XB-CLONE-3253169
Clone Name: IMAGE:7690259
|
Gene:
dnaaf2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX914637 [+]
agatcagtgaccctgagaaccggaaaaagtatgagcaagagatctacaaaatggaaaacgagagaggaatggacattaagtttatccacccaaagcccgg
gtatgtgttgctgagcagtgtcaatggggtgcagaaatgctaccttaatatctgcagcaatgatctgattcaaaagccagagttcaagcctggaaaagac
ggcgaaggcaaggctgggctgcattggtctctcccatacagtttatcaccgggcagggatgatctgagcaaagatgggagcaaacacgttatatatgatg
tagtgttccatccagacactctgcatattgccagcaagaatgggaaattcaaggggatagttgacagcacagctttggaagctgttgccagtcagtttaa
tgtcaccttagacaaagcaaatgtcaggacactcagcatgaaatataagggtgtaccaaatccgtctgtgttgcgaaaaccccttcctgaagcctcacca
aaatctagggaccttgaagaccccttatgtttcccttatccatatgatgttcctactgcagtaggcacagagaaaaaggaccagaagagagtgatacaaa
aagagtgtaaacaacatttagtgactccagaacaagaccccaacgtacaggttgctgctacccctaactatactgtgcggcacagatcatacgtggatct
gcaggatttccgggattctagagactctacaccaagtcctgtaccccaggaattggtaataaacggtgatttgcctttgcntaattctgccgcangagtc
aaccttcatatagctggcaaaaatttaagcctagagtctgagaa
3' EST: CX914636 [+]tttttttttttttttaagtatattttccctttattcaatgaagagcccatcagcagaatgaagcaacacaccaacacctgtttatgtgtaggaaaacaaa
aagtgcagttgtataggagcaaatatccttccgtttacattttttaaaaacaatccttcctgtgccttgatgaatcgctagttgcctatcggagggggtt
ggtttaatattgttgatttaattttgtgggcgacacttttctttttttattttaaaaaggtattcatagtaataacattcactgaaaacatccagtattt
tttggctgttctgtccagtgaatgtttgctgcaaaatttacactcagaagaccttttaaagacaacatatcaggaaaaaatccataaaactaaataaatc
tgtttaatcaaacttgcatatgtacaagagacgcttgcagtaagcagccatattggattgttgtctcagtagtgcgaaggaagtaaatattcttgtttca
caagtcaaatgcttaccgaggcagtaagctgtttttacatgtccatcacaagctactgtatggaaagagaataaacaatcagtccagttcaaacagcaat
gaattttggaactgaaaggggcaatgtgttgtgtgatcactgataacttggacactaccatctttgctgctgatttcttttaatatattgtttgaagatg
cactgttttgcagatgatctgagttatcaggcatatcatcttcatccaagtcactttcttgtgttgtttgtacacttgatttcaagcaggccatcttgtt
ttctgcattggagtcttgagcacagttgtctcctgggcagactagat
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: