XB-CLONE-3256909
Clone Name: IMAGE:7686550
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Gene:
dstyk
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC154679 [+]
CAGGCTGCAGTTGAAATGCAGAAAGACGGGACGCGTTCTTCCCGGAGAATGGAAGAAGGGGACCGCCGGAATGGCTCTACTGGGAGCTCCGGCTCCGTCT
CCAGAGAGCTGGGCCGCGGCTTTTCCTATTACAACAAGTACTTGGCTAGGCTGCAGCAGAACCTACGGGACACCAAGCGCTTCTTCCGTGACATCAAGCA
TACTTACAGCGGGGCGCCCGGTGGCCCGGACACCGATATCAAGGGAGCAGAAGGAGATTTAGGACAACTGCACAGCATCACCTTTCCCAGGCAAGAGGAG
GAATATCTAAAGCTCACTGTTCGCTGTCGCCCTTGTATCTTTATTCTGGGACAGAGCTGCAGTAGCCGGGGGCGTGTAGCCAATTCCTTGCTAGGTGACC
AGCTGCTCCCTATCCTCACCCATTGTGGTGCAGAGTGCTGTAAGAGAAGACGCATCCGTTTTCGTTATGGAAAACAGACTTTGGTGAGCCTGGCCCTGCC
AGAGCAGTATGAACTTGTGCATGAGCTGGTGGCACATCAGGGCAAATGGGATACCATTCCAGAAGAAGATCTTGATGTACCAGAAGATGAGGAAGACCCT
GCACATAGACTGGCTGAGCTGGAAGTGACTCTGCCTCACCAGCTACTTCAGGATGTCGATATTGTGGTTTCCCCCTGCCGTAGCTCCCAGGCAGTATCCA
TGACTCTAGAGGACTATGTTGACCACGTTCAGTCAATTGTTGTATATGCAGTATGCGAGGAAAGATTGTCCCAGCAGGATGAAGAAGAACTAACTGAGAT
AAAAGAGAAGTATAAACTGCCAGTATTTTTCATCCGGACATCATCAACAAGGGACAGGCTGATTGGGGGATCTGAAGGTGTACGTTCAGCTCTGTATGAA
CAGCTGATGGAGCTAGGATTTCTGAAAAGGGGCTTATGTAACTGTGGAGCTGCAGGATCAGGCAGCACAGCCCCCAGTATGCTAGTGGAGCAGTTTGAGA
AGCTACGGCAATTAAGTACCTTTTCAAGGCAAGTGTTACAGATGCACTTGGTAGATGCTGCAATAGTTCTTAACATGGTTCATAGCCGATGTTTGGACTT
GTTCATCAATAAAGCTTTTGACATGCATAGAGATCTTCAGATAACACCAAAGCGACTGGAGTACACTAGACAGAAAGAAAATGAGCTGTATGAATCTCTA
ATGCGAATCTCTGACCGTAAGCAAGAGGAATTGAAAGACATGATTGTGGAGACCCTAAACAGCATGAGAGAGCAGCTTCTTGAAGATGCCGCAAATATGC
AGTTCAAAGACATTGCAATACCGCAAAATGGTGAACCTGTTAGTGTACAAGAAGTTAAGTGCTGCATCTTCCAAATCAAGGAATTGATTATTTCAAGGCT
TAATCAGGCTGTGGTTAACAAGTTGATCAGTTCAGTGGACTATCTGCGTGAAAGCTTTGTCGGAACATTGGAGAGGTGTCTTCGCAGCCTGGAGAAGTCT
CAACAGGAGTCCTCTTCACATGTTACCAGCAACCACCTGAAGCAGATATTAAATGCAGCTTATCATGTGGAAGTCACTTTTAATTCAGGTTCTACTGTTA
CCAGAATGGTGTGGGAACAAATTGTGCAGATCATCCAGCGCATCACCTGGGTGAGTCCTCCTACCATCACCCCAGAATGGAAACGCAGGGTTGCACAGGA
TGCTATAGAAAGTCTAAGTGCTTCTAAACTTGCCAAGAGCATCTGCAGTCAATTTTGCAAGCGACTAAAAAGTTCCCATGAGGCATTTGCTGCATCTCTC
AAGCAGCTGGAGGTTGGGCACTCTGGTCGACTGGAAAAAACGAATGATCTGTGGTTAAGGGTTCGTAAAGATCATGCACCACGTCTGGCACGTTTGTCTT
TGGAGAGCAGGTCCCTACAAGATGTTTTGCTGCATGGAAAACCAAGAATAGGTCGTGAATTGGGGAGAGGACAGTATGGAGTGGTTTACCTGTGTGATAG
CTGGGGTGGGCACTTCCCATGTGCTCTCAAGTCTGTTGTCCCCCCTGATGAGAAGCACTGGAATGACTTGGCTTTGGAATTTCACTATATGAGATCCTTG
CCCAAGCATGAGCGTCTGGTTGATTTACATGGTTCAGTAATTGATTACAGTTATGGTGGAGGCTCCAGCATAGCAGTTCTGTTAATCACAGAGCGTCTGC
ACAGAGATCTCTATGTAGGCCTTAAGACTGGCCTTTCTCTAGAGACACGGTTACAGATTGCTCTAGATGTAGTAGAAGGAATCCGGTTCCTGCACAATCA
AGGACTTGTACACAGGGACATCAAACTGAAAAATGTTCTGCTGGACAAAAAACACAGAGCTAAAATTACAGATCTTGGATTTTGCAAACCAGAAGCAATG
ATGTCTGGCAGCATTGTGGGGACACCAATACATATGGCACCTGAGCTTTTCTCTGGAAAATATGACAACTCGGTGGATGTATATGCATTTGGAATTCTTT
TTTGGTATATCTGCTCTGGATCTGTAAAACTTCCAGAAGCCTTTGAGAAATGTGCAAGTAAAGACCATCTGTGGAACAACGTCAGAAAAGGTGCACGCCC
AGAAAGGCTGGCCATCTTTGATGAAGAGTGTTGGAAACTAATGGAGGCATGTTGGAATGGGGACCCCTCTCAACGTCCACTAATGGGCATTGTGCAGCCC
ATGTTGCAGGGCATCATGGACCGACTCTGCCGCGCACAAGAAACTGGCAAAGCTTTGGAAGATTCTACATGATAAAGAACTTCATCTTGCTCTTACTATG
TGTTCTTGGTTACTAGAAGCACCACCTACTGCTCCGCATTTACTGCCATGGAGACACAGATCTCTGTACTGCCTTTTTGATTTGGGATGCAGACTGAAAG
AAGTGAAGATGGAATTTCATGGTCCAGTACTTCCAAATATAAGCTATTAATAAATGCAGTGTTAAGATCTTCTCCATCTTTCTTTTCTCAAGGATGGCGC
GGAGGCTTGGAAAAGAATGTGGTTGTTTTCTAAGCTGGTTGCATCTTTCAGAAACATCTGCTCTAGTTAGTAAGCCAGCTCCTTGTTCTCACTGGAAGAT
ACAGAAAGTGGAGTGATTACTTTGGATAACCAATAGTGGCTCCAGGGTTAGGTTCTAAAAAAAAAAAAAAA
5' EST: CX908130 [+]caggctgcagttgaaatgcagaaagacgggacgcgttcttcccggagaatggaagaaggggaccgccggaatggctctactgggagctccggctccgtct
ccagagagctgggccgcggcttttcctattacaacaagtacttggctaggctgcagcagaacctacgggacaccaagcgcttcttccgtgacatcaagca
tacttacagcggggcgcccggtggcccggacaccgatatcaagggagcagaaggagatttaggacaactgcacagcatcacctttcccaggcaagaggag
gaatatctaaagctcactgttcgctgtcgcccttgtatctttattctgggacagagctgcagtagccgggggcgtgtagccaattccttgctaggtgacc
agctgctccctatcctcacccattgtggtgcagagtgctgtaagagaagacgcatccgttttcgttatggaaaacagactttggtgagcctggccctgcc
agagcagtatgaacttgtgcatgagctggtggcacatcagggcaaatgggataccattccagaagaagatcttgatgtaccagaagatgaggaagaccct
gcacatagactggctgagctggaagtgactctgcctcaccagctacttcaggatgtcgatattgtggtttccccctgccgtagctcccaggcagtatcca
tgactctagaggactatgttgaccacgttcagtcaattgttgtatatgcagtatgcgaggaaagattgtcccagcaggatgaagaa
3' EST: CX908129 [+]tttttttttttttttagaacctaaccctggagccactattggttatccaaagtaatcactccactttctgtatcttccagtgagaacaaggagctggctt
actaactagagcagatgtttctgaaagatgcaaccagcttagaaaacaaccacattcttttccaagcctccgcgccatccttgagaaaagaaagatggag
aagatcttaacactgcatttattaatagcttatatttggaagtactggaccatgaaattccatcttcacttctttcagtctgcatcccaaatcaaaaagg
cagtacagagatctgtgtctccatggcagtaaatgcggagcagtaggtggtgcttctagtaaccaagaacacatagtaagagcaagatgaagttctttat
catgtagaatcttccaaagctttgccagtttcttgtgcgcggcagagtcggtccatgatgccctgcaacatgggctgcacaatgcccattagtggacgtt
gagaggggtccccattccaacatgcctccattagtttccaacactcttcatcaaagatggccagcctttctgggcgtgcaccttttctgacgttgttcca
cagatggtctttacttgcacatttctcaaaggcttctggaagttttacagatccagagcagatataccaaaaaagaattccaaatgcatatacatccacc
gagttgtcatattttccagagaaaagctcaggtgccatatgtattggtgtccccacaatgctgccagacatcattgcttctggtttgcaaaatccaagat
ctgtaattttagctctgtgttttt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: