XB-CLONE-3258928
Clone Name: IMAGE:7685086
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Gene:
eif4g3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC168018 [+]
ggcggacgcagcgccatgttggatgctccgctttctgagtgaggaaaatccgccggctctgagcatccccgaagcgacagcgacagccgcccccccgggc
cgtgtccctcccccatgaggatcgggtcttgagccccctgctgcctccctattgcccctgtgccctggggggccccgctctgccatgagtctcccgcaga
aagcggccctcaagcccgccgcaccgaccggcaccggagccctagtggttgggcctccccccagtgccgcccctcccgccgcagccccctccgccatccg
ggcccttcaaccgccgcctcaaatatccaggggagccctgcaacagagcctcgatgagcgaatcttctccccctccgccgtttctgccgtctacagcaca
gttgcagcacaggtagccaggcaacccggccctccggccccttctccttattctactcatgatttatccaagggacaccctaatctggctgccacccctc
cagggcatgccgcctccccaggattgtcacaggcttcctatgcgtcgggccagagcccagcagcacccaccctggtgtaccctcagccaccgcagactat
gagtactcagccccaaaatcgctcaccatttgcagctggaccccgacctgcccatcaccagggaggattcagacccatccagtttttccagaggacccag
attcagcctccccgtgccgccatccagactagtagcccctccatccgggccggggcccagaccccaactgcggtctatcaaaccaaccagcacatcatga
tggtcaatcatctgccgatgccatatgcaatgccccaggggccacagtactgcattccccagtaccgacacagtgcccccccatacgtggggcctcccca
gcagtacccagttcagccgcctggtcctagtccgttctaccccggcccggggcccggagactttccgaccccctatggagccccgttctactcgagccaa
cccgtgtaccagtctgcgcccatcattgtgcctacccagcagcagcagcagccgcctccccctgccaagcgagagaagaagccgattagaatacgagacc
ccaatcaagggggcaaagatatcacagaggaaatcatgtcgggagtaggcagtcggaactccaccccgcccatcctcagacccaactctacgccgacgcc
ccctcagcagctgcctagccaggcgcccgagcacggcccagtggtgtacgggactatggagagtcctcatctcccagcgactttccctgccgctcctgtc
agcgaacccaaaccagaagagaagcccaagccggagcacaccgtaaaagccacgccccccccatgtgtgcggcccgaccccgtagatattggcaccagtg
atgtcatcgagcagacgcccagacccgcctcctctgagcgccccgagctccaaccgtgtcttctgccgggggtaacggccgcccctatcgtggtacccat
tgtgcccaccgcccaagcctttccttgccacctggatgatacgaggggcagcgagagcccggcagaggtgcgtgcccccactcccagcaccccgcaagag
tcagacccggccgaaacggaggaggtggtatgcaaggaccctgtggggcaagtggcgcccgctgatgcccctctgcctgcctctgctttgcctgctcagg
aaatgaatggcctaggggacgatatggccggtacggcggccggcgctgcaggggaggagtcggccctggcagaagtggatttgcccgaggtgcccggttt
ggctgcgccaatggatgcagacgctgaatgcactacttcttccaacgtgcctctttcctccccggctctgtctgcctccccactgcccgactctcctcct
cctctcattgctgctagcctctccccccctgccagcgccctcgccaccctctgtgcccaagatgtggcagagcaggaagatggcagagccgccaccctag
agagtgacggagagggacagggcaaagggagaaccgagggggactccgagctggaatccagtgttaaactcctgagcatcaactccaagaagagccctgt
ggaagccccggccgccataactgcaccaaagatgtggcagaaaccaaaagatgagagtccagacatagaggagatctcacagtcagagaccgagccacaa
tatgaggatgatcccctagaggacaaagtcacagaattggatcaagacccagtcacatacagcggtaccagcacaatggaggtggagcagctgtacctta
acaatgccaagccacaggagcagaatggggaggtggagcctatacgcaatggggctgatgccgtctctgagagtgaaggcaccgatatcccatcaggcac
tgaggaaggcttggcctacccttacagtaaagcaggaatcggtgcagccgagcaaccccccgacccctctcaggagcccccggcacctccacagtcggct
gatgggaagcgccagtacgaccgtgacttcctgctggggttccagtacatgcccgcgtgtgtccagaagcccgagggcctcccccccatcagcgacgtgg
ttctggacaagatcaatcagcccaaactgcccattcgggccctggatgctcggatgctgcagcgggggccagatttcacccctgcctatgctgattttgg
gagacccgcccctgggggccggggcatgccgctgctgagtgtgggcccccggcgctcccagccgggacagaggcgagagccccgaaagatcatcatgaac
atctccgtcagcgacgacgtccatctgaagaaatcggaaaacgcctggaagcccagcgtcaaacgggatagtctgccggaggatccggatagcatcagaa
ctcaggccctgttccgcaaggtccggagcattctcaacaagctcacccctcagatgttcaaccaactgatgaaacaggtgacggatctgacggtggacac
agaggagaggctcaaaggggtcattgacttggtctttgaaaaagcaatagatgagcccagtttctccgtggcctatgccaacatgtgccgctgccttgcc
acgctgaaagtgccgatggccgacaagccggggagcacggtgaatttccgtaaactgctgctcaaccgctgccagaaggagtttgagaaagataaagctg
acgacgatgttttcgagaagaagcaaaaagatctagaactggcaacaacgccagaggagaagtctcggctacaggacgaactggaagagtctaaagacaa
agcccggcgccggtccattgggaacatcaagtttatcggagaactattcaagctgaagatgctgacagaagccattatgcacgactgtgtggtcaagctg
cttaagaaccatgatgaggaatcccttgagtgcttgtgccggctactcaccaccatcggcaaggatctggactttgagaaagccaaaccacgtatggatc
agtactttaaccagatggagaagatcgttaaggagagaaaaacctcatctagaatccgctttatgctgcaggatgtcatagacctccggctgagtaactg
ggtgtcccgccgggcagaccaggggcccaagaccatcgagcagatccataaagaggccaagatagaggaacaggaggagcagaggaaagtgcagcagtta
atgaccaaagagaagaggagaccagcaggggtgcagagagtggatgaaggcggatggaacactgtgcagggggcaaagaataacagggtgctggaccctt
ccaagtttctgaaaatcactaagcccactatagatgagaagatccagttggttcccaaagctcagcctggcagctggttaaaggggagcagcggaggtgc
caaggctagtgagactgaatctctgagaccgggagcccccagcctgaacaggttctctgctctgcagcagccgctctcctctgtgtcttcctcctcctcc
tcatcgtccccctctgagctggactctcgcaggttactaaccagccgtggaagcacggggcgagagaagaacgacaaaccctctgcgccctcctccatcc
cgtctgcgccccgacccggcaccctcctgcgcggcagcagcaaagactcgggcgacaatcaggaagagctgcgccgggagacgctccagacggtcaaaca
gctgacgaacgtcgccgaacccgacaagaacactgtgccagagagacggcccaaagagccagccaagtccgagccgtcggaaccagacaaacccaccctg
tcagaggaggagatggagaggaaatccaaggccatcattgatgagtttctccatattaatgactacaaggaggccatgcagtgcgtcgaggagctcggtg
ccaatgggccgctctcagtcttcgtacgacagggggtggagtcaacgctggaacggagtcagatcaccagggatcacatgggtcagctcctctaccagct
ggtccagtctggcaaactcagcaaactggacttcttcactgggttctcggaaacgctggagctggccgacgacatggcgatcgacatcccgcacatctgg
ctgtacctggcggaactggtaacccccatgttgaaagaaggcgggatctctctacgagagctgataacagagttcggcaaagctctgctccctgtgggac
gcggaggggaattattgtctgaaatattgcaccttctatgcaaacaaatgagccataagcgagtagccgcgctttggagagagaccggccttcggtggag
agacctcttacctgaaggagaagacgtccagagctttgtcagcgagaagaacttggagttcacgctgtcggacagctcgagtccctcggagaatctctcc
aagaaggagatgtcggcagaagagctgcacaagaggctcgagcaactcattatccaggagaaggctaacgacgagcagatatttgattgggtagaggcca
gcctagatgagagccagatgagttcgcccacattccttagagctttaatgacggcggtgtgcaaagcggcaattttaggggactgttcttcctgccgcgt
agacatcaccttcctcaagcagagggttccggtcctgctaaagtatctggactcggacacagaaagagaactacaagcactttatgcactacaagcattg
atagtaaaacttgatcagcctcccaatgtgttgcgcatgtttttcgattgcctctatgacgaggaggtgatttcggaggacgctttctataagtgggaga
gcagtaaggacccggcagaacaagagggcaagggggtagcactaaagtcggtcacagccttcttcacttggctccaggaggcagaggaggagtcggagga
caattaggaagttccatccaatgagagcccgtcgcttgccccccggagacgccgccaacgccgagcagtttcagcctctttatatctttagcttttgggt
ttttctctcttttgttccagttttgccagtcagggtacagccaattggattcccacagggggggtgggcgtggctattgggaaaggtgccccaaccagtg
ttaagatgggggagatgtgaccccccaaacggccgaggggggactgaccccctttcccacattctatttattagagtcgatattttctgttttaaaactt
cccattttattttaaagaacccccaaacccagagactcgccccaaaactgccgatcattggaaagagaaataatttattcctttacagacgggtggcgcc
agtgacccactaaccccctaacgtaggaactgggggggcaggaaaccccctcccttgctgtgtagcctgccctctccccttttgggggtccctgcccatt
ttagtgtcacattttggggggcagcggctacggactgggctgcaggttcacccgcagtctcatttgtgggcactgctgtaaagctgagcagccggggagc
ctgcagatgtaccccaatgttggagggggccccctgccctgcactctgtcgccccctgccattgccccgggtatgtacagatgaggggggagaattagac
tcgcgggggggggttcccaggtgtagcccaccttcagcgctgcattcaagggaggggggcaaatggacttccaatctcgctgtggccctaacgcgcagaa
taaagagctggaactggctacggactggcacagagcgggcaccacagcgggcaccttgggactgacggtgtctcggagaagctgccggagctttccaagc
aattggcaaacgagactgtgagatgcttttctcttacattgtatttctattcattttagcctggtttcctatggaaaagaaggctaataaagtaacctgt
accctgaaaaaaaaaaaaaaa
5' EST: CX904494 [+]gcggacgcagcgccatgttggatgctccgctttctgagtgaggaaaatccgccggctctgagcatccccgaagcgacagcgacagccgcccccccgggcc
gtgtccctcccccatgaggatcgggtcttgagccccctgctgcctccctattgcccctgtgccctggggggccccgctctgccatgagtctcccgcagaa
agcggccctcaagcccgccgcaccgaccggcaccggagccctagtggttgggcctccccccagtgccgcccctcccgccgcagccccctccgccatccgg
gcccttcaaccgccgcctcaaatatccaggggagccctgcaacagagcctcgatgagcgaatcttctccccctccgccgtttctgccgtctacagcacag
ttgcagcacaggtagccaggcaacccggccctccggccccttctccttattctactcatgatttatccaagggacaccctaatctggctgccacccctcc
agggcatgccgcctccccaggattgtcacaggcttcctatgcgtcgggccagagcccagcagcacccaccctggtgtaccctcagccaccgcagactatg
agtactcagccccaaaatcgctcaccatttgcagctggaccccgacctgcccatcaccagggaggattcagacccatccagtttttccagaggacccaga
ttcagcctccccgtgccgccatccagactagtagccccctcatccgggccggggcccagaccccaactgcggtctatcaaaccaccagcacatcatgatg
3' EST: CX904493 [+]tttttttttttttttcagggtacaggttactttattagccttcttttccataggaaaccaggctaaaatgaatagaaatacaatgtaagagaaaagcatc
tcacagtctcgtttgccaattgcttggaaagctccggcagcttctccgagacaccgtcagtcccaaggtgcccgctgtggtgcccgctctgtgccagtcc
gtagccagttccagctctttattctgcgcgttagggccacagcgagattggaagtccatttgcccccctcccttgaatgcagcgctgaaggtgggctaca
cctgggaacccccccccgcgagtctaattctcccccctcatctgtacatacccggggcaatggcagggggcgacagagtgcagggcagggggccccctcc
aacattggggtacatctgcaggctccccggctgctcagctttacagcagtgcccacaaatgagactgcgggtgaacctgcagcccagtccgtagccgctg
ccccccaaaatgtgacactaaaatgggcagggacccccaaaaggggagagggcaggctacacagcaagggagggggtttcctgcccccccagttcctacg
ttagggggttagtgggtcactggcgccacccgtctgtaaaggaataaattatttctctttccaatgatcggcagttttggggcgagtctctgggtttggg
ggttctttaaaataaaatgggaagttttaaaacagaaaatatcgactctaataaatagaatgtg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: