XB-CLONE-3256698
Clone Name: IMAGE:7687171
|
Gene:
plekha4
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX908479 [+]
aaagaaggccggcgctggtcaagcaaagactcaaccaggctacacccagccctgagagaaagcctgattccgtgcctgagctcctagaagttaaaaagaa
ggcagtggaaagagctaaaacagcaacgacaaagaccagccctgccacgtcccctggttgcagcagacactcggatgacgattacaaaatggccaattcc
gggcagagggaacgtattatcagcctttcctacacactggccagcgaggcctcagaactcagtaaactgatgacagccaaaacattagccgatgaaatgg
atgcaaacagcgacgtctccacatccgatgaggcatatccctgggatttcattatgcaggattcccgctccagcaaacacccagaaacgagtgagcgctg
ccagctcaaaggcccccctgatgcaccccaaggggaggacaggaatgccaatgtggcagaaaactgccagacttattgctcaacaaaccaatgctcatcc
agcggtccctgcagccctccgctacagcaccgcgactcccccaagggccacacagacaatcagcactatgagaactggccggcaccgagagaaaaaggaa
gcacggataactgcccgaaaatcccacaggaatccacagtgttgtccaaatgcaacggccaagtggccaactacgagttcctcatacaggacctgcatta
taacccgctgaatatcagcaaggtgacagactattgtaaggaagatagggagccaatcaggatcacccttcttcagtccagcttctaggagcaatataag
gactctatggaccgagctatgtctgctgtaggcagatcggg
3' EST: CX908478 [+]aaaactttctctttaccacattcaggctcatactggaagcagtgttcctggcccgatctgcctacagcagacatagctcggtccatagagtccttatatt
gctcctagaagctggactgaagaagggtgatcctgattggctccctatcttccttacaatagtctgtcaccttgctgatattcagcgggttataatgcag
gtcctgtatgaggaactcgtagttggccacttggccgttgcatttggacaacactgtggattcctgtgggattttcgggcagttatccgtgcttcctttt
tctctcggtgccggccagttctcatagtgctgattgtctgtgtggcccttgggggagtcgcggtgctgtagcggagggctgcagggaccgctggatgagc
attggtttgttgagcaataagtctggcagttttctgccacattggcattcctgtcctccccttggggtgcatcaggggggcctttgagctggcagcgctc
actcgtttctgggtgtttgctggagcgggaatcctgcataatgaaatcccagggatatgcctcatcggatgtggagacgtcgctgtttgcatccatttca
tcggctaatgttttggctgtcatcagtttactgagttctgaggcctcgctggccagtgtgtaggaaaggctgataatacgttccctctgcccggaattgg
ccattttgtaatcgtcatccgagtgtctgctgcaaccaggggacgtggcagggctggtctttgtcgttgctgttttagctctttccactgccttcttttt
aacttctaggagctcaggcacggaatcaggctttctc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: