XB-CLONE-3253721
Clone Name: IMAGE:7689548
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Gene:
brpf1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC154088 [+]
GATGGAAGTTGTAACGCTGTGTGGGATTCGCTGTCAGGCAGGAACCAAGGAGCTGGACAATAATGGCGCTGGGGGTTGTTTAAGCGGCGATCCGGGACTG
CGGGTTTTGCTGAGAGAATTCGGACTGTCCCGCTAAAAGCGGGACTGTTGTGCGGTAGAGTCCAGATGTAACATGATGGGTGTTGACTTTGATGTGAAGA
CATTTTGCCACAACTTGAGGGCCACCAAGCCTCCCTATGAATGTCCGGTGGAGACCTGCCGAAAGATCTACAAGAGCTACAGTGGGATCGAGTACCACCT
GTATCACTACGATCATGACAACCCGCCTCTGCAGCAGCAGACTCCTATCCGCAAATCAAAAAAGAAAGGCCGGCACCCCCGATCCGCCAATAAGCAGTCA
CCGACGAACTCGGAAGTGTCTCAGTCTCCGACCCGGGAGGTGATGACTTACGCCCAGGCCCAGAGACTGGTGGAGGTGGAATTGCACGGGCGCGTGCATC
GTATAAGTATATTTGACAACTTGGATGTTGTTTCTGAGGATGATGACCCGCCCGAAGAAACCCCAGAAAATGGAAGTAATAAGGAAAATAATGACACCCC
CGCGACCACTCCTAAGTCGGGAAAGCACAAAAACAAGGATAAGAGAAAAGACTCCAACCATCATCATCACCACAGTGCCACCGCAGGCGCCGCCACCAAA
CTCCCAGAGGTTGTGTACCGAGAGCTCGAACTGGACTCGCCAGATGCTCCTCCGCGGCCTTCTTCCTATTACAGACTCAGTAAGATCACAAACCGCTTGA
CCATCCAGAGGAAGAGTCAGTTTATGCAAAGGTTACACAGCTACTGGACCTTAAAGAGACAGTCTCGGAATGGTGTCCCGCTTCTACGTCGCCTGCAGAC
GCACCTACAGTCTCAAAGGAACTGCGACCAGGAGATGAAGTGGCGGATACCAATTTCTGATGACACGCCAGGTCACCTTGGTACCATCGCTATTTCTAAC
GAGGAGCCCGGAGACCAAGCTAACAAGAATGACCCTACGGATAAGAACTGGGTGCTAAAGGAACAATTAAAGTCATGGCAGAGACTTCGGCACGACTTGG
AGAGAGCTCGCCTTCTGGTAGAATTAATACGAAAGCGCGAGAAGCTCAAGAGAGAGACGATTAAAGTCCAGCAAATGGCACTGGAAATGCAGCTGACTCC
ATTTCTTATCCTACTGAGAAAAACCCTGGAGCTGCTGCAGGAGAAAGATACAAGTAATATATTCACTGAGCCAGTCCCTCTGTCTGAGGTAACAGAAATC
TACGAAGTGCCAGACTACCTAGAACATGTTAAGAAGCCAATGGACTTTCAGACCATGAAGCGTAACTTAGAGGCTTTCCGTTACCAGAATTTCGACCAGT
TTGAGGAAGACTTCAACTTAATTGTGAACAATTGCATAAAGTACAACGCTAAAGACACTATTTTTTACCGAGCCGCATCCCGCCTACGAGAACAGGGAGC
AGCCCTGCTGCGGCAGGCCCGCAAGCAGGCGGAAAAGATTGGCATTGATTTCGAGACGGGTATGCATATACACCCAGTCATCACAGGAAATGAGATTGGG
CGCCATGGCAGAGAGAATGACTTTGAGCGTGCTCATCTGTCTGACAGCCGCAAGCAACTCCCTTTAGAGGAGCAGCTGAAAGTCCAGTTGGCGAGACTGG
ACGAGGTGAATGCAAGTAAGCAAAGCATAGGACGAGCGCGCCGGGCCAAGATGATCCGCAAGGAGATCACGTCGATCCGTAGGAAGATGGTTTACCAACA
AGAATCCAGAAGGGACGGTGGCTCTGAGCGGCACAGCTCAGCCGGGAGAGGAGCCCTGCCTTTGCACGGCCCTTCAGAGAAGGATGGACAGACGGACAGT
GCTGCAGAAGAGAGCAGCAGCCAAGAGACCGGGAAAGGACTTGGCCCAAACACTTCCTCCACCAATGCTCATGAAGTTGGTAGAAGGACTTCTGTCCTTT
TCTCTAAGAAGAACCCCAAAACTGCAGGACCACCTAAGAGACCAGGAAGGCCACCTAAGAATAGAGACAATCAGCTCTCATCGGGACAGATCAGCAGCCC
CATTGGTCCCCCACAGCTGTCTGTCACAGGGGGATCTCAAAGGCGAAAGAGAGTCCGAAGCCCTCGGCCAACTTCAAGCTCTGATAGCGACAGTGACAGT
GAAAAGTCAGCAGAGGAACCCTCTTTAGATTTGCCTACAAATGGCTTCAGCAGCAGCACCCAGCAAGTCAAAAAGAGTTTTTTGGTTTACCGGACCGACT
GCAATCTTCCACGAAGCAGCTCAGACTCTGAGTCCAGCACCACCAGCAGCAGCAGTGCTGCTTCCGACAGGACCAGCACAACTCCTTCCAAGCAGGGTCG
GGGGAAGCCATCTTTCTCTAGGGATAATTTTGCTGACTACAGCAGTGAGGATACATCTGGAACCGAGAATGAGTCTTACTCCGTTGGTGGGAGAGTCGGG
CACGGCTTGGTACGCAAAGGACTGGAAAGCCGAGGGGCAGGGTGGCTGTCTGAAGATGAAGATTCCCCTCTAGAAGCTTTAGACTTGGTGTGGGCCAAGT
GTCGGGGCTATCCCTCCTATCCAGCTCTGATCATTGACCCAAAAATGCCACGAGAAGGAGTGTTTCACCATGGCGTTCCGATTCCTGTGCCACCCGTGGA
TGTTCTCAAATTAGGAGAGCAGATGGCCCAAGAGGCACAAGAGCATCTCTACCTCGTCCTGTTTTTTGACAACAAGAGAACCTGGCAGTGGCTTCCTCGG
ACAAAGTTAGTCCCTCTCGGCATGAACCGGGAATTGGATAAGGAGAAGATGCTGGAAGGAAGAAAATCAAACATCCGTAAATCTGTGCAGATCGCCTATG
ACCGTGCCATGCAGCACCGTAACAAAGTACAGGGCGATCCAAGCAGTGAATCCAGTGAGAGCGACTAGCGTTGCCATCTCCACAGCAGGAAACGACGGAA
GCTTCCTGCACGCCACTGTCAATCAAAAAAAAAAAAAAA
5' EST: CX913629 [+]tggaagttgtaacgctgtgtgggattcgctgtcaggcaggaaccaaggagctggacaataatggcgctgggggttgtttaagcggcgatccgggactgcg
ggttttgctgagagaattcggactgtcccgctaaaagcgggactgttgtgcggtagagtccagatgtaacatgatgggtgttgactttgatgtgaagaca
ttttgccacaacttgagggccaccaagcctccctatgaatgtccggtggagacctgccgaaagatctacaagagctacagtgggatcgagtaccacctgt
atcactacgatcatgacaacccgcctctgcagcagcagactcctatccgcaaatcaaaaaagaaaggccggcacccccgatccgccaataagcagtcacc
gacgaactcggaagtgtctcagtctccgacccgggaggtgatgacttacgcccaggcccagagactggtggaggtggaattgcacgggcgcgtgcatcgt
ataagtatatttgacaacttggatgttgtttctgaggatgatgacccgcccgaagaaaccccagaaaatggaagtaataaggaaaataatgacacccccg
cgaccactcctaagtcgggaaagcacaaaaacaaggataagagaaaagactccaaccatcatcatcaccacagtgccaccgcaggcgccgccaccaaact
cccagaggttgtgtaccgagagctcgaactggactcgccagatgctcctccgcggccttcttcctattacagactcagtaagatcacaaaccgcttgacc
atccagaggaagagtcagtttatgcaaaggttacacagctactggaccttaagagacagtctcggaatggtgtcccgcttctacgtcgcctgcagacgca
c
3' EST: CX913628 [+]gacagtggcgtgcaggaagcttccgtcgtttcctgctgtggagatggcaacgctagtcgctctcactggattcactgcttggatcgccctgtactttgtt
acggtgctgcatggcacggtcataggcgatctgcacagatttacggatgtttgattttcttccttccagcatcttctccttatccaattcccggttcatg
ccgagagggactaactttgtccgaggaagccactgccaggttctcttgttgtcaaaaaacaggacgaggtagagatgctcttgtgcctcttgggccatct
gctctcctaatttgagaacatccacgggtggcacaggaatcggaacgccatggtgaaacactccttctcgtggcatttttgggtcaatgatcagagctgg
ataggagggatagccccgacacttggcccacaccaagtctaaagcttctagaggggaatcttcatcttcagacagccaccctgcccctcggctttccagt
cctttgcgtaccaagccgtgcccgactctcccaccaacggagtaagactcattctcggttccagatgtatcctcactgctgtagtcagcaaaattatccc
tagagaaagatggcttcccccgaccctgcttggaaggagttgtgctggtcctgtcggaagcagcactgctgctgctggtggtgctggactcagagtctga
gctgcttcgtggaagattgcagtcggtccggtaaaccaaaaaactctttttgacttgctgggtgctgctgctgaagccatttgtaggcaaatctaaagag
ggttcctctgctgacttttcactgtcactgtcgctatc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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