XB-CLONE-3258175
Clone Name: IMAGE:7685666
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Gene:
fam117b
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC133276
[+]
CTCTTCTCCGTCCCCTGTGGCCCTCTCGTCAGTTGGCAGGCCTGGGTGGCAGCAAAGGGCCTCCCCAGAGAGGAAGAGTCCTAGCTCCCCTAAAGGGGAT
AAAACACAACCTCCTTCATTAAGTCCATCAAGTTTAATAAGGCGCACATCTTCACTTGATACACTTGCTGCTCCTTATCTATCCGGTCATTGGCCTCGTG
ATAATCAAGTGCAGACAGCTCCTTGTATGAAAGACCAGTCCACCCAGACGGAGAGTGCTTGGGCTGAAGAATATGCAGAGAAGAAAAAAGGGTCTCATAA
GCGCTCTGCATCCTGGGGAAGCAATGAGCAGCTAAAAGAGATTGCAAAATTACGTCAACAACTTCAGAGAAGTAAACACAGCAGCAGACACCATCGAGAA
AAAGACAGGCAGTCTCCATTTCATGGCAACCACTCTGCAATTAACCAGAGTTCGATGTCCAAAAATGTACTTACTCCAGTGACTATTCCCATTACAAAAT
CCTCTGGGTCTCGATTTCGCAACAGTGTAGAGGGGCTGAACCAAGAGATTGAGATAATTACAAAAGAAAATGGAGAGAAGGAAGAGCAACTCTTGCCCCA
AGATATCCCAGATGGACATCGTGCTCCCCCACCTCTAGCTCTGCGCAGTATAGATACTCAGACACCTGGAGGGGTTGACCGCGGAAGCAACAATAGCAGC
CGCTCACAATCCGTGTCACCAGCCTCTTTCATTACAATCTCTAATGAGTGCAACGAGGAAAGCCCTTGCCCAGCTGATGACAATATTGCAGATCCTCGAG
ACAAAGAGAATGGAAATCATTCCCCTCTTCCCAAGTATGCTACATCCCCAAAACCAAACAACAGTTACATGTTCAAGAGAGAACCGCCTGAGGGTTGTGA
AAGGGTGAAAGTATTTGAGGAAGGCGCGCCAAAGCCACTGCATGAAATTCCACCATTTTACTGTCCTGATAAGAACAAAGTGAATTTCATTCCAAAGAGC
GGTTCTGCTTTTTGTCTGGTCAGCATTCTCAAGCCTCTTCTACCCAATCAGGAAATAACCTTCAAAGGCTCCACCCACAGTCTTACAGTGCCTTCTGGAA
TACCATCTTCTCTTGTTCAAAACCTCAGCATGACATCAATATCAGGAAACATAGAGCAGGAACGCAATTCTCGAGGGACAAATACCCTTGCTCCCCCACT
CTCTCTGCTACACCCATCAGGCCAAATTGATGAAGAAGAAGACTAAAAACCATGTTGGCTGTTCATTGTTACAGAGGAACTGCACTGCTCTGGTAAAACA
AGTGACTTCAAATGCAGTATTTTCATAAGCTGTTAAAAATATTGACCGTATTCTCCCTTAGTAAATTGGGCAGCAAGCAGACTTGCTGGCTTTGGCATCT
GTAATGGATATAATTGTCAAGATCTGGAAAGGACTTAACGTCAGCTAATTCTTTTACCTTGGCTTTCCTGTATTGAACATAAATCTTGTGTTCTGATTTA
ATTAAAATAATCTGGTGCCTCTATCTGCAAAGTGTATCAGCCACATAGACACTCTCATCCTTAAGTTCTGGGCTTTGTATCACTATCACTTTCCTAAGCA
GAACAAGACAAACTGCTTTCACAAACAGATGGTCTGTTTTCCAGATAAGTCCATGTTAACAGTAGTTACCACTCAGAAATGGAAACAAGAAGGCATTTTT
GAAAAGGTATAAACTAATGGCCAGGATGTTTAATAAAGTTTTAAGCTGTTTTCCAGCTGCAGGGTAAATGATGTACCTACTAATCTCATGTTAAGATAAC
TGATCTTAACATGATAAGTAAACCTGTAAATAGAAAGGTTAAAGGGGAGATGAGTAGCAGACTGGTGGGTATTGTGTGGTTTGTTAAATGAAATTATATG
TACCTCCTGCTTCATCATTTTATTATAGTTGGCAATATTGTGGTATATGTGTCCTACTTCTTTAGCTGACATTTGGATTATATTAAACATTGTAAGAGGG
TTCCAGCTGAAACTTATATTTGCTAGGCAAGGTATCTGTTAATCTGTACCACTCATAAAATGTTTCCTTACCTTTGCATATCATTTTTTCCTGTCTTTTG
GATCCTTTCTTCCCACACTTGCAGTAAATCTTCCAATGCATTTGAGTTGTACCTGACTGTTTAGTCTAAAATATAAAGCACAGACTATCCAATGCCTTCA
TCTACCACTGTTTAATTCCTTTGATGGCAACTTCAATAATTAAAGTATTTTTTTTCATAGCTGCTGAAGAAATATTTTTAGAATCTGTGTAGTGTTAAAC
CTGGCTCCCTCTAAGTTATATTGATAGTGTGGTAGTGAATTGCAGAGGCTCATAGAGTTTTTCTGGTATTTACCCACTGTAATTAACATGATAGCTTATC
TAAATTATAGATAAAAGATAAATGTTATTTGTAGCCATCAAATTTCAAGACACAGCAACATTATGAATTGGACTTCTGCTACTATCAAGTTATGCAATGT
GGAAGTCTGCTAAATGTAAATTTTTCCTCCACCTTATGACAAACCTGAATTATAGCTAAGGTCAGACTTGGGACTCATTGCCAGACCACACACACTGGAG
ATAATCAGCTGAGACAAGTGCCAGAGAATCAGGGCCCATGCTGTTGTATATTATGGTAATGTCACATAACCTGCCAATGTGGCCCCAGCCGTTCATTGTT
AGGAGACTGCGATCTTCAAAAGCTGAATATATCGGGTCAAAAGGAGGACTTAAAGCATCAGATATTGTAATTTTGACTATATATATATATATATATATAT
ATATATATATATATATATATATATATATATATATATATATATAATTATATATATATATATATATATATATAAATATACATACTTTTGTGTAAATAATACA
ACATGGGAGGTTTTGTATAACCAATTTTTAGCTTAGTGCTACTATTTCTTTAAAAGGGATAAAGGCTAATAATAGTCTGAGCAACCAGAACAGAGCAGCA
CTCGTACTGACAGCTGCCCTCTTATCTTGACTGACTAAATTGTGCCATTAGGTGGACTGCACCATAGGGTCTATCTGTGCAAAAAGACACTCTTACTCTG
CTTTATTCAGGGTAAAGAAAAACTATGGCTGGTACTGTTATATCTGTTCTATTGGATAGCTGTACCTGTAATATCCCTTTTGTTAGGCCTCTGGGCCAAG
TACTGATAATGTCTTTATGTTTGTGTATTGCAGAAATTTCCAAGTAGTCCGATCCCAAAGATAGCTGCTATAAAGCTGTTAAATGTGAGATTTTCCAGAT
CAAGAAATCAGTTTGTTACCAGAAATACAGTGTAAACCAATAAAACCTGTTTGACTCCAACATATTCATGATTCAAGCTGCATGTATGATCTGGCATATC
TTGGTTTTTCAAGGTAGGGTACCTAATAGCCTCACCCTTCCGATTAGGCATTTAAAATAATGGCTGTTATCGTTTCTATAGCAATAACTCTGACTTGAGT
GTATTGTTCCATCAGCCTTATTTAAAGTAGAGTGTAAATGTTTCTACAGTGCTAAACCAGAATGCAATGAACAGATTTGTGTTAAGGTCAAAATGTACCT
AGAAATGGTATGTGAATGTTGGATTTGTTAATAAAAAAAATTATAACGGGGAAACAAAAAAAAAAAAAAA
5' EST: CX905897
[+]ctcttctccgtcccctgtggccctctcgtcagttggcaggcctgggtggcagcaaagggcctccccagagaggaagagtcctagctcccctaaaggggat
aaaacacaacctccttcattaagtccatcaagtttaataaggcgcacatcttcacttgatacacttgctgctccttatctatccggtcattggcctcgtg
ataatcaagtgcagacagctccttgtatgaaagaccagtccacccagacggagagtgcttgggctgaagaatatgcagagaagaaaaaagggtctcataa
gcgctctgcatcctggggaagcaatgagcagctaaaagagattgcaaaattacgtcaacaacttcagagaagtaaacacagcagcagacaccatcgagaa
aaagacaggcagtctccatttcatggcaaccactctgcaattaaccagagttcgatgtccaaaaatgtacttactccagtgactattcccattacaaaat
cctctgggtctcgatttcgcaacagtgtagaggggctgaaccaagagattgagataattacaaaagaaaatggagagaaggaagagcaactcttgcccca
agatatcccagatggacatcgtgctcccccacctctagctctgcgcagtatagatactcagacacctggaggggttgaccgcggaagcaacaatagcagc
cgctcacaatccgtgtcaccagcctctttcattacaatctctaatgagtgcaacgaggaaagcccttgcccagctgatgacaatattgcagatcctcgag
acaaagagaatggaaatcattcccctcttcccaagtatgctacatccccaaaaccaaacaacagtta
3' EST: CX905896
[+]ccccgttataattttttttattaacaaatccaacattcacataccatttctaggtacattttgaccttaacacaaatctgttcattgcattctggtttag
cactgtagaaacatttacactctactttaaataaggctgatggaacaatacactcaagtcagagttattgctatagaaacgataacagccattattttaa
atgcctaatcggaagggtgaggctattaggtaccctaccttgaaaaaccaagatatgccagatcatacatgcagcttgaatcatgaatatgttggagtca
aacaggttttattggtttacactgtatttctggtaacaaactgatttcttgatctggaaaatctcacatttaacagctttatagcagctatctttgggat
cggactacttggaaatttctgcaatacacaaacataaagacattatcagtacttggcccagaggcctaacaaaagggatattacaggtacagctatccaa
tagaacagatataacagtaccagccatagtttttctttaccctgaataaagcagagtaagagtgtctttttgcacagatagaccctatggtgcagtccac
ctaatggcacaatttagtcagtcaagataagagggcagctgtcagtacgagtgctgctctgttctggttgctcagactattattagcctttatccctttt
aaagaaatagtagcactaagctaaaaattggttatacaaaacctcccatgttgtattattta
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41414.jpg) |
Usage:
Suppliers: