XB-CLONE-3253837
Clone Name: IMAGE:7689809
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Gene:
prkca
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC125756 [+]
GGAGGCGGCTCCCCAGCCCGGAGCTGTCAGTGGGGCAGTGGTTGCCCGTTTGGATTGGGACTTTCCGCGGTACCGGAGCCCCCAGCACCATGGCCGATGT
CTTCCAGCACAACGACAGCACCTCGTCCTCCTCCTCCTCCCAGGAGGTAGCGCAGCGCTTTGCCCGCAAGGGGGCCCTCAGGCAGAAGAACGTCCATGAG
GTGAAGAACCACAAATTCATCGCTCGCTTCTTCAAGCAGCCAACGTTCTGCAGCCACTGCACCGACTTTATATGGGGCTTCGGGAAGCAAGGGTTCCAGT
GCCAAGTTTGCTGCTTCGTGGTTCACAAGCGCTGCCATGAGTTCGTCACGTTCACCTGCCCCGGGGCAGATAAAGGCCCAGACACAGATGACCCAAGAAG
TAAACACAAGTTTAAAATCCACACGTACGGGAGCCCCACCTTCTGCGACCACTGCGGCTCCCTCCTCTATGGGCTCATCCACCAGGGAATGAAGTGCGAA
ACCTGCGACATGAATGTACATAAAGTCTGTGTCATCAATGTGCCCAGCTTGTGCGGAATGGACCACACGGAGAAGAGGGGGCGGATTTACTTGAAAGCAG
AAGTGAAAGGAGACGTGTTAAATGTCATAGTCCGTGACGCCAAAAACCTGATCCCTATGGATCCCAACGGCCTATCAGACCCCTACGTCAAGTTGAAACT
GATCCCCGACCCCAAGAACGAAAGCAAGAAGAAAACCAAAACCTTCCGCTCCACCCTCAACCCCCAGTGGAACGAGTGCTTCACCTTCAAGCTGAAGCCG
ACAGACAAAGACAGGAGACTCTCGGTAGAGATCTGGGACTGGGACCGGACCACCAGGAACGACTTCATGGGATCCTTGTCCTTCGGGGTCTCCGAGCTGA
TGAAAATGCCGGCGTGTGGTTGGTACAAGCTGCTGAACCAGGAGGAGGGCGAGTATTACAACGTGCCGATCCCCGAGGAGGAGGATGATGGGAACATTGA
GCTGCGGCAGAAATTTGAGAAAGCCAAACTGGGCCCCGCCGGGAACAAAGTCATCAGCCCCACGGACGAGCGGCGCCCCTACGTCCCCTCCAACAACATC
GACAGCGTCCGACTCACCGACTTCTCCTTCCTCATGGTGCTGGGCAAGGGCAGCTTCGGCAAGGTCATGTTGGCGGAAAGGAAGGGGGCGGACGAGCTCT
TTGCCATCAAGATCCTGAAAAAGGACGTGGTGATCCAGGACGATGACGTGGAATGTACCATGGTGGAGAAGCGGGTACTGGCGCTGCAGGGCAAGCCGCC
CTTCCTCACCCACCTGCACTCCTGCTTCCAGACTGTGGATCGCCTGTACTTCGTCATGGAATACGTGAACGGGGGGGACCTGATGTACCACATCCAGCAA
GTGGGCAAATTCAAGGAGCCCCAAGCCGTGTTTTACGCAGCAGAGATCTCCGTTGGGCTGTTCTTTCTGCACAAGAAGGGGATTGTGTACAGAGACTTGA
AGTTGGACAACGTCATGTTGGACTCCGAGGGACACATTAAAATTGCCGATTTCGGGATGTGCAAGGAGCACATGGTAGACGGGGTAACGACCCGGACCTT
CTGCGGCACCCCTGATTATATTGCCCCGGAGATCATTGCTTACCAGCCCTACGGGAGGTCGGTGGATTGGTGGGCGTATGGAGTCTTGTTGTACGAGATG
TTGGCCGGGCAGCCGCCGTTTGATGGCGAGGATGAGGATGAGCTCTTCCAGTCTATTATGGAGCACAACGTCTCCTACCCGAAATCTATGTCCAAAGAAG
CTGTGTCCATTTGCAAAGGGTTAATGACGAAGCACCCAGTGAAGAGATTGGGGTGCGGGCCGGAGGGGGAGCGGGATATTAGGGAACACACATTCTTCCG
CCGGATCGACTGGGAGAAGCTGCAGAACAGAGAGATCCAACCGCCATTCAAGCCGAAAGTGTGCGGAAAGGGGGCAGAAAACTTCGACAAGTTCTTCACC
AGGGGGCAACCGGTACTGACGCCCCCCGACCAGTTGGTCATCGCCAACATTGACCAGGCCGACTTCGAGGGCTTCTCCTACATCAACCCCCAGTTTGTGC
ACCCCGGCCTGCAGCTTGTGGCGTGATTGGCCAGAGACGCAAACGCAAGAAGATCCATTCCAACCCTACAATTTCAGGGCCTGGCCCCCCCCCCGGCGCA
TCCCGGGACCCTGACAATTCTAGGGTTGGGCGCCATGTTGCTGAGGGACTGTAGCGGCAGTGCCACAGCCAGAAGTATTGGGCGCGGCCGTGTCTCTCCA
TTGGCCACTCGGGGGTCACATGGGGTCTTGGGTGCATTGTGATGTCACCCCATAACGCCATCCGTTGCCATGAGAGACGGTCCTAGCGACCCATACCCTT
GGTGTTGCCGGGGGTTGCGCTCTTACTAAATCCGTAACGGTACTAAATCCCAAGGGTGCAACTCCCCGCCCCCCCCCCCCCCCCCCAGTGCCATATGCCT
TACAGTAACGGCTAACGTGGCTAATCTGCCCCACAGAGGGGCAGGAGGGGGAGATCATATCCCATTGGCCCAATCCATGGAGTATTACCCACAGCAGTTT
TATACGCCAAATATCTGCTCTCTCTTTCCTTTGGCTCCTAACGAGCACTTAATGAGGTCTCCTTGTATCTTTAGGGTTAATAAATCATGGCCACACAGGT
TGGGGGCGTGGCTTTTATTCTGATTTAATGCCATGGGGGTCATATCTGTCCCCCCCCAGGCACCCCCAGTGTCCTCCCATTCTTATATTGTGTCATTAAT
CTCATTCATATTAAGCCCCGCCCCTTTGCCCCGTATGGGGGCAGATTTGTGTTGCGCTGCATGCGAGGGGCCGGTGCATGGGGATTGGCCAGCGGCTCGT
GTTGCATGTCGGCCTCCCGGGATCCCATTCGCCACAAATACGGGAATCTTTTCTTTCCTCGTCAGTCGCCAATAATCACTCGCTCGTTATGGTTTGTGCC
AAAATGTCTGTAATTAACATTTCCCAATCACTTTCTCATGGTGTCGGCCGTACGATTGGCCGCCATGTTTGCTCAAAGCATCCGCCAATGATTCGCCAAT
GATCCCAGTCCCCGGGGCGGGGGGGAAACTGTTATGGTTAGAAATGCACAATTCTGTAAATACGATCCTATTGGTGAATAAATCATTTCCTGTCTCAAAA
AAAAAAAAAAAA
5' EST: CX913448 [+]gaggcggctccccagcccggagctgtcagtggggcagtggttgcccgtttggattgggactttccgcggtaccggagcccccagcaccatggccgatgtc
ttccagcacaacgacagcacctcgtcctcctcctcctcccaggaggtagcgcagcgctttgcccgcaagggggccctcaggcagaagaacgtccatgagg
tgaagaaccacaaattcatcgctcgcttcttcaagcagccaacgttctgcagccactgcaccgactttatatggggcttcgggaagcaagggttccagtg
ccaagtttgctgcttcgtggttcacaagcgctgccatgagttcgtcacgttcacctgccccggggcagataaaggcccagacacagatgacccaagaagt
aaacacaagtttaaaatccacacgtacgggagccccaccttctgcgaccactgcggctccctcctctatgggctcatccaccagggaatgaagtgcgaaa
cctgcgacatgaatgtacataaagtctgtgtcatcaatgtgcccagcttgtgcggaatggaccacacggagaagagggggcggatttacttgaaagcaga
agtgaaaggagacgtgttaaatgtcatagtccgtgacgccaaaaacctgatccctatggatcccaacggcctatcagacccctacgtcaagttgaaactg
atccccgaccccaagaacgaaagcaagaagaaaaccaaaaccttccgctccaccctcaacccccagtggaacgagtgcttcacctttcagctgaagccga
cagacaaagacaggagactctcggtagagatctgggac
3' EST: CX913447 [+]ttttttttttttttttgagacaggaaatgatttattcaccaataggatcgtatttacagaattgtgcatttctaaccataacagtttcccccccgccccg
gggactgggatcattggcgaatcattggcggatgctttgagcaaacatggcggccaatcgtacggccgacaccatgagaaagtgattgggaaatgttaat
tacagacattttggcacaaaccataacgagcgagtgattattggcgactgacgaggaaagaaaagattcccgtatttgtggcgaatgggatcccgggagg
ccgacatgcaacacgagccgctggccaatccccatgcaccggcccctcgcatgcagcgcaacacaaatctgcccccatacggggcaaaggggcggggctt
aatatgaatgagattaatgacacaatataagaatgggaggacactgggggtgcctggggggggacagatatgacccccatggcattaaatcagaataaaa
gccacgcccccaacctgtgtggccatgatttattaaccctaaagatacaaggagacctcattaagtgctcgttaggagccaaaggaaagagagagcagat
atttggcgtataaaactgctgtgggtaatactccatggattgggccaatgggatatgatctccccctcctgcccctctgtggggcagattagccacgtta
gccgttactgtaaggcatatggcactgggggggggggggggggcccggaa
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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