XB-CLONE-3253527
Clone Name: IMAGE:7690225
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Gene:
dop1a
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161397 [+]
atcatgggccaacaaaaccccacagagtttaaccttaacttggaatcacaaagcagaaaaagaagccacagcagtatacagttcaattttataggaaaag
ctactgaaaaagacagttctgctaaagatgtcagcaaacagggtgcaaagcccaagttaaaaatgtctaggaggaaagatgaagataagaaaaaaacaca
gaatgaaaagctaaaacaggcaaatgttttttttagtgatgggttggatttagagaactggtacagctgtggagaaggagaaatttcggaaattgaaagt
gatgtaggttctccaggagtacgtaaatctccacattttaacatccatcctctttatcagcatgtgctgctctaccttcagctttatgactcctcaagga
cattatatgcacttactgccattaaagccatattaaagacaagtcccacagtttttgtaaaagctatttcaaccactaatgttagcaatgcttatacccc
gcaattgtcattgcttcaaaatcttttggcccgccatcggatatctgtaatgggaaaagatttttatagcaatattccactggactcaaatcacaatttt
cgcagttcaatgtatatagaaatccttatatctttatgtctgtactttatgagaagccactatcctactcacgtaaaggtaacgcagcaagatctgatgg
gtaatcgcaacatgcaaatgatgagcatagaaattctgactcttcttttcacagagcttgctaaagttatagaaagctctgcaaagggtttcgccagttt
catatctgatatgttatcaaagtgcaaggttcagaaagtaatacttcattgcttgttgtcatcgatttttagcgctcagaagtggcacagtgaaagaatg
gctgagaagaacataatggcaatagaagagggattctctgaggacagcctaattaatttctcagaagatgagtttgacaatggcagcacattgcagtctc
agcttttaaaggttcttcagagtctgatcattctggagcaccgtgtgctgacagttccagaagataacgaagcaggctttgattttgtcattactgactt
ggaaaacattaatccccagcagccaatgacttcacttcagtacttgcactctcaaccgatcacatgccaggggatgttcctttgtgctgtgatcagggct
ttacatcagcattacacgtgcaaaatgcaccctcagtggatttgccttatgacatccaccttgccttatatggggaaggttctgcaaaaagttgtagttt
cagtgactttacagctttgcagaaatctggataacttaattcagcagtacaagtatgaaactggaataacagactcaaggccactttggatggcatcctt
tattcctccagacatgattctaactgtcttagaaggaattacaacaataattcactattgcttgcttgatccatctgctcagtatcaccagcttatggtg
agtgtggatcagaagcatttacttgaggctcgcagtggaatgctgtccatccttcatctgattatgtcctcagttaccttgctgtggagcgtactgcatc
aagctgactcttcagagaaaaacttcactgctgcttctgcaactgttaccacagtcaatcttggctctacaaagaatttacggcagcagattctggagct
gttgggtccaatttctatgaatcatggggtatattttatggctgccattgcttttgtttggaatgaaagaagacagaacaaaaattctactcgcacaaag
gttataccatctgctagtgacgagcagcttttgttggtggaactggttcgctctattagtgtgatgaggaccgaaacagtgatacagactgtgaaagaag
tattaaagcagccaccggccattgctaaagacaagaagcatctctcacttgaggtctgcatgctacagttcttttatgcctatgttcaaagaattccagt
ctccaatcttgtcgatagttgggcttcttttcttgtgcttctgaaggattctattcagcttaatattccagcaccaggacagtttcttatacttggtgtt
ctaaacgagtttatcatgaaaaacccaaccctggagaacaagaaagaccagagggatttacaggatgtaactcacaaaatagtagatgccattggagcaa
ttgctggttcgtccttggagcagactacatggctaagaagaaatctggaagtcaagccgtcccccaggattactgtagatgggaccaatctagaatctga
tgttgaagatattatgctatccccagtcatggaaatatcaaacattactccatctgtttacagtgtgcatgccctgacattactcgctgaggttcttgca
cacttgttggatatggttttctacagtgatgagaaagagagagttatacctttgcttgttaatattatgcattatgtggttccatacttacgcaatcaca
gtgcacataatgcttccagctacagagcttgtgtgcagcttctgagcagtctgagtggctatcagtatacaaggagaacatggaagaaggaagcatttga
cctttttatggatcccaacttcttccagatggatggatcctgtgttagtcactggagatctattatggaccatctcatgacacatgataaaactacgttt
cgggatttaatgacccgcgttgctgtggcacagagtagttcccttggtctgtttactaaccgtgatgcagagttggagcagcgagccatgcttctcaaga
gactggcatttgccatcttcagcagtgagatagaccagtaccagaagtttctaccagacatacaagaaagattagtggagagcctgaggctgccacaggt
tccaactctgcattcccaagtattcctgttctttcgggttttgcttttaaggatgtctccccagcacctcacttctctgtggccaactatgatcactgaa
ctggtacaagtatttctgcttatggaacaggaactcactgctgatgaagatatcacaaggatatctgggccatcagttgctggtttagaaacaacttaca
ctggaggaaatggcttctctacatcctacaacagccagcggtggctaaacctgtatttgtctgcgtgcaaatttctagatttggccttagccttgccctc
tgaaaaccttcctcagtttcagatgtacagatgggcatttatcccggaagcatcagatgatgcaggcttggaggtgcgcagacaaggcacacatcaaagg
gaattcaaaccatacgttgtgcgccttgcaaagctgctgaggaaaagagcaaagaaaaacccagaggaggactgctcagggagaacactagactggaacc
caggaaacctcctgctaacaatctacagtatccgcaccatcgagcagctgctgccttttttcaacgtactaagccaagtcttcaatagcaaaatcacaag
cagatgcgttggagaatcagggagcccgatactttaccaaagctcttttcccaacaaagacattaaaatggaaaaccagaaactgttctgcaaaataaga
caaaagattgaggagatgcttgagagagattttcttgaagggggtttgaagagctgatctaaagccactagtcatgatcatcacatttgagttgtacagc
atttggattttttttaccttgtatgtactgtatgaaacatgcttcttacttctcattccatcatatttaatttaaatatttttatttgtaaataaaatta
actatgttaaaagagcaaaaaaaaaaaaaaa
5' EST: CX914043 [+]atcatgggccaacaaaaccccacagagtttaaccttaacttggaatcacaaagcagaaaaagaagccacagcagtatacagttcaattttataggaaaag
ctactgaaaaagacagttctgctaaagatgtcagcaaacagggtgcaaagcccaagttaaaaatgtctaggaggaaagatgaagataagaaaaaaacaca
gaatgaaaagctaaaacaggcaaatgttttttttagtgatgggttggatttagagaactggtacagctgtggagaaggagaaatttcggaaattgaaagt
gatgtaggttctccaggagtacgtaaatctccacattttaacatccatcctctttatcagcatgtgctgctctaccttcagctttatgactcctcaagga
cattatatgcacttactgccattaaagccatattaaagacaagtcccacagtttttgtaaaagctatttcaaccactaatgttagcaatgcttatacccc
gcaattgtcattgcttcaaaatcttttggcccgccatcggatatctgtaatgggaaaagatttttatagcaatattccactggactcaaatcacaatttt
cgcagttcaatgtatatagaaatccttatatctttatgtctgtactttatgagaagccactatcctactcacgtaaaggtaacgcagcaagatctgatgg
gtaatcgcaacatgcaaatgatgagcatagaaattctgactcttcttttcacagagcttgctaaagttatagaaagctctgcanagggtttcgccagttt
catatctgatatgttatcaaagtgcaaggttcagaaagtaatacttcattgcttgttgtcatcgatttttagcgctc
3' EST: CX914042 [+]tttttttttttttttgctcttttaacatagttaattttatttacaaataaaaatatttaaattaaatatgatggaatgagaagtaagaagcatgtttcat
acagtacatacaaggtaaaaaaaatccaaatgctgtacaactcaaatgtgatgatcatgactagtggctttagatcagctcttcaaacccccttcaagaa
aatctctctcaagcatctcctcaatcttttgtcttattttgcagaacagtttctggttttccattttaatgtctttgttgggaaaagagctttggtaaag
tatcgggctccctgattctccaacgcatctgcttgtgattttgctattgaagacttggcttagtacgttgaaaaaaggcagcagctgctcgatggtgcgg
atactgtagattgttagcaggaggtttcctgggttccagtctagtgttctccctgagcagtcctcctctgggtttttctttgctcttttcctcagcagct
ttgcaaggcgcacaacgtatggtttgaattccctttgatgtgtgccttgtctgcgcacctccaagcctgcatcatctgatgcttccgggataaatgccca
tctgtacatctgaaactgaggaaggttttcagagggcaaggctaaggccaaatctagaaatttgcacgcagacaaatacaggtttagccaccgctggctg
ttgtaggatgtagagaagccatttcctccagtgtaagttgtttctaaacccagcactgatggcccagatatccttgtgatatcttcatcagcagtgagtt
ccctgttccatagcagaaatacttgtaccagttcagtgatcatagttggc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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