCATCATCCAAACTGCCGACTACCCCATCAAC
CTCGCTGCCATCAAAATGCAAACGAAAGTCATTGAGAGGATTTCCAAGGAGTCTCTACACCAGATCCTCCCGGATATCATTCCAGGCTTGTTACAGGGCT
ACGATAACACAGAGAGCAGCGTCCGCAAAGCGAGCGTCTTCTGCCTGGTCGCCATCTACTCTGTGATTGGAGAAGAGCTGAAACCGTACCTGGCGCAACT
GACTGGCGGTAAGATGAAGCTGTTGAACCTGTATATAAAGAGAGCGCAGACGACAAACAGTAACAGCAGCTCCTCGTCGGATGTCTCGACGCACAGTTAG
GGGAGGGGCCGACGCCGGATTCGCTTCCCAGATTGGCGGAGAGGAGGGAGCGGTTGCACCTCCCACGCGCACAGGTTTCGCTCAGCGATTCGCCATCGCA
TCCCTTTAACACTAAAGAAAATTCTCACCTTTAAAAAAAAAAAAAAA
5' EST: CX901263 [+]cgcgcttgcgcagtggcgcttctattgagcagtaagagcagccggggcggggagtcggagccagacgggagggacagcagcgatgcgccggggcctggag
cggcggaacagccggcagccttagtgtgagaagcggcctccccggcacccacacagcacagcctcccccagggcacggagcagaactatgtacctctgga
ccagtgacttagggttacagtgcctctcgccctgctcgctgcaggtttaacttggcctttttatatccgggcagctgctcggcagacacatgccggattg
ttctcattcccaggattgtatctctgaaggccgaaccctcgtcagctttttggcaacaagaggagcccggcttggcgcattcactcgaatcattggtgac
cttgtgccccctacggtgccccctttcctaggcccagagctatggagcagggaatggactactggctggggcagatacagcagaaggatgtgggcaagag
gctgcaggttggaccggacctgatggaatacctgtcggacagacagaagtccatagacctggagcaggaccagactgtgctggatagaatggtcgatgga
cttgccacttcctgggtgaattctagtaattataaggtggccctgctgggcatggacattctgtcggcgctggtgacccgactgcaggatcgattcagat
cacagattggcacagtgctgcccagtttaatggaccggttaggggatgccaaggactcggtgcgggagcaggaccagaacctgctgataaagataatgga
acaagcctccacccacagtacgtgtgggaacggatgttttcgggatcaaacacaagaatttcgcacgcggg
3' EST: CX901262 [+]tttttttttttttttaaaggtgagaattttctttagtgttaaagggatgcgatggcgaatcgctgagcgaaacctgtgcgcgtgggaggtgcaaccgctc
cctcctctccgccaatctgggaagcgaatccggcgtcggcccctcccctaactgtgcgtcgagacatccgacgaggagctgctgttactgtttgtcgtct
gcgctctctttatatacaggttcaacagcttcatcttaccgccagtcagttgcgccaggtacggtttcagctcttctccaatcacagagtagatggcgac
caggcagaagacgctcgctttgcggacgctgctctctgtgttatcgtagccctgtaacaagcctggaatgatatccgggaggatctggtgtagagactcc
ttggaaatcctctcaatgactttcgtttgcattttgatggcagcgaggttgatggggtagtcggcagtttggatgatggggcacaggaccttgatgcact
gctcggggtggatggacccggccagggttgatgctgcttcctctgcggctctgacgacctctttatgtgagtctttatgggcttccagcgtcttcataat
ggtcagctccgcgtaattcttaaaccgggccggctggttccgcaggatctctctcagtacccgcagtgccaaggcgcgaatggcatgatctttgtccccc
agtgtttccagcaggagcagcagaatggtcttgaaatgttcctcccatacggccaggctgtcttccctcgtgatcttcagcagctcacagagggccccct
tgcgctcctccacccgctcgttgtgattggacag
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: