XB-CLONE-3253317
Clone Name: IMAGE:7690178
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Gene:
morc1
,
colec12
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161396 [+]
gggggcgctagtgcagtttaaagtagttctgcttgtgagagagtttcctgcgtccgcaaaagtgttgggtatcgttatcgccagccagaccttagtaaat
tccacgcagcgctgggcagtccgagtgcaagtgatggctgaacgctacaagtgcttatccagagcgaaccttaacctagcatatatccatgccaactcaa
caacacatgagtttttatttggggcatttgcggaaattattgataattcaagagatgcaaatgcctcaacactttacatatattctgaaccaacaagaga
ttttcaaggacagctgttgtgttttttggatgacgggtgtggcatgactcaccgagaagcctgtgatctgatttactttggtagatcttccaagcgcttc
acaagttctaggtttattgggagatatggtaacggcctgaaatctggttccatgagaattggtaaagactttatactgtttacaatgagagaggattgta
tgacatgtctgcttttttcacaaacattttgtgaaaccgaaggacttgatgagcttattgtaccgattctttgctggtcacaaagcaccaaaaagccttt
aacggaaagctctgatatagccgacatacaaatgtcaattttaaaccgatattctccatttaaaaccgaacaggacttactgaagcaatttgaatatata
tacactggtcacggaactttaattattgtctacaatctaaagctgatgtccaacagcgaaccagagttggatttcttcacagatatctcagatattataa
atgctggaatgagagatagtgatgtctattctgaactgtggtcactgaaggcctatatctcagtattgtatgttgatcctcgaatgaaggtctttgttca
agcaacaagagttcataataagcagctgatatacagtttatatagacccaggatgtatctgtacaaaatgccttctctaagaacatctgttttgcgtgag
ataaaaaatgcagaagtagctgtaaaaaatgctaacctagctgtagctgatatagaacagaagatggctgagcatgtcagtgagaaagagctggaagttt
tacattgtttattaatcaacaacaagaagaaactagatgagaagtgtcagatactaaaagagaagaaaagagaaaaacagttaaaacacgtttatataaa
atatggcctaaacatagaaactagaagtcaagatggaatgtttatttatcataacaaccgcttaatccgaatgcatgagaaagttgggcgccagcttaat
aaagaactaagcactggtgcaggtgttttgggattggtgaacataccttcaggagcactgattccaagccacaacaaacaaaattttattaattcacagg
aatatcataagctattaaaacacatgggctactttcttgaccagtttttgaaggattctgggataggtgagaaaggaacagttcatttctggaatgagtt
tggatacctcagtgggttaaaagactggtttgaagttccatccgatgctgtgcactttatacgaagacgtgcaatagaaatccctaaaatcattcaatgt
gacttgtgcctaaactggagggtgctgccctcttctgcaagcacttttgatcagcctgataactggacttgtgcagaccacccagatcctacaaaaaaca
gatgcacagagccagagcagctccctgctatacctgttggtactttgcccagaaaacctctccagttcactgacaaagaaaaacgcctaagagattccgt
tgaaagatacaagaacaaacgcttggggtctcagaagtgcaatttaactgagcctcatttagctgttccagcattactaagcatatcttccaaaaagagg
aaggatgaaagttcaagaacagatattttatctaaaaggatggctgattctcaaaaatataaagaaagcagccaaaacagagttgttacaaggaaacata
acagagacagatacagactgaatgacagctctataataaccaaaggtccacacccctccagcagactaaatcttcccgtaagtaaaactgaactgagaat
atgcacaactaaagttggcaaaacccttgagaaacctttgtgctcagccagaagtcagacagaaaacgccaaaaattttattttaggggcacctgcacca
tcagcttcatcagtaagtgaaaataatgtaagtaaatgcattgctgaagctggaagaactcttgaggttccttcctgctcaagaaaggagacagaagaaa
aacttaagctaacattaggagaggtaatctcatattttgtaccagactgccagctgccaatacttgagactatatataagggagaccaggcacagttggg
atctctggtaaatgactacttcaaggaatatgaagaaaatgtcctagcagtatatttgcatgctgcaaatgaccattttgaagccaaagctgaattaaat
gagttaatgggggaagcctgtgaagaacagataaaagtcgtggaacaaaaaatttcagcattaagcaaaaaagctgcaaggatctttaaaaaactccata
ttaatgtgccagaggagagtgttttggacacagaaaggcttctacttcaagaccaaagtgaaatttgagagacaaaaccttgtgtaaaagattattttta
ccgtgtggcttctgtaaaatacagattaacgctgtcttctaagattccataagcattgccattctacaagcatggacatgtgtaaggaacatgtgccaag
ctggaagccttctccattgtgtagttactgcttagatacaggatattttatattttttagaattatagatactatttttattcctagatataagactttt
gtaatcttaaaaaaacaaatatataatatttatatctgtgtttttcagatctttccacttgtatacaaatatgtacatttacccttgatcattgcaaact
gcagatgatcatttttgggccttcttccttatgtttaagatctttgtattttcttttttttaagagatattgaaagtgtgatttaaatattgtggcaata
aatgtttcagttgaacagtgaaaaaaaaaaaaaaaa
5' EST: CX914393 [+]cgtgtgcagggaattgtgggatttggaggatgcaggctgagaacagttgacaactgttacattttatttgagtctcaaagtagtcagccaaatcagcagg
agaacaggggaccaggcttagacgactttgctgaagaagaggaggtacagtcgtttggatacaagaggtttggtatacaagaaggaacacaatgtacaaa
gtgtaaaaataactgggcactgaagttttctatcatactgctgtatgttctgtgtgcgctgctaaccatcacagtgtctatactgggatacaaagttgta
gaaagaatggatggggttactgatgacatggaactgtcacacaggggctactcaaagcaactttcagatgtggaagtgaatttgaagaaattagatgatc
aagcgggattaaaatccatctctaccagtagtgagctttcaagcgtcaggtcagacattgccaccatacgccagcaactgcgagaaatcagcgagaaaac
aactcgaaataaagacaccttagaaaagttgcaggaagctggagatttactagatgccaagcaaagccaaatgaaaactgtgctggacaacaactcttta
atgatcaatggtctcaatcacactttactggcttataatacctatgtctccagcttacagcaggatacaaacagcctacagacaagcctgcaaaaccaaa
tgcagttgagtagtgtaatcatcatgaatatcagtaacctaaatgtcacccagattcaacagaggactgccataagtgcactgcagagatctgttgatga
cacgagtcaagctattcaaagaatc
3' EST: CX914392 [+]gaatgtagtttttactgacatctttgtaaaaagaaaactccagcactacactattcaacattcattccaaagggtcaaaggattaaaaaatggctgacaa
tggcaaagtgcaggaagtgagtgttccacaggtgaaactgtatcttaaaagtgtaactttggcattatctccacttgttcagtgaggaccaagagcaaga
tgggaagtcagtccaagttctgttcctcgcctaaatgattggtcagcatagagacaaagctgatgggaatcttgcatctaatgttgttctctggttctgt
caggatcttcagagaaaaccagtttgacaacacaccatttaaagcatggaagttctgctcttggcctaacagctttaacctgtgtggaagtgaccatatt
aacataacatacaatgacaaagaggaaaatatgaaaatataaaaaggtcagcttatgagaaatacatatttttgctaacattcttccttgactagttgat
tactgtatgaataatgtatgtaccattgttgttttctgtggtggcggtattccaatatacgagtaaaaaaaatataacatggtagtattttgtacaaaaa
tacaatgttttaaaaagctttaaaaactatattctgataaaaacaattgcactatatacctgtattgtttaattctctataaggcattttgaaatttacc
aagagaaacttatttgaataaaaatatcttgaaatatttcataa
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: