XB-CLONE-3257787
Clone Name: IMAGE:7686386
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Gene:
slc12a6
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122930 [+]
GTTGGTGGCGCGATTGACGATATATTATCCCCTCAACAGGTTGGACTCTAAGCCGCATGTGAAGATATATTGCACTGGAATGGACAAGGATGTACAAAGT
CATTTTTCTTAACAAGAAACCATCGTGGCACAAGTATTTTGTTTGTGTTCTCCAACCAAAAAAGTTAGTTATTCAGAGTAACTAAAAAAATACATGGGTC
TATCTTACTGCATCTTCGTATAGCTGGATTAAATACACATATATTTTCTGGGATTTTACATAGAAGACAGGACGGCCGCTCAAAGCGAGGAGTTGCCACC
AATGCCACCCAAAATGGCATCGGTACGGTTCACCGTTACACCCACCAAAATGGACGAAATGGGGGCAATCTCTGACACCAGCCCAGATCTCAGTTCATCC
TCCCGTGTGCGGTTTAGCTCCAGAGAGAGTGTCAGAGATGCCAGCCGCAGTGAGGCTTACAGTGACACCTCACTGGCAACCACAGGAGTGGACCAAGCTA
GTGAAAGGACCTCCAACCCTATGGACAGTGGAGAAGATCGGAGCCAAACATCAGTAACAGAGGAGAACAGTAAGCTGCTAGACATTGACCGCCGGAAGAG
TTCAGTGCTTTATCAAAATCACACCGATGATGAAGGGGACTCTTATGACAGAAATCTGGCCCTGTTTGAGGAAGAAATGGATACTCGACCAAAAGTTTCA
TCCCTCCTTAACCGCTTGGCTAATTACACAAATCTGCCTCAGGGAGCAAAAGAACATGAAGAGGAAAGTGAGGGCAAGAAGAAGGCTGCTAAAACCCCCC
GAATGGGCACGATAATGGGGGTTTACCTCCCTTGTCTTCAGAACATTTTTGGGGTCATTTTATTCCTTCGACTCCCGTGGGTGGTGGGCACAGCAGGAGT
CTTGCATGCCTTCTGCATTGTATTCGCCTGCTGCTGCTGTACCATGCTGACGGCTATTTCTATGAGCGCCATTGCAACAAATGGGGTGGTGCCAGCTGGT
GGTGCCTATTTCATGATTTCTCGAGCCCTTGGTCCAGAGTTTGGAGGTGCTGTAGGCCTGTGCTTCTACCTTGGAACAACCTTTGCAACAGCTATGTACA
TCCTGGGGGCTATTGAGATATTCCTGGTGTACATTTCCCCCCAAGCAGTCATATTCCATGGAGAGGGGGTGGCAGAGGAATCAGCAGCAATGTTGAATAA
TATGCGTGTATATGGAACTGGATTCCTGATTATTATGTCTATTATTGTGTTTGTTGGAGTGCGATATGTAAACAAGTTGGCCTCTGTATTCCTGACCTGT
GTCATCATGTCTATTCTGGCAATTTATGCTGGGGCACTAAAATCTGCATTTTCTCCCCCGGACTTTCCGATTTGCTTGCTTGGAAACCGGACTCTGTCCA
AGCATCATTTTACCGAATGTGCCAAGATAGTGGAGAGCGACAACATGACCATTGTCACAGATCTGTGGAATCTATTCTGCCACCCATCGGATCAGCTGAA
TGGAACTTGTGATGAATATTTTGCCCATAATAATGTCACCTTAGTCCAGGGAATCCCAGGCTTAAAGAGCGGGATCATCTCTGAAAACCTGTGGAGCAAT
TACCTTCAAAAGGGAGACATAATAGAAAAGGCTTCATTGCCATCAACTGAGTATTTGGCTGTGCAGTCTCAAGAGTATGTTTTAGCTGACATTACGACTT
CTTTTACCCTGTTGGTTGGAATATTTTTCCCATCTGTAACAGGAATTATGGCTGGATCAAATCGCTCTGGAGACCTCAAGGATGCCCAGAAATCGATTCC
AATTGGAACTATTCTTGCAATTCTTACAACCTCCTTAGTCTACCTCAGTAATGTCATCCTGTTTGGAGCTTCTATAGATGGAGTTGTGCTCAGAGACAAG
TTTGGGGATGCAGTGAAAGGAACTCTTGTTGTTGGTGCGCTTTCATGGCCATCTCCTTGGGTCATTGTGATTGGATCTTTTTTCTCTACTTGTGGAGCTG
GACTTCAAAGTTTGACTGGAGCACCTCGCCTGCTCCAAGCCATTGCTAAGGATGGAATCATTCCATTTCTGAGGGTATTCGGACACGGAAAGAGTAATGG
GGAACCGACATGGGCTCTTCTTCTAACGGCAGTGATTGCTGAGCTGGGGATCCTTATTGCTTCTCTGGACTTGGTGGCACCTATCCTATCTATGTTCTTT
CTAATGTGTTACCTTTTTGTAAACTTGGCATGTGGATTACAGACCCTTCTCCATTCACCCAACTGGCGCCCTCGCTTCCATTATTATCACTGGACTTTAT
CTTTTCTTGGAATGGCACTGTGTTTAGCCCTCATGTTTATCTCTTCCTGGTATTATGCCCTAATTTCCATGGTAATTGCTGGAATGATCTACAAGTACAT
TGAATACCAAGGGGCCGAAAAAGAATGGGGAGATGGAATCCGTGGATTGTCCTTGAGTGCAGCACGTTTTGCTCTCCTGCGCCTGGAAGAGGGTCCACCT
CACACTAAGAACTGGAGACCTCAGCTACTTGTTTTGGTGAAATTGGATTCAGATCTCCATGTCTCTCAGCCTCGCCTTCTTTCCTTCGCCTCCCAGCTAA
AGGCTGGTAAAGGTCTGACCATTGTGGGTTCAGTACTTCTGGGAGACTACCTAGAGAATCATGCAGAGGCCCAGGCAGCGGAGCAGGCACTGAAACACTT
AATGGAGCAAGAAAAAGTAAAAGGTTTTTGCCAAGTGGTTGTGGCTCAGAAGCTGAAAGAGGGTCTTTCCCACCTTATACAGTCCTGTGGTCTTGGGGGA
ATGAGACACAACACTGTGATCATGAGCTGGCCAAGTAGCTGGAGACAGAGTGATGACTCACGGGCTTGGAAATCCTTCATCACTACCATTCGTGTAACCA
CAGCTGCCCGCCAAGCACTTCTGGTAGCAAAGAATGTATCCCTGTTCCCTGGCTCCCGTGAAATATTAGCAGAAGGGCACATTGATGTTTGGTGGATAGT
ACATGATGGGGGCATGCTCATGCTTCTGCCCTTCCTCCTTAAACAACACAAGGTGTGGCGGAAATGTAAGATGCGTATTTTCACTGTGGCTCAGATGGAA
GACAATAGTATTCAGATGAAAAAGGACTTGGCTACCTTCCTTTATCATTTACGTATCGCTGCTGATGTTGAAGTGGTGGAGATGCATGACAGCGATATAT
CTGCATACACATATGAACGCACACTCATGATGGAGCAGAGGTCACAAATGCTGAGACAGATGCGCCTTTCAAAAACAGATCGGGAAAAGGAGGCTCAGTT
GGTGAAGGACAGGAATTCAATGCTGCGTTTAACCAGTGTGGGCTCTGATGATGATGAAGATACAGAGGCTGTCCCAGATAGAGTGCACATGACTTGGACC
AAGGATAAGCACCATGCAGTTCGTGTTGCCCAAAGCAAACCAATGCCCAGCTTTCAGGACCTTCTCAACATACGACCGGACCAGTCGAATGTTCGGCGTA
TGCACACAGCTGTGAAACTTAATGAAGTCATTGTTAATAAATCCCATGATGCCAAATTGGTTTTGCTCAACATGCCAGGACCCCCTCGTAACCCTCAGGG
TGATGAGAATTATATGGAGTTTCTAGAGGTGCTTACTGAGGGCTTGGAACACGTGCTGTTAGTGAGAGGAGGTGGAACAGAGGTGATCACCATTTACTCA
TAGGTGTGCACAGTGCATTTCTTAGGTTGGCATTGTGGAAACCAGGAACCAACCTGAGAGAGAGTGGAAAGATGGAACTTTTGTTAATGCCAAATTATTC
CACTCCCTTGAATGGAACAAGGTGAAAATTCTCAGAAGTGTTGGCATTAAAGATCTTTTCCCGTAAATTGTAGTTTTCATAAGGGAGTCACCAAAATACC
GTTTAATGCCAAAGGGGAGTGCCATGAATGCATTTGCTGTCCAAAGAATCTATCCCAAACACCTCTGGCTGGAAAATACGGAAAAAGACTTCTAGCTCTC
ACCCAGTGACAATATTCATCTCTATGCAGCAGTGCTCCTGCCTTTTCAATTTGTAATGCTTAATGTACTACTCAGCTGAAGATATCCAGATTATTTAAGA
CATATCTTTGTACAAACAGATTAGCACCTTCTTCATTAGTAATATGCAAAGTTACTGTACGCATATAGCTCAGTTCATTTTTCGTGATCCAGATGTTCCA
AATGGGATCAAAGAGCTAGTACATGCTAAGCAAGTTAAGTGATTCTAAATACTAGCTTAAGGGAATTCACGCGTTTGTGCATATTGTGACTGGACAACTC
AGCTTTATCAGATCTCTTTATAACCCAGTGAACACCAAAAATGATCTACCACATCGGCCACAAAAACCTTGGGTGTTCTAATGACATTCCTGTAAAAGCT
TAGCCTTTTTTTTTTTTTTTTAAATGTATGCTGTCATGATTAGAACTGTGTATAAGGAAATTCCTCAGACAGCATTACTGGTCGAGACTACTACACCAAA
ATCTTTGTTTGGCCCAGAGAATTGTACTTTGAAAGATGTGGACTCATGGACTTAGCATGAGTAGCAGTAACTTAACTTTATTCATTGCAGTTTCATATTC
AGAAGGGATGGGAAAACCCACTAGTTTTATTTAAATTTGCACTACATGTTTTTTTATGCAACATGCTAAATGAGCAGGTTTTAATTTATTGTGCAGTGCA
TCATGCTATGTGTTTGTTCACTCTATGACTGCTGTCTCGATTTCTTTGTTGTATTGCTGGATAGAGTAGGTACCAAAAGTATTGAAACTAGCAATAGCTG
CACTATAGAGCAGCTGCCTTCTGTCTAGCCTGTTCTATGGCTGGCAATAAAATCTTTCATGTTCATGTCTGTGTACCCTGTTTCTTGTGGAGGATGGGTT
CAAAATAAAAACGAATAAAGTTTTTTAAGAATTCAAAAAAAAAAAAAAAA
5' EST: CX906598 [+]ttggtggcgcgattgacgatatattatcccctcaacaggttggactctaagccgcatgtgaagatatattgcactggaatggacaaggatgtacaaagtc
atttttcttaacaagaaaccatcgtggcacaagtattttgtttgtgttctccaaccaaaaaagttagttattcagagtaactaaaaaaatacatgggtct
atcttactgcatcttcgtatagctggattaaatacacatatattttctgggattttacatagaagacaggacggccgctcaaagcgaggagttgccacca
atgccacccaaaatggcatcggtacggttcaccgttacacccaccaaaatggacgaaatgggggcaatctctgacaccagcccagatctcagttcatcct
cccgtgtgcggtttagctccagagagagtgtcagagatgccagccgcagtgaggcttacagtgacacctcactggcaaccacaggagtggaccaagctag
tgaaaggacctccaaccctatggacagtggagaagatcggagccaaacatcagtaacagaggagaacagtaagctgctagacattgaccgccggaagagt
tcagtgctttatcaaaatcacaccgatgatgaaggggactcttatgacagaaatctggccctgtttgaggaagaaatggatactcgaccaaaagtttcat
ccctccttaaccgcttggctaattacacaaatctgcctcagggagcaaaagaacatgaagaggaaag
3' EST: CX906597 [+]ttttttctttttttttgaattcttaaaaaactttattcgtttttattttgaacccatcctccacaagaaacagggtacacagacatgaacatgaaagatt
ttattgccagccatagaacaggctagacagaaggcagctgctctatagtgcagctattgctagtttcaatacttttggtacctactctatccagcaatac
aacaaagaaatcgagacagcagtcatagagtgaacaaacacatagcatgatgcactgcacaataaattaaaacctgctcatttagcatgttgcataaaaa
aacatgtagtgcaaatttaaataaaactagtgggttttcccatcccttctgaatatgaaactgcaatgaataaagttaagttactgctactcatgctaag
tccatgagtccacatctttcaaagtacaattctctgggccaaacaaagattttggtgtagtagtctcgaccagtaatgctgtctgaggaatttccttata
cacagttctaatcatgacagcatacatttaaaaaaaaaaaaaaaaggctaagcttttacaggaatgtcattagaacacccaaggtttttgtggccgatgt
ggaagatcatttttggtgttcactgggttataaagagatctgataaagctgagttgttccagtcacaatatgcacaaacgcgtgaattcccttaagcta
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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