XB-CLONE-3252093
Clone Name: IMAGE:7691324
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Gene:
ago4
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ago1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC154703 [+]
GGCAGCTGCAACAGAACAGGGAGACAGTAGCCACATTACATTGTACATAAAACCAGATATTAAGCTGTGTGGATGGGATGGAAGCTGGACCTTCTTCTGC
AGCCCCGAGTGGCTATGTTCCACCCCTGCAGCAAGTGTTCCAAGCCCCACGAAGGCCAGGAATTGGTACTGTGGGGAAACCTATTAAATTACTTGCCAAT
TACTTTGAGGTAGACATTCCCAAGATTGACGTCTATCACTATGAGGTGGATATCAAACCAGATAAATGTCCTCGCCGTGTAAACAGGGAAGTTGTTGAGT
ACATGGTACAGCACTTTAAACCACAAATTTTTGGAGACAGAAAGCCAGTGTATGATGGAAAGAAGAATATTTACACTGTCACAGCACTTCCTATTGGCCA
TGAACGGGTTGATTTTGAGGTGACTATCCCCGGAGAAGGGAAGGATAGAATTTTTAAGGTATCTATAAAGTGGGTGGCAGTGGTGAGTTGGAGGATGCTT
CATGAGGCACTTGGGAGTGGACGGATTCAGCTACCCCTGGAGTCTGTGCAAGCTTTGGATGTAGCAATGAGGCACTTGGCATCAATGAGGTATACCCCTG
TTGGACGATCTTTCTTCTCTCCACCTGAGGGCTACTATCACCCTCTTGGGGGTGGGCGGGAGGTTTGGTTTGGTTTCCATCAGTCTGTGCGTCCAGCCAT
GTGGAACATGATGCTCAATATTGATGTGTCAGCAACTGCCTTCTACAAAGCCCAGCCTGTCATAGAGTTCATGTGTGAAGTACTGGATATACGAAACATT
GATGAACAGCCAAAACCTCTCACAGATTCTCAGAGAGTTCGCTTCACAAAGGAGATCAAAGGCTTAAAAGTAGAAGTTACCCACTGTGGGCAGATGAAGA
GAAAATACAGGGTATGCAATGTTACCCGGAGGCCGGCTAGTCATCAGACGTTCCCCTTACAGTTGGAGAGTGGTCAGACTGTTGAATGCACAGTAGCACA
GTATTTTAAACAGAAATATAACCTACAGCTAAAGTATCCCCACCTTCCCTGTCTCCAGGTGGGCCAAGAGCAGAAACACACATACCTACCTCTGGAGGTA
AGGAAATACAGGTTTGCAACATTGTAGCTGGCCAGCGATGTATTAAGAAGCTGACAGACAATCAGACCTCCACTATGATCAAAGCTACAGCTCGATCTGC
TCCAGACAGACAAGAGGAGATCAGCAGGCTGATGAAGAATGCCAGTTACAATTTGGATCCATACATTCAGGAATTTGGGATCAAAGTCAAAGATGACATG
ACAGAGGTCACTGGGCGAGTCCTCCCAGCACCCATTCTTCAGTACGGAGGGAGAAACAGAGCAATTGCAACTCCAAACCAAGGTGTCTGGGACATGCGAG
GAAAGCAGTTCTACAATGGCATTGAGATAAAAGTCTGGGCAATTGCATGTTTCGCTCCACAGAAGCAATGCAGGGAAGAAGTGCTGAAAAATTTTACCGA
CCAACTCCGCAAAATCTCAAAAGATGCAGGAATGCCAATCCAGGGTCAGCCATGCTTCTGTAAATATGCACAGGGGGCAGACAGTGTGGAGCCCATGTTC
CGGCACTTGAAAAACACATATTCTGGCCTTCAGCTCATCATTGTTATTCTGCCAGGGAAAACACCTGTATATGCGGAAGTGAAACGTGTTGGGGATACAT
TGCTTGGGATGGCCACTCAGTGTGTGCAGGTGAAAAATGTAGTTAAGACTTCTCCTCAGACGCTCAGCAACCTCTGCCTGAAGATCAATGTCAAGCTGGG
TGGTATCAACAACATTTTAGTACCTCATCAACGGTCTGCGGTCTTCCAGCAGCCAGTTATATTTCTTGGAGCAGATGTTACACACCCACCTGCTGGTGAT
GGCAAGAAGCCCTCTATTACTGCGGTTGTTGGTAGCATGGATGCCCACCCAAGCCGGTACTGTGCCACTGTGAGAGTTCAGCGACCTCGACAGGAGATCA
TTGAGGACCTGTCTTATATGGTGAGGGAATTACTTATCCAGTTTTATAAGTCCACCCGCTTCAAACCAACTCGAATAATTTTCTATCGTGATGGGGTTCC
TGAAGGACAACTGCCCCAGATCTTGCATTATGAGTTGCTGGCAATTCGGGATGCCTGTATTAAATTGGAGAAGGACTATCAGCCTGGAATCACGTATATA
GTTGTGCAGAAACGTCACCACACGCGGCTGTTTTGCGCTGACCGCAGTGAGCGGATTGGAAAAAGTGGAAACATTCCAGCAGGAACAACTGTTGACACCA
ACATCACACACCCTTTTGAATTTGACTTTTACTTGTGCAGCCATGCTGGTATCCAGGGAACAAGCCGCCCCTCTCACTACTATGTGCTTTGGGATGACAA
CAGATTCACAGCAGATGAGCTGCAGATTCTCACTTACCAGCTTTGCCACACCTACGTGCGCTGTACACGCTCTGTCTCAATTCCTGCACCAGCCTATTAT
GCTCGATTGGTGGCATTTAGAGCAAGGTATCACTTGGTGGATAAGGAACATGACAGTGGAGAAGGCAGTCATATCTCTGGACAGAGCAATGGGAGGGACC
CACAGGCCTTGGCAAAAGCAGTGCAAGTTCACCAGGACACACTGCGCACAATGTATTTTGCCTGATGGGCTTCACAAAGCATTTTAAGTGGATACCATCT
ATTTTATGACTTTTTTTTTGCCCTAACGTGTACCTCGGTTGCTCAGAAGAGTTGGGGTGCCTGAGCACTACAGAAGAAGAGGTAACTCTATTAGAAAAAA
CTTAGACGCAAGTGAGTCCAGTGGGACCAACAAGGAGATCAGAGGACACTGCTGAAGATATATATATTTTGCATGTTTGGAGAGAAGACTTTTTAAAACC
ACATACATGGAGAGTCTTCATCCAAACAAAAAGGAAGTTTTGTTCCTGATGCCACGGCAAAATTTTACATCTTGATAACTTGAAATGAATTGTACAAGTA
CTGTGTATCAAGCTAAGCCCCTTTCTCCTCAAAAAAAGGAACGATCCGATATTTGGAGTGTGAACTAAAAAAAAAAAAAAA
5' EST: CX916501 [+]gcagctgcaacagaacagggagacagtagccacattacattgtacataaaaccagatattaagctgtgtggatgggatggaagctggaccttcttctgca
gccccgagtggctatgttccacccctgcagcaagtgttccaagccccacgaaggccaggaattggtactgtggggaaacctattaaattacttgccaatt
actttgaggtagacattcccaagattgacgtctatcactatgaggtggatatcaaaccagataaatgtcctcgccgtgtaaacagggaagttgttgagta
catggtacagcactttaaaccacaaatttttggagacagaaagccagtgtatgatggaaagaagaatatttacactgtcacagcacttcctattggccat
gaacgggttgattttgaggtgactatccccggagaagggaaggatagaatttttaaggtatctataaagtgggtggcagtggtgagttggaggatgcttc
atgaggcacttgggagtggacggattcagctacccctggagtctgtgcaagctttggatgtagcaatgaggcacttggcatcaatgaggtatacccctgt
tggacgatctttcttctctccacctgagggctactatcacc
3' EST: CX916500 [+]cacactccaaatatcggatcgttcctttttttgaggagaaaggggcttagcttgatacacagtacttgtacaattcatttcaagttatcaagatgtaaaa
ttttgccgtggcatcaggaacaaaacttcctttttgtttggatgaagactctccatgtatgtggttttaaaaagtcttctctccaaacatgcaaaatata
tatatcttcagcagtgtcctctgatctccttgttggtcccactggactcacttgcgtctaagttttttctaatagagttacctcttcttctgtagtgctc
aggcaccccaactcttctgagcaaccgaggtacacgttagggcaaaaaaaaagtcataaaatagatggtatccacttaaaatgctttgtgaagcccatca
ggcaaaatacattgtgcgcagtgtgtcctggtgaacttgcactgcttttgccaaggcctgtgggtccctcccattgctctgtccagagatatgactgcct
tctccactgtcatgttccttatccaccaagtgataccttgctctaaatgccaccaatcgagcataataggctggtgcaggaattg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: