XB-CLONE-3256768
Sequence:
Full length sequence: BC123942 [+]
GAGGTGCGTGAGGGAGGGTGCGGTCCTCAGATTGTAGTTCTGTCTGCTCTGTGTAAGCGGAGTCGCAGGAGTTACTGCTGCCTGGAGCTTTAATAAAACC
CACTGAGTTCTTGATTCTAAAGGTTCGACAATCGCTCTGCTGTTCTGGCAAAATGGCAAATAATGTGAACTGGATTCAGCGTTCAAGTGTGCCAAGTTTT
GCACAGATGCTAAAGAAGAATTTACCTGCTCAAGCTCCAGCTGTGAATCTGAGCTCCACAGTTTCCACATGCTGCCCATATGTTGAACCTGATTGTGGAA
CAGCAAAGGTTACTCAAATCACAGGTTTTCCACCGATTACTTTACCTGAACCATTTTTATCAGAAATGATTGTACCTGCATCACTCTCCTCACTGCCACC
TAGAAAGGTTACAAAGGAATTTGTGGTGAAATACAGACATGGTGACCTCAATCCTGTTTCTGCTTTATACCAGTTTGCACAGATGCAGCGAATGGAAATT
GATCTAAAATCAACTGTAACAACAGTGACAAAACAATGTTTTTCGCTTGAATAAGGAAATGTCTTTGGTGCTTATTTTGCATTCTGTGCTGTTATTGATG
GAATTGAATACAAGCCTGGAATGAGGCAAAAGCAAATGCTACAAAACGTGCACTTGATGAACTTCTTCTACATGAAGATTCTGAGTTAACAGATAAGCGC
ACTTTTATTCATGAAAAGATTTCCTCTATTATTAAAGAAACCTTTACTAATTTAATATCCAAATATCCTGAATATGAAAGCTGTGGTAGCTCATTAGCTG
CGTTTGTCATTGAAAAAGGCATTGCTCTACTATGGTTTATACAGTCTGGCCTACCTCAAGCAAAGTCGGATGGCTGACAGAGAGCACACTGCAACCACAG
GAAGCACTACACAGTCTTTCAGCTGTGTCCAGCACTCAAAGAAAGTAACCTGCTTGGCCCTAGCCAGGTTAACAGTGGGCACATGTGAACCTGGACCCTG
ATCATGAACCCCTAGCAATGAAATAGTAATAAGCTTGGATATTGGATCTTTAGACTTTAGCAAGGAAACTCTTCCATGCTAATGGGAAGTACTGCATGGA
ACCACAGTGGAGCTATATTAAAGTTACAGTTAGAAAAAAGTGACAAAAAAAGAAAATATGGGGGCACTCTGGAAATCCAAAAGACAGGCCATTTGAAAAG
TTAAAAGGAATGTTATTATAGATACTTTTATAGGCAGTTGCTGCTTTTCTACAGTGGAAGTAATGGAATGATGGACAAGTCCATATTTTGCACAGAACCT
GCAACCAACCTTCTTGCTCTAAAACCAAATTTCAATATCTTCCTGTACATGAATCAGCTTCGAAAAGGTGCTGCTCAGACAAATCCCCAACTTTTCCTAC
CTCTGGGGCCATGTACCAGCATGTTCCAGTTTTCCCACGGATGGTCTTGTGGCCAAAAACGCCTTCTGCTGCGATGCCGAAATCAGCGATTTTCATGTGT
CCGTCGCTGGTAAGCAGGATGTTGTCTGGCTTAAGATCTCTGCAAGAGAAGGATAATGGTGCTTGATGCCCAGCAGATTGCTGAGGCTGCCTCCACCAGC
ATACTCCATGACCAGAAAGGCATATTGCTGAGTGCAGAGACAAAAGGGAAGAGAGTGAAACACAGGAACAAGAAACCCCAAACTATTGCATGTAATACAG
GCATGTCCCTCAGAAAAACTAGATACAGAAAGAACAAGTATGTTGTGTAATACTCTTACATGGCTTTTGTTGCTCATATCATGGCATTTAGCTGAGCTTT
GGGGATAGGCAACTAGTAAACCAAATGGTATGGCAGCATTGGAAGTAACTTACTTTGGTCTGAAAGGCTGCAAGGCCACGGCACAAGAATGGACTGCCGC
AAGCAACCTTCAGGATTCTGGCCTCCTTCTCTATCGTTTTCTTCTCCTCGTCCTTAGGTGTCTTCCTGATGACTTTTACAGCCACGTGTTCTTTCTTCGT
GGGGATGGAAGCCAAAAACACCTGAAAAGTCAAAAAAGAATAATGTGCATCTTCCAAAGGGCTAATTGATTAAAGGTTCTGTGCACTTTAACATGCATGC
AAGTTGATAAATAGTGGGCACAGAATAATGAAGAATGGACATCATGGCACCACAAGAGGCAGCACTGGAGTGGATGGTTATTAAATTTGCTTTAGTTGCA
CCTTGAAGCTGCTGTGGGCTTTTAATGGGCCTTATGCACAAGGTGCAAAGCCACACAAGATAACTAATAACATGCATTTAGAAAGCACCAACATACTTGG
TAGTGCTGTACAGTACTTATAGATTGCCTCTCATTGCACCCTGGTGCCACTTTTCATCTCTATAGCAAATGATCCCAGTGAACCCCACTGTAGAAGAATT
TCCATTATCTCCTATTTGTACTGGGCCAAGGATCTTTTTTACCCAAGGTAGGGATAGCTATATAACCCCACATTAACCCACCATAATATTTGCCATGTTT
GGAACAATTAGAGGTTGAAAGAGACCAATTAATACAGAAGCATAAGGAGAAAAAAGCTAACCCGATGAGCTGCTCATATATTTTGCTGCTGTTCATACCA
AAATAGACTTAGGAGTATATTTATTATACTGTGTAAAAAGTGGAGTAAAACATTACTGGTGATGTTGCTCATTGCAGCCAGTCAGATGCTTTGCTTTTTT
AACCTTGAGCAACATCACCAGTAATGCTTCATTTCACTTATTACCCCTTATTGTTTGAAACTGTGCTTACCTTACTTTTTTTTTTTTTTTAAAAACTTGC
CCTTTATGCAATGTCTTTTGTCACCTACCCATAGTTCCATACCTAGCCCTGCCCCCCATTTCGATTAGTAGAGTTTTTGTGTCTGTACATGTAATATTTA
CAACATAAAATTTTTCAGTTATTAAACTACAAGTTAACATCCAAAAAAAAAAAAAAA
5' EST: CX908364 [+]gtgcgtgagggagggtgcggtcctcagattgtagttctgtctgctctgtgtaagcggagtcgcaggagttactgctgcctggagctttaataaaacccac
tgagttcttgattctaaaggttcgacaatcgctctgctgttctggcaaaatggcaaataatgtgaactggattcagcgttcaagtgtgccaagttttgca
cagatgctaaagaagaatttacctgctcaagctccagctgtgaatctgagctccacagtttccacatgctgcccatatgttgaacctgattgtggaacag
caaaggttactcaaatcacaggttttccaccgattactttacctgaaccatttttatcagaaatgattgtacctgcatcactctcctcactgccacctag
aaaggttacaaaggaatttgtggtgaaatacagacatggtgacctcaatcctgtttctgctttataccagtttgcacagatgcagcgaatggaaattgat
ctaaaatcaactgtaacaacagtgacaaaacaatgtttttcgcttgaataaggaaatgtctttggtgcttattttgcattctgtgctgttattgatggaa
ttgaatacaagcctggaatgaggcaaaagcaaatgctacaaaacgtgcacttgatgaacttcttctacatgaagattctgagttaacagataagcgcact
tttattcatgaaaagatttcctctattattaaagaaacctttactaatttaatatccaaatatcctgaatatgaaagctgtggtagctcattagctgcgt
ttgtcatt
3' EST: CX908363 [+]tttttttttttttttggatgttaacttgtagtttaataactgaaaaattttatgttgtaaatattacatgtacagacacaaaaactctactaatcgaaat
ggggggcagggctaggtatggaactatgggtaggtgacaaaagacattgcataaagggcaagtttttaaaaaaaaaaaaaaagtaaggtaagcacagttt
caaacaataaggggtaataagtgaaatgaagcattactggtgatgttgctcaaggttaaaaaagcaaagcatctgactggctgcaatgagcaacatcacc
agtaatgttttactccactttttacacagtataataaatatactcctaagtctattttggtatgaacagcagcaaaatatatgagcagctcatcgggtta
gcttttttctccttatgcttctgtattaattggtctctttcaacctctaattgttccaaacatggcaaatattatggtgggttaatgtggggttatatag
ctatccctaccttgggtaaaaaagatccttggcccagtacaaataggagataatggaaattcttctacagtggggttcactgggatcatttgctatagag
atgaaaagtggcaccagggtgcaatgagaggcaatctataagtactgtacagcactaccaagtatgttggtgctttctaaatgcatgttattagttatct
tgtgtggctttgcaccttgtgcataaggcccattaaaagcccacagcagcttcaaggtgcaactaaagcaaatttaataaccatccactccagtgctgcc
tc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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