XB-CLONE-3257285
Clone Name: IMAGE:7686653
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Gene:
braf
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121876 [+]
AACCGAGGGGCCCTCACTGCTCAACGGGGACCTGCTGGAGTCCGAGACCGGGGGACTGGGCGGAGCCGGCGAGATGTGTGCGTCCGGGGGGTACCCGGAG
GAGGTCTGGAATATAAAGCAGATGATAAAGTTAACACAAGAGCACATCGAAGCCCTGCTGGACAAATTTGGAGGCGAGCACAACCCCCCATCTATATACG
TAGAGGCTTATGAGGAATACACCAGCAAACTGGATCTATTACAGCAGAGGGAACAGCAGTTACTGGAGTCTATGGGCAATGGAACAGACTCTACTTCTTT
GCCTTCAGAGCTGACACTTTCCTCGGTCCCATCTAATCTATCCTTGGCTCCTTCAGCTCTCTCAGCCCTGCGGACCCCCAGCGACCCCTCCCGTATCAAT
CCCAAGTCTCCCCAGAAACCTATTGTCCGAGTCTTCCTTCCCAACAAGCAGCGCACTGTGGTACCAGCTAGGAGTGGTGTGACAGTGCGAGACAGCCTAA
AGAAAGCCCTGATGATGCGAGGGTTAATCCCAGAATGCTGTGCTGTGTACAGGGTGCAAGATGGGGAGAAGAAGCCGATTGGCTGGGATACAGATATAAG
CTGGATCACTGGAGAAGAGTTACACGTGGAAGTCTTGGAGAATGTGCCGCTCACAACCCATAACTTTGTACGGAAAACCTTCTTCACGCTGGCTTTCTGC
GACTTCTGTCGGAAGCTGCTGTTCCAGGGCTTCCGCTGCCAGACGTGCGGGTACAAGTTCCACCAGCGGTGCAGCACGGAGGTGCCTCTTATGTGTGTTA
ACTACGACCGTCTAGATTTGCTGTTTGTCTCCAAGTTCTTTGAACACCACCCAATATCTCAGGAGGAAGCTCCGTTTGAAGAGGTCGCCCCAGCCGCTGA
GTCCTCGACTCCTCCGCCCCCTCCAGATGCCACAGGCCCCCTCTGTCTACCGAGTACTTCTCCTTCCAAATCCATCCCGATTCCCCAGCCGTTCCGACCG
GATGAGGACCACAGGAACCAGTTTGGCCAGCGAGATCGGTCGTCCTCGGCGCCCAACGTGCACATAAATACCATCGAGCCGGTCAATATTGACGACTTGA
TTCGAGACCAGGGCCTCCAGTGCAGCGAGGGAGCCCCCTCAGTCCAGCCGACGCGCTGCCATCGGAAATACCACTCGCGGGACCCCAGCCCACTCCTCCA
TACTCTCCCCAGTGAAATAGTCTTTGATTTTGAATCTGATCCTGTTTGCAGAGGTTCAACCCCCGGCTTATCGGCGACCCCCCCTGCCTCCCTCCCCGGC
TCCCTCACCAACGTCAAGGCGCTACAGAAATCCCCGGGGCAGCAGAGAGAGCGCAAGTCCTCCTCGTCCTCGACGGAAGACAGGAGTCGGATGAAAACGC
TCGGACGACGCGATTCCAGCGACGACTGGGAGATTCCCGACGGGCAAATCACGGTTGGGCAGAGAATAGGTTCTGGATCGTTTGGGACAGTGTATAAGGG
AAAGTGGCACGGGGACGTAGCAGTGAAAATGTTGAATGTAACGGCGCCAACGCCTCAGCAGCTACAGGCCTTTAAAAATGAAGTAGGAGTCCTCAGAAAA
ACGCGACACGTGAACATCCTGCTTTTCATGGGCTACTCCACGAAGCCCCAGCTGCGACTAGTGACCCAGTGGTGCGAGGGCTCCAGTCTCTATCACCACC
TGCACATCATCGAGACCAAATTCGAAATGATCAAGCTGATCGACATTGCACGACAGACGGCGCAGGGCATGGACTATTTGCACGCGAAGTCAATCATCCA
CAGAGACCTAAAGAGCAATAATATATTCCTTCATGAAGACCTCACAGTAAAGATTGGTGACTTTGGCCTGGCAACAGTGAAGTCCCGGTGGAGTGGGTCT
CATCAGTTTGAGCAGTTGTCTGGCTCCATTCTGTGGATGGCTCCTGAGGTCATTCGGATGCAAGATAACAACCCCTATAGCTTTCAGTCTGATGTCTACG
CGTTTGGCATTGTTCTATATGAACTGATGACGGGGCAGTTGCCCTACTCCAACATCAACAACAGAGACCAGATCATATTTATGGTTGGGAGAGGCTACTT
ATCTCCCGACTTCAGCAAAGTTAGGAGTAATTGCCCCAAAGCAATGAAGAGGTTAATGGCAGACTGTCTAAAGAAGAAGAGAGATGAGCGCCCCCTATTC
CCACAGATTCTCGCCTCCATCGAGCTGCTGGCACGGTCATTGCCAAAAATCCACCGCAGCGCGTCTGAGCCGTCCTTAAACCGCGCTGGATTCCAGACGG
AAGACTTCAGTCTGTACACGTGCGCTTCTCCCAAAACGCCTATCCAGGCGGGAGGTTATGAAATCTCACCAAGATGGGTAAATAACTTTAATGAACCTCT
TTTTTATCGGTGTCCAAATCAGCAGAGTATCAAAGAAATCACAAGGTAACGCCAGGGATATAGCGAGTGATCCAATGGCTTTTCCTCTTCTCTCTGCCCC
TATATATTAGTATGCGTGACAGTCAGAGGTGGGACCGACTATGGGATTTTGTCTGTGAGAGATTATTCAACTCCGTTCCTACTTGTGAATGGAGCAACTA
CGTCAATAACATTGGCCAAGGATTTACCTTCTATTGCCCAACTGGCCAGGTTCTCAGTGGAATGGGAAATGAACTTGATGCCTGGGAGTCAGACAGGAGG
TGGAAGTTTCTTTGCTGCAAAGGAGAATTCCTACTTAACCGAAACTGTTCATGGAGCGACTATGTTAATGCATTTAATGAAGATCTGAGATGGAAAGCAT
CAATTAATCATTATTTGGCTGGAGTTTTAAGTATCACTAACAGCCAAACCGAGGACAGACGATGGAGGTACTACTCATGTGAGAAATAAATCCCTGCAGA
CCAGCCATACAGCCATGTACCTCTCTCCCAACCAATGAGTAACACCGTAACATGAGCCTCGGTATCTAACAGTACCCATATACCTATTTGATCTCCCAAT
AAATCACGCTGTAGCATAAAAAAAAAAAAAAAA
5' EST: CX907482 [+]aaccgaggggccctcactgctcaacggggacctgctggagtccgagaccgggggactgggcggagccggcgagatgtgtgcgtccggggggtacccggag
gaggtctggaatataaagcagatgataaagttaacacaagagcacatcgaagccctgctggacaaatttggaggcgagcacaaccccccatctatatacg
tagaggcttatgaggaatacaccagcaaactggatctattacagcagagggaacagcagttactggagtctatgggcaatggaacagactctacttcttt
gccttcagagctgacactttcctcggtcccatctaatctatccttggctccttcagctctctcagccctgcggacccccagcgacccctcccgtatcaat
cccaagtctccccagaaacctattgtccgagtcttccttcccaacaagcagcgcactgtggtaccagctaggagtggtgtgacagtgcgagacagcctaa
agaaagccctgatgatgcgagggttaatcccagaatgctgtgctgtgtacagggtgcaagatggggagaagaagccgattggctgggatacagatataag
ctggatcactggagaagagttacacgtggaagtcttggagaatgtgccgctcacaacccataactttgtacggaaaaccttcttcacgctggctttctgc
gacttctgtcggaagctgctgttccagggcttccgctgccagacgtgcgggtaccagttccaccagcggtgcagcacggaggtgcctcttatgtgtgtaa
actacgaccgtctagatttgctgtttgtctccaag
3' EST: CX907481 [+]gctacagcgtgatttattgggagatcaaataggtatatgggtactgttagataccgaggctcatgttacggtgttactcattggttgggagagaggtaca
tggctgtatggctggtctgcagggatttatttctcacatgagtagtacctccatcgtctgtcctcggtttggctgttagtgatacttaaaactccagcca
aataatgattaattgatgctttccatctcagatcttcattaaatgcattaacatagtcgctccatgaacagtttcggttaagtaggaattctcctttgca
gcaaagaaacttccacctcctgtctgactcccaggcatcaagttcatttcccattccactgagaacctggccagttgggcaatagaaggtaaatccttgg
ccaatgttattgacgtagttgctccattcacaagtaggaacggagttgaataatctctcacagacaaaatcccatagtcggtcccacctctgactgtcac
gcatactaatatataggggcagagagaagaggaaaagccattggatcactcgctatatccctggcgttaccttgtgatttctttgatactctgctgattt
ggacaccgataaaaaagaggttcattaaagttatttacccatcttggtgagatttcataacctcccgcctggataggcgttttgggagaagcgcacgtgt
acagactgaagtcttccgtctggaatccagcgcggtttaaggacggctca
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: