XB-CLONE-3255775
Clone Name: IMAGE:7687726
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Gene:
gramd1c
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121880 [+]
CAGATGTTTAAACAGCTTGTATCATGCCCATGTTGTTGCCCTCTTGCCCTAACCCAGTTCCTTCCCCAGATACTGAGGCAATCGCATGTAGAAGAAAGTC
AGGGGGAGAAGGCAGGGGGCAAGAGCCCCCAGTTAGGCGCAGACAGAAAGCCCAGCCTCCGGGCAACCATCGGAACCCCTGCTCTGGCACTGGACTTGAA
TGGGAATGAAAACCAGAATTTAGACCGGAGCAGCTTTTCTGATACAGGGGATGAGGAAGATACCAAGTACCCCGAGGAAGGGATCGACGGGCGGCTCTAC
ATTAACCGCGTGTATCAGATCAGCGCCGAGAGGATGTTCCAGCTTCTCTTCACCCAATCACACTTCTTTGTCAACTTCCTGGGGTCCCGCAAAGTGTTTG
ACCTGGAATGTACCCCCTGGCAGTCAGACGGCAACGGGAAGCAGACAAGGACCCTCACTTACACCATCACCATTAACAACCCGCTGGTCGGAAAGTTCAC
CACGGCCACAGAGAAACAGGTGTTGTACAAAGGCAACCAAGGTGGGCTGAGCTACTCGGTGGAAGCGGAGGTTCTCACACACGACGTGCCTTATCACGAC
TACTTCTACACCGTGAATAAATACAGCATTGTCCGAACGGCCCAAGACAAATGCAGGTTACGGGTCTGCACGGACGTCAGGTACAAAAAGCAGCCCTGGG
GCCTGGTCAAGACTTTCATTGAGAGGAACTCGTGGAGTGGCCTGGAGGATTACTTCAAGCAGATGGAGTCGGATCTGATGATAGAAGAAAACGCTTCAGG
TTCCGTCTTAGCAGAGGCCGGAAAATCCATGAGAAGGAGGAGACGGAATCCGAACCGCAGCCTGGGGGAGCACCTTCCCAAACATGGCGCCCAGCACGAT
CCCACACACATGGGACTGGATCTGAGGAAGGATCATACAGGCAGGAAAAGTGCCGGGGGAAGGAACACTACAATCATACTAGTAATGAGTATCTTCCTGG
TTCTGTTGGTTCTGCTCAACGTCACCCTGTTCCTAAAGCTGTCTAAGATTGAGCATGCAGCCCAGAGCTTCCACCATTTTCACCACAAGCAGGAGCGCTA
TGCCAGCCTACACCCCGGTGGGATTGGTGGTGCGGATCAACCCCACAAAGAGAAGGTATTCGGCCCAGGGTTAAGGGGGCTACTTCAGGACTCCATTTCA
ACCATTGAGCAGCTGAAGAACTCCCTGCTCCTCCTCCAGAAAAGCTTTGATCTGCTCAACAGGACGCGGGAAGAGGACCCTGCTGGGAGTTAGCACCCTG
ACGTGACTTTATGGGTGGGGGGTAAATGTTCCATGAAGGACCCAGCTGGGAGTTAGCACCCTGATGTGACTTTATGGGTGGGGGGTAAATGTTCCATGAA
GGACCCAGCTGGGAGTTAGCACCCTGATGTGACTTTATGGGTGGGGGGTAAATGTTCCATGAAGGACCCAGCTGGGAGTTAGCACCCTGACGTGACTTTA
TGGGTGGGTGGGGGGGAGAAACGTTCCATGAAGGAAATCTTAGTACCGGAGAACTGGTTACAGAGACTGGGAAAGTAACTCAGGAGTGGCCCTAAGGGCT
ACCAACCTTGGAGCAAAGGGCCACATTAACCCCTTCAGTTCCTGCTCGCCGCTGGCGTCGCCCCCCTTCTTCCATTTTATTTAAATCCTACGTTGTTTTT
GTGAATTGTAATTCTGTTTCTTGTTTGTCCTGTTTTTTTCCCCTGAAGTTTCTAAAGGTTCTCAGGTTCCTGCCCAATCCGGTGCCGTTATAAAGCAATG
TTTATTGCTGGGGGGCTTCTGGGTGTTTATAGTTTGCACTAAATACATGGGGGGCAGATTTACATTGCTGCTCTAATGGGGGGGGGGGGTCACTGCTTCC
TGTTCTACCTCTTACTCTTCCCAAATTCCGTTATTTTATTAGGGAATATTTAGCTCACAATAGCTAATGCTTTGCCCGTTTGCGCCCCCTGGCTGTTGCT
TATGGTAGTGCTGTCTGTGCCACCCCCAATCTGCCCCGCTGGCAGCCCTTAGATTCACTGTATGTGAGAGACTGGCATTGCCCCCTGCCCAGGCCTCTCC
TTTTAAGTTGGTGAAACTGTCATTTTATCCGTAGCCTAGAACCATTTGCATTTGGGCCATTTGCCCCCTCCTGTGTCAGTCTCTGAGCTACAGAGCCTAT
TGGGCCCCTTCAGAACCCAACTCCCCAGGGTGCCATGGCATTGGGCCGGTTCTCCTGTTACAGTGAAATGTTACAGCAACAAGACATGGATTTCTGGTTT
TCTTTTGTATTTTATTCCTTTGGGGTTTAGGCTTTTTTTTATTAAGATTCTAATGCCCCAATATACGTGAAGGAGGGGTATTTATTGCATAGTGTGTGTG
TGTGTGCTGATTATTTATTGTGTTTTTGGTGCCTTGTACCAGAGTCTGTGTCTGTATAAACTACAACTCCCAGCAGCCCCTGCTCCAGCTTTGTGCCATG
GAGGGATGTTGGGGCTTGTGGGCCAATAGAAATCATTTACCAGATTTTACACAATGGATTGTTCCATTGACAGTACATCTCCCCCTGAATGGTTTAATCT
AACGAAACGGGACAGGTGCAACGTGGCACTTTCCCAATGCCCATTACTGTGCCCACTTCATCCTAAAACTCCCGCCCCCGGACTGTGCTTATTGGCTGCC
CATATCTGATCTAGGGTTGGGCGGCTGTGATGACTCTGGGTCGCACCCACTCCTTTCTGGAGCCCCCACTTTCTTTCCTTAGGGGCCAATTTGGCTTCAC
TGCCCCAAAATTCATTTAGAAGCAATTATGAAATGATGGGATCTGTTATCCAGAAAGCCCTGATATGTGCCAGTGCTGTTCCCCATAATGCCCTGTTTAA
CTCATTCATTTCTGACTGATTCGCTTTTTCCCTGTAATAATGAGACAGAACCTTGTGCCTGATGGGAACGGAGCTGCCGGAATCCATGTTGGTGGCACAA
ATCCTATTGGGTTTCACTAATATTTATTAGCCTTGGGGTACAGCGCCCCAAATGCTGGGAACCCCCAATAGGATGCTTGCCTAGCAACAGGGTCATGTGA
CCACTTTGGCCTTTTTCACAGAGGCCTGAGGATTTTCTTGTGTAAAAAATCAATAAAATCGGGAACTGAAAAAAAAAAAAAAA
5' EST: CX910080 [+]cagatgtttaaacagcttgtatcatgcccatgttgttgccctcttgccctaacccagttccttccccagatactgaggcaatcgcatgtagaagaaagtc
agggggagaaggcagggggcaagagcccccagttaggcgcagacagaaagcccagcctccgggcaaccatcggaacccctgctctggcactggacttgaa
tgggaatgaaaaccagaatttagaccggagcagcttttctgatacaggggatgaggaagataccaagtaccccgaggaagggatcgacgggcggctctac
attaaccgcgtgtatcagatcagcgccgagaggatgttccagcttctcttcacccaatcacacttctttgtcaacttcctggggtcccgcaaagtgtttg
acctggaatgtaccccctggcagtcagacggcaacgggaagcagacaaggaccctcacttacaccatcaccattaacaacccgctggtcggaaagttcac
cacggccacagagaaacaggtgttgtacaaaggcaaccaaggtgggctgagctactcggtggaagcggaggttctcacacacgacgtgccttatcacgac
tacttctacaccgtgaataaatacagcattgtccgaacggcccaagacaaatgcaggttacgggtctgcacggacgtcaggtacaaaaagcagccctggg
gcctggtcaagactttcattgagaggaactcgtggagtggcctggaggattacttncagcagatggagtcggatctgatgatagaagaaaacgcttcagg
ttccgtcttagcagaggccgggaaaatcatgagaagga
3' EST: CX910079 [+]tttttttttttttttcagttcccgattttattgattttttacacaagaaaatcctcaggcctctgtgaaaaaggccaaagtggtcacatgaccctgttgc
taggcaagcatcctattgggggttcccagcatttggggcgctgtaccccaaggctaataaatattagtgaaacccaataggatttgtgccaccaacatgg
attccggcagctccgttcccatcaggcacaaggttctgtctcattattacagggaaaaagcgaatcagtcagaaatgaatgagttaaacagggcattatg
gggaacagcactggcacatatcagggctttctggataacagatcccatcatttcataattgcttctaaatgaattttggggcagtgaagccaaattggcc
cctaaggaaagaaagtgggggctccagaaaggagtgggtgcgacccagagtcatcacagccgcccaaccctagatcagatatgggcagccaataagcaca
gtccgggggcgggagttttaggatgaagtgggcacagtaatgggcattgggaaagtgccacgttgcacctgtcccgtttcgttagattaaaccattcagg
gggagatgtactgtcaatggaacaatccattgtgtaaaatctggtaaatgatttctattggcccacaagccccaacatccctccatggcacaaagctgga
gcaggggctgctgggagttgtagtttatacagacacagactctggtacaggcaccaaaaacacaataaataatccg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: