XB-CLONE-3255453
Clone Name: IMAGE:7688271
|
Gene:
sf1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX910580 [+]
caaggaaagaggaggagtttggaagtattgtgacaaagatagaccgcgtgatggatttctcaaggaaacgtaagcgcagtcgatggaatgatgaaactcc
agaccagaaaactattattcctgggatgcctacagtaattcctccagggcttactcgtgaacaggaaagagcttatatagttcaacttcaaattgaggat
ctgacgcgcaagcttcgcacaggagacctgggaatcccccctaacccagaagacaggtctccatcaccagaaccaatttataacagtgaagggaaacggc
tgaacacacgagagttcagaacgcgcaaaaagctggaagaggaacgtcataacctcatcactgaaatggtgggattaaaccctgattttaagccacctgc
agattacaaaccaccagctacccgtgttagtgataaggtcatgatccctcaggacgagtacccagagatcaactttgtagggttgcttattgggcccagg
ggaaatacattgaagaacattgaaaaggagtgcaatgccaagatcatgatacggggtaagggttccgtaaaggagggcaaggttggcagaaaagatggac
agatgcttcctggagaagacgagcctctacatgccttggtaacagctaacactatggaaaatgttaagaaagcagttgaccagattcgtaatatcctaaa
gcagggcatagaaactccagaagatcaaaatgatttacgaaaaatgcagctacgagagcttgctaggctgaatgggacactgcgagaagatgacaacagg
atactgaggccatggcagagctctgaaactagaagcatcaccaacactacagtatgcacaaa
3' EST: CX910579 [+]aaaaaatgtcttttaatttaggacaggcccttattacagaataatcagtcacaaaaaaataagcaagcatcgggggcacagaaatgtgttcccttagctt
aacagtacacctggcccctccctcaagaggatcttaaaaatggtgagttatttacattaaagtctccttccccaaaggcaaaaatgatgctgggggcatt
agcaagtatttaacaatcaaagcaacaactcactaaagtcgtaatgtacctctcaactacgcagttaaatacaaacagtatctaagaattggcaggggga
ctagaaatttattccaagttagagagcacagtgcctatgatataggaaggcagttttagatctactgtgaaatactgaataactgtggcatgagcgtgag
aagtgaagtgtattttatgataaagattttgcaaattagcaacattaaacttccaaattacatataacatctacattttcacactaaatgttttccatta
tggcatagtttttcataaaaggctgctagatctggttagccttctggatgcaatagtgctgcacaggtaatgatgcaacctacctagaacaaggaactaa
aaatgttagataattctcagtagaaccgtgtatttgattgcatatccatacttatttctacattaagtccttaacaacgttcccttgatattgctgtacc
tagagaatgaaaatttttcttatacatatatatatatgtatatacttttatatgaaagcatgatgtattaccnagatttc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: