XB-CLONE-3256946
Clone Name: IMAGE:7686448
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Gene:
fam185a
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161076 [+]
ggtgactgagagatgagttccctgttataccgcggcggctgcagctgcctgagctgggcagggagaagggcccgggtggttgctgcctgcagatggaagg
gaggccgggcagcggagcctctgaacaaacctctcaagcaatggactttgatagtcagcccccaaagccgcctttgtgtagagctgccctgcagtgtgag
ggtgagctcccaggaccccttcgcctatccacacgcagaccgggttttcgttacggttagtggggtggaccgtaatgtgcaccggggtctggatctggac
aacattgaagtgaagtacgaggagagctcccggcaggtggtcatccactcgcgggacattgacagccacacctgcgtggaggtcactacgccagtccgct
ttgatgtgaatatcaagacatcagggactggttctgttaatattaaaaaaattgaatgtgacaactgccacatagaaaccgagaaaggggacagtgttct
gcagtcagtcaagagccacgcagtaaacatacgcacagatggaggcaaagtgagctgcttggggacagtctatggaaatgtgaatatttatgctgcagca
caaagtgtaagcaagaatggaagatgccttttctgagagtgttaagattgaaaaattacagggaacatctataaacatatgcactgaagatggaacattg
aaagccaagtacctatatactgaatcgtcctccatatcttcggcttctggggacatccggctcggaagtgtgcatggtgatgtcaatctacacagccaac
atggaagtgttacagtaggctctctggatggcagtctgtctgcatctacttgtgatggcgctatggatatttatgtgagccaagtgggacaagttcatct
gaaatctgaggaagggtgcattacagtgagagtacccgagaagcttccaacgtcgctacagctggctggaaccacggttgatgtgagcccagacattcag
ctgaatgaaatccaaactacttccaaagagggccaggtgactctaacagcctccataaatgcagccaatggaagggagaggtggattaaagcagaaagca
aaagaggaacagtttccctgaagactcaaaactggctccaatctctaaagtttcacacaacctgaacatgtatgcactgacctgatggaaaattcagtgt
caatccggcttttggctttgccacttcaaatggctgtaaggttttctatagcagtggatcactttccctattacagatttgcatgttggcaggtttaatc
aggaaatgatctaccacacagttctgtttttgccatttaaggcttcttgtgcttaaagaggacattttctgtttctttgaaaagctgaggaatgattaag
ataaaagccatgctttattgtatgtataactgtatagtgtggtgggtagaacgccattacacatagcattgcatcatattatgttagcttatgcactgtg
ccttctgtactacatggtcccgatctacacagccatgtgaaggtttttttaatagaaatttagccatgttggcttctattcagggatctgctagaggaaa
aaaaaaacagtagttgtaggtttttaatccaacaggttttacagcttgaatatatacacgtacagactttgtctattttaattccaagtgataatacagg
taatagaatatcttgtatgtacacaatgctgtgttctaatgcaatattgtgcgttaaggacaatcttctgcgtgggtagcctgttatgtatgccaattct
tttaagaaaacacaattattcattgacgttataaactagaatatttttttctaaggatacaattcctgtttgttccaagttgggtacatagtcagtaaca
ctaatgtagggattgttcaaggtgactagacaaagtcctgtatgatggccactgtagttagcaatagacaaatcatattgtggtgtttacttacaatttt
atacacaaaactacaaaacttttctttaaattttatgattagcagaaactttacagttggccagaaattaagcatggcactggcaatgagagggttttgt
taaaaggggactttctaaataaattcaattaaaattctgttactttttagtatccccctctcagcaatctgtctctcttacttctttcgtcatgcaggag
ttggagtcagggtttctcattgcctagacagtgtattagcattagagcttactggctttcagctatctgtcatttcaaatctgattcactcctgggtgta
gagagacagatgctggtcggaggatactaaagagtacctagattatttcaaaatcagtacagcatgttaacggatgttaattagaaagtttcttattttt
atgagaagcttatattaaacttttatcaccatttttttcccaaaaaaaaaaaaaaa
5' EST: CX908081 [+]gtgactgagagatgagttccctgttataccgcggcggctgcagctgcctgagctgggcagggagaagggcccgggtggttgctgcctgcagatggaaggg
aggccgggcagcggagcctctgaacaaacctctcaagcaatggactttgatagtcagcccccaaagccgcctttgtgtagagctgccctgcagtgtgagg
gtgagctcccaggaccccttcgcctatccacacgcagaccgggttttcgttacggttagtggggtggaccgtaatgtgcaccggggtctggatctggaca
acattgaagtgaagtacgaggagagctcccggcaggtggtcatccactcgcgggacattgacagccacacctgcgtggaggtcactacgccagtccgctt
tgatgtgaatatcaagacatcagggactggttctgttaatattaaaaaaattgaatgtgacaactgccacatagaaaccgagaaaggggacagtgttctg
cagtcagtcaagagccacgcagtaaacatacgcacagatggaggcaaagtgagctgcttggggacagtctatggaaatgtgaatatttatgctgcagcac
aaagtgtaagcaagaatggaagatgccttttctgagagtgttaagattgaaaaattacagggaacatctataaacatatgcactgaagatggaacattga
aagccaagtacctatatactgaatcgtcctccatatcttcggcttctggggacatccggctcggaagtgtgcatggtgatgtcaatctacacagccaaca
tggaagtgttacagtaggctctctggatggcagtctgtctgcatctacttgtgatggcgctatggatatttatgtgagcca
3' EST: CX908080 [+]tttttttttttttttgggaaaaaaatggtgataaaagtttaatataagcttctcataaaaataagaaactttctaattaacatccgttaacatgctgtac
tgattttgaaataatctaggtactctttagtatcctccgaccagcatctgtctctctacacccaggagtgaatcagatttgaaatgacagatagctgaaa
gccagtaagctctaatgctaatacactgtctaggcaatgagaaaccctgactccaactcctgcatgacgaaagaagtaagagagacagattgctgagagg
gggatactaaaaagtaacagaattttaattgaatttatttagaaagtccccttttaacaaaaccctctcattgccagtgccatgcttaatttctggccaa
ctgtaaagtttctgctaatcataaaatttaaagaaaagttttgtagttttgtgtataaaattgtaagtaaacaccacaatatgatttgtctattgctaac
tacagtggccatcatacaggactttgtctagtcaccttgaacaatccctacattagtgttactgactatgtacccaacttggaacaaacaggaattgtat
ccttagaaaaaaatattctagtttataacgtcaatgaataattgtgttttcttaaaagaattggcatacataacaggctacccacgcagaagattgtcct
taacgcacaatattgcattagaacacagcattgtgtacatacaagatattctattacctgtattatcacttgggattaaaatagacaaagtctgtacgtg
tatatattcaagctgt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: