XB-CLONE-3252089
Clone Name: IMAGE:7691134
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Gene:
ankhd1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121940 [+]
GTTTACAAGAAGTTAATGGCTAGGAAACGGGTTGCTTTGCTTATTTTTTTAATTACTTCAGATGTCTGCTTTTGAGGAGTTCGCGTTTTGGAAGTCGGCA
CAGTAAGCAGACAAAGTTTAAAGACTCGTCGTTTCGCTCTTCAGATCTCGGTCTTTTTCCACGCTTGACGGGAGCTATGCAGAATGCTGTGACCGGGACG
GACATGCTCACGGAAGGCTTTGAAGATGAGATCGACTCGGTAACTCCGCGCTCGCCAGTGACGGAGCTGGGAAGTGAAGCCGGTGTCGGACTCTCCGCTG
CCAAGGTTCGCCTTTTTGGGGGCGCGTCGACTGGAAACGAGAGACTGGATTTTAGGTTGGCTGCTGCCGCTGTGCTCTCCTCCGGAACTGGCTCTGGCAG
TGACGAGGACGAAGTGTCTGAAGTTGAGTCGTTTATTTTGGACCAAGAGGACTTGGACAATCCAGTGCTTAAAACTGCATCTGAACTCTTCCTGTCTACT
GCTGCTGAAGGAACAGACTTTAGAACTGTTGATCCTGAGACACAGGCACGTCTAGAAGCTTTATTAGAAGCTGCAGGAATTGGTAAATTGTCCACTGCTG
ATGGTAAAGCCTTTGCAGATCCTGAAGTACTTAGAAGATTGACATCGTCTGTGAGCTGTGCTTTGGATGAAGCTGCGGCTGCACTAACTCGGATGAGAGC
AGACAATACTCTCAGCACAGGACAAGTAGACAATCGGAGTCTCGCAGAAGCCTGTTCAGATGGAGATGTTAATGCTGTTCGTAAACTGCTTGATGAAGGT
AGAAGTGTAAATGAACACACGGAGGAGGGAGAAAGTCTTCTCTGCTTGGCCTGTTCTGCTGGATATTATGAGTTGGCACAAGTGTTGCTTGCTATGCATG
CCAATGTTGAAGACAGAGGAAATAAAGGTGACATTACACCTTTAATGGCAGCTGCCAGTGGTGGTTTTGTCGATATTGTTAAACTGTTATTGGCTCATTC
TGCAGATGTAAATGCACAGTCATCGACTGGTAACACAGCGCTTACGTATGCTTGTGCTGGTGGCTTTGTTGATGTAGTTAAAGTTTTGCTGAAAGAAGGT
GCTAATATAGAGGATCACAATGAAAATGGGCACACCCCTTTAATGGAAGCAGCTAGTGCCGGGCATGTAGAGGTAGCAAGGGTCTTGCTAGAATTTGGAG
CAGGCATCAACACACATTCAAATGAATTCAAAGAAAGTGCACTTACCCTGGCATGCTACAAAGGCCATTTAGACATGGTTCGATTTTTGCTTGAAGCTGG
AGCTGATCAAGAGCATAAAACTGATGAGATGCACACAGCCTTAATGGAGGCTTGCATGGATGGCCATGTCGAAGTAGCGCGTTTACTGCTAGATAGTGGT
GCTCAAGTAAACATGCCTGCAGATTCTTTTGAGTCTCCTTTGACTTTGGCAGCCTGTGGAGGTCATGTGGAACTTGCTGCCCTTCTGATAGAAAGAGGGG
CTAATTTGGAGGAAGTAAATGATGAAGGTTATACACCTTTAATGGAAGCCGCACGAGAGGGCCATGAAGAAATGGTGGCACTCCTCCTTGCACAAGGGGC
CAACATAAATGCACAGACAGAAGAAACACAAGAAACCGCTCTAACTTTGGCGTGCTGCGGTGGATTTTCAGAAGTGTCCGATTTTCTCATAAAAGCAGGA
GCAGACATAGAACTTGGCTGTTCTACGCCGCTTATGGAAGCTGCTCAGGAGGGGCATCTAGAGCTGGTGAAATATTTGTTGGCAGCAGGTGCAAATGTAC
AAGCAACAACTGCTACAGGGGATACAGCTCTGACATACGCATGTGAAAATGGTCACACAGATGTTGCAGATGTGTTGTTGCAGAGTGGTTCCGATTTAGA
ACATGAATCTGAAGGTGGAAGAACTCCACTAATGAAAGCTGCAAGAGCTGGCCATCTTTGCACAGTTCAGTTTCTCACTAGCAAAGGTGCTGACATTAAT
AGAGCTACTGCAAATAATGATCACACTGTGGTATCCCTTGCATGTGCTGGAGGTCATCTTGCAGTTGTTGAGTTACTTCTAGCACACGGTGCAGACCCAA
CTCATCGTTTGAAGGATGGTTCTACCATGCTGATAGAAGCAGCAAAAGGTGGACATACTAATGTTGTTTCCTATTTGCTGGATTTTCCAAATAACATTTC
GTCAGTCCCAGCTACAGATATGTCTCAATTGACTCCACCATCTCATGATCAACCTCCGATTCCACGTGTACCTATGAATACTTTGGCAATGGTGGTACCC
CCTCAAGAGCCTGATAAAGCTCCTGAACAATATGCAACAGAAAGAGCAAAATTAAAGCAAAAGCCAGATAACCTGCATGCATCTGATCAGGATCTGCGAT
CACCTTTTCATCCATGTCAGCTGTTGGAATGTATAGTAGAAGAAACCGAAGGAAAGCTTAATGAACTTGGTCAAAGAATTAGTGCTATTGAAAAGGCACA
GCTCAAATCATTAGAACTAATTCAAGGTGAACCTCTGAATAAAGATAAAATTGAAGAGCTTAAAAAGAACAGAGAAGAACAAGTCCAGAAGAAAAAGAAA
ATTTTAAAGGAGCTTCAAAAAGTAGAAAGGCAATTGCAAATGAAAACACAACAGCAGTTCACCAAAGAATATTTGGAGTCGAGCCCCCCTAAAGATCCAA
CAGTTACGCATCAGTGCCCTCTTAAAAGCGCAATCCCACCATTAGATTCAAATGATGATTTGTCAGAGGAGAACTGTGTGAAATTGTCTCAAGTTGGAAA
TATCTTGGCTGATGATGGTGATCATAAGTTATTGCCTCAGCCAGATGTTGAACAGATGGGATTTGTACCTATTCATCCATCAGCAACATCATATTCTGAA
TTATCCAATGAAATTGAATTCTGTGAAATGAATTCTCTTCAGCCACCAATATATGGAAACCAACCATTACTAGAACAGCAACAAGCGGCTAATCAGGTAC
TTTTATCTCAAGCACCAGATGCATTAATGGTTGCCTCTCCAGCACAGACCCTAACTGAAACCCTTGATGACATCATGGCTGCTGTGAGTAGTAGAGTGCC
TTCTATACCTGATGAATCCTCACTTCCCACTCCATCTTCGCTAAGTCTTCAGTCTACTCCAACGCCACAACCGTTGCTCTCTACTTTCCCCTCAATAGAT
ATTGATGCGCATACTGAAAGTAATCATGATACCGCATTAACCCTTGGATGTGCTGGAGGTCACGAAGAGCTAGTGTCTGTGTTAATTACACGTTCTGCAA
ATATTGAACACAGAGACAAAAAAGGTTTCACCCCCCTTATTTTGGCTGCTACTGCTGGACATGTTGGAGTGGTAGAGATTCTGTTGGATAAAGGAGCTGA
CATTGAAGCACAGTCAGAACGTACTAAGGATACTCCTCTCTCGCTTGCATGCTCTGGTGGGCGTCAAGAGGTGGTTGAATTGCTTTTGGCTCGGGGTGCT
AACAAAGAGCACAGAAATGTTTCAGACTATACCCCACTCAGTTTGGCTGCATCTGGTGGATATGTCAATATTATTAAAATCCTTCTTAATGCTGGTGCAG
AAATTAACTCAAGAACTGGCAGTAAACTTGGGATTTCCCCTTTGATGTTGGCTGCAATGAATGGACATGTCCCTGCAGTTAAACTGCTTCTTGATATGGG
TTCTGACATCAATGCTCAGATTGAAACAAATCGTAATACTGCACTTACTTTGGCATGCTTTCAAGGTAGAGCAGAAGTTGTGAGCCTTCTCTTAGATAGA
AAAGCCAACGTTGAACATAGAGCTAAGACTGGACTTACACCGCTAATGGAGGCAGCCTCAGGGGGTTATGCAGAAGTGGGAAGAGTTCTTCTAGACAAAG
GAGCTGATGTAAATGCACCTCCAGTGCCTTCATCAAGGGATACTGCTTTAACAATTGCTGCAGACAAAGGCCATTACAAATTCTGTGAACTGCTAATCAG
TAGAGGTTCACATATTGATGTTCGAAACAAAAAAGGAAACACACCACTTTGGCTGGCTGCAAATGGTGGTCATTTTGATGTAGTACAGCTTCTTGTGCAG
TCTGGGGCTGATGTTGACGCAGCTGATAATAGAAAGATCACTCCTCTTATGTCTGCATTTCGAAAGGGTCACTTAAAAGTGATACAGTTTTTGGTTAAAG
AAGTAAACCAATTTCCATCTGATATTGAGTGCATGCGCTACATTGCCACTATAACTGATAAGGACCTTTTGAAGAAATGCCATCAATGTATGGAAACAAT
TGTAAAAGCAAAAGACCAGCAGGCTGCAGAAGCAAACAAAAATGCAAGCATCCTCCTTAAAGAGCTAGATTTAGAGAAGTCAAGAGAAGAAAGCAGAAAA
CAAGCTCTTGCTGCAAAAAGAGAAAAGCGTAAGGAGAAAAAAAAAAAAAAAA
5' EST: CX916509 [+]tttacaagaagttaatggctaggaaacgggttgctttgcttatttttttaattacttcagatgtctgcttttgaggagttcgcgttttggaagtcggcac
agtaagcagacaaagtttaaagactcgtcgtttcgctcttcagatctcggtctttttccacgcttgacgggagctatgcagaatgctgtgaccgggacgg
acatgctcacggaaggctttgaagatgagatcgactcggtaactccgcgctcgccagtgacggagctgggaagtgaagccggtgtcggactctccgctgc
caaggttcgcctttttgggggcgcgtcgactggaaacgagagactggattttaggttggctgctgccgctgtgctctcctccggaactggctctggcagt
gacgaggacgaagtgtctgaagttgagtcgtttattttggaccaagaggacttggacaatccagtgcttaaaactgcatctgaactcttcctgtctactg
ctgctgaaggaacagactttagaactgttgatcctgagacacaggcacgtctagaagctttattagaagctgcaggaattggtaaattgtccactgctga
tggtaaagcctttgcagatcctgaagtacttagaagattgacatcgtctgtgagctgtgctttggatgaagctgcggctgcactaactcggatgagagca
gacaatactctcagcacaggacaagtagacaatcggagtctcgcagaagcctgttcagatggagatgttaatgctgttcgtaaactgcttgatgaaggta
gaagtgtaaatg
3' EST: CX916508 [+]ccttacgcttttctctttttgcagcaagagcttgttttctgctttcttctcttgacttctctaaatctagctctttaaggaggatgcttgcatttttgtt
tgcttctgcagcctgctggtcttttgcttttacaattgtttccatacattgatggcatttcttcaaaaggtccttatcagttatagtggcaatgtagcgc
atgcactcaatatcagatggaaattggtttacttctttaaccaaaaactgtatcacttttaagtgaccctttcgaaatgcagacataagaggagtgatct
ttctattatcagctgcgtcaacatcagccccagactgcacaagaagctgtactacatcaaaatgaccaccatttgcagccagccaaagtggtgtgtttcc
ttttttgtttcgaacatcaatatgtgaacctctactgattagcagttcacagaatttgtaatggcctttgtctgcagcaattgttaaagcagtatccctt
gatgaaggcactggaggtgcatttacatcagctcctttgtctagaagaactcttcccacttctgcataaccccctgaggctgcctccattagcggtgtaa
gtccagtcttagctctatgttcaacgttggcttttctatctaagagaaggctcacaacttctgctctaccttgaaagcatgccaaagtaagtgcagtatt
acgatttgtttcaatctgagcattgatgtcagaacccatatcaagaagcagtttaactgcagggacatgtccattcattgcagcccacatcaaaggggaa
at
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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