XB-CLONE-3257783
Clone Name: IMAGE:7686196
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Gene:
pzp
,
a2ml1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122896 [+]
CAGCACGGAGCTACAGACACAATGCTGCTAAGGGCCCTCCATGTGGGGCTCCTCCTGCTGCAGCTCTCTGGGGCACTGGGGGCAAAGCTGCAATATGCAG
TCATCTTCCCCTCAGAGATGGTCTACCCCCAATCTGAACAAGCCTGTGTGCACCTGGAGGGGGCAGATGGAGGCAGCAGGATACACATCACCCTACAGAT
GGGCAAACATAACACAACGGTGTTGGAGAAGACATCACAGCAGAAATCCTACTTCAGATGCATTACATTTGAGGTGCCGCCTCCCACTAATGGGAATGAA
GAAGTGGCCACCATGCAGGTTTCCATTGAGAGTGGAGGGGAGACAATAAGCAACAGCCGTAGAGTTCTGGTGAAGAGAGCAAGAACCAGTCTTTTTGTTC
AGACGGACAAAGCTGTATACAAACCAGGCCAGACAGTGAGATTTCGAGTGGTTTCCCTTAAAGAAAACCTGCAACCAGAAGAAAGTCAGGTGCCAACCAT
TGAATTACAGGACCCAGGGAAGAACCGTATTGGCCAGTGGCTGAACGTGAGTCTCCAGCAAGGAATCGCTGAGCTCTCACTGCCCCTCTCTTCCGAGCCC
TCATTAGGAGAATATTCCATTCGTGTAAAGGACACTGTCCATCACTTCAGTGTGGAAGAATACGTTCTGCCCAGATTTGAGGTCACTCTCCAACTCCCCA
GAGTGGTTGCTGTTAACAGTAAACAATTCGCTGTTAACATCTGTGCCAGGTATACTTATGGGAAACCAGTGCAGGGAACCTATAAGGTCAATGTGTGCAG
GAAGCATTACAGCTACAGAACCATGGTTGCCAGTGACCAAGGGAGAGATCTTTGTGCCGATTTCAGTGGAACGCTGAGTCAGTCCGGTTGCTCCACCTTG
GATGTGCAGTCTTCTACCTACAATCTTCTATTAAGCGAATGGCAGTTGAACCTGGAGGGGACTGGCTCAGTTCTAGAAGATGGGACAGGAATTGAGATTT
CTGCATCTGGTAGATCCCAGATTTCAAGCCAACTCACCACTGTGACCTTCCTGGATGCTGACGATACTTACAAGCCTGGAATCTCATATATTGGCACCGT
GAAGGTTGTAGATGCCAGTGACACCCCCATCCCAAACCAAACAGTGTATCTGACCAGTTTAGATGGAATGGTGAACCAGACCTTGGAGACAGATGAGAAT
GGACTTGCTCCCTTCAAACTAGAGAACACAGCAGAGTGGAAGGTTAAGGTGTTCCTGACGGCCAAGATACAAAAAGAAGAGCCTTTAAGAAAGTATCAAT
ATGGTGTCATAAACCCATCACATGGGTCAGCGTTTCTTACAATCAGCCGCTTTCATTCCCTCAGCAACAGTTTCCTAAAACTTTACGCCCAGAAGGGGGA
GCTTCTCTGTGAGGGGCAACAAGAAGTCCGGGTGGAATATCTTATCAAGCGCACTGTGCTGGAAGAGGGGGCAAAGCACTTGGAGTTACACTACTTGACA
GTTTCTAAAGGGGCCATAAAGGATTGTGACTTTTTGGAAGTTCCAATAAATAATGAAGATGAAGTCCTGAGGGGTGAGGTCTCCATCAAGCTCGATCTCA
GCGCCGACGTCTCTCCGACTCTTCGAGTTCTTACCTACATTTTACTCCCTGGTGGAGATATGCCGGCAGATTCAGCAACGTTCAAGCTACAAAGGTGTTT
TAGGAACAAGGTATCAGTTGGCTTCTCCCCAGATGAAGTTCTCCCAGGAGCTGATGTTTCCCTACAAGTCCAGGCAGCCGCCGGGTCCCTGTGTGGCCTG
AGAGTGGTGGATCAGAGTGTGGTGTTAATGAAACCTGACAAAGAACTGACGGCCAATAAGATTCATGATCTCTTCCCCTCCCGTGATTTTGGGTATTATG
ATTATCGGATTCAGGAATATGATGATCACTGTCCGTTCTTTCCTTATTGGCGACCTTGGAACCGATTTTTCAAACGTTCTATTCAACCGTGGCATTTTCC
AGAGGAACGTGATGTCTACAGTCTCTTCAAGAAAATGCGGATAAAAATAATCACCAACGCTGACATTAAGAAACCTGTGGAGTGTCAACATTATGATTTT
AGGGGGCCAGGGCTTCCGGGGATGCCAGGGATACCAGGAGACCGCAGAGTTGTAATGGCTTCTCCTAGTATTGAGAGGGACGTCATGGTAGGGGCAGCTA
TGCCAGACACGATCCAACAACCAGAGCCTGAGGCAGCTGAAATTATCCGAACGTACTTCCCAGAGACGTGGATATGGGAGCTGGCGCCTGTGGGTGAGTC
GGGTAGAGCCGAGGTCCATCACTCAGCCCCAGACACCATCACCGAGTGGAATGCAGGAGCTTTCTGTATGGGACCCAGTGGGTTTGGCATCTCCCCACCA
ACCTCCCTTCGAGTCTTCCAGCCCTTCTTTGTGGAGCTCACTCTGCCGTACTCTGTGGTGCGAGGGGAGTCATTCACTCTCAAGGCCTCTGTGTTTAACT
ACCTCAAGCAGTGTATAAAGGTTCAGACCACCCTGCTGCCTTCACAAGAGTTAGAGCAGGAACCATGTGCTGACTGTCAGTATAGCAGCTGTCTCTGCGC
AGAGGAAAGCAAGACCTTCTACTGGAACCTTAAAGCCTCCAACCTGGGGGAGGTGAATGTGACGGTAAAGACCGAGGCTCTGGATACTCAGGACTTGTGC
AATAATGAATTCCCTATTGTCCCCAAGCAGGGGAGGTCAGACACTGTGGTTAAGCCACTACTGGTTCAGCCGGGGGGAGTCCTGATAGAGAAATCTCACA
GCTCTCTGCTCTGCTGCCAAGCTGGAGAAGATCATCCTAAAACAGAGGAGATATCTCTTAAGGTTCCTGAAAATATTCTGAAGGATTCAGAAAGAGCATA
TGCGACAGTCCTAGGAGACCTGATGGGAACAGCCATGCAGAACCTGGGCCGGCTTCTGGCCATGCCATATGGATGTGGGGAGCAGAACATGGCCCTCTTT
GCCCCCAATATCTTCATTCTTCAGTATCTGACAAAGACTCGCCAACTGACCCCGGAGGTCCAGAGCAAAGCCAAAGGATTCCTTGAAAGCGGGTACCAGA
GGCAGCTGACATACAAACGCAATGACGGCTCATACAGCGCATTTGGACAGAGGGACAAAGAAGGCAACACATGGCTGACTGCATTTGTAGTGAACTCCTT
CAGCAAGGCTCGTCCCTACATCTTCATAGATGAAAGCCACCTCAACCATTCATTCTCTTGGCTGAGGGACAATCGTAATGAGAGCGGCTGCTTCCGGAGT
GTGGGGAGACTCTTCAATAACGCTTTGAAGGGAGGAATAGATGATGAAACCTCTCTCTCTACTTATGTAACAATGGCTTTGCTGGAAGCTGGGGTATCTG
TACAGGACCCAGTGGTGGCAGATGCTTTATCCTGCCTGAGGAAGGCTGCCAAAGATGTGAGCAGTGTGTACACCCAGGCTCTGCTGGCCTACACTTTCAC
TCTGAGTAAAGATATTGAACTCAGGGAGATGCTACTGGCTAAACTGGAGGAGAAGGCTGTGAGGAATGAAGGACAGTTACACTGGGAGAGGAAACCTGCC
ACAGTGCCCTCTGACCTCCCATACTGGTACCGGGCTCCCTCAGCCGAGGTGGAGTTAACCTCCTATGTGCTGCTGGCCCATCTCTCTGGCCCCAACAAGG
ACCTGGGCAAAGCTTCTGAGATAGTCAACTGGTTGAGCAAGCAGCAAAATCCTTATGGGGGGTTCTCTTCTACTCAGGACACGGTTGTAGCTCTACAGGC
TCTTGCTAAGTATGGTGAGGCCACATTCACTGACAAGGGAGATGTCACAGCCACAGTTACTTCCAAGACTGGGTTCCATCAACAGTTCCATGTGGATCAG
ACTAACCGGCTCCTGCTACAGAAAGCCTCACTGCCAGATATCCCTGGGGAGTACTCTCTGTCAGCCACTGGGAGCGGCTGTGTGTATGTACAGACTGTCC
TGAGATACAACATTCCCCCACCTAGAAGTGACGCCACCTTCTCAGTACGAGTGGAGACAGAACCAGACAAATGTCCTCAAGATCAACTAAAAGTGCTGAA
GATAAGCATGACTGCCCAATATACAGGATCTCGGGAGAAATCCAACATGGCGGTGATCGAGTGTAAGATGCTCTCTGGATTTATCCCAGTGAAGAGCTCA
GTCAGAAAACTGGAGAAGAGCAAAACCATAAAGCGGAGTGAGATTCTGACAGAAAAGGTGACATTATACTTAGATGAGCTGGGACATGAGCCGCTCCATC
TCTCCTTCATGGTGGAACAAGACATTGTGGTGAAGAACCTAAAGCCGGCCACGGTGAAGGTCTATGATTATTATGACACAGAAGAAGAGGCAGTTGCCGA
GTACAACTCCCCCTGCGGCTCCGATGGAGACAAAGGCAACGCAAAATGAGATCTCTACAGGCAGATGCATGAGTGGAACAAAAACGTTACAATATATTTG
CAAATCATGAAATTAAATATTTTTGTCGCTTTCAAAAAAAAAAAAAAA
5' EST: CX906605 [+]acggagctacagacacaatgctgctaagggccctccatgtggggctcctcctgctgcagctctctggggcactgggggcaaagctgcaatatgcagtcat
cttcccctcagagatggtctacccccaatctgaacaagcctgtgtgcacctggagggggcagatggaggcagcaggatacacatcaccctacagatgggc
aaacataacacaacggtgttggagaagacatcacagcagaaatcctacttcagatgcattacatttgaggtgccgcctcccactaatgggaatgaagaag
tggccaccatgcaggtttccattgagagtggaggggagacaataagcaacagccgtagagttctggtgaagagagcaagaaccagtctttttgttcagac
ggacaaagctgtatacaaaccaggccagacagtgagatttcgagtggtttcccttaaagaaaacctgcaaccagaagaaagtcaggtgccaaccattgaa
ttacaggacccagggaagaaccgtattggccagtggctgaacgtgagtctccagcaaggaatcgctgagctctcactgcccctctcttccgagccctcat
taggagaatattccattcgtgtaaaggacactgtccatcacttcagtgtggaagaatacgttctgcccagatttgaggtcactctccaactccccagagt
ggttgctgttaacagtaaacaattcgctgttaacatctgtgccaggtatacttatggggaaccagtgcagggaacctataaggtcaatgtgtgcaggaag
cattacagctacagaaccat
3' EST: CX906604 [+]tttttttttttttttgaaagcgacaaaaatatttaatttcatgatttgcaaatatattgtaacgtttttgttccactcatgcatctgcctgtagagatct
cattttgcgttgcctttgtctccatcggagccgcagggggagttgtactcggcaactgcctcttcttctgtgtcataataatcatagaccttcaccgtgg
ccggctttaggttcttcaccacaatgtcttgttccaccatgaaggagagatggagcggctcatgtcccagctcatctaagtataatgtcaccttttctgt
cagaatctcactccgctttatggttttgctcttctccagttttctgactgagctcttcactgggataaatccagagagcatcttacactcgatcaccgcc
atgttggatttctcccgagatcctgtatattgggcagtcatgcttatcttcagcacttttagttgatcttgaggacatttgtctggttctgtctccactc
gtactgagaaggtggcgtcacttctaggtgggggaatgttgtatctcaggacagtctgtacatacacacagccgctcccagtggctgacagagagtactc
cccagggatatctggcagtgaggctttctgtagcaggagccggttagtctgatccacatggaactgttgatggaacccagtcttggaagtaactgtggct
gtgacatctcccttgtcagtgaatgtggcctcaccatacttagcaagagcctgtagagc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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