XB-CLONE-3257364
Clone Name: IMAGE:7794014
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Gene:
mthfd1l
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC159178 [+]
atgaaatcgcatccatgagccatgtggctgacctgccctaccggcgagtcactgactggtagcacacgcaatagataggcgtggggaagcgctgcgtttc
ctctttaccagttgtatcgctacataaacatcgctgcttgttcctccggtaccgatacaattgaacaagatgcgtcttctcctaatccgcacgatctctc
accccctgcctgcccatagggccgtggtacagcagggcaaaggatgcgccaggcgagcggctagtggggcaagtagcagccgggacgggcggcactccgc
tacctctgcttggaaatcagcccagcagcttcacgcatcggcagccagaaaaattattcagcgttcaaaggatgttttcggtggtctgcaaaaggaaaat
tctacttttaaaccagttcttgcaataatacaggcaagtgtggatgatctaaccttggagaccaccaagaattatgcagaggagctgggagttgatgtcc
ttcacataagtttgcctcaagagagccgtgatgaagaaattattgatgaaatcctaaagctcaatgaagacccaaaggtccacggtctcgtccttcattt
gactgatccaaacagcaaagttctaaatgctgttcgacctgagaaagatgtggatggtgtcacggatgtgaacttggggaagatggttcgtggtgacatg
caagaaggatttatatctcctatagccaatggtgtcatagatttgcttgagaaaacagctcaaacatctctgcaagggaagcatgtgttaattgttggag
ctgatgggtccctagaagcctctctacagtgcctgcttcagaggaaaggtgccacatccatagggtgcccatggcaaacacaaaacctgcagaaaaagct
taatcgggcagaaattgttgttcttggtagcacaaagccaaaggagattcctgtcagttggataaagccaggcacaattgttattaactgttctcacatt
gtactgtcaggagtttctgaacagtatgttgatggacttgctggtcatggtgttggaccacttgctgcagctttaagaatggagaacctagcagaaagta
gtagaagatggatggaggcacagagatataagaagtggaaccttcactgtttgaaacttcagccactttcaccagtcccgagtgatatcgagatttcccg
ggcacagagtccaaaagctgtcgatgtgcttgccaaggagataggattgcttgcagatgaaatagaagtctatggccgtactaaagccaaagtacgtctc
tctctgctggagaggttaaaggagcagccagatggaaaatacgttttagtagctgggataacaccaacaccccttggagaagggaaaagcacggttacca
tagggctggtgcaagctctgactgcacatactaatgtcaattcctttgcctgtctgagacaaccttcacaaggaccaacttttggtgtcaaaggtggggc
tgcaggaggtggatatgcacaggtcattccaatggaagagtttaacctccatctcactggtgacattcatgctatcactgctgccaacaacttgctcgct
gcggcaattgatgcccgaatcctgcatgagaacactcagtctgacaaggctttatataatcgtctggttccctccattaatggtgtaagagaattctccc
cgattcagattgcccggttacagagacttggtataaagaaaaccaaccctttgtccttgacagctgaagaaattagtacatttgttcgtcttgatattga
cccatccacaataacctggcaaagagtgattgacacaaatgaccgatttctcagaaaaatcacagtcggtcaagcaaatacagaaaaagggttcgtcaga
caggcgcagtttgatattgctgtagccagtgaaattatggctatcctagctctaacagacagcctgtctgacatgaaggaaaggctgggtaggatggtgg
tagccaatgataaaaaagggcaacccgtgactgcagaagatctgggggtcactggtgcgttgacagtattaatgaaagatgccataaagccaaccctgat
gcagacattagagggtactccggtgtttgttcatgctggaccttttgctaacattgcacatggcaactcatcagttttagcagacaaaattgcactgaag
cttgttggagaaaatggctttgtagtaactgaagctggatttggtgctgacattgggatggagaagttcttcaatattaagtgtagagcatctgggctgg
ttccgaatgttgttgttctagtggcaacaattagagctctgaaaatgcatggaggaggaccaaatgttactgctggtgcacctcttgcaaaagaatacac
cgaagagaatcttcagcttgttcagaatggctgctgcaatttgcagaagcaaattcaaattgctcagaaatttggagtccctgttgttgttgccttaaat
attttcaagacagatacttgggctgagattgaactggtgtgcaaaattgcaaaggattctggagcctttgacgcagtcccatgtaatcattggtcagtcg
gaggcagaggagctgtagaaatagcccaggctgtcaaggcagcagcaaaccaagagaataacttccaatatttgtatgacctcaagcttccaattgtaga
aaagatcaggaccattgctcagcaagtgtatggagcagctgacattgagctgtccccaaaggcagaatccaaaatagaagtctacactaaacagggtttt
ggaaaccttccaatctgtatggcaaagactcatctgtcattgtcccatcagccggagaagaaaggtgttcctacagggtttatactgccaatcagtgatg
tgcgagctagtattggtgctggtttcatatatcctctggtgggaacgatgagcaccatgccagggctaccaaccagaccctgcttctatgacattgacct
tgacgctgagacagaagaagtgaagggcttgttttaagaaagttaagatcccttcactgcctgcgcctgacttctccgcgtgatgcacaggaaagcttac
tacttattccagacaaagccaactggactgaggagcaccacacatacacacatttatgcacacacatttacttcccagagactctcaaggaactgggatg
tgaaaccacaggataagctgcagggatggattggctgttatttacctgaagtgtcttgttagaaagagctgcacttttcctttaatagacacgcataaag
tactccaatacgtgtagctgtttcttgctgggatgagattacctacagctaaaagatttatgcagtgcaaagtaggaaaacaatgaaaacaagcactaat
actcaggttttggcctattgcacacactaatacaagaatataaaattgccggggatccctctcaattctccaggttgatattgggacaatttgttaaaag
ttttgtataattgccaggtaaatgttcttgcctgggaaaggggggtgggtgcttctgccatgaggcaagctgagagagacaaaaagccactactggtatt
cacaggggatttcagtgcacacaaaatgtatttcagaccaatacatatttaaattttttggtgccgaaatacattgcgtgtgcactaaaaggctgattcc
atgcttgtcagaaagggaaagaaaggcagcagatcagcttttcttggtacaataagcctaaaagtacaaatactgtttttaacatggaatttcagccgtt
ctagtgcaaagagcacaagtgtggttcatgcactagtgatgcagccacttattacccctgaaaagagtgtgtggggtgggctaggcccccttagggcaga
atccccactgtatcacagatcagtcgacttaattcatccaaatgtgttctatgttcttgagcagttgaacactttctattccatgggatgctcagctgta
caagcactgctcggaataatacccccccactgcagaaaagatcatgttaaagctaaacatctacctattctttaacagcaataagtcaatcagccctttt
atgttctgcttgtcactacaagtagcagtgactgaaatgcctggccagtcaccataatatcatttgagcacaatgggaagcagcactggtagctgtaaac
tgttaagatttaatcagcagttctgcttaaccattttggggatacttatgcttcttcttgtcaacaataggaagctgtcttttggcattttaatcaccat
tgcttgtgacagtaccatgtgtgccatagtccttagtgttaatgcacctttattaaatgattaaagccaagatgcactatttcagactgttactctcttg
ctttttctgcttagatgattgctcacaagaactttcttaggctttactaaacatgacaaacttcttgccatgaatgttagaaatgaatgtgttctttaac
tgttttttcattaaaatgtcagctaaaaaggtttggctattataaaagacattatagttgtctttctggcagggtagatggtctgaggcaacaaaacgaa
gctaccttggacagagactgagccatattctataatttgctaatgtcgaagggatggaattcttatcaggaattcttatcaggagcagattttatgttct
cttccatctttcacttcaagcgttccttggtgtgagcccatgggtatgtgcgtgatgtgtgtgactttatatagctatttattaatgaactgacccttac
accatctcctcttcataccccaattcctgattctaggccaccatatttgttctcttactgacacctcccaacataacaaaatacatcttggttgggctgt
tagtttgtgcaggccgcacagtattgtctacaattcagttaaaaggtgtctgtgttatctttaccatatccctttaaggccagtctgtgtgtgagtgtca
gtaggtacacatgcggagtgagtggtgcaagtaagtagtgggctccctatggtttcaggcagctacaatgtctctggcactaggctggacagaggatatt
tcactgtgtttttctgaatgaataggaagtgtaacggtattttcaatttttttttttttgcagtaaagatatttcgccttttttgtctgctgtaacagaa
aatgtgctaagctttctctctttgcaaaaataaacagatctcattttcaaaaaaaaaaaaaaa
5' EST: CX907358 [+]atgaaatcgcatccatgagccatgtggctgacctgccctaccggcgagtcactgactggtagcacacgcaatagataggcgtggggaagcgctgcgtttc
ctctttaccagttgtatcgctacataaacatcgctgcttgttcctccggtaccgatacaattgaacaagatgcgtcttctcctaatccgcacgatctctc
accccctgcctgcccatagggccgtggtacagcagggcaaaggatgcgccaggcgagcggctagtggggcaagtagcagccgggacgggcggcactccgc
tacctctgcttggaaatcagcccagcagcttcacgcatcggcagccagaaaaattattcagcgttcaaaggatgttttcggtggtctgcaaaaggaaaat
tctacttttaaaccagttcttgcaataatacaggcaagtgtggatgatctaaccttggagaccaccaagaattatgcagaggagctgggagttgatgtcc
ttcacataagtttgcctcaagagagccgtgatgaagaaattattgatgaaatcctaaagctcaatgaagacccaaaggtccacggtctcgtccttcattt
gactgatccaaacagcaaagttctaaatgctgttcgacctgagaaagatgtggatggtgtcacggatgtgaacttggggaagatggttcgtggtgacatg
caagaaggatttatatctcctatagccaatggtgtcatagatttgcttgagaaaacagctcaaacatctctgcaagggaagcatgtgttaattgttggag
ctgatgggtccct
3' EST: CX907357 [+]tttttttttttttttgaaaatgagatctgtttatttttgcaaagagagaaagcttagcacattttctgttacagcagacaaaaaaggcgaaatatcttta
ctgcaaaaaaaaaaaaattgaaaataccgttacacttcctattcattcagaaaaacacagtgaaatatcctctgtccagcctagtgccagagacattgta
gctgcctgaaaccatagggagcccactacttacttgcaccactcactccgcatgtgtacctactgacactcacacacagactggccttaaagggatatgg
taaagataacacagacaccttttaactgaattgtagacaatactgtgcggcctgcacaaactaacagcccaaccaagatgtattttgttatgttgggagg
tgtcagtaagagaacaaatatggtggcctagaatcaggaattggggtatgaagaggagatggtgtaagggtcagttcattaataaatagctatataaagt
cacacacatcacgcacatacccatgggctcacaccaaggaacgcttgaagtgaaagatggaagagaacataaaatctgctcctgataagaattcctgata
agaattccatcccttcgacattagcaaattatagaatatggctcagtctctgtccaaggtagcttcgttttgttgcctcagaccatctaccctgccagaa
agacaactataatgtcttttataatagc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: