XB-CLONE-3257971
Clone Name: IMAGE:7686199
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Gene:
pcf11
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC167611 [+]
gacttgcgaagggagcggagggaagctggtgaaggtgctgcggagtgccatgcggtgcgaagggcctatggattaagctctaggctggatgtagccctgt
gcactctcggctgtatatttatataatttattagttcttgttgttagcctcctttccggagagacaagatgtcggcggaagacgaagccagggaagacgc
gcgtcgggattaccagtcgtccctggaagacctgacgtttaacagcaagccgcacatcaacatgctgaccatcctggcggaggagaatgtgcagttcgcc
aaggatatcgtcattcttatcgaagcgcaaatcgccaaggctcgagcaaccgagaagcttccagtcatgtacctaatggattccattgttaaaaacgtcg
ggagagactatctcgccgcctttgctaaaaacctagttaccacctttatttgtgtgtttgaaaaggtggatgaaaacaccaggaaaagtttatataagtt
acggtctacatgggatgaactatttccttcaaagaaactgtatgcacttgatgtgagagtcagccaaatagatcctgcctggcccgttaagcctctgccc
ccaaatgtgaatacttctagtatccatgtgaatcctaaattcctaaacaaaccagaggaaagtcccccagctaaagcgtcagcgccaagccctgcgccac
cccgtgctgctaactctgaggcacaaaagcatctgtcacaagagcagctaataaggcaacaattgctggtgaagcagaaacagttattagaacttcaaca
aaagaagttggagctcgagctagaacagacaagagagcagctggcaacttcactttcttcttcaaaaccagttgtctcgccaaaaattccacacatgcca
gtcaaggccccaccgcctgtttctgaaaaaagcaaagttcccgctcctacccagcgcattgagaaatcccatgaaaagatgccagtgccaaccagagatc
ctcgtcttgttaaaggaaatcagcattcagcgcacgggaaagatcaagggcacaaaaaggagatgaagtccaacatggcctgtatgccagacctaaagcc
tattaaacctctgttgaatgataaagcaaatgcttctgcaaagcaagaaaaggcaaagtctaatgaaaagctacctaagcgtgaattggtgcaatctgag
cttaaagaccgatcaaagtcaccatcacctttaaaagctaaattgtctcatatgagggataaatctcaaggcaccgagaatgtaagggacttagatataa
gtaggagagacccaaggctgcacaagcacctccgtaataaatctgacgacaaagatgctgagcccaaggagaagcggaaatgtgacaaaaaagaggaaca
taaatccgagcacagagtagcgggaaaccgaaacaaacctgtcaatgggattgtgccaaaaaacgaaaccagtacaaactatccagataaaacaaaacct
ggaagaatcggcaatagaaaaaggtcacggtcaccgaagagccgatcaccagcccattcacctaagggtagagataggaggtcacccaaggcacggccga
gaagtatatcccctgttcattccctcccaaaaattggcagattgcgtcagcctggtagtaagcaatctcacactgaggactttaacgcaagctctcggga
agagcggattaagaggaacgcgaaaacagatgcacgagacggcagaagacaaaagagactgtcggaggagaggccacaggatcctgtatctttgatatcc
aataagtctaatactgagccaaaagaggatatagaaaattggcaagggcctaaagccaacaagcgatggaagagtggatgggaggacaataaaaactctc
agaatgacgatcatcagggtaaccggcccccttatcagaggcataatagttggcaaaacagcactaaaggggttgcttctccccgagctccaaagcagca
gcattggttaagtgttgatgctgatctgaaaatacctaaagaattaacaagtgcaagcaaagcagagttgctgaaaaaggcaaatgaacgcttttccttg
ggagaaatcacacacgatgagcttcttgtagtggtacatcagatccgtcagctcttccagtaccaagaagaaaaacagagaggaagtgtgtgggatagcc
ccactgaggaaaataagggtgccttaaggaagaaacctctgctttccaatccagaaacaacctattatgaacataaagcaaagctgaaaaggacacaagt
tcagcatgcatttgctgacagagaatatatggatccagaatatttggacatggagctggatgaaatggcgcactctggcagagatggtgaacttagtggg
agagcggaagacaaaatgcctgaaagaaatgagcagtttcctgaaagacaaaggctttcccccctacttgggactagacatccagatgaggatgcacatg
atggctacagaaggcatgaagatcaaagagaactttccaaaggtttacgagaagaaccgagacctttgtttcctgaacgattcccacgctcagtccccat
gtacgacgaatctgaggaggcaccttatgaagaaaatgcagactctagaatgagtggaaacaaagctaatccaaagcttcctttgatggaagagagaaga
ccactgcttgatatcccaccactcatccctgttaacagaggagatgcccctatcttgaaaagttttgaagagccttccaatgttgcaaacgttagggaaa
gttctccagtaaatgcatgcatgccatttgatggtgcatcaggaaatcaggttggacctcagcgcttcgaagcagtctcaggacagccagtcgatgccca
ttttgatagtatgggtgccaaccaagtcgagaacacaaggtttgatggcatgatgggccagcctaaaaatcaccagggtccacaaggtatgcagaaatat
gacggagcgcaaaaccaacctatagtgtcaagccagcctgtgggagttaaaagattagagggccctcccggtcagccaatgaatgcagcaagatttgaag
gcccaccaagtcaacaattaggagcgccaagatttgaaggggccgcagggcaaccagtgggccctcaacgatttgagggaatgcagcaattaggcccaca
gagatttgaaggagtggcaggtccacaaagatttgatggctctcctagacctctaatgcagcaaaggtttgacggaattccttgtcagcctatgaggttt
gaacagccaatgagatttgaggcatctcaaggacaacagttaggcccagtgaggtttgaaggaccggttggtccaagatttgaagtaaatcagcctcagc
ggtttgacagtggacctggtccccaacccttaatgaggtttgatgggccaatggggcagatgaggcccaggtttgatggaacacctggcaatagatttga
taatcaacgacctcatatgcaaaggtttgatatgcctggacaaccctgccagagatttgaaggcccaggtccagggcatcaaactccacctcggtttgat
gggccaatgcaggtccaaccacgctttgatggtcctttaccacagaggtttgatggcccaaatcagcaacccagatttgaactttcaatgaaccatcaag
gaccacgctttgataatattcatccacccgttaggcaaaacgctccaccttttggacaaagtggtccttatggggaacctcagaaccatttccatggaca
gccacaaaatatgccaccacaaggcctgccccctcaggggatgctgccacaaggcctgccccatcaagggatgcagccgcagggcttgctgccacaaggg
atgctgccaaatggtatgcagccgcagggcatgctgccacaagggatgccgccacaaggaattccacctcagggaatgcagcaacaaggaatgccacttc
agcggcctgagcaaagatttgatttgccacagggaccaaattttgccggaccttctggtcctggggtgcagtgttttccaaatcaacttcctagaccatc
tgggccttatttcgatgataagaatccaaaaccccaaggaccccagtttgcaaactttaataaccttcctgtaggaaacttagcaccaagccaaccattt
ggaccattaggaggaatgtctcagcctgcaggaatatctcaacccactgcaatatctcaaccttccggaatgtctcagccggcattttccagtcaagatc
agaactatgtccccccactggggtctgcaggaggagtgtttgtacagccaggtggttcgtttccgtttcctgaaaatcacttgggacagcttgatgttaa
tgagttattctcaaagctactttcaacgggaatcattcaaaaagttacaccgaatgaaacctcttctactcaagcaactgaatcttctgcacccgctgca
gcggaggaagaggaggaggatgcagatggagatggagatgaagatcaagatgtcccagaccttaccacttttcaaatggaagacctaaaacagcgatatg
acagtgttataaaccgactctatactggcatccagtgctattcctgtggcatgaggtttaccaaatcccaaactgatatatacgcagatcatctggactg
gcattatcgtcaaaacaggacagagaaggatgttagcaggaagataacccaccggcgctggtactatagcttaaaggactggatagagtttgaggagatt
gctgacttggaggagcgggctaaaagtcagttctttgagaaggtgcatgaggaagtcatattaaaaactcaagaagctgctaaggaaaaggaatttctca
gtgtgccagctgggcccgcgggggcagatgagatatgtgaaatttgccaagaacagtttgaacagtactgggacgaggaggaggaagagtggcatctgaa
aaatgcaatgcgcatgaatgataagatttaccatccatcatgctatgaggactataaaaatacatcgtccttcttggatagcacaccatcccccagcaag
actcttcttgaaaatccactcaatgccatgctaaatctggtcaaagaggaggtaacggaacctacatgctctgtgaaagaggaacccgccgattacaccg
catcaagctgctgcgagatacctctgctcacggaaatcaaaaaagaacctgctgaacctgagtaaaaaaaaaaaaaaa
5' EST: CX906291 [+]cttgcgaagggagcggagggaagctggtgaaggtgctgcggagtgccatgcggtgcgaagggcctatggattaagctctaggctggatgtagccctgtgc
actctcggctgtatatttatataatttattagttcttgttgttagcctcctttccggagagacaagatgtcggcggaagacgaagccagggaagacgcgc
gtcgggattaccagtcgtccctggaagacctgacgtttaacagcaagccgcacatcaacatgctgaccatcctggcggaggagaatgtgcagttcgccaa
ggatatcgtcattcttatcgaagcgcaaatcgccaaggctcgagcaaccgagaagcttccagtcatgtacctaatggattccattgttaaaaacgtcggg
agagactatctcgccgcctttgctaaaaacctagttaccacctttatttgtgtgtttgaaaaggtggatgaaaacaccaggaaaagtttatataagttac
ggtctacatgggatgaactatttccttcaaagaaactgtatgcacttgatgtgagagtcagccaaatagatcctgcctggcccgttaagcctctgccccc
aaatgtgaatacttctagtatccatgtgaatcctaaattcctaaacaaaccagaggaaagtcccccagctaaagcgtcagcgccaagccctgcgccaccc
cgtgctgctaactctgaggcacaaaagcatctgtcacaagagcagctaataaggcaacaattgctggtgaagcagaaacagttattagaactttcacaaa
agaagttggagctcgagctagaac
3' EST: CX906290 [+]tttttttttttttttactcaggttcagcaggttcttttttgatttccgtgagcagaggtatctcgcagcagcttgatgcggtgtaatcggcgggttcctc
tttcacagagcatgtaggttccgttacctcctctttgaccagatttagcatggcattgagtggattttcaagaagagtcttgctgggggatggtgtgcta
tccaagaaggacgatgtatttttatagtcctcatagcatgatggatggtaaatcttatcattcatgcgcattgcatttttcagatgccactcttcctcct
cctcgtcccagtactgttcaaactgttcttggcaaatttcacatatctcatctgcccccgcgggcccagctggcacactgagaaattccttttccttagc
agcttcttgagtttttaatatgacttcctcatgcaccttctcaaagaactgacttttagcccgctcctccaagtcagcaatctcctcaaactctatccag
tcctttaagctatagtaccagcgccggtgggttatcttcctgctaacatccttctctgtcctgttttgacgataatgccagtccagatgatctgcgtata
tatcagtttgggatttggtaaacctcatgccacaggaatagcactggatgccagtatagagtcggtttataacactgtcatatcgctgttttaggtcttc
catttgaaaagtggtaaggtctgggacatcttgatcttcatctccatctccatctgcatcctcctcctcttcctccgctgcagcgggtgcag
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: