XB-CLONE-3252135
Clone Name: IMAGE:7691364
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Gene:
mki67
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC159125 [+]
ctggaaacggacgtgtcacggtacttggaggtccggctgctttgtcgttaatggactaaacggagaagggaaacgaaacttcagtgtccaggtaagattg
ccttattggaggatgccactttttggggaaattgttgtgataaaaaggaatggcacagatggtacccattttccacttactgcaactacttgtctgtttg
gaagaaaatcagaatgtgatatccgcattcagctaccacatgtctccaaagagcattgcagagttgaagtcaaagcaaacaaagaggtttttgtcatcaa
cttaagtaccgtcaatccgacacagttaaatggaatagcagttcagcaacctgaggtccttaaacatggagatgtaattactataattgatcgttctttc
agatttgaacaaccacagacgaaccctcgcagtcagtccacaggaggaaattctctaagtagcaagttggtcgaagatgaacagccatcaatcaaaggta
aagcgagtaagctcactcggaagtcagaaggaaatgtatctaggaccacccactctcgcctctctcttccaatatcaccacatagacgatcttcaagaga
gaagagagatttgtcaccttttggtgaattgtacgagatgcttaaaagtaaagagttaaatctagagcatgcaaaaacaccagcaaagctaaacatggag
aatggagatcatatcagtactcctgtcacaactagaaggagcgttggaaatgctaaagaaacgcaagagttgagcattggagaattgcagtttgaaggtc
aggaacttcatgttgaatcggcaaccttgtcagagaaaactgccagtgccttaaaaagtccatctagagggcgtcagtccactctgtcaaaacaagagca
acaaaggcttagtggtaaaagagtctctcctgccaaatctgagctgcttcttaaagatacagctagctttcaagataatgggcaaaaacagaccaatgac
aagctcactccaaatcggagacgtagtacttccaatgaacctgctcaagatgtgcaaatgacacctggaaaatctggaggttccgtttcacctcggtcca
gaaacatgtctccaaaatcagcatctcccaaaggcttgcacaagaacgttaaaggatcagctaaagtctcaaacccacgacaaaaacgacgtagttctga
gcttgatttgcctgagccaactccaaaaagaaaaagggtctcatttggaggacatctaagtccagagctttttgataaaaggcttcctcccaactctcct
cttaaaagaggtgcaacacctgctaggcggagcttatcacttgtcacgcctcttgcacttatgcgaaagagctttggtggtttcaggcaaaccgcaatta
aggaaacttttgagcctggcactgacttagcactcttcaagggatctccagccaagaagtcaccatccaagaggtcgccagccaagggggcatccccagc
caagaagtcgccggccaagaggtcgcccgccaagggggcatccccggccaagaggtcgcccgccaagggggcatccccggccaagaggtcgcccgccaag
ggggcatccccggccaagaggtcgcccgccaagggggcatccccggccaagaggtcgcccgccaagggggcttccccggccaagaggtcgcccgccaagg
gggcatccccggccaagaggtcgcccgccaagggggcatccccggccaagaggtcgccggctaaggcagctaccccggccaagaggtcgccagccaaggt
agctaccccggccaagaggtcgccagccaaggtagctaccccggccaagaggtcgccagccaaggtagctaccccggccaagaggtcgccagccaaggta
gctaccccggccaagaggtcgccagcaaaggcggcatccccagccaagaggtcgccggccaaggcagctaccccggccaagaggtcgccggcaaaggggg
catcccctgccaggaggtcgccggccaaggcagctacccctgccaggaggtcgccggccaaggcagctacccctgccaggaggtcgccagtcaaggcagc
tacccctgccaagaggtctcctgcctcagcaacccctgccaagaggtctcctgcttctacttatacaaaaggacgtttctcaatttcacgaattgataca
ccaccgcaaatagatgtccatgctcctaacactgagaaaaatgaactctctgcacaaacacccagaaaatcacggaagtcaatatcattaaagaaaacgc
caggaagaagaagtagaaaacttgaatcttttgaaattttgcgttccaggcgaagaagtggggcaactgaagctaacttgcttgttgcaaaatcatgggc
tgatgttgtaagaagcggtgttccaaaaaatcagaagaaatctgaaaagcatgctcgcaaagcagtacctaccaagagaaagccaaagccaaagacgcca
gcacgaagaattattgaccgaattggcacaggacatgctgattctcctgccacaatccttgttggaagagctcatacaagaactgtcgacctaactggat
atgttcctaaaattgttcaaaaccaagcaataaaaatcaccaatgaccaaaatgaaagctttacaggtttgagtgagttgtttaatacccctgcacatgt
gaaaccaaggagaagtggtcgctcaggaggccttccgatggaaactccgcaatctataattgttgatatgtcactgatgaagactccagaggaattaggt
gaaatggtggtttctccaattaatggtagccctgctacaacacagaagaaacagtacagtcgtgacgcagtatctcgtcttctaagtcctgtgtcgccag
catcagaagaacagatatccgaagcttccaaagacactcagttggttgtaaaagagacagccacattaaatgaaacccgttttataaaaaagggtgcaaa
ggcacagatgaaaaaaaaaaaaaaa
5' EST: CX916439 [+]ctggaaacggacgtgtcacggtacttggaggtccggctgctttgtcgttaatggactaaacggagaagggaaacgaaacttcagtgtccaggtaagattg
ccttattggaggatgccactttttggggaaattgttgtgataaaaaggaatggcacagatggtacccattttccacttactgcaactacttgtctgtttg
gaagaaaatcagaatgtgatatccgcattcagctaccacatgtctccaaagagcattgcagagttgaagtcaaagcaaacaaagaggtttttgtcatcaa
cttaagtaccgtcaatccgacacagttaaatggaatagcagttcagcaacctgaggtccttaaacatggagatgtaattactataattgatcgttctttc
agatttgaacaaccacagacgaaccctcgcagtcagtccacaggaggaaattctctaagtagcaagttggtcgaagatgaacagccatcaatcaaaggta
aagcgagtaagctcactcggaagtcagaaggaaatgtatctaggaccacccactctcgcctctctcttccaatatcaccacatagacgatcttcaagaga
gaagagagatttgtcaccttttggtgaattgtacgagatgcttaaaagtaaagagttaaatctagagcatgcaaaaacaccagcaaagctaaacatggag
aatggagatcatatcagtactcctgtcacaactagaaggagcgttggaaatgctaaagaaacgccagagttgagcattggagaaatgcagtttgaaggtc
aggaacttcatgttgaatcgngcaccttgtcagagaaactgccagtgccttaaaaagtcatct
3' EST: CX916438 [+]tttttttttttttttcatctgtgcctttgcacccttttttataaaacgggtttcatttaatgtggctgtctcttttacaaccaactgagtgtctttggaa
gcttcggatatctgttcttctgatgctggcgacacaggacttagaagacgagatactgcgtcacgactgtactgtttcttctgtgttgtagcagggctac
cattaattggagaaaccaccatttcacctaattcctctggagtcttcatcagtgacatatcaacaattatagattgcggagtttccatcggaaggcctcc
tgagcgaccacttctccttggtttcacatgtgcaggggtattaaacaactcactcaaacctgtaaagctttcattttggtcattggtgatttttattgct
tggttttgaacaattttaggaacatatccagttaggtcgacagttcttgtatgagctcttccaacaaggattgtggcaggagaatcagcatgtcctgtgc
caattcggtcaataattcttcgtgctggcgtctttggctttggctttctcttggtaggtactgctttgcgagcatgcttttcagatttcttctgattttt
tggaacaccgcttcttacaacatcagcccatgattttgcaacaagcaagttagcttcagttgccccacttcttcgcctggaacgcaaaatttcaaaagat
tcaagttttctacttcttcttcctggcgttttctttaatgatattgacttccgtgattttctgggtgtttgtgcagagagttcatttttctcagtgttag
gagcatggacatctatttgc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: