XB-CLONE-3257805
Clone Name: IMAGE:7686238
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Gene:
zfat
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121281 [+]
GCGCTGGGGAGTTTAGAGCCGGGAGTGAGCGGGACGTGTGTCCGGTATAGGCTGCGGGGAGGGGGCGCTGTCAGCGCTTCTGCAGTACCCGCAGCCGCCT
ATCATGTTATGTGCCCCCCTGCTGGATGGAAGCAGCTCGATCTTCATGTGCAAACTCTGCAACCTCTTTTCCCCCAATCATTCCGAGCTGGTGCGGCACG
TGTCCGACAATCACACGGAGGAGGGCCTGACCGCCGGCGACATCATTATCCCGCTGAGACCCCTCGCGGCTTCCAACAGGAGCGGTGACGATTTATCAGC
GGTAAAAAGGAAAAGAGGGAGGCCCAAAGGTTCCACCAAGAAGTCCATCGCAGAAGAGGAAAGCACAGAACAGAGCAGCAATGCATCCGGCGACCTCCTC
CCGGAAACGGACGAGAAACCGGACCCTGTACCGGAGAACCTGGAATGTCGCAAGTGCAGCAGAACCTTCTCCAATGTCCGGCAGCTGAAAAAACACATTT
GTATTATTGTGTTGAACGATGATGAAGATGTCACCTCAGGCAACGATTTTGAAGGGAAATTCATCCAGCCGGAGTCCGACAAAGAAAGGGAAAGACAACC
AAAGAGGTCCCGCGTTCAGAAAACGGACAAATCCGCCCAGCCCAAGGATTCGGATAAAGCACAAGGTGTTCACCCGATCGTCCGGGTCCTTCTAACTGCG
CACGAGGCTATCCCAGGAGCAACAAAAATCATCCCTATAGAAGCAGAAACTCCCGAAGCCGAACCAGAAATAAATCCTCAGACTTCCGAGCAAGAGCAGG
TGCAGCGCAAGGGTTACCAGGAATACGCCATTCAGCAGATACCCACCGAGCTGGCAACCAAAGCCAACAGGGTAGGACCGACCCAGCTCAAGATTTTCAC
TTGCGAGTACTGCAATAAAGTCTTCAAGTTCAAGCACTCCTTGCAAGCTCACTTGAGGATCCACACTAAGGAGAAACCATACAAATGCTCGCTGTGCAGC
TACGCCAGTGCGATCAAAGCCAACCTGAGCGTCCATATGAGGAAACACACGGGGGAGAAGTTCAGCTGCGATCACTGCACCTTCAGCTGCCTGAGCAAGG
GCCACCTCAAAGTCCACGTAGAGCGAGTCCACAAGAAGATAAAGCAGCACTGCCGCTTCTGCAAGAAAAAGTACTCCGACGTGAAGAATCTAATCAAGCA
CATCAAGGAAACGCATGACCTGCTGGATAAAAAGGTCCAGGAGACCTTCGACGAGCTGAGGTTGCGCACCAGGGAAGGGAAGCGCCAGCTTCTGTACGAC
TGTCACATCTGTGAGCGCAAGTTCAAAAATGAGCTGGAAAGGGACAGACACATGCTGGTTCATGGCGACGAGAGACCGTTCGCTTGCGAGCTGTGCGGTC
ACGGAGCCACCAAATACCAGTCCCTCGAGCTGCACATCCGCAAGCACCCGTTTGTGTACGTCTGCTCCTTCTGTTGTAAGAAATTTGTCAGCACGGTGAG
ACTAAAAGCCCACATCAAAGAGCGCCACTCGGACGCACAGGAGGCCTTGGTATATAATGACTCCATCAATCAAAGCTTTTGTCTTCTGGAACCAGGCAGT
GATATCCAGCCTGAAGCCCTCAGAGATATAGAAAATCCTGCTAAACCATTGACATTAAATGGGGACTTATCATTGGTTCCAGAATTGCACAGCACTGGGC
AAGAGAACACCATACAGACTGATGCCCTAGGGACAACATCAGAAGGGCAACAGGAGTCCATGGGTGCTTTCCAAGTAGCAGAGAAGGAGCCAGGAACCTG
TTTACCTATTTTAGAAAATGGCGTTGACCCCAAAGCGGGGCCACTCTCCACCCTTGGGTCTGTAGAGAATGAGATTTCCTGCAAAGGTTTTGTGCCTCTC
TCGTCTGAGAACGAGAATAATCATTTCTCTTCTGAGCATGAACCTGTTGAGGGTCTCCATTCTGCACCAGCAACCAATCAAGCTGCAGATAATATTTTGG
GTTCTGCAGTGTTGGAGGAAGGGGCTAGAAATGTATCTTCCACAACTGAGCAGAGCACAGAAGTGGCAGAAGGAGGCCCAAACCCCACAACCGAGCCTTC
TAGTCATCTTCTTGCTTCGAATCCACCACCCAGCAGCCCAGGGGAAACTGCCGCATTTATGGCCATATTAAATGGCTTACATAAGAGACAGCTGAGCACT
GCCTTAATGCAGAAGATACGAAAGATCTATGGCGAACTGGAGTGTGAATACTGCGGCAAGCTGTTCTGGTACCAAGTACACTACGACATGCACGTACGGA
CTCACACGCGGGAGCATTTGTACTACTGCTCACAGTGCAGCTACTCTTCCATTACCAAAAACTGCCTGAAAAGGCACGTGATCCAGAAACACAGCAGCTT
GCTGCTAAAATGCCCGTCGGAAGGCTGCAATTACTCCAGCCCCGATAAGTACAAGCTGCAGACGCACATCAAAATACACACGGATCTGGAGAAGAAAACC
TATTCTTGCCCGTCCTGTGAGAAGTCTTTTTCGGACGATCGTCTGATTAAATCCCATATAAAAACCAACCACCCTGAGGTTCCTTCGAGCCTAATCTCAG
AGGTTCTAGGCCGCAAAGTCCAAATGAAAGGACTGATAGGAAAACGTGCGGTGAAATGTCCTTTTTGTGACTCGTATTTCATGAAAAATGGCTCTGACCT
GCAGCGGCACATATGGGCTCATGAAGGGCTAAAACCATTTAAATGTTCGATGTGCGACTACGCTACACGCAGCAAAAGTAACCTGAAGGCTCATATGAAC
CGGCACAGCACCGAGAAGACACATTTGTGCGACATGTGCGGCAAGAAGTTCAAATCCAAAGGAACCCTTAAGAGCCATAAGCTCCTTCATACATCCGATG
GCAAGCAGTTCAAGTGCACAGTCTGTGAGTTCACGGCTGTTCAAAAACGACATCTTGTGCGTCACATGGAACAACATACCTCTTTTAAGCCGTTTGGCTG
TGCCTTATGTCACTATTCCTGCAACATACCTGGATCCTTAAAGAGACATTACAATAAAAAGCATCCGGATAAGCAGTGTGTGAACGCCGGGACGGGCGAG
ATTTCCCAGGAAGCCCTGCTCCTCCAAGGGGGCATGAAGTGTCCTGTGTGTAGCTTTGTGTATGGAACGAAGTGGGAGTTCAATAGACATCTGAAGAACA
AACATGGCCTGAAGCTGGTGGAAAATGAAGGAGACGCCAAATGGGAGTCCCTTGACACGACGGAGGAAACGTGTACCCAGTACCTTCATATCGCTGACAC
AGAAACAGATGTTCAGGGAACAGAGGCCGCGGTGGCAGCTCTCCAAGACCTTCGATATACCTCAGATACCAGTGATAAGCTTGACCCCACGGCTGTGAAC
ATTCTCCAGCAAATCATTGAGCTGGGCGCGGAACCCCACGACGCGGCTGCTGTCGCCTCCGTCGTTGCCATGGCACCCGGAACCGTTACAGTGGTCGAAC
AGGTGACGGAAGAGGAGCGCACAACCAATCACGCCGTCATGATCCAGGAGGCCTTGCAGCAAGCGTCCGTGGGCCTCGGCGAGCAGCATCACTTGGTCGT
TTCGGCAGATGACGTCGAGGGGATCGAGACTGTTACAGTTTATACTCAAGGGGAAGAGGCCTCGGAGTTTATCGTTTACGTACAGGAGGCCATGCAGCCC
GTGGGAGATGGAACCATGGAACACATCTTGCAGGAGATCTGAAGAACCTCCCCTAACCGTAATGACCGACATTTGTTACAAATTTTGTATATTTCCTTGT
AAAGTCCCCTTTTTTTAAAGAAAAAAATGAATTGAGTAAATACTATTGCCATGTAACGAGACTGCCCTAGAAGGACACTTTTCATTCAAAATGTCCTTAT
TTCCAGCAATAAATGCAGAAAACATTTTATTTAGCTCAGGTTTATTCTTTATTCACATTAACTGCCACCTGTCTCCCCACTGCCGACCAACCAATCAAAC
ATTCATCTTTTAGTGCCAGGTCACATGACGTTGTTCTCCTATAAATATGAAAATAAAGCAGTTCAGCTTTGTGATCAGGGCTGGAAGTTACTCAAAAAAA
AAAAAAA
5' EST: CX906563 [+]ggggagtttagagccgggagtgagcgggacgtgtgtccggtataggctgcggggagggggcgctgtcagcgcttctgcagtacccgcagccgcctatcat
gttatgtgcccccctgctggatggaagcagctcgatcttcatgtgcaaactctgcaacctcttttcccccaatcattccgagctggtgcggcacgtgtcc
gacaatcacacggaggagggcctgaccgccggcgacatcattatcccgctgagacccctcgcggcttccaacaggagcggtgacgatttatcagcggtaa
aaaggaaaagagggaggcccaaaggttccaccaagaagtccatcgcagaagaggaaagcacagaacagagcagcaatgcatccggcgacctcctcccgga
aacggacgagaaaccggaccctgtaccggagaacctggaatgtcgcaagtgcagcagaaccttctccaatgtccggcagctgaaaaaacacatttgtatt
attgtgttgaacgatgatgaagatgtcacctcaggcaacgattttgaagggaaattcatccagccggagtccgacaaagaaagggaaagacaaccaaaga
ggtcccgcgttcagaaaacggacaaatccgcccagcccaaggattcggataaagcacaaggtgttcacccgatcgtccgggtccttctaactgcgcacga
ggctatcccaggagcaacaaaaatcatccctatagaagcagaaactcccgaagccgaaccagaaataaatcctcagacttccgagcaagagcaggtgcag
cgcaagggttaccaggaatacgccattcagcagatacccaccgagctggcaaccaagccaacagggt
3' EST: CX906562 [+]ttttttttttttttgagtaacttccagccctgatcacaaagctgaactgctttattttcatatttataggagaacaacgtcatgtgacctggcactaaaa
gatgaatgtttgattggttggtcggcagtggggagacaggtggcagttaatgtgaataaagaataaacctgagctaaataaaatgttttctgcatttatt
gctggaaataaggacattttgaatgaaaagtgtccttctagggcagtctcgttacatggcaatagtatttactcaattcatttttttctttaaaaaaagg
ggactttacaaggaaatatacaaaatttgtaacaaatgtcggtcattacggttaggggaggttcttcagatctcctgcaagatgtgttccatggttccat
ctcccacgggctgcatggcctcctgtacgtaaacgataaactccgaggcctcttccccttgagtataaactgtaacagtctcgatcccctcgacgtcatc
tgccgaaacgaccaagtgatgctgctcgccgaggcccacggacgcttgctgcaaggcctcctggatcatgacggcgtgattggttgtgcgctcctcttcc
gtcacctgttcgaccactgtaacggttccgggtgccatggcaacgacggaggcgacagcagccgcgtcgtggggttccgcgcccagctcaatgatttgct
ggagaatgttcacagccgtggggtcaagcttatcactggtatctgaggtatatcgaaggtcttggagagctgccaccgcggcctctgttccctgaacatc
tgtttctgtgtcagcgatatgaaggtactggg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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