XB-CLONE-3252675
Clone Name: IMAGE:7690853
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Gene:
ctcf
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161099 [+]
gcagagaatgtctgggccgccattttatgaagtagggaactggagcaattaaacggtgagctggttcttgtcaggaggcttcgtttctgtgtgttctcag
ataccttcttggccttcacatcggatttggccagacctgccgaaaaagctgaaaaaatacgagaaatagcctcaggaaagaggttgttaaaatggaaagt
gaaatggcggaagccgttgtggaggactcggaaaccttcatgaaaagaaaagagacaaagacatatcagaggcgtagggaaggtggtgtagatgaagata
actgtgtcatagtccaaagccagacagatataagtgaagtacctcatgatgtcaacagtaatgttcagatggtgatgatggagcaacttgatcccaccct
attacaaatgaagactgaagttatggaaggtgtggtttcacaagaaggtgatcctactgtggacgatacacaaataattacactacaggttgtcaatatg
gaagagcagccaattaatcttggagagcttcagcttgtccaagttccagtagctgttccgatggccacaacatcggtaggtgaacttcatgcagcttttg
aaaatgaggtatccaaagaaggccttcaggaaggggagcccatgatatgtcataccttacctcttccagagggtttccaagtagttaaggtaggggcaaa
tggagaggtagaaactctagaacaggcagagttacaacaacaagaagaacctggctggcaaaaggatcctgattatgtgccccctatcaaaaaaacaaag
aaaactaagaaaagcaaactgcgctacacagaagaaggaaaagatgttgatgtctctgtctatgattttgaagaagagcaacaggaaggactgctttctg
atgtgaatgcagaaaaggttgttggaaatatgaagcctccaaagccaacaaaaattaagaagaaaggtgtgaagaagacgttccagtgtgagttatgtag
ttacacctgtccaagacgttctaacctggatcgccacatgaaaagccacaccgatgaaaggccacacaagtgccacctatgcggaagggctttcaggact
gttactttgcttaggaaccacctcaatactcatacaggtactcggccacacaagtgtcctgactgtgacatggcatttgtaactagtggtgaattggtca
gacatcgtcgttataaacacacccatgaaaaaccattcaaatgttcgatgtgtgattatgccagtgttgaggtaagtaagctgaagcggcatattcgatc
acatactggagaacgtcccttccaatgcagcttgtgcagctatgccagcagggacacatacaagttgaaaagacacatgcgaacccattcaggtgaaaag
ccatacgaatgctacatttgccatgctcgttttacgcagagtgggaccatgaagatgcatattttgcagaaacacactgaaaacgtggccaaatttcatt
gtccccattgtgacacagtaattgcaaggaagagtgacttgggtgtccatttgcgaaagcaacattcctacattgagcaaggaaagaagtgtcgctactg
tgacacagtgttccatgagcgttatgcactaatccagcaccagaagtctcataaaaatgaaaaacgatttaaatgtgaccagtgtgaatatgcttgcaga
caggagcgacacatgattatgcacaaaaggacccatactggtgagaagccttatgcctgtagccattgtgataaaacatttagacagaaacagctgcttg
atatgcacttcaaaagataccatgacccaagttttgttcctgcagcatttgtgtgctccaagtgtgggaaaacatttactcgaaggaatacaatgtcaag
acatgcagataattgcactgggcctgatggtacagatggtgaaaatggaggagaaagtgaagtggttcacaaaaaagggaagcgtggtcgcaagagaaag
atgcgctccaagaaagaaggttcttctgacagtgaggataatgctgaacctgagctggaagacgatgatgatgaagatgaagacgatgaagatgagacac
ctgtagaaatagaagctgatcctgaaccagaagagccactgactccacttccaccccctgcaaagaagcgtagaggaagaccgcctggtaaagccaacca
agcaaagcaaaatgcagctgttattcaggtggatgatcacagcaacagagcaattgagaacataattgttcaggtgaagaaagaatccgacctagaagca
gaggggggtgtggaggctgctgtaccaacacctgcagtggaggcaccaaatggagaccttactccagaaatgatactgagcatgatggaccggtgatgca
ggcaaaacccattgccttgctttattgaactggctcgagctgtgtttaaatggctcattactattttatgagatctgataattttatttttaactttttg
ctttgtggatttttttttttttttaagatctgttttaccatgtctgtacaggaaaacctctttcaagtaaagcaaaacatactggctatccaaaattcaa
caatattttcaggccaatcatggaatcagttaagaggtttcctttagataaaatattctcttttgcggttaacccaaaatctgccagggaaaactaaaat
tttacttaagagtataaatttccctaaatgaattctacccagaatggctcgatcccagtctttatttacatggctttaattaatttaaaaaaaaaaaaaa
a
5' EST: CX915496 [+]cagagaatgtctgggccgccattttatgaagtagggaactggagcaattaaacggtgagctggttcttgtcaggaggcttcgtttctgtgtgttctcaga
taccttcttggccttcacatcggatttggccagacctgccgaaaaagctgaaaaaatacgagaaatagcctcaggaaagaggttgttaaaatggaaagtg
aaatggcggaagccgttgtggaggactcggaaaccttcatgaaaagaaaagagacaaagacatatcagaggcgtagggaaggtggtgtagatgaagataa
ctgtgtcatagtccaaagccagacagatataagtgaagtacctcatgatgtcaacagtaatgttcagatggtgatgatggagcaacttgatcccacccta
ttacaaatgaagactgaagttatggaaggtgtggtttcacaagaaggtgatcctactgtggacgatacacaaataattacactacaggttgtcaatatgg
aagagcagccaattaatcttggagagcttcagcttgtccaagttccagtagctgttccgatggccacaacatcggtaggtgaacttcatgcagcttttga
aaatgaggtatccaaagaaggccttcaggaaggggagcccatgatatgtcataccttacctcttccagagggtttccaagtagttaaggtaggggcaaat
ggagaggtagaaactctagaacaggcagagttaccacaatcagaagaacctggctggcaaaggatcctgattatgtgccccctatcaaaaaaacaaagaa
a
3' EST: CX915495 [+]tttttttttttttttaaattaattaaagccatgtaaataaagactgggatcgagccattctgggtagaattcatttagggaaatttatactcttaagtaa
aattttagttttccctggcagattttgggttaaccgcaaaagagaatattttatctaaaggaaacctcttaactgattccatgattggcctgaaaatatt
gttgaattttggatagccagtatgttttgctttacttgaaagaggttttcctgtacagacatggtaaaacagatcttaaaaaaaaaaaaaaatccacaaa
gcaaaaagttaaaaataaaattatcagatctcataaaatagtaatgagccatttaaacacagctcgagccagttcaataaagcaaggcaatgggttttgc
ctgcatcaccggtccatcatgctcagtatcatttctggagtaaggtctccatttggtgcctccactgcaggtgttggtacagcagcctccacacccccct
ctgcttctaggtcggattctttcttcacctgaacaattatgttctcaattgctctgttgctgtgatcatccacctgaataacagctgcattttgctttgc
ttggttggctttaccaggcggtcttcctctacgcttctttgcagggggtggaagtggagtcagtggctcttctggttcaggatcagcttctatttctaca
ggtgtctcatcttcatcgtcttcatcttcatcatcatcgtcttccagctcaggttcagcattatcctcactgtc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: