XB-CLONE-3256938
Clone Name: IMAGE:7686736
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Gene:
ednra
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121283 [+]
GTCTTGCACAGGAGGTGGAGATTATCCATCCCACCCAGCCAGAGGTCTGTGACAGGATAGATCGGATGGAGCTAAGAGTGCAGCCTTAGTATTGCAGTGG
ATTGAGGTGGGAGAGGAGAGTCTGCTGGATAGAACTTGGCCGCTTGCTGTCCCCATGTGTGTGCTTTGTCACATTCAGTTGCATTGAGACCAGCGCAGCC
TGGAAGTAAAAGGATTGTATTGGACAACGGCTTCATTACATGGGTGATCAACTGGAATACTGACATTAAAAAATACATCATTTCGATTAATCAAAAAAAG
AAAAAATCAGGATGGGAGGCGACATCTTGAGATTCACCGTGCTAGTGGTATTGGCTGGAATTGCAGTCAGCAGCAGTTTTGGGGAGCACTACCAAAATCG
GACTGATGCCAACACAGATTTCACAATGCTAAATCGGAGTTACACTCCTCCTGTAAGAAGAATGAATAGATCTGAGTTGTGCCCAGAAAAGACAAAAATC
AACCATGTCTTTAAATATATAAACACCATCCTATCGTGCGCTATTTTCATCGTTGGAATGGTTGGCAATGCAACCCTGCTCAGGATCATTTACCAGAACA
AGTGTATGAGGAATGGCCCAAATGCCCTGATAGCAAGCCTGGCTCTAGGAGACCTCATTTACATTGTTATTGACATCCCTATAATTGTCTTCAAGCTGCT
TGCCCAGCGTTGGCCTTTTGATCAGTCTCCTGTTGGATCGTTCCTCTGCAAGCTGGTTCCCTTCATTCAGAAAGCTTCAGTTGGAATCACAGTGCTCAAT
CTGTGTGCCCTGAGTGTGGACAGGTACCGAGCAGTTGCATCCTGGAGCCGAGTTCAAGGCAGCGGAATTCCTCTTATCACAGCCATTGAAATCATCTCTA
TCTGGGTTTTGTCCTTTGTCCTGGCAGTTCCTGAAGCCATTGGCTTTGTTATGGTGCCCTTTGAGTATCGTGGTGAAGAATTCCGAACATGCATGTTCCA
CGCAACAAGCCCATTTATGATGTTCTACAAAGTTGCAAAAGACTGGTGGCTCTTTGGGTGCTACTTTTGTGTGCCTTTGGCTTGCACTGGGGTGTTTTAT
ACTATGATGACCTGCGAAATGCTGCACCAGAGGAAAGGAAGCCTACGGATTGCTCTTAGTGAGCACCTGAAACAGCGCCGTGAAGTTGCAAAGACCGTTT
TCTGCCTGGTAGTAATATTTGCCCTTTGCTGGTTCCCGTTACATCTCAGTCGAATTATTAAGAAAACAATTTACAACGAGTTGGATCCCAGCCGCTGTGA
GCTCCTCAGCTTTTTGTTGGTAATGGATTTCATCAGTATTAACCTAGCAGCGCTCAACTCCTGTATCAATCCTATAGCTCTCTATTTTGTGAGCAAGAAA
TTTAAAAACTGCTTCCAGTCATGCCTTTGCTGTTGTTGCCAGTCCAAGACACATATTAATACAGCTCCCATGAATGTAACAAGCATCCAGTGGAAAAACC
ATGAGCAAAATTACTACGGTGCAGATAGGAGCATTCACAAGGACAGCATCAATTAGCCCCACCCCTGAATAACGTGAAACCTCCCATGAAATTCACAGGT
GGCTGCTTCTTACAGTAAATCCACAAAGAGTGTCAGAGAAACAAAGATCCTGCTGGCGGTGTTTGCATCTTCATTCCATTACGGACGGTTCTTTCTGTAT
TTAAGCTCCACCCATCTGTTCTCTTATTGGCTGTAATACAGTCTTCACCTTCCACACCCAGCATATGCGCACAGCTGGACTGTCTCTATGAATCTTCCTG
CCCTTTGGAAAGAGACATTTTTACTGGCCTGCAAACATTTTCATTACACGATAAACATGAAGGAATTCATTTGCCAGTTCTCTCTCGAATTACTGAGACA
AGTGTCCCGATATATTGGAAAGCTTTACAGGCCATGCTAGCAACAGTGTTTAAAGGAGTTTAAGCAGACTGCAGTATTTCTATTGAGTTATTATATATAA
TATTATAACTGTATAGATCCATTTCTATTTAATACCATAACTCTTTAAAAAATATATTTTTATTTGAGCACAGTGGAATGTTGAAGCATTCGAGCATGTG
TCGTGCTGACATTTTGTCCCAAATGTAAAATGCTGGGTAGTAAGAAAAATGTTGGACTTTTTGCCAAAAAATTTAAAAAGCGTGTTTTATAGCACTTCGT
AATCTGGAGCCTTGCTTTCTGTAGATAGTGGGTTTTGAAAGTGCATGTCGGGAGAAACCACAATATTCTATTAACTAAACAAAGAAATTCCTTTGATGAT
ACCCAATGCACAAGTCCCAAAGTGTTCAGGTAAACACAAATTATATCCACTTACAGTTCATTTACGGAATTTTTTTTTTGGTTGTTATTAAAGCTGGTTT
GCATTTTCTGCCATATGAAACATTATTTTCTATCCTTTGTATATGGAAAAATGTATGTAGCTGTTTAATCAGGCTTTTGGAGTCACATAAAACAGTGCTC
AGCTTAACTTACTCCAAAGAGGAAATGAATGTTTTATTCATTTTTCAATCTCTATTTGTATAGAATATCTGCGCTCAGCAGTGAATGATGTCTGTTTACA
ATGTTAGTACATTTAACCATGCAAAACGCTCATTAGAGAATCAGGGTGAATGTTCCATACTCACACCACTTTAAAGAAGTCAAAACATCAATAGTGTAAT
AGAATCTTAAGTGCATTTTTTTTTCAATGTGAAATGAAGAAATACTTTATGGCTAGAACATTCCACCACGGACGGCACATTGATAAACTGAAAGCGGAGA
CAACATTTCAATAGGAAATGTTCCTCTAAGAAGACATCTGGCTTGCTTATTGTTATGTACAAAAGCAATTAAGAGACTATTTAATGATGTATAAAGAATA
TAAAAATATAATTTTGTAATTTACTCTCTCTGCATTTAAAACACATGTAAAATTCTGTATATACTATTTTTTTAAGTATTAATAACAGTCTTCTATGTAA
TCTTTTAGACAGTTCTCTACAATAATGGTTATGGGCATGTATATGTAGAAGCCCCAAATAGCTATTTATTATATTATTTCTGAGCCATTTCCCCAGGTAG
GACAATAGATATCAATCTGAAGCTCATGGAATCGTGGAATTCCTTGCATTACACTCAGGAGCATAGTTACAGGGAGTGGAGGGGTTGTGACTACAACTAG
GCCCCAAAGCCTGGGGGAGCAACATTGGTTATAAAATGGGCACAATAAAATTAAAAGTGCAGCTAAAAGGGTCAGTGCACAATCCCAAATTTAGGGACTG
GGGTTATGGATGAAAAACCAGGAGTGATTATGGAGAATATGTTTTTGGGGGTGACAAGAAAAAATCTGCATTGGAGAACCATGGTGCTGTAGGTATGCTT
CTGCTAACACAGTCAATAAAGGTCTTCTATAAGTAGAAAGAAAAAGTCATAGGATTTCAACATGGAAGTACAATGGCATTATCTTCCAGCATAACAACCC
CTCACACTTTATGTATGTAGAACTGTAACACTTTTTGACATGACTGTTGGCTTATTGAAGGTCTTCAAATTGTAATTTATTCAACAGGCACAAGTTATGT
CATGGGCAATTGTTAAGTGAATGTTAGATACAGTAAATGCATTAAAAGCTATAAAGTTCATATTCATATATAGAATCTCTTTTTTACATGAACAGTTTTG
ATATATGGCTGTGTATGTTGTCATGCACTTGTTCATTTAGAGCATATTTGGCTCTAATAAAGAAAAAATGAATATGCTCTAAAAAAAAAAAAAAAA
5' EST: CX908092 [+]tcttgcacaggaggtggagattatccatcccacccagccagaggtctgtgacaggatagatcggatggagctaagagtgcagccttagtattgcagtgga
ttgaggtgggagaggagagtctgctggatagaacttggccgcttgctgtccccatgtgtgtgctttgtcacattcagttgcattgagaccagcgcagcct
ggaagtaaaaggattgtattggacaacggcttcattacatgggtgatcaactggaatactgacattaaaaaatacatcatttcgattaatcaaaaaaaga
aaaaatcaggatgggaggcgacatcttgagattcaccgtgctagtggtattggctggaattgcagtcagcagcagttttggggagcactaccaaaatcgg
actgatgccaacacagatttcacaatgctaaatcggagttacactcctcctgtaagaagaatgaatagatctgagttgtgcccagaaaagacaaaaatca
accatgtctttaaatatataaacaccatcctatcgtgcgctattttcatcgttggaatggttggcaatgcaaccctgctcaggatcatttaccagaacaa
gtgtatgaggaatggcccaaatgccctgatagcaagcctggctctaggagacctcatttacattgttattgacatccctataattgtcttcaagctgctt
gcccagcgttggccttttgatcagtctcctgttggatcgttcctctgncagctgg
3' EST: CX908091 [+]ttttttttttttttttagagcatattcattttttctttattagagccaaatatgctctaaatgaacaagtgcatgacaacatacacagccatatatcaaa
actgttcatgtaaaaaagagattctatatatgaatatgaactttatagcttttaatgcatttactgtatctaacattcacttaacaattgcccatgacat
aacttgtgcctgttgaataaattacaatttgaagaccttcaataagccaacagtcatgtcaaaaagtgttacagttctacatacataaagtgtgaggggt
tgttatgctggaagataatgccattgtacttccatgttgaaatcctatgactttttctttctacttatagaagacctttattgactgtgttagcagaagc
atacctacagcaccatggttctccaatgcagattttttcttgtcacccccaaaaacatattctccataatcactcctggtttttcatccataaccccagt
ccctaaatttgggattgtgcactgacccttttagctgcacttttaattttattgtgcccattttataaccaatgttgctcccccaggctttggggcctag
ttgtagtcacaacccctccactccctgtaactatgctcctgagtgtaatgcaaggaattccacgattccatgagcttcagattgatatctattgtcctac
ctggggaaatggctcagaaataatataataaatagctatttggggcttctacatatacatgcccataaaccattattgtagagaactgtctaaaaaata
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: