XB-CLONE-3254805
Clone Name: IMAGE:7688660
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Gene:
eps15l1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC166356 [+]
gcgctcccgcggacggcaggaaagatggcggcgctcatccctctcacacagctttccggtggcaatccattttatgaaacatattacaagcaggtggatc
catcatacattggcagagtaggaccagcagaagctgctctcttcctcaaaaaatctgctctctcagacattatccttgggaagatatgggacctggctga
tccagagggaaaaggttacttggataaacatggattttatgtagcactgaggctggtagcttgcgctcagagcggacatgaagtcagtctatcaagttta
aatgttccagtaccagcacccaaatttcatgacactagcagtcctctgttaatcactccaccccctgttgagggacattggggtgttcggatagaagaga
aatccaaatttgatggtatttttgacagtctggtgccagtgaatggcttgctttctggagacaaggtgaaacctgtacttatgaactccaagctacctct
ggatgtcttaggaagggtgtgggagctcagtgatattgacaaggatggacacttggacagagatgaatttgctgtagcaatgcatcttgtatacagggcc
ttggagaaggaaccagtcccttcagtgttacctccatctctcatcccgccgaacaagaggaagaagactccaacattcccaggggcagtttcagttcttc
cctccagcccaccacctaaagatagtctacggtccaccccatctcatggcagtatgagcagtttaaacagtgctggcagcctttcacctaagcattcctc
caaaccagcccagcctgctgtaaactgggtggtcccattgacagacaaaatgcgttttgatgacatctttctgcagacagatctggacatggatggattt
gtgagtgggctggaagtgaaggatatatttatgcagtctggtctgtctcagaatatcttagcacacatatgggctcttgccgacacaaggcaaacaggca
aactgaacaaggaccaatttgctctagcaatgtattttatacaactgaaggtcagcaaagggattgatcccccacaggtattatcacctgacatgatccc
accgagtgaaaggaacacccctatacaggacagttccagttctgttggctcaggtgaatttactggtgtgaaagaactggatgagatcagtcaggaaata
gcccagttgcaaagagaaaaatatgctctggagcaagacattcgagaaaaggaagaagccattagacaaaagagcacagaagttcaggacctgcaaaatg
atttagaccgggagacctccacccttcaagaactggaagcacaaaagcaagacgcccaggacaggctggatgaaatggaccaacaaaaagcaaaattgaa
ggatatgttaaatgacgtccgacaaaagtgccaagaggaagggcaaatgatttcctcactgaagatccaaatccagtcccaagagtcagatgttaaagca
caggaagaagaacttaacagagcaaaatcggaactaaaccgactccagcaggaggagactcagttggaacaaagtattcaggcaggcagggttcagttag
aaacaataataaagtcattgaaatccacgcaggaagaaataaatcaggctcgaagtaaattatcgcaactgcaggagagccagcaagaactaaatcaaaa
tagtgatcaatataatgatgctttgaatggagaccatggaagcagcatgaacaacctgtctgacacagagtcattttcctcgagagagagaactgaccat
tcttcaacaattgaggacccattcaaaaacaagtcccatctgttcagcaataacaaccaagacatttattcagacccattccagacagaagaccccttca
catctgatccctttaagggagcagacccttttaaaagcaacccctttggagatgacccatttaaggagagtgatccttttcatgccccaacatcagagga
cccattccagaagccagctaaaggagacccattttctgctgaccccttcactaagaacagtgtccattcgatgaccctacccctaaagtctgatccattt
gagagcagcgatcctttcatctcttccacactgccaaagaaaaaaacagatccatttgggactctggacccatttgggagcagcgcattcagcagcaatg
aaagttttgccgattttagtcacatgtccaagcagcaccaagcaccagatcccttttctgctaccttccctggtactggattttctgacgaccccttcaa
aagcaaacacgacactccagcattgcctcctaagaggagtgtacccccaaggccaaaacccccaagtggcaaaagcactccagtaagtcagctcagcaca
tcccacttccagaagtctcatgatccatttcagccatttggtactgaacccggggatccgttccagagcagaaagtccttcacagacccttttagtggca
aggacccgtttgccacatcacagtcgggaaagcactctaagggaacatctgctgggtttgcaaactttggctcttttgggaacgaggagcaacagcttgc
ttgggcaaaacgcgagagccagcgggctgagaaggaaagactagaccgtttaaaacgtcaggagcaggaagacctggagctagcaattgccctcagcaaa
gcagagatgccgagttcctgatagtcgcactctcctggtcttccccatcctgtgcccgcaggattatatgccaacgctattgcaaccgacaccaactctg
cattcaggacagaggtggacaggaagccgaatgacttcatcctaaactagcggtctttatttctgaactactagccagtcacggggaagctgtggccatg
acatcaccataaaatgaccccaatggaactgcttgatgggaatattcacctgcacatatattctaagcaaaggaactgctatccagtcatctcttgtttt
attttatttttctatatcagggaaatattttttttttaatcatccttgctatcccctttacatttcatctgatgccctgtttgaattttacaccaagtaa
gcatttgcttttatttgtacgataagctgttatggcctgcatggaatgactgggctgtttggagtgaatgtaagatgttcagttgatccaaaaccctatg
cctacattaaaaacaatttttacaggtaccatcccgcagaaaaaaaaaaaaaaaa
5' EST: CX911683 [+]ccgcggacggcaggaaagatggcggcgctcatccctctcacacagctttccggtggcaatccattttatgaaacatattacaagcaggtggatccatcat
acattggcagagtaggaccagcagaagctgctctcttcctcaaaaaatctgctctctcagacattatccttgggaagatatgggacctggctgatccaga
gggaaaaggttacttggataaacatggattttatgtagcactgaggctggtagcttgcgctcagagcggacatgaagtcagtctatcaagtttaaatgtt
ccagtaccagcacccaaatttcatgacactagcagtcctctgttaatcactccaccccctgttgagggacattggggtgttcggatagaagagaaatcca
aatttgatggtatttttgacagtctggtgccagtgaatggcttgctttctggagacaaggtgaaacctgtacttatgaactccaagctacctctggatgt
cttaggaagggtgtgggagctcagtgatattgacaaggatggacacttggacagagatgaatttgctgtagcaatgcatcttgtatacagggccttggag
aaggaaccagtcccttcagtgttacctccatctctcatcccgccgaacaagaggaagaagactccaacattcccaggggcagtttcagttcttccctcca
gcccaccacctaaagatagtctacggtccaccccatctcatggcagtatgagcagtttaaacagtgctggcagcctttcacctaagcattcctccaaacc
agcccagcctgctgtaaac
3' EST: CX911682 [+]gcgggatggtacctgtaaaaattgtttttaatgtaggcatagggttttggatcaactgaacatcttacattcactccaaacagcccagtcattccatgca
ggccataacagcttatcgtacaaataaaagcaaatgcttacttggtgtaaaattcaaacagggcatcagatgaaatgtaaaggggatagcaaggatgatt
aaaaaaaaaatatttccctgatatagaaaaataaaataaaacaagagatgactggatagcagttcctttgcttagaatatatgtgcaggtgaatattccc
atcaagcagttccattggggtcattttatggtgatgtcatggccacagcttccccgtgactggctagtagttcagaaataaagaccgctagtttaggatg
aagtcattcggcttcctgtccacctctgtcctgaatgcagagttggtgtcggttgcaatagcgttggcatataatcctgcgggcacaggatggggaagac
caggagagtgcgactatcaggaactcggcatctctgctttgctgagggcaattgctagctccaggtcttcctgctcctgacgttttaaacggtctagtct
ttccttctcagcccgctggctctcgcgttttgcccaagcaagctgttgctcctcgttcccaaaagagccaaagtttgcaaacccagcagatgttccctta
gagtgctttcccgactgtgatgtggcaaacgggtccttgccactaaaagggtctgtgaaggactttctgctctggaacggatccccgggttcagtaccaa
tggctgaaatggatcatgagacttctg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: