XB-CLONE-3253597
Clone Name: IMAGE:7689871
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Gene:
ddx43
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121888 [+]
GGATTCTGGTAAGGCGCAGTAGCAGCTACGAAGCATGTCGGACTGGGAGGAGGAGAGCGATAGTAGCTCTAACCAGGCTGCAGCTCGGTACAACACCCCT
TGGGTGCCTCCATCTAAGCAGTATTCGAGGGAGGAGCCGCGCACAAAAACGCAGTCAGAATTCTGTGGGTGGCGTGAAGACAACGACGATGGTGGTGGGA
GAAGCCGGTCTGAGCAGGGATCCTGGAGGGGTTTCTCACGGCCCCGAGGATCAGGACCCCCTGGAGCAAGTACACGAGAGTTCAGGAGGAGGGAGGGCGC
TCGGGATCAGAGTTCCCCCCTGTGCTTCCATCTAGACAGCTCGGCCATTGGGCAAGTGATAGGGCGTGGAGGATCTAAAATAAGGGAGCTTGAAGAACTG
TCTGGCTGCAGGATAAAGATTGAGAAATTGGATTATGATTCAGAGGTTAAAATCTACGGAAACAGTGAGCAGCAACAGAAAGCCAAAGAGTTAATTGATG
ATATTGTTGAACAATCTGAGAGAGGTCCTGGTTTCAAGGGTTTTCGGTCTTCTGGTTCTGCAGATAAGAGTGCTGATGCGAGTTTTCAGGTGAAACCATC
CATTAACTGGGCATCTCTTCGTGAAAATAAATCAAAATATGAAGCCATGAAGTGGGCAGATATGCCTCCAATTGCAAAAGCCTTCTACAAAGAATCACCA
GAGGTTGCATCAATGTCTCGTGATGAAGTTGAAAAGTGGAGAAAAGAAAACAACAATATTATTTGTGATGACTTAAAAGAAGAGAAAAGATCTATTCCAA
ATCCCGTTCCTCGTTTTGAAGATGCATTTCACCCATTCCCAGAAGTCATGTCTGCATTAGAAAAATCTGGATTTGAAAGACCCACACCAATACAGTCACA
GGCATGGCCAGTTATACTTCAGGGAATTGATCTCATTGGAATTGCCCAGACTGGCACTGGAAAAACATTGGCATATTTACTTCCAGGATTCATTCATTTG
GATTTACAACCAATTCCCAGAGAGCAACAAGATGGACCTGGAATGTTGGTTCTTGCTCCAACTCGTGAATTGGCTCTTCAAGTTAAGGCTGAATGCTCAA
AATACAAATATAAAGGATTTGAAAGCATTTGCATATACGGTGGAGGAGATCGTAATAACCAAATAAATAAGGTTACAAAAGGTGTGGATATTGTCATTGC
TACTCCTGGTAGACTAAATGATTTGCAGATGAATAATTTTGTAAATCTAAAAAGCATAACATATTTGGTTTTGGATGAAGCAGACCGAATGTTGGATATG
GGATTTGAACCGCAAATTATGAAGATATTAATTGATATTCGACCTGATAGACATACTATAATGACAAGTGCCACATGGCCAGATGGAGTACGCCGCCTTG
CTAAATCATATTTAAAAGATCCTATGATGGTTTATGTTGGAACACTCGACTTGGCTGCTGTAAACACAGTAACACAGCACGTATTGGTTATACCAGAAGA
GGAAAAAAGAGCATTTGTATTGCACTTCATTGACTCTTTAAAACCTCAAGATAAAGTCATTGTATTTGTTGGGAAGAAGTTGGTTGCAGATGACTTATCA
AGTGACTTCAGCTTGCAAGGAATTCCGGTTCAGTCTCTGCATGGCAACAGAGAGCAATGTGATCGAGAGCAAGCACTAGATGACTTCAAAAAGGGGAAAG
TCCGAATACTAGTAGCTACAGATCTAGCATCTCGAGGACTTGATGTTCATGATGTTACACATGTTTTAAATTTTGATTTCCCTCGAAACATAGAGGAGTA
TGTTCACAGAGTTGGACGCACAGGAAGAGCAGGTCGCACAGGGGAATCAATAACTCTGGTAACCAGGAAAGACTGGAAGGTTGCAGGTGAATTAATCAGT
ATCTTGGAGCGAGCTAATCAAGAAGTTCCAGGGGATCTTTTTGATATGGCTGAAAGATACCAGCAATTTAAGATAAAGAAGGATAGTGAAAGAGACCTTA
TACCCCGCAAAGGACGCTGGAGAGAAAGCTTCTGATTTCAGTTGTTGCATCATGACATGACTGTTTGAATGTTAAACCAATACTTGCTATCACCAGCCAT
TCCTATATCATTGGAGTATTCTTGGGAAGAAGTTTTACGTTTTATGTTAGCAAATTTACTACATGTATATTTGTTTCTTATGTTCCAAAACAATGCTATT
GCCATTCAGCATGAATCCATTTGAACAATTCATTTAGTTATTTCTTTTTGGATTTCAAACTGCAGTACAGAACAGATATCAACAGATTTCACAATTGCTC
AGTGTAAACCATAATAACTTAGGTAGGGTCAAATCTTTTTTGTCACACTTGTAAATATGTTTTGAAGCCTTTGGGGCTGTGGCACGCACCCTGTTTTATT
GCTGCTACTTGTGGCAATGATAACATCTGCTACTGTTCCCAGACTGCTTTCCATTTGACTTTGGGTTGGCTGGCGCTCTATGCTAACTTTTGGAAACCAT
CTGTTCTTATTTATGTGAATAACAGTGGTGTTCTGATTGCTGCCATAAGTAGTAAGTATAAGTGATCAGATTGCAGAGTTTGTCATCAGTTTCGGTGCTT
TCAAAATAATCCTATATATTAATGAGGTGTAGGATGTGTCATTGCCATTTAACTGATAAAACTTGCTGCAGTTGATGGTGGTAAAAATGCCAGATGCATT
GTTTTTGAGTATCAAAAACTTAGACATGAAGATTTATTCTTAGTAAAGCCTTACATAAAAAGTTGTTAGCCCCACTTGGTTTCACTTCAACGCACCGTTA
CAATAGTGCTGAAGTTTCCAAGTGAAATTGACTGCAGTTTAATGTGGTATCACTCAGCTCTTATTTGCAATATTGTTTCTTTCTGTAATTGACATCCTGC
TGTGAAGAGTAATGTTACAGTGATTTTATCTGTAAGAGAGTCAGAATTGTTCTTGAACGTTGCTTATTAAAAGCTGTGTATCAGAAAAAAAAAAAAAAA
5' EST: CX913894 [+]gattctggtaaggcgcagtagcagctacgaagcatgtcggactgggaggaggagagcgatagtagctctaaccaggctgcagctcggtacaacacccctt
gggtgcctccatctaagcagtattcgagggaggagccgcgcacaaaaacgcagtcagaattctgtgggtggcgtgaagacaacgacgatggtggtgggag
aagccggtctgagcagggatcctggaggggtttctcacggccccgaggatcaggaccccctggagcaagtacacgagagttcaggaggagggagggcgct
cgggatcagagttcccccctgtgcttccatctagacagctcggccattgggcaagtgatagggcgtggaggatctaaaataagggagcttgaagaactgt
ctggctgcaggataaagattgagaaattggattatgattcagaggttaaaatctacggaaacagtgagcagcaacagaaagccaaagagttaattgatga
tattgttgaacaatctgagagaggtcctggtttcaagggttttcggtcttctggttctgcagataagagtgctgatgcgagttttcaggtgaaaccatcc
attaactgggcatctcttcgtgaaaataaatcaaaatatgaagccatgaagtgggcagatatgcctccaattgcaaaagccttctacaaagaatcaccag
aggttgcatcaatgtctcgtgatgaagttgaaaagtggagaaaagaaaacaacaatattatttgtgatgacttaaaagaagagaaaagatctattccaaa
tcccgttcctcgttttgaagatgcatttcacccattcccagaagtcatgtctgcattagaaaaatctggatttgaaaga
3' EST: CX913893 [+]tttttttttttttttctgatacacagccttttaataagcaacgttcaagaacaattctgactctcttacagataaaatcactgtaacattactcttcaca
gcaggatgtcaattacagaaagaaacaatattgcaaataagagctgagtgataccacattaaactgcagtcaatttcacttggaaacttcagcactattg
taacggtgcgttgaagtgaaaccaagtggggctaacaactttttatgtaaggctttactaagaataaatcttcatgtctaagtttttgatactcaaaaac
aatgcatctggcatttttaccaccatcaactgcagcaagttttatcagttaaatggcaatgacacatcctacacctcattaatatataggattattttga
aagcaccgaaactgatgacaaactctgcaatctgatcacttatacttactacttatggcagcaatcagaacaccactgttattcacataaataagaacag
atggtttccaaaagttagcatagagcgccagccaacccaaagtcaaatggaaagcagtctgggaacagtagcagatgttatcattgccacaagtagcagc
aataaaacagggtgcgtgccacagccccaaaggcttcaaaacatatttacaagtgtgacaaaaaagatttgaccctacctaagttattatggtttacact
gagcaattgtgaaatctgttgatatctgttctgtactgcagtttgaaatccaaaaagaaataactaaatgaattgttcaaatggattcatgctgaatggc
aatagcattgttttggaacataagaaacaaatatacatgtagtaaatttgctaacataaaacgtaa
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: