XB-CLONE-3255759
Clone Name: IMAGE:7687824
|
Gene:
MGC145518
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC121881 [+]
CAGCCTGGCTACAACCGAGATGTGAGGGAGCGAAGGAGCATATTGGTAGTTGTGTCCTGCTGCTGCCTGCATCTCCCTTCCCCTGTGTCCCTGGCATTGT
CCCTCCCCTGCACTCACATCTCCAGCCTGGGCTACTGGGTATCCGACATGCCGACCTGCATAGTGAAGGGATGTCTGCACAGCTGCAAGCGGAAGAAGCA
GTTCCCGCACGTCGTCCTTCACGTTTTTCCCGGAAATCTCACCATAATAAAGAACTGGCTGCAACACATTCCCCAGAATTTCGGAGACATCAACGAGATG
GCACTTAAAATTTTAGAAGGAAAAAAAAGCGACACCTTCCGTCTGTGTTCGGAACATTTCACCCCGGATAGTTACACGTACCAGGGCACCAAACGGGTTC
TGAAAAAAGAGGCGGTGCCGACGCTCTTCCCCGAAACCTTTTCATCTGCGTTCGTCAATCTGAGTGCGCAACGATTTAAAAAGAAAAAGGGGAAAGATGC
GGAGTCAGAACCTGACTTGCAGTCCATATCATCACTTCTGTCCATCGCCACTTTCCCATATTCCTTAACTTCGTCTCTGCTTCAGGCCCCGCACAAACCA
GAAGATGCTTCCGGAACTTATTCATCAGCTTTGCTACCGTCTCCGCACCCAGAAACTTCTCTTCTGGAGCCGACCGAGACATCTGCGGAAACCTCCTCCC
AGTCAAATCCGCCGACGGAGGAAAGATCATCTGGACTACCACAACCTTCCTGTGCTCATTGCAACAAATCTATCCCGAAAAGCGCAATATTTGTCGACAC
CGACATGAACACTGATTATTTTTATAGGAAAAAGAGTGTGTCGACCAATACGTTCACTAAATTTGGTAAGAAGAGTGTCGCGTCACAGAAGATGATTTTT
CAGAAAAATAAGGGTTTGCAGTGTAAACTCATTACGGGTCAGTACCAAGTCCCAAGAATCCAGTCTTTGTCAACTCTACTAAACTCAGCTGGCACTCAGG
CCCACTCCACAAACAGCTATAATGGGCCGGCTCTGGATATGAGTTCAACCTGTCTGAGAATGAGAGAGGAAGCAGAGTCCGATTCCGTTGGTATAAAGGG
TGTAGATATGCGCGTGGGTGCTACAGAACACATAGACCTGACCTATGAACCTTTAGTTCAGAACACATACAATGCCAAAGTTGCCGATGATGAATGCGAA
GTGGAATCTACAGTTGATTCAGAGAATCTGACGGATTTGGGATACAAAGATGAGGTTGATGAGAACAAGTTTCTTGTATTCGAGTCTTGTCTGGATAAAC
TTCTGTGGGGCTCACGTTGCAGGGGAGGGAGGAACTGTCCGGCAGCCATTAAGAGCATTCGGAAGAAAATAACCGGATCTTTCCTAAGTGTAAATGCTGT
TTGTGCCAAAGGACATTCATTCCATCTTTGGGACAGTCAACCCAAAAAGGGTCACATGCCGATCGGCGATGTGTTAATGTCGGCCGCCATCGTACTAAGC
GGCTCCAGTTACAGCAAAGTGAGTTACATGAATAAAATTCTGGGGCTCAGGCAGATTGGGAAGGAAACCCATTATCGCCACCAAAGACTCTTCCTGTTCC
CGACCATCAATCACCACTGGGAATTGGAGCAGCACCGAGTGATGAAAGAGTTGGGGCAGAAACCAGTGTGCCTCGTCGCTGATAGGAATTGCCACAGTGC
AGAAAAGCGCTGTGTTTGCACATTTATGGAGGCAACCTCAAAGAAAATTGTGAGCTTGAAGGTGGATCAAGCGTCGCCTGAAACATCTTCCCTCGCACTC
GAGAGACAAACATTCCAAAGCGCCTTGGATAACGTGCTGCAGTGCAACGTAAATGTTCAGCTGGTCTGTACGGAGCCGCGGCCCTGGGTAAAGAACCTGA
TGGAAGAGAATTATGGGCACATCCTACATGAGTTTGATGCCTGGCGCTACGGGAAATCCGTTGGTAACAGAGTGGCTGCCGCCAGCAAAAAGAGAAACTG
TTCCGAGCTTGCCCAGTGGAGCGCCCCAGTGAGAAACCATTTATGGTGGGCGGTTAGATCATGTGAAGGCAACACGGAACTAATGATGGAGAAGTGGAAC
TCACTAATATATCACGTAATTGGGGTACATAAATGGAGGACGGGTGCGCTCTATCACGGCTGTGAACATGCGCCTCAGGAGGATGAAGTGACAAGATCGT
TTCTAGGGAAAGGTTCTAGAGCTCATAGAAAGCTGAGAGAGATTGTACTGAGTCGGGACATCAACAAAGATCTCCCAAAGCTGCGGAATATTTGTCACAT
GGGGGAGCTGGATGTGTTCCGCAGCAACGCCCTCAAGTACCGGCCAAAGCAAAGCCACTTCTCTACCCATTCCTTAATTGCACGGACTCAGCTGGCCGCA
CTAGACCACAATAGGAATGTCCATCACCAGCTGGCCATCTTGGCTAATGGAAGAGATGAGGAAGTGCTTAGGGAGGACTATCCCAGGGTGGAATTCCGTG
AGTTGAAGAAGGACTGGAGAGTGAAACCTGTCTATGACTTGGCGCGGTGTCATTTTATATTCACTATCATGAAAGACGTGGTCAGATACGTGTATGGGGA
AAAGTGTCCTGGGTAGCGGCGACTCCCTTCTGAATCCAAACCCAACAGGGAGAGGGCCACATACAGAATAAAGATCTTCTCAAGGGTCGCCAGTCACATG
ACTGATATCTGTAGATCACATCGATCTGTGTTTCATTAACTTTTGCCAAATGAGGGAACATCCAATAAAAATGCAGCTATTTCTTATAGATATAAATGTG
TATATAGAGTTTAGAATAAATATCCTGGAAAATGAAAAAAAAAAAAAAA
5' EST: CX910099 [+]ctggctacaaccgagatgtgagggagcgaaggagcatattggtagttgtgtcctgctgctgcctgcatctcccttcccctgtgtccctggcattgtccct
cccctgcactcacatctccagcctgggctactgggtatccgacatgccgacctgcatagtgaagggatgtctgcacagctgcaagcggaagaagcagttc
ccgcacgtcgtccttcacgtttttcccggaaatctcaccataataaagaactggctgcaacacattccccagaatttcggagacatcaacgagatggcac
ttaaaattttagaaggaaaaaaaagcgacaccttccgtctgtgttcggaacatttcaccccggatagttacacgtaccagggcaccaaacgggttctgaa
aaaagaggcggtgccgacgctcttccccgaaaccttttcatctgcgttcgtcaatctgagtgcgcaacgatttaaaaagaaaaaggggaaagatgcggag
tcagaacctgacttgcagtccatatcatcacttctgtccatcgccactttcccatattccttaacttcgtctctgcttcaggccccgcacaaaccagaag
atgcttccggaacttattcatcagctttgctaccgtctccgcacccagaaacttctcttctggagccgaccgagacatctgcggaaacctcctcccagtc
aaatccgccgacggagganagatcatctggactaccacaaccttcctgtgctcattgcaacaaatctatcccgaaaagcgcaatatttgtcgacaccgac
atgaacactgattatttttataggaaaaagagtgtgtc
3' EST: CX910098 [+]ccaggatatttattctaaactctatatacacatttatatctataagaaatagctgcatttttattggatgttccctcatttggcaaaagttaatgaaaca
cagatcgatgtgatctacagatatcagtcatgtgactggcgacccttgagaagatctttattctgtatgtggccctctccctgttgggtttggattcaga
agggagtcgccgctacccaggacacttttccccatacacgtatctgaccacgtctttcatgatagtgaatataaaatgacaccgcgccaagtcatagaca
ggtttcactctccagtccttcttcaactcacggaattccaccctgggatagtcctccctaagcacttcctcatctcttccattagccaagatggccagct
ggtgatggacattcctattgtggtctagtgcggccagctgagtccgtgcaattaaggaatgggtagagaagtggctttgctttggccggtacttgagggc
gttgctgcggaacacatccagctcccccatgtgacaaatattccgcagctttgggagatctttgttgatgtcccgactcagtacaatctctctcagcttt
ctatgagctctagaacctttccctagaaacgatcttgtcacttcatcctcctgaggcgcatgttcacagccgtgatagagcgcacccgtcctccatttat
gtaccccaattacgtgatatattagtgagttccac
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: