XB-CLONE-3257873
Clone Name: IMAGE:7686030
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Gene:
efcab6
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC166350 [+]
ggaattgcccccgggacaggataggatcatgtcttcttcaagcttcagggatttgcgcccccaaacccgggggatcacctttgccggacgaccgcaatct
gccttgagccgatccttgtcaaggagcagccgctcatcagtccaatcttctcttgtctcctcggtgaagtcggtccgagaccccaccctcaccccccttg
aaatagaggggatcatctaccggagggttaatgagaacgaagaggatgtgacgaatgctttcctgacgctggacacggagcagaacttcaccgttacacg
aggggaattctacagggtgcttcagaatttcatcctacccctgacagagtcccagtttaatgagttattagaaaagattcccgtcaataacaacggcaca
atcccgtatctggatttcctggcaaagttcctgaggacttctagcagcgccacctgtgcgaggagcgtaaagaggagttggagctgctcccaagccaatc
aggtgctgagtctgaatgaactggagatccgtttgaaaaacacggtctcaaagaacctgaagaacgtggtccggtcctgtcggctcttcgattacaacca
gaacgggcagatccagaagcaggagctgcgccggatcctggagaactgctgctttaagatgaagacggcagaatttgaaaagctatggaatcggtactgt
ataggcaaaaagaacacactcgactacagagaactcttgcgaaatctcggcgttaatgtggagcttcacaacaagccaacggcagagaatgttgcaagcg
ctctaaactgggaggccgttcaacgggagcaacaaaaagaaaagaactgccgaccgccccctcccctgaaagagttaagcgccgaggattacaccatcga
tgacatagaagcggcgctccggaaaaagatttcggcttcgtaccccaacctggtgaaggctttccgggcgtttgatggatcccactccggctacgtgtcg
gctgaggccttacagtcagtcattaataactttatcttcccactgcccaatgggacctttcagaagttaatgaacagattggccattaaatgcacagaca
ggattggctgggagcagttcctgaggagattccaagggccggagagtatggcaaacgggcagaccatcccgatgaagccgaatcatagagttaatccggt
gaggggggaaagcccagtggtttccagtgaacacatccttcagaagctccagaggcatttccaggacgcctacccttccctgaaacaggcctttctggta
ctggatgaggggcgtaaaggaaggatcagcaggaaggagctgcgccgaattgtggactgcatgatgtttcgggtcacagacgctcagttcaaggagctta
tgatcatcctcgatccacagcacacggggttcatcagttacctgcagttcctcgacttgtttgaagataaagagtctgtttcaggccataaatggctcca
taacatgaggacccccctaaaggagacgcctgtaacgctggagtgggaaaagatggggaacatcctctgcgagaagattgaacacaactgggccgacttc
tccaaagcaatgaagtcatgtgacagcgccggatgcgggatggtggacaaggatcagtttaaaagaatcttgcagtcattctgcccccctctgtctgaca
ggcactataacatgctgtgtgagcagtacaatgatggagccaatggatatatctcctacatggattttctgcgaaatgtgggagtttgcacttccgccgc
tggtgactttaatggtgtgagtactgagattgtacatggaagtcagttaagagaggagctgagacaggatgatctttctgccaggatgaaggagattgaa
agccaggccacacagctagtgaagaagatgccagtggaggaggtaatcgggaagctgaaggactgtgtgatcgggcaggaattctcccccagggaaagct
ttctggccttcgccaagcagcacaaggggcgcatcagcaagaaggacttcaggaaagttctacgggagcacgagatccatttagacgaagatcagtttaa
gcggctcaccgagaaactgggattcacaaaagaagagctcagttacttggacttcgtttctttattcgaagaccccactggaaacggaccaggagccgct
ctagatactggatcgaaccatcacgtgaacgacactaagttccactacatgtcggcagaggattgtctggctcagctccgggataaactgcaacagggat
acggggatctgtactctgccttctacaaaatagacagcaaccgcgatggtctcgttaccatgcacgatttccgccacctcctcgatagtttcatgttcat
catcaaaccgcaggagttccagcgattgctgggtctgctggggctcaatttgtcttctaccctcaactacaaggagtttctgcacctcttccagaagccg
caaagccctgtgtatccgccctggcttcagtcatcccacagcccgaagcagagtatggggggtgaagacctggcgtgtgaacaagctcattactatctgg
tcaccaaggcccaaggcagatggcacgacatggccaagacattctgtgagatcgacagtgaaggaaacggcatcgtacagaagaaagacctgcgcaacgt
cttgttcagattttaccttcccatccatcccaaggagtttgagaagctttggggccggtatgatccggaaggaaagggctttctgacccacaatgaattc
cttgagaagctcggcatcagctttgcgtccggtgacagcggcccaagcaaacagatcacggaggacaatcgggcgtgcctggaggggcattacaacaacc
agctgaaaatccaagaggagatggacgcgttccacaaacaccagaccaaggcgctggatatgaaggacatagagcagcagatcaaggataaattccggga
ttattaccatgatttcagcgcagccttcgccaagatcgatagaaacaaggacgggcagatatccgtgcatgacctgcgcgctatgctgcagagcctaaac
ttccatttggatgatgagcagtttgccaacctactgtacaggctgagaatcaaagtgacggacaacaagctgtcgtatccggatttcctgagaatgatcg
acgacggcagagcttctaagtatggccaaaggcagcgtccggtttcggccgcagagacccccccgaaactgagcctgcagaaggtgctgctgaaattgaa
agaagccgtggtgtcttcacacgatgccctgtataaggcattctcgtcgtttgatagggacggcacgggcaccataaaggcctcggaattgcaccaaata
ctcgacagcttctgctttaaactctcagaccggcaatttgggtaccttctcagcaagctgccgctcggcgaggagcagacaatcgactggaaagccttcc
tgcatcattttagcacatcccaccctgagccttcttcctcctgggtggagagagtccaacatgccaccaggcccagatccactcaggggctcaccatgaa
ggacattctaaaccacattcgggaggtggtcaccgctcggttctacacggtggcccaggacttcatcagtctggattacgccaacatcaacgccgtgtcc
aaggaggatttccgagagcttttcaacaaggattttatgctcctgacggatgaacagttcgagaacctgtggaacacgctgccgctcaacccctacggaa
acctgaaataccatgagtttttgagtcagtttggcggcggcgacgccctggtgtctccatatgagaagacggcaggggaccaatcggagttcatctataa
accagcgtccgctttagctgggaatcacacccccccagaatcgcaggcagggatcaggcgccccaaaactgctccggccatattgaataaacccaagact
gatatccagaggccaaagacagccggtgcttcatcaaggctggtcaactgtgagcagatagaagccagactccgggggtccctccagaaggtttggcaag
aggttctacgagcctgccgagaccacgatcccgagagaaccggggagatcaccactaacgacttctcaggggttctcaagcagtttaacgccgagaccaa
cgaggaggaactggaacgactgaccgtcaagtacgattcgaggaataacggcaagttctcctacgcggatttcttccgcaacatcatgctgggacccaag
ccgctagagaagtccccgctgcacaggatgaaagtgcagaagacaagggttcctataagtacaggaccggatgggccgtgtttcctaaatgccatgatga
gaattcagcccaaggtactggattgctggcgacccatgagaagagccttcctgtcttttgatgaagcgagaaccggctttataagtgtgcaggaattcaa
acaggttttacggaaatcggggctaaatctatcagaagacgagttctttcatattctgggattctttgacaaagacttgaaagcgaaggtctcatataac
gacttcctgagagcgttcctgcgatagatccggaaactcctggaatcctgtgggtttgactggcggatcattcgtttatgatgtcagctgattttttagt
gtgggtttgagattagattcccatgagcctctctgctgtgtatttattatgggcccctgggctggagactgattgatcacattatcattagggttatatt
gcctccaacggaacagatctggcaccggtgacccaattctgtggaataagaacccattaaagttttcaaatacacaaagaaaaaaaaaaaaaaaaaaaaa
aaaaaaaaaaaaaaaaaaaa
5' EST: CX906445 [+]gaattgcccccgggacaggataggatcatgtcttcttcaagcttcagggatttgcgcccccaaacccgggggatcacctttgccggacgaccgcaatctg
ccttgagccgatccttgtcaaggagcagccgctcatcagtccaatcttctcttgtctcctcggtgaagtcggtccgagaccccaccctcaccccccttga
aatagaggggatcatctaccggagggttaatgagaacgaagaggatgtgacgaatgctttcctgacgctggacacggagcagaacttcaccgttacacga
ggggaattctacagggtgcttcagaatttcatcctacccctgacagagtcccagtttaatgagttattagaaaagattcccgtcaataacaacggcacaa
tcccgtatctggatttcctggcaaagttcctgaggacttctagcagcgccacctgtgcgaggagcgtaaagaggagttggagctgctcccaagccaatca
ggtgctgagtctgaatgaactggagatccgtttgaaaaacacggtctcaaagaacctgaagaacgtggtccggtcctgtcggctcttcgattacaaccag
aacgggcagatccagaagcaggagctgcgccggatcctggagaactgctgctttaagatgaagacggcagaatttgaaaagctatggaatcggtactgta
taggcaaaaagaacacactcgactacagagaactcttgcgaaatctcggcgttaatgtggagcttcacaacaagccaacggcagagaatgttgcaagcgc
tctaaactgggaggccgttcaacgggagcaacaaaaagaaaagaactgc
3' EST: CX906444 [+]ggggatttgaaaactttaatgggttcttattccacagaattgggtcaccggtgccagatctgttccgttggaggcaatataaccctaatgataatgtgat
caatcagtctccagcccaggggcccataataaatacacagcagagaggctcatgggaatctaatctcaaacccacactaaaaaatcagctgacatcataa
acgaatgatccgccagtcaaacccacaggattccaggagtttccggatctatcgcaggaacgctctcaggaagtcgttatatgagaccttcgctttcaag
tctttgtcaaagaatcccagaatatgaaagaactcgtcttctgatagatttagccccgatttccgtaaaacctgtttgaattcctgcacacttataaagc
cggttctcgcttcatcaaaagacaggaaggctcttctcatgggtcgccagcaatccagtaccttgggctgaattctcatcatggcatttaggaaacacgg
cccatccggtcctgtacttataggaacccttgtcttctgcactttcatcctgtgcagcggggacttctctagcggcttgggtcccagcatgatgttgcgg
aagaaatccgcgta
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: