XB-CLONE-3259266
Clone Name: IMAGE:7684630
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Gene:
rlf
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC167714 [+]
gggagagtcccgaacggggctagccagcaaagacacaccgcctttatcgctttccatggctcgaacccaccgcttggcagaagacctgagggagctagag
gacacgctgaaagaggacgatgtgtctgcaccctcctcttctgtgtactgcagtcgcttctgcgagatactaatgcagcatactgaagaaaatacagctt
cagaaaacttaagtcattgtgcaggtgtttatggaactgctttgcagagctttgctactgctcgcccatacctcactactgaatgtgaagatgttttgct
tctactcggaaggcttttgttaagctgttttgaagtaatactttcaatgaccgaagatgatctctcatgcaattatggattgcaattaaagaaatctatt
atggattcacataacatactagtggaatttggaaataacaatttacagttattgattgatatggtccagaatggaggagcttggaaaaaccatgttctag
ttaaaatattgtctcaacaacctgttgagcctgaagaagtccagaaatggatttcacaagaggggtcctgttttctacagatgcgaattaagcacttaat
gaaatcaaactgcatacagcaagcaatgatcttatcgaagatttgttcagacacagctgaattgctgaatgatttttttttcgtcagtcatttatcacat
gcctgtgtactatgttacctaatgaggatgcttttaaagagatttcaaagatggatggaaaggaaatattagatacaatatgtaatctggaatctgaagg
acaagataatacagcatttattctctgcactacttatctcacccagcaactgcagaatgaaatcacttcttgctcctgggagttgactctattttggagc
aagctgcagagaaggatcgatccatgtttaaacacatttcttgaaagatgcaggcagtttggagtcattgctaaaacacaacaacatctttttttcctta
tcagagttgtgcatgcagaggctggagatgacggtgttccggtttcaatagtgttatgcataagagcccttcagaatccttcaaatgacactgatgcaac
aaagacttctgtttgcaaaacaatagcttgcctcttaccacaagatttggaggtgagacgagcctgccaactcacagaatttttatttgagccaactttt
gatagtctaaatatattggaggagctatttcagcagcctgatcaaaaaaatgatgaagaatcaagtgttatttcaaactccctacgctgtgaacttttgc
tcgctttaaagagtcactggatgtttgatcctgagttctgggactggaaaacattaaagcggcattgtctaaaacttctagggaaagaagaatctgatca
ggaggaagagaattttgataatcctttagttaatgaggcagatgttttaaacccttcattaggtagctatgaggactacattgaaaaagcacaagcatct
ccagaatatgttcctaatgaacttgaaactaccaaagtgaaaaagccagttggatcatctgaaaggtataaaaggtggcttcaatataaattttactgct
taatctgcaacagagaagtaattgaggccagaattctccatcattctaagatgcactttgctgatggcatatacacttgtcctgtttgtattaagaagtt
caagaagaaggatatttttgtacctcatgtaatggaccatatgaaaatgcccatgcgacatcgaccaaggaaggacaaaatgcaggaactgaaacccgat
atggactctgctgaagatgcatgtgatactatgggttacatttcatttaaaacaatacaagataaacaacttcaagacagagatgtttacccatgcccag
gcactggttgttctagagtgttcaagcaatttaagtacttaagcatacatcttaaagctgagcatcacaatagtgatgataatgcaaaacactatttgga
tatgaaaaacttgagagagaaatgtgccttttgcagaagacattttattactggtttccatttaaagcagcacatgagaattcatttagcatcacagcct
ttcttatgtgcttcaatagactgcagtgcaagatttaatactattaatgaacttctcattcacaaacagtcacaccctgacccccaatacaagtgtgaat
tagaaggctgcagcttagtttttggtgacctgggtctcctttatcaccatgaagctcaacattttagagatgcttcatatgtatgcagtttttcaggttg
caaaaagttctattattctagaactgagcacctagaacacattgtaacgcacacaataagttctaataatggtaaagatgtaattaatgaaataacctgc
aagcaagaaaaccaacacctttcttcccttaataatcaagcttacacactcagtaatttggacaaactttgtggcaatagccccatgctccaggatacaa
atatagtacctgaaaagaagattccaacagagcctggagatgttagttgtaaacaaatacatttcaatgcttcatctcataatccatgtgaacatttaca
cacattaacagactgtttaaaaaatgcaggaggcttgcactgcacttgcaaaatagcaaatacagttaaagtcagtctccaaaggattaaccctcttcag
ttttcacagagcttagctgggcataatacagttaatatcacagacaaaaaacacaatgagtctgctggagaggacacaccaaaaactcctttattgccta
ataccgaacagttaaactgtgtaaacaagtgtgtaccaagctcagacaaaacaggttctgcagaagattgtggtgagacacatttatcctctgtatgcca
tagatcaacatgtgagaacaccataaatgaacttctgacaagtctgaagcatttaaacctaaagaactctaattcatgtataaccacctctggtgcccaa
gattctgaagcaaatgccagctcatctattgattgtgctgttcctaaaaactctcttccactgaaacaagaaaaagtgcacagtcaatatctcactcagc
tagctagtaaaccatacctttgtgaacttaaaggatgcaaatttgcttttgccacgaaagatgctcttttagtgcattatgtaaaaaaacatcattacac
aagggaaagaactcttaagttgcaaatatttcaaaataaatatactccatttcaatgccatatctgccaaacagcattttcaaggagaacgctccttcga
attcattacaaaaaagtacatcacctatcaaaagagagagccacctgccgaagaggcagaagaaagctggaaaatattcaaactcatattaatgagaggc
ataacaattatattcagttccaagcaaatgaagccaacacatcgactcatgacagtaaatctctcatccaagattttaataatgaaaccttcactgattc
gttgtctgatgaaacagatgctggaaatgaaaatggcacatctggaagtcattcagacaattttaaaggtggggaagggagaggtagaagacgtgttgtg
gcccaaggaaaattgtgttatatattgaataaataccacaaaccatttcactgtatccataatacctgtaatgcctcttttacatcccagaaaagcttag
ttcgtcactaccaactggtccatcaatataacaaagaatctttatgtctagaaagagataaagtcgatactagaagggagtatggcaaatgtagacgaat
atatacttgtaaatataaggcatgtagaaaaagtttcatttgtgcaaaagcactttccaagcactacacagaatttcacaaccatgaaaatgaagaaaaa
gaacttgatgacacatatgcagagaacagctctaaaccccaaacggatgaagagaaatccagtgatgaaacagaaactgatgagtctgaaatttattgcg
atgcagaaggctgcactgcagttttttcagatcacgcaagctacactcgacacattttatcccgtcacaggagatatagactgtatgaaggacggagaaa
aagaagactaaaactggaacaagatgaaatgcagggaaaatatattgcatcaaggcacggaagtaatttgatgtataatagaaaaaaacggagagtgcga
aaaaaggaagacaaaagggagtcggctgaattaaaatcaagagaagaagccttacaaatgtgtgcgcagaatgttgatatcactcagttcccatgcatga
tccatggttgttcttcagttgtaaaattagaaagtagtataattcggcattacaagctaacacataacctgtcagcgaattatgttaccgataatactgc
aggacttgtatactgtgtcaagaacttcccacactgtaaaacagaacaaaacttttcaactgaagctcatagtccagagaggcacgattcaattaaagaa
cataaaatacctaaattacatttacatggtgatatccacagtaaatttgatcaaagtgatggagaagagggggttcagattaataagtcagacttgtcta
gttcagatgagagagactctctaaatcaagctaaaatgttgcatggttgtttaaggcaatctggtattcattcatctcttaaagaatgcatcagttctgg
tgctaaggtgccctcttttgctaattttcaaacatgtgacgtgggtaacaatggaccacacaattctgtggatgcagactcattaaaatcccaaaatgtg
gacagtgaaatacagttaacatcaaatttaaaggcaagctttaagccaaatggatttgaatcatcatttctgaaatttttacaagaaactagggattcgg
atgatgatgataagttagaggatacagaatggaaaccgcaacagcactgcaaattgcagaattctttccaaactaaaaaactctcttttagagaaactag
tagaaatcaccataaggtatgcttatcccaaggcaagcgcttaggacatgatgatctaccaggattccagcctttgttgtccagtaatgtacagactgca
acatcaatacctaccttacaaactctaaggaccatattggacaaagcattaacagaacgtggagatcttgctttaaaacaactccattaccaacgacccg
ttgttgttctggagcgatcaaaatttaatatccccctcatagacttgttctcaagtaaaaaaactgatgaactgtgtgtggggatttcatgagaactaaa
ccaaaaagtgtgccagtattaatttgaggaatccatctgaaatgcacagaaggattattggtgaaaaaaagacttgtattatagcaattttgtgcaatta
gatgtatcatttcctgaatgtattttatctgtgttcacaaatatacatttgtttatcttcccagatgaaaacattttatttggtgctaggacatcaatat
aattgggcttcctttggaaatacatgtgcatgattgtcctgggatttgaataatacaagtttttcatacttgaatgaaatatgcacttgtgtgctttctg
taatatattgtacagtatgacctcttatatatttttttgagttgtgtttcaataaattgcgaactttcaaaaaaaaaaaaaaaa
5' EST: CX903950 [+]ggagagtcccgaacggggctagccagcaaagacacaccgcctttatcgctttccatggctcgaacccaccgcttggcagaagacctgagggagctagagg
acacgctgaaagaggacgatgtgtctgcaccctcctcttctgtgtactgcagtcgcttctgcgagatactaatgcagcatactgaagaaaatacagcttc
agaaaacttaagtcattgtgcaggtgtttatggaactgctttgcagagctttgctactgctcgcccatacctcactactgaatgtgaagatgttttgctt
ctactcggaaggcttttgttaagctgttttgaagtaatactttcaatgaccgaagatgatctctcatgcaattatggattgcaattaaagaaatctatta
tggattcacataacatactagtggaatttggaaataacaatttacagttattgattgatatggtccagaatggaggagcttggaaaaaccatgttctagt
taaaatattgtctcaacaacctgttgagcctgaagaagtccagaaatggatttcacaagaggggtcctgttttctacagatgcgaattaagcacttaatg
aaatcaaactgcatacagcaagcaatgatcttatcgaagatttgttcagacacagctgaattgctgaatgatttttttttcgtcagtcatttatcacatg
cctgtgtactatgttacctaatgaggatgcttttaaagagatttcaaagatggatggaaaggaaatattagatacaatatgtaatctggaatctgaagga
tcagataatacagcatttattctctgcactacttatctcacccagcaactgcagaatgaaatcacttcttgct
3' EST: CX903949 [+]ttttttttttttttttgaaagttcgcaatttattgaaacacaactcaaaaaaatatataagaggtcatactgtacaatatattacagaaagcacacaagt
gcatatttcattcaagtatgaaaaacttgtattattcaaatcccaggacaatcatgcacatgtatttccaaaggaagcccaattatattgatgtcctagc
accaaataaaatgttttcatctgggaagataaacaaatgtatatttgtgaacacagataaaatacattcaggaaatgatacatctaattgcacaaaattg
ctataatacaagtctttttttcaccaataatccttctgtgcatttcagatggattcctcaaattaatactggcacactttttggtttagttctcatgaaa
tccccacacacagttcatcagtttttttacttgagaacaagtctatgagggggatattaaattttgatcgctccagaacaacaacgggtcgttggtaatg
gagttgttttaaagcaagatctccacgttctgttaatgctttgtccaatatggtccttagagtttgtaaggtaggtattgatgttgcagtctgtacatta
ctggacaacaaaggctggaatcctggtagatcatcatgtcctaagcgcttgccttgggataagcataccttatggtgatttctactagtttctctaaaag
agagttttttagtttggaaagaattctgcaatttgcagtgctgttgcggtttccattctgtatcctctaacttatcatcatcatccgaatccctagtttc
t
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: