XB-CLONE-3255343
Sequence:
Full length sequence: BC161080 [+]
gtctgctctgtgtaagcggagtcgcaggagttactgctgcctggagctttaataaaacccactgagttcttgattctaaaggttcgacaatcgctctgct
gttctggcaaaatggcaaataatgtgaactggattcagcattcaagtgtgccaagttttgcacagatgctaaagaagaatttacctgctcaagctccagc
tgtgaatctgagctccacagtttccacatgctgcccatatgttgaacctgattgtggaacagcaaaggttactcaaatcacaggtaactttcgagaacca
tttttatcagaaatggttgtacctgcatcactctcctcactgccacctagaaaggttacaaaagaatttgtggtgaaatacagacgtggtgacctcaatc
ctgtttctgctttataccagtttgcatagatgcagcgaatggaaattgatctaaaatcaactgtaacagataagcgcacttttattcatgaaaagatttc
ctctattattaaagaaacctttactaatttaatatccaaatatcctgaatatgaaagctgtggtagctcattagttgcgtttgtgattgaaaaagatact
tttataggcagttgctgcttttctacagtggaaataatggaatgatggacaagtccatattttgcacagaacctgcaaccaaccttcttgctgttaaacc
aaatttcaatatcttcctgtacatgaatcagcttccaaaaggagctgctcagacaaatccccaactatggtgcttgatgcccagcagattgctgaggctg
cctccgcctgcatactccatgacgagaaaggcatattgctgagtgcagagacaaaaggagctatgacagaagctgtagaagtttggaccaagactctccc
tctaaaaagaaaaaaatgcaagctaattattcctcaacaactcacttacttgtggggaagaaagtgccatcctcactccaaaccaaaccaagtgacttgg
aaactacagtgttttatattcctggggttgatgtaaagttacactacaattccaaaacattaaagacagagtcgccaaatgcctcaagaggatcttcttt
gccaagaactctttcaaaagagtccaagctgtatgccaaaggaaagggaagcagtggagttaaaacagcaaaattgtatgcttgggtagctctgcagtca
cttccagaagaaatggttatcagcccaaacctgctagatttccttgagaaagccttagaaacaataccaatcactccagttgaaaggaactatgcagcag
tgagctctcaagatgaggatatcggacagtttgacctgcaggatccactggaagaatcaacaacctctttagtatcatcttcaacttctccatattcatc
ttttcctgttgatgtggttgtctacgtcagggttcagccatctcaaattaggtttagctgtctgccagtttcccgggttgaatgtatgctgaaattgccc
tctctagacttggtattctcttcaaacagaagtgagctagaaacccttggaccatcacacccctcagattcatctattggaaatctttccatgccaagtg
gatcaaaaccaaatctaaacaaatctgggatgccagggacatccccggggcttggtagcccccttgggagaacacgacacagcagcagccagtctgattt
gaccagctctagcagcaactcgtcaggcctgagtttcacagcttgcatgtccgatttctccctgtatgtgtttcatccttatggagcaggaaaacagaaa
tctgcagtatcaggactcactccaggacccagtggcttaggtcaggtagatgaagaacctacgtcagttactggccgtaaagactctcttagtatcaatc
tagagtttgttaaggtcagtctgtccagaatgagacgatcaggtggagcttcattctttgagagtgtatctgctaacaaaacccctagtaagatggatac
cactttaatcaacatatcagcggtgtgtgacatcggatctgcctctttcaaatatgatatgcgccgccttagtgagattctggcttttccaagggcatgg
tacagaagaagtattgctagacatttgtttcttggtgatcaaaccatcaatttacctgaactaaatttacttcggaatgttgatgccaacaatgcagaaa
gcagcacagcagtgaagaactcgagtttgttaagtggcttcaggggaggctccagttacaaccacgagacagagacaatctttgctttgccaaggatgca
gctagacttcaaatctatacacgttcaggagcctcaagaacctgtcctgcaagatgacagcaaacctaaggtggaatgcagtgtgttaacggagtttact
gaccatatctgtgtgacgatggatgcagaactgatcatattcctacatgatatggtgtctgcttacctaaaggagaaagaaaaagttaaatttccacctc
gagtctttactactcgcccaggccagaaaagccctgttatacagatcaatgaaagctcttctgagaaggagaaagaagaaaacactaactacacctatgt
agactggagggaatttatgtgcaatacttggcatcttgagcccaccctcaggcttatttcatggacaggaaggaaaattgacccagttggtgttgattac
atccttcagaagttaggctttcaccacgccagaactactattccaaaatggttacagcgtggagtaatggatcctctggataaggtcctttcagtcctca
taaagaaacttggctctgcccttcaagatgaaaaagaaaagaaggggaaggacaaagaagagcactgaacacaaaagcaagaaagtgaacaacatgttaa
actactgaacaaacacatttttagtatcttttgtatagcaaatcaatgtttttatttgacatgagcccatttgactttggaaacaagtttttgatttcca
gatcaaccaccaatattgagattggattctctggattcaaactaattcactgaaaatgtgacaaacttaacttctgtctttctttatgctactgtgctac
taaccccttcatccctgggtgctgttctagttgtgttactcttgattgctattccattttttttgttaataagtgagtttacgctttatgccagctgcag
tgttttctataggccaagtgtatagctctctttgctttaagtacctgcatgttggtgagtaagaacatgcattcttgagcaaagtaacactgagatatcc
ttttgtgtaagtaatcttggggatgagcgcctgtattgcaaactcgtaaataggaacctcagggatgaggggggttataataattccgttgtgaataaaa
ttgttccattggggaagcattgcatgtttatgtatattttggactaaaaatagggttgaaacactgtaaaatgtagggggctattgttattactttgtat
taagtgcaatatgattttatttctgtgataactgcagaatattaactgtgaatttattttattttgtcataatacattttgttgacaatatgaaaaaaaa
aaaaaaa
5' EST: CX910752 [+]tctgctctgtgtaagcggagtcgcaggagttactgctgcctggagctttaataaaacccactgagttcttgattctaaaggttcgacaatcgctctgctg
ttctggcaaaatggcaaataatgtgaactggattcagcattcaagtgtgccaagttttgcacagatgctaaagaagaatttacctgctcaagctccagct
gtgaatctgagctccacagtttccacatgctgcccatatgttgaacctgattgtggaacagcaaaggttactcaaatcacaggtaactttcgagaaccat
ttttatcagaaatggttgtacctgcatcactctcctcactgccacctagaaaggttacaaaagaatttgtggtgaaatacagacgtggtgacctcaatcc
tgtttctgctttataccagtttgcatagatgcagcgaatggaaattgatctaaaatcaactgtaacagataagcgcacttttattcatgaaaagatttcc
tctattattaaagaaacctttactaatttaatatccaaatatcctgaatatgaaagctgtggtagctcattagttgcgtttgtgattganaaagatactt
ttataggcagttgctgcttttctacagtggaaataatggaatgatggacaagtccatattttgcacagaaccctgcacca
3' EST: CX910751 [+]tttttttttttttttcatattgtcaacaaaatgtattatgacaaaataaaataaattcacagttaatattctgcagttatcacagaaataaaatcatatt
gcacttaatacaaagtaataacaatagccccctacattttacagtgtttcaaccctatttttagtccaaaatatacataaacatgcaatgcttccccaat
ggaacaattttattcacaacggaattattataacccccctcatccctgaggttcctatttacgagtttgcaatacaggcgctcatccccaagattactta
cacaaaaggatatctcagtgttactttgctcaagaatgcatgttcttactcaccaacatgcaggtacttaaagcaaagagagctatacacttggcctata
gaaaacactgcagctggcataaagcgtaaactcacttattaacaaaaaaaatggaatagcaatcaagagtaacacaactagaacagcacccagggatgaa
ggggttagtagcacagtagcataaagaaagacagaagttaagtttgtcacattttcagtgaattagtttgaatccagagaatccaatctcaatattggtg
gttgatctggaaatcaaaaacttgtttccaaagtcaaatgggctcatgtcaaataaaaacattgatttgctatacaaaagatactaaaaatgtgtttgtt
cagtagtttaacatgttgttcactttcttgcttttgtgttcagggctcttctttgtccttcccttc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: